(Circular) (Six-base) MAPSORT of: pFN21ASDA0193.seq Check: 27 from: 1 to: 5971 pFN21ASDA0193 5971 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3781 278 Size: 53 83 186 936 2245 2468 Fragments arranged by size: 2468 2245 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3314 1937 Size: 1377 4594 Acc65I G'GTAC_C Cuts at: 3292 3292 Size: 5971 AclI AA'CG_TT Cuts at: 1563 4098 4471 1563 Size: 2535 373 3063 Fragments arranged by size: 3063 2535 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4034 5418 5686 1632 Size: 2402 1384 268 1917 Fragments arranged by size: 2402 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5774 AflIII A'CryG_T Cuts at: 1203 4870 5847 1203 Size: 3667 977 1327 Fragments arranged by size: 3667 1327 977 AgeI A'CCGG_T Cuts at: 4802 4802 Size: 5971 AhdI GACnn_n'nnGTC Cuts at: 4700 4700 Size: 5971 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5971 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2485 2789 1184 Size: 1301 304 4366 Fragments arranged by size: 4366 1301 304 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2902 2934 3014 3046 3239 3271 2902 Size: 32 80 32 193 32 5602 Fragments arranged by size: 5602 193 80 32 32 32 AlwNI CAG_nnn'CTG Cuts at: 3714 5286 3714 Size: 1572 4399 ApaLI G'TGCA_C Cuts at: 2182 4028 5184 2182 Size: 1846 1156 2969 Fragments arranged by size: 2969 1846 1156 ApoI r'AATT_y Cuts at: 1493 2101 3280 3506 3658 5767 1493 Size: 608 1179 226 152 2109 1697 Fragments arranged by size: 2109 1697 1179 608 226 152 AseI AT'TA_AT Cuts at: 160 1032 4525 160 Size: 872 3493 1606 Fragments arranged by size: 3493 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5971 AvaI C'yCGr_G Cuts at: 1870 1943 2525 3296 1870 Size: 73 582 771 4545 Fragments arranged by size: 4545 771 582 73 BamHI G'GATC_C Cuts at: 3301 3668 3301 Size: 367 5604 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3292 4747 618 Size: 325 217 627 1505 1455 1842 Fragments arranged by size: 1842 1505 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3290 1391 Size: 1899 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5000 BbvCI CC'TCA_GC Cuts at: 3125 3125 Size: 5971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4162 4196 1903 Size: 34 2225 34 3678 Fragments arranged by size: 3678 2225 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3863 5073 3863 Size: 1210 4761 BclI T'GATC_A Cuts at: 1548 2155 1548 Size: 607 5364 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4582 136 Size: 107 122 71 631 605 2910 1525 Fragments arranged by size: 2910 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5966 5966 Size: 5971 BlpI GC'TnA_GC Cuts at: 3388 3388 Size: 5971 Bme1580I G_kGCm'C Cuts at: 1790 2186 3085 4032 5188 1790 Size: 396 899 947 1156 2573 Fragments arranged by size: 2573 1156 947 899 396 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5971 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2554 3702 4660 Size: 416 53 83 399 24 1137 1148 958 Cuts at: 4660 442 Size: 1753 Fragments arranged by size: 1753 1148 1137 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5971 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2661 2661 Size: 5971 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2688 3161 3167 4631 1239 Size: 1449 473 6 1464 2579 Fragments arranged by size: 2579 1464 1449 473 6 Bpu10I CC'TnA_GC Cuts at: 2073 3125 2073 Size: 1052 4919 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4096 4961 5259 4096 Size: 865 298 4808 Fragments arranged by size: 4808 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2522 4634 882 Size: 617 1023 2112 2219 Fragments arranged by size: 2219 2112 1023 617 BsaAI yAC'GTr Cuts at: 493 1204 2069 3086 3268 3762 493 Size: 711 865 1017 182 494 2702 Fragments arranged by size: 2702 1017 865 711 494 182 BsaBI GATnn'nnATC Cuts at: 5965 5965 Size: 5971 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3778 4160 4795 275 Size: 53 83 186 936 2245 382 635 1451 Fragments arranged by size: 2245 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2164 2626 4404 4802 5076 5223 5634 2164 Size: 462 1778 398 274 147 411 2501 Fragments arranged by size: 2501 1778 462 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2847 2877 2971 3001 3014 Size: 30 165 30 1444 30 94 30 13 Cuts at: 3014 3044 3239 3269 1178 Size: 30 195 30 3880 Fragments arranged by size: 3880 1444 195 165 94 30 30 30 30 30 30 13 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2025 3142 1186 Size: 839 1117 4015 Fragments arranged by size: 4015 1117 839 BseYI C'CCAG_C Cuts at: 2344 5174 5670 2344 Size: 2830 496 2645 Fragments arranged by size: 2830 2645 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2795 1163 Size: 573 1059 4339 Fragments arranged by size: 4339 1059 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3421 4182 5210 1540 Size: 275 186 1420 761 1028 2301 Fragments arranged by size: 2301 1420 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2186 2526 3134 4032 4117 5188 2186 Size: 340 608 898 85 1071 2969 Fragments arranged by size: 2969 1071 898 608 340 85 BsmI GAATG_Cn' Cuts at: 2892 3482 3575 2892 Size: 590 93 5288 Fragments arranged by size: 5288 590 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2186 2526 3085 3134 3290 4032 4117 Size: 399 396 340 559 49 156 742 85 Cuts at: 4117 5188 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 559 399 396 340 156 85 49 BspEI T'CCGG_A Cuts at: 2626 5634 2626 Size: 3008 2963 BspHI T'CATG_A Cuts at: 3860 5590 3860 Size: 1730 4241 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3326 844 Size: 2482 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3858 1394 Size: 429 2035 3507 Fragments arranged by size: 3507 2035 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4466 4640 65 Size: 1183 3218 174 1396 Fragments arranged by size: 3218 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3051 4615 4802 1700 Size: 110 1241 1564 187 2869 Fragments arranged by size: 2869 1564 1241 187 110 BsrGI T'GTAC_A Cuts at: 96 3063 96 Size: 2967 3004 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5971 BssSI C'ACGA_G Cuts at: 3074 4031 5043 3074 Size: 957 1012 4002 Fragments arranged by size: 4002 1012 957 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5971 BstBI TT'CG_AA Cuts at: 2007 4775 2007 Size: 2768 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5971 BstXI CCAn_nnnn'nTGG Cuts at: 2611 2611 Size: 5971 BstYI r'GATC_y Cuts at: 1972 2353 3301 3668 4053 4070 5522 5608 5620 Size: 381 948 367 385 17 1452 86 12 Cuts at: 5620 5966 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 948 385 381 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 3168 513 Size: 552 957 1146 3316 Fragments arranged by size: 3316 1146 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5232 Fragments arranged by size: 5232 683 56 BtsI GCAGTG_nn' Cuts at: 3487 4281 4301 3487 Size: 794 20 5157 Fragments arranged by size: 5157 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5971 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2642 2677 529 Size: 35 2078 35 3823 Fragments arranged by size: 3823 2078 35 35 DraI TTT'AAA Cuts at: 3275 3275 Size: 5971 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4978 809 Size: 507 3662 1802 Fragments arranged by size: 3662 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3418 4307 5936 Size: 54 1425 50 275 18 1588 889 1629 Cuts at: 5936 8 Size: 43 Fragments arranged by size: 1629 1588 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3418 1537 Size: 275 1606 4090 Fragments arranged by size: 4090 1606 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3901 1353 Size: 2548 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4558 4932 5078 387 Size: 1064 138 2969 374 146 1280 Fragments arranged by size: 2969 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2688 3161 3167 4034 4631 5418 5686 Size: 393 1056 473 6 867 597 787 268 Cuts at: 5686 1239 Size: 1524 Fragments arranged by size: 1524 1056 867 787 597 473 393 268 6 EcoO109I rG'GnC_Cy Cuts at: 2214 3220 2214 Size: 1006 4965 EcoRI G'AATT_C Cuts at: 3280 3658 3280 Size: 378 5593 FspI TGC'GCA Cuts at: 4477 4477 Size: 5971 HaeII r_GCGC'y Cuts at: 1678 5118 1678 Size: 3440 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2450 2482 2488 2520 2847 Size: 32 135 32 878 32 6 32 327 Cuts at: 2847 2879 4612 4644 4686 4718 1373 Size: 32 1733 32 42 32 2626 Fragments arranged by size: 2626 1733 878 327 135 42 32 32 32 32 32 32 32 6 HincII GTy'rAC Cuts at: 669 1657 1938 3241 3315 3561 669 Size: 988 281 1303 74 246 3079 Fragments arranged by size: 3079 1303 988 281 246 74 HindIII A'AGCT_T Cuts at: 748 748 Size: 5971 HpaI GTT'AAC Cuts at: 3561 3561 Size: 5971 KpnI G_GTAC'C Cuts at: 3296 3296 Size: 5971 MfeI C'AATT_G Cuts at: 3570 3570 Size: 5971 MluI A'CGCG_T Cuts at: 5847 5847 Size: 5971 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2403 5085 5269 1305 Size: 1098 2682 184 2007 Fragments arranged by size: 2682 2007 1098 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4149 Fragments arranged by size: 4149 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2609 2771 3929 4288 4447 518 Size: 648 1443 162 1158 359 159 2042 Fragments arranged by size: 2042 1443 1158 648 359 162 159 MspA1I CmG'CkG Cuts at: 1606 2324 2811 3387 4064 5212 5457 1606 Size: 718 487 576 677 1148 245 2120 Fragments arranged by size: 2120 1148 718 677 576 487 245 NaeI GCC'GGC Cuts at: 3053 3053 Size: 5971 NcoI C'CATG_G Cuts at: 513 1065 2022 3168 513 Size: 552 957 1146 3316 Fragments arranged by size: 3316 1146 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5971 NgoMIV G'CCGG_C Cuts at: 3051 3051 Size: 5971 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5971 NotI GC'GGCC_GC Cuts at: 3418 3418 Size: 5971 NspI r_CATG'y Cuts at: 1132 1229 3329 4874 1132 Size: 97 2100 1545 2229 Fragments arranged by size: 2229 2100 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5971 PciI A'CATG_T Cuts at: 4870 4870 Size: 5971 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5971 PfoI T'CCnGG_A Cuts at: 3033 3033 Size: 5971 PmeI GTTT'AAAC Cuts at: 3275 3275 Size: 5971 PmlI CAC'GTG Cuts at: 2069 3086 2069 Size: 1017 4954 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2663 2695 3014 3046 3239 3271 4040 4072 5553 Size: 32 319 32 193 32 769 32 1481 Cuts at: 5553 5585 2663 Size: 32 3049 Fragments arranged by size: 3049 1481 769 319 193 32 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2214 3220 2214 Size: 1006 4965 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5971 PsiI TTA'TAA Cuts at: 3541 3541 Size: 5971 PstI C_TGCA'G Cuts at: 830 2032 3323 830 Size: 1202 1291 3478 Fragments arranged by size: 3478 1291 1202 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5971 PvuII CAG'CTG Cuts at: 2324 2324 Size: 5971 SalI G'TCGA_C Cuts at: 1936 3313 1936 Size: 1377 4594 SbfI CC_TGCA'GG Cuts at: 3323 3323 Size: 5971 ScaI AGT'ACT Cuts at: 2543 4219 5931 2543 Size: 1676 1712 2583 Fragments arranged by size: 2583 1712 1676 SfcI C'TryA_G Cuts at: 826 1045 2028 3319 4454 5135 5326 826 Size: 219 983 1291 1135 681 191 1471 Fragments arranged by size: 1471 1291 1135 983 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5971 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5971 SmaI CCC'GGG Cuts at: 1872 3298 1872 Size: 1426 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2525 4075 4976 5238 820 Size: 197 926 582 1550 901 262 1553 Fragments arranged by size: 1553 1550 926 901 582 262 197 SnaBI TAC'GTA Cuts at: 493 3268 3762 493 Size: 2775 494 2702 Fragments arranged by size: 2775 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5971 SphI G_CATG'C Cuts at: 1132 3329 1132 Size: 2197 3774 SspI AAT'ATT Cuts at: 5 52 3895 5 Size: 47 3843 2081 Fragments arranged by size: 3843 2081 47 StuI AGG'CCT Cuts at: 1517 3159 1517 Size: 1642 4329 StyI C'CwwG_G Cuts at: 513 1065 2022 2210 2653 3168 3410 513 Size: 552 957 188 443 515 242 3074 Fragments arranged by size: 3074 957 552 515 443 242 188 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2509 3998 4015 4168 4353 4798 325 Size: 1255 929 1489 17 153 185 445 1498 Fragments arranged by size: 1498 1489 1255 929 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2541 3063 4217 Size: 274 80 33 51 1443 564 522 1154 Cuts at: 4217 5929 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1154 564 522 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5931 5963 5931 Size: 32 5939 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5971 XbaI T'CTAG_A Cuts at: 2719 3307 2719 Size: 588 5383 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3142 3142 Size: 5971 XhoI C'TCGA_G Cuts at: 1943 2525 1943 Size: 582 5389 XmaI C'CCGG_G Cuts at: 1870 3296 1870 Size: 1426 4545 XmnI GAAnn'nnTTC Cuts at: 4100 4100 Size: 5971 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3779 276 Size: 53 83 186 936 2245 2468 Fragments arranged by size: 2468 2245 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI