Restriction map for
pFN21ASDA0190
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# NO CUT ENZYMES ( 94 enzymes) #
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AccII AccIII AclI AflII AflIII
AgeI Alw44I Aor51HI ApaLI AscI
AvrII BbeI BbrPI BcgI BciVI
BfaI BfrI BglI BglII BlnI
BsaAI BsaBI BsaI BseAI BsiEI
BsiWI BsmBI BsmI BspEI BspLU11I
BsrBI BsrDI BssHII Bst1107I BstBI
BstEII BstPI BstUI BstZ17I CpoI
DraIII EagI EclXI Eco47III Eco52I
EcoNI EcoO65I EcoT22I FseI HaeII
HgaI KasI KspI MaeI MamI
MfeI MluI MroI MunI MvnI
NaeI NarI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PaeR7I PinAI PmaCI PmeI PmlI
PpuMI Psp1406I PvuI RsrII SacI
SacII SbfI ScaI SfiI SfoI
SfuI SmlI SnaBI SpeI SphI
Sse8387I SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 66 enzymes) #
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AatII 2272 GACGTC GACGT^C
Acc65I 838 GGTACC G^GTACC
AcyI 2272 GACGTC G[GA]^CG[CT]C
AhdI 799 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AocI 268 CCTCAGG CC^T[ACGT]AGG
AseI 1291 ATTAAT AT^TAAT
AsnI 1291 ATTAAT AT^TAAT
Asp718I 838 GGTACC G^GTACC
AspEI 799 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspHI 704 GAGCAC G[AT]GC[AT]^C
AspI 664 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2303 TGCGCA TGC^GCA
BalI 549 TGGCCA TGG^CCA
BamHI 1182 GGATCC G^GATCC
BbvCI 1585 GCTGAGG CCTCAGC(-5/-2)
BlpI 823 GCTCAGC GC^T[ACGT]AGC
Bpu1102I 823 GCTCAGC GC^T[ACGT]AGC
BsaHI 2272 GACGTC G[GA]^CG[CT]C
BsaWI 1170 TCCGGT [AT]^CCGG[AT]
BsiHKAI 704 GAGCAC G[AT]GC[AT]^C
BsmFI 568 GTCCC GGGAC(10/14)
Bsp1407I 295 TGTACA T^GTACA
BspDI 2308 ATCGAT AT^CGAT
BsrGI 295 TGTACA T^GTACA
BssSI 2059 CTCGTG CACGAG(-5/-1)
BstXI 911 CCAACGGGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 1182 GGATCC [GA]^GATC[CT]
Bsu36I 268 CCTCAGG CC^T[ACGT]AGG
CelII 823 GCTCAGC GC^T[ACGT]AGC
CfoI 2304 GCGC GCG^C
ClaI 2308 ATCGAT AT^CGAT
DraI 2191 TTTAAA TTT^AAA
DrdI 2314 GACCAGACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eam1105I 799 GACACCCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 268 CCTCAGG CC^T[ACGT]AGG
EcoRI 64 GAATTC G^AATTC
EcoRV 1915 GATATC GAT^ATC
FspI 2303 TGCGCA TGC^GCA
HhaI 2304 GCGC GCG^C
Hin1I 2272 GACGTC G[GA]^CG[CT]C
HinP1I 2304 GCGC G^CGC
HpaI 1543 GTTAAC GTT^AAC
KpnI 838 GGTACC GGTAC^C
MflI 1182 GGATCC [GA]^GATC[CT]
MluNI 549 TGGCCA TGG^CCA
MscI 549 TGGCCA TGG^CCA
MslI 1431 CACTAATATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 815 CAGCTG C[AC]G^C[GT]G
NcoI 14 CCATGG C^CATGG
NspI 611 ACATGC [GA]CATG^[CT]
PflFI 664 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 1779 GACTTCCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1291 ATTAAT AT^TAAT
PvuII 815 CAGCTG CAG^CTG
SalI 1571 GTCGAC G^TCGAC
SapI 1714 GAAGAGC GCTCTTC(1/4)
SexAI 2342 ACCAGGT A^CC[AT]GGT
SgrAI 1079 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 751 CCCGGG CCC^GGG
SspBI 295 TGTACA T^GTACA
SspI 2122 AATATT AAT^ATT
StuI 1375 AGGCCT AGG^CCT
Tth111I 664 GACTCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 1182 GGATCC [GA]^GATC[CT]
XmaCI 751 CCCGGG C^CCGGG
XmaI 751 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 21 enzymes) #
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AccI 1571 GTCGAC GT^[AC][GT]AC
2227 GTCTAC
AlwI 1183 GATCC GGATC(4/5)
1182 GGATC
ApaI 747 GGGCCC GGGCC^C
1764 GGGCCC
AvaII 951 GGACC G^G[AT]CC
2400 GGACC
BbsI 2091 GAAGAC GAAGAC(2/6)
2275 GTCTTC
BclI 1265 TGATCA T^GATCA
2330 TGATCA
BpuAI 2091 GAAGAC GAAGAC(2/6)
2275 GTCTTC
BseRI 86 CTCCTC GAGGAG(10/8)
1074 CTCCTC
BsgI 2065 CTGCAC GTGCAG(16/14)
584 GTGCAG
BsmAI 797 GAGAC GTCTC(1/5)
433 GTCTC
BspHI 1053 TCATGA T^CATGA
1620 TCATGA
BspMI 2402 ACCTGC ACCTGC(4/8)
1143 GCAGGT
BsrFI 1080 GCCGGT [GA]^CCGG[CT]
1822 ACCGGC
BstAPI 586 GCAGAGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
1309 GCATTGGTTGC
Cfr10I 1080 GCCGGT [GA]^CCGG[CT]
1822 ACCGGC
FbaI 1265 TGATCA T^GATCA
2330 TGATCA
HindIII 1645 AAGCTT A^AGCTT
2110 AAGCTT
PflMI 112 CCATACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1031 CCAAGTCCTGG
RcaI 1053 TCATGA T^CATGA
1620 TCATGA
Van91I 112 CCATACAGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1031 CCAAGTCCTGG
XcmI 530 CCACAGAAGCCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1118 CCATATCTCCCCTGG
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# 3 OR MORE CUT ENZYMES ( 77 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwNI
ApoI Asp700I AvaI BanI BanII
BbvI BcnI BmyI BpmI BsaJI
BsiYI BslI BsoBI Bsp1286I BsrI
BssKI BstF5I BstNI Cac8I Cfr13I
DdeI DpnII DraII DsaI EaeI
EarI EcoO109I EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HincII
HindII HinfI HpaII HphI ItaI
Ksp632I MaeII MaeIII MboI MboII
MnlI MseI MspI MvaI MwoI
NciI NdeII NlaIII NlaIV PleI
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XmnI