(Circular) (Six-base) MAPSORT of: pFN21ASDA0198.seq Check: 200 from: 1 to: 6217 pFN21ASDA0198 6217 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4027 278 Size: 53 83 186 936 2491 2468 Fragments arranged by size: 2491 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3560 1937 Size: 1623 4594 Acc65I G'GTAC_C Cuts at: 3538 3538 Size: 6217 AclI AA'CG_TT Cuts at: 1563 4344 4717 1563 Size: 2781 373 3063 Fragments arranged by size: 3063 2781 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2567 4280 5664 5932 1632 Size: 935 1713 1384 268 1917 Fragments arranged by size: 1917 1713 1384 935 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6020 AflIII A'CryG_T Cuts at: 1203 2726 2787 5116 6093 1203 Size: 1523 61 2329 977 1327 Fragments arranged by size: 2329 1523 1327 977 61 AgeI A'CCGG_T Cuts at: 5048 5048 Size: 6217 AhdI GACnn_n'nnGTC Cuts at: 4946 4946 Size: 6217 AleI CACnn'nnGTG Cuts at: 1166 2598 2948 1166 Size: 1432 350 4435 Fragments arranged by size: 4435 1432 350 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2966 3500 1184 Size: 1782 534 3901 Fragments arranged by size: 3901 1782 534 AlwNI CAG_nnn'CTG Cuts at: 2533 2758 3367 3410 3960 5532 2533 Size: 225 609 43 550 1572 3218 Fragments arranged by size: 3218 1572 609 550 225 43 ApaLI G'TGCA_C Cuts at: 2657 2966 4274 5430 2657 Size: 309 1308 1156 3444 Fragments arranged by size: 3444 1308 1156 309 ApoI r'AATT_y Cuts at: 1493 2151 3526 3752 3904 6013 1493 Size: 658 1375 226 152 2109 1697 Fragments arranged by size: 2109 1697 1375 658 226 152 AseI AT'TA_AT Cuts at: 160 1032 4771 160 Size: 872 3739 1606 Fragments arranged by size: 3739 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6217 AvaI C'yCGr_G Cuts at: 1870 1943 2109 2862 3542 1870 Size: 73 166 753 680 4545 Fragments arranged by size: 4545 753 680 166 73 BamHI G'GATC_C Cuts at: 3547 3914 3547 Size: 367 5850 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2451 2576 2646 2918 3538 Size: 325 217 627 664 125 70 272 620 Cuts at: 3538 4993 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 664 627 620 325 272 217 125 70 BanII G_rGCy'C Cuts at: 1391 2781 2843 3221 3536 1391 Size: 1390 62 378 315 4072 Fragments arranged by size: 4072 1390 378 315 62 BbeI G_GCGC'C Cuts at: 2455 2455 Size: 6217 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3448 928 Size: 971 1549 3697 Fragments arranged by size: 3697 1549 971 BbvCI CC'TCA_GC Cuts at: 2186 2235 2489 3168 3354 2186 Size: 49 254 679 186 5049 Fragments arranged by size: 5049 679 254 186 49 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4408 4442 1903 Size: 34 2471 34 3678 Fragments arranged by size: 3678 2471 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4109 5319 4109 Size: 1210 5007 BclI T'GATC_A Cuts at: 1548 1548 Size: 6217 BfrBI ATG'CAT Cuts at: 2467 2467 Size: 6217 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4828 136 Size: 107 122 71 631 605 3156 1525 Fragments arranged by size: 3156 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6212 6212 Size: 6217 BlpI GC'TnA_GC Cuts at: 2837 3030 3387 3634 2837 Size: 193 357 247 5420 Fragments arranged by size: 5420 357 247 193 Bme1580I G_kGCm'C Cuts at: 1790 2661 2698 2923 2970 4278 5434 1790 Size: 871 37 225 47 1308 1156 2573 Fragments arranged by size: 2573 1308 1156 871 225 47 37 BmgBI CAC'GTC Cuts at: 1318 3462 1318 Size: 2144 4073 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3284 3948 4906 Size: 416 53 83 399 24 1867 664 958 Cuts at: 4906 442 Size: 1753 Fragments arranged by size: 1867 1753 958 664 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6217 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3015 3015 Size: 6217 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4877 1239 Size: 3638 2579 Bpu10I CC'TnA_GC Cuts at: 2186 2235 2489 3168 3354 2186 Size: 49 254 679 186 5049 Fragments arranged by size: 5049 679 254 186 49 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2747 3378 4342 5207 5505 2747 Size: 631 964 865 298 3459 Fragments arranged by size: 3459 964 865 631 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2212 4880 882 Size: 617 713 2668 2219 Fragments arranged by size: 2668 2219 713 617 BsaAI yAC'GTr Cuts at: 493 1204 3514 4008 493 Size: 711 2310 494 2702 Fragments arranged by size: 2702 2310 711 494 BsaBI GATnn'nnATC Cuts at: 6211 6211 Size: 6217 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2452 4024 4406 5041 Size: 53 83 186 936 919 1572 382 635 Cuts at: 5041 275 Size: 1451 Fragments arranged by size: 1572 1451 936 919 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4650 5048 5322 5469 5880 4650 Size: 398 274 147 411 4987 Fragments arranged by size: 4987 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3318 3348 1178 Size: 30 165 30 1915 30 4047 Fragments arranged by size: 4047 1915 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2090 1186 Size: 904 5313 BseYI C'CCAG_C Cuts at: 2440 2697 2939 3025 3153 3410 5420 5916 2440 Size: 257 242 86 128 257 2010 496 2741 Fragments arranged by size: 2741 2010 496 257 257 242 128 86 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2225 3422 1163 Size: 573 489 1197 3958 Fragments arranged by size: 3958 1197 573 489 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2614 3667 4428 5456 1540 Size: 275 186 613 1053 761 1028 2301 Fragments arranged by size: 2301 1053 1028 761 613 275 186 BsiHKAI G_wGCw'C Cuts at: 2544 2661 2970 4278 4363 5434 2544 Size: 117 309 1308 85 1071 3327 Fragments arranged by size: 3327 1308 1071 309 117 85 BsmI GAATG_Cn' Cuts at: 3503 3728 3821 3503 Size: 225 93 5899 Fragments arranged by size: 5899 225 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2544 2661 2698 2781 2843 2923 2970 Size: 399 754 117 37 83 62 80 47 Cuts at: 2970 3221 3536 4278 4363 5434 1391 Size: 251 315 742 85 1071 2174 Fragments arranged by size: 2174 1071 754 742 399 315 251 117 85 83 80 62 47 37 BspEI T'CCGG_A Cuts at: 5880 5880 Size: 6217 BspHI T'CATG_A Cuts at: 4106 5836 4106 Size: 1730 4487 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3303 3572 844 Size: 2459 269 3489 Fragments arranged by size: 3489 2459 269 BsrBI CCG'CTC Cuts at: 1394 1823 4104 1394 Size: 429 2281 3507 Fragments arranged by size: 3507 2281 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4712 4886 65 Size: 1183 3464 174 1396 Fragments arranged by size: 3464 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2448 2613 3172 4861 5048 1700 Size: 110 638 165 559 1689 187 2869 Fragments arranged by size: 2869 1689 638 559 187 165 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6217 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6217 BssSI C'ACGA_G Cuts at: 4277 5289 4277 Size: 1012 5205 BstAPI GCAn_nnn'nTGC Cuts at: 794 2502 2576 2758 3209 3445 794 Size: 1708 74 182 451 236 3566 Fragments arranged by size: 3566 1708 451 236 182 74 BstBI TT'CG_AA Cuts at: 2007 5021 2007 Size: 3014 3203 BstEII G'GTnAC_C Cuts at: 1184 2483 1184 Size: 1299 4918 BstXI CCAn_nnnn'nTGG Cuts at: 2705 2705 Size: 6217 BstYI r'GATC_y Cuts at: 1972 3056 3547 3914 4299 4316 5768 5854 5866 Size: 1084 491 367 385 17 1452 86 12 Cuts at: 5866 6212 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1084 491 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2781 2904 2982 513 Size: 552 1716 123 78 3748 Fragments arranged by size: 3748 1716 552 123 78 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5478 Fragments arranged by size: 5478 683 56 BtsI GCAGTG_nn' Cuts at: 2404 2605 3203 3733 4527 4547 2404 Size: 201 598 530 794 20 4074 Fragments arranged by size: 4074 794 598 530 201 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6217 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6182 DraI TTT'AAA Cuts at: 3521 3521 Size: 6217 DraIII CAC_nnn'GTG Cuts at: 2653 2653 Size: 6217 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5224 809 Size: 507 3908 1802 Fragments arranged by size: 3908 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2289 2460 2707 Size: 54 1425 50 275 18 459 171 247 Cuts at: 2707 3664 4553 6182 8 Size: 957 889 1629 43 Fragments arranged by size: 1629 1425 957 889 459 275 247 171 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3664 1537 Size: 275 1852 4090 Fragments arranged by size: 4090 1852 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2735 2826 3025 4147 1353 Size: 1382 91 199 1122 3423 Fragments arranged by size: 3423 1382 1122 199 91 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2138 3128 4804 5178 5324 387 Size: 1064 138 549 990 1676 374 146 1280 Fragments arranged by size: 1676 1280 1064 990 549 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2567 4280 4877 5664 5932 1239 Size: 393 935 1713 597 787 268 1524 Fragments arranged by size: 1713 1524 935 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 3233 3233 Size: 6217 EcoO109I rG'GnC_Cy Cuts at: 2398 2530 3238 2398 Size: 132 708 5377 Fragments arranged by size: 5377 708 132 EcoRI G'AATT_C Cuts at: 3526 3904 3526 Size: 378 5839 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3224 3224 Size: 6217 FspI TGC'GCA Cuts at: 4723 4723 Size: 6217 HaeII r_GCGC'y Cuts at: 1678 2455 5364 1678 Size: 777 2909 2531 Fragments arranged by size: 2909 2531 777 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2368 2400 3318 3350 4858 Size: 32 135 32 796 32 918 32 1508 Cuts at: 4858 4890 4932 4964 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1508 918 796 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3561 3807 669 Size: 988 281 1623 246 3079 Fragments arranged by size: 3079 1623 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6217 HpaI GTT'AAC Cuts at: 3807 3807 Size: 6217 KasI G'GCGC_C Cuts at: 2451 2451 Size: 6217 KpnI G_GTAC'C Cuts at: 3542 3542 Size: 6217 MfeI C'AATT_G Cuts at: 3816 3816 Size: 6217 MluI A'CGCG_T Cuts at: 2726 6093 2726 Size: 3367 2850 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5331 5515 1305 Size: 4026 184 2007 Fragments arranged by size: 4026 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2291 2462 10 Size: 54 1425 343 459 171 3765 Fragments arranged by size: 3765 1425 459 343 171 54 MslI CAynn'nnrTG Cuts at: 518 1166 2598 2909 2948 2987 4175 4534 4693 Size: 648 1432 311 39 39 1188 359 159 Cuts at: 4693 518 Size: 2042 Fragments arranged by size: 2042 1432 1188 648 359 311 159 39 39 MspA1I CmG'CkG Cuts at: 1606 2239 2481 2493 2701 2805 3146 3182 3633 Size: 633 242 12 208 104 341 36 451 Cuts at: 3633 4310 5458 5703 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 633 451 341 245 242 208 104 36 12 NaeI GCC'GGC Cuts at: 3174 3174 Size: 6217 NarI GG'CG_CC Cuts at: 2452 2452 Size: 6217 NcoI C'CATG_G Cuts at: 513 1065 2904 2982 513 Size: 552 1839 78 3748 Fragments arranged by size: 3748 1839 552 78 NdeI CA'TA_TG Cuts at: 387 387 Size: 6217 NgoMIV G'CCGG_C Cuts at: 3172 3172 Size: 6217 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6217 NotI GC'GGCC_GC Cuts at: 3664 3664 Size: 6217 NsiI A_TGCA'T Cuts at: 2469 2469 Size: 6217 NspI r_CATG'y Cuts at: 1132 1229 2471 2791 2899 2914 3575 5120 1132 Size: 97 1242 320 108 15 661 1545 2229 Fragments arranged by size: 2229 1545 1242 661 320 108 97 15 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6217 PciI A'CATG_T Cuts at: 2787 5116 2787 Size: 2329 3888 PflMI CCAn_nnn'nTGG Cuts at: 1108 3274 3410 1108 Size: 2166 136 3915 Fragments arranged by size: 3915 2166 136 PfoI T'CCnGG_A Cuts at: 3121 3121 Size: 6217 PmeI GTTT'AAAC Cuts at: 3521 3521 Size: 6217 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4286 4318 5799 5831 4286 Size: 32 1481 32 4672 Fragments arranged by size: 4672 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6217 PsiI TTA'TAA Cuts at: 3787 3787 Size: 6217 PstI C_TGCA'G Cuts at: 830 2376 2511 2810 3569 830 Size: 1546 135 299 759 3478 Fragments arranged by size: 3478 1546 759 299 135 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6217 PvuII CAG'CTG Cuts at: 2239 2493 2805 3182 2239 Size: 254 312 377 5274 Fragments arranged by size: 5274 377 312 254 SalI G'TCGA_C Cuts at: 1936 3559 1936 Size: 1623 4594 SapI GCTCTTCn'nnn_ Cuts at: 2735 2826 2735 Size: 91 6126 SbfI CC_TGCA'GG Cuts at: 3569 3569 Size: 6217 ScaI AGT'ACT Cuts at: 2690 4465 6177 2690 Size: 1775 1712 2730 Fragments arranged by size: 2730 1775 1712 SfcI C'TryA_G Cuts at: 826 1045 2372 2507 2806 3565 4700 5381 5572 Size: 219 1327 135 299 759 1135 681 191 Cuts at: 5572 826 Size: 1471 Fragments arranged by size: 1471 1327 1135 759 681 299 219 191 135 SfoI GGC'GCC Cuts at: 2453 2453 Size: 6217 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6217 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6217 SmaI CCC'GGG Cuts at: 1872 3544 1872 Size: 1672 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2762 3393 4321 5222 5484 820 Size: 197 926 819 631 928 901 262 1553 Fragments arranged by size: 1553 928 926 901 819 631 262 197 SnaBI TAC'GTA Cuts at: 493 3514 4008 493 Size: 3021 494 2702 Fragments arranged by size: 3021 2702 494 SpeI A'CTAG_T Cuts at: 152 2095 152 Size: 1943 4274 SphI G_CATG'C Cuts at: 1132 2471 3575 1132 Size: 1339 1104 3774 Fragments arranged by size: 3774 1339 1104 SspI AAT'ATT Cuts at: 5 52 4141 5 Size: 47 4089 2081 Fragments arranged by size: 4089 2081 47 StuI AGG'CCT Cuts at: 1517 2107 2191 2796 2885 3330 1517 Size: 590 84 605 89 445 4404 Fragments arranged by size: 4404 605 590 445 89 84 StyI C'CwwG_G Cuts at: 513 1065 2580 2880 2904 2982 3656 513 Size: 552 1515 300 24 78 674 3074 Fragments arranged by size: 3074 1515 674 552 300 78 24 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2309 4244 4261 4414 4599 5044 325 Size: 1255 729 1935 17 153 185 445 1498 Fragments arranged by size: 1935 1498 1255 729 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2325 2688 4463 Size: 274 80 33 51 1443 348 363 1775 Cuts at: 4463 6175 96 Size: 1712 138 Fragments arranged by size: 1775 1712 1443 363 348 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2666 2698 6177 6209 2666 Size: 32 3479 32 2674 Fragments arranged by size: 3479 2674 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6217 XbaI T'CTAG_A Cuts at: 3553 3553 Size: 6217 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3094 3328 3094 Size: 234 5983 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6217 XmaI C'CCGG_G Cuts at: 1870 3542 1870 Size: 1672 4545 XmnI GAAnn'nnTTC Cuts at: 4346 4346 Size: 6217 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4025 276 Size: 53 83 186 936 2491 2468 Fragments arranged by size: 2491 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FseI FspAI NruI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI