Restriction map for
pFN21ASDA0190
(Circular) (Six-base) MAPSORT of: pFN21ASDA0190.seq Check: 9813 from: 1 to: 7138
pFN21ASDA0190 7138 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4299 4948 278
Size: 53 83 186 936 2763 649 2468
Fragments arranged by size:
2763 2468 936 649 186 83 53
AccI GT'mk_AC
Cuts at: 1937 3595 4251 4481 1937
Size: 1658 656 230 4594
Fragments arranged by size:
4594 1658 656 230
Acc65I G'GTAC_C
Cuts at: 2861 4459 2861
Size: 1598 5540
AclI AA'CG_TT
Cuts at: 1563 5265 5638 1563
Size: 3702 373 3063
Fragments arranged by size:
3702 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2103 5201 6585 6853 1632
Size: 471 3098 1384 268 1917
Fragments arranged by size:
3098 1917 1384 471 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6941
AflIII A'CryG_T
Cuts at: 1203 6037 7014 1203
Size: 4834 977 1327
Fragments arranged by size:
4834 1327 977
AgeI A'CCGG_T
Cuts at: 5969 5969
Size: 7138
AhdI GACnn_n'nnGTC
Cuts at: 2827 5867 2827
Size: 3040 4098
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 7138
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 7138
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3788 3820 3788
Size: 32 7106
AlwNI CAG_nnn'CTG
Cuts at: 2396 2690 2813 4881 6453 2396
Size: 294 123 2068 1572 3081
Fragments arranged by size:
3081 2068 1572 294 123
ApaI G_GGCC'C
Cuts at: 2774 3791 2774
Size: 1017 6121
ApaLI G'TGCA_C
Cuts at: 5195 6351 5195
Size: 1156 5982
ApoI r'AATT_y
Cuts at: 1493 2087 2303 2621 4210 4447 4673 4825 6934
Size: 594 216 318 1589 237 226 152 2109
Cuts at: 6934 1493
Size: 1697
Fragments arranged by size:
2109 1697 1589 594 318 237 226 216
152
AseI AT'TA_AT
Cuts at: 160 1032 3315 5692 160
Size: 872 2283 2377 1606
Fragments arranged by size:
2377 2283 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7138
AvaI C'yCGr_G
Cuts at: 1870 1943 2774 2914 2987 4463 1870
Size: 73 831 140 73 1476 4545
Fragments arranged by size:
4545 1476 831 140 73 73
BamHI G'GATC_C
Cuts at: 3205 4468 4835 3205
Size: 1263 367 5508
Fragments arranged by size:
5508 1263 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2861 3032 4125 4459 5914
Size: 325 217 627 1074 171 1093 334 1455
Cuts at: 5914 618
Size: 1842
Fragments arranged by size:
1842 1455 1093 1074 627 334 325 217
171
BanII G_rGCy'C
Cuts at: 1391 2774 2848 3023 3540 3791 4457 1391
Size: 1383 74 175 517 251 666 4072
Fragments arranged by size:
4072 1383 666 517 251 175 74
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4121 4291 928
Size: 971 2222 170 3775
Fragments arranged by size:
3775 2222 971 170
BbvCI CC'TCA_GC
Cuts at: 3609 3609
Size: 7138
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5329 5363 1903
Size: 34 3392 34 3678
Fragments arranged by size:
3678 3392 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5030 6240 5030
Size: 1210 5928
BclI T'GATC_A
Cuts at: 1548 3288 4353 1548
Size: 1740 1065 4333
Fragments arranged by size:
4333 1740 1065
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5749 136
Size: 107 122 71 631 605 4077 1525
Fragments arranged by size:
4077 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 7133 7133
Size: 7138
BlpI GC'TnA_GC
Cuts at: 2847 4555 2847
Size: 1708 5430
Bme1580I G_kGCm'C
Cuts at: 1790 2774 2927 3791 5199 6355 1790
Size: 984 153 864 1408 1156 2573
Fragments arranged by size:
2573 1408 1156 984 864 153
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 7138
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2232 4869 5827
Size: 416 53 83 399 24 815 2637 958
Cuts at: 5827 442
Size: 1753
Fragments arranged by size:
2637 1753 958 815 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7138
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3257 3543 3998 4176 5798 1239
Size: 2018 286 455 178 1622 2579
Fragments arranged by size:
2579 2018 1622 455 286 178
Bpu10I CC'TnA_GC
Cuts at: 3609 3609
Size: 7138
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 5263 6128 6426 5263
Size: 865 298 5975
Fragments arranged by size:
5975 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5801 882
Size: 617 4302 2219
Fragments arranged by size:
4302 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4435 4929 493
Size: 711 3231 494 2702
Fragments arranged by size:
3231 2702 711 494
BsaBI GATnn'nnATC
Cuts at: 7132 7132
Size: 7138
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4296 4945 5327 5962
Size: 53 83 186 936 2763 649 382 635
Cuts at: 5962 275
Size: 1451
Fragments arranged by size:
2763 1451 936 649 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 3193 5571 5969 6243 6390 6801 3193
Size: 2378 398 274 147 411 3530
Fragments arranged by size:
3530 2378 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6913
Fragments arranged by size:
6913 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2100 3088 1186
Size: 914 988 5236
Fragments arranged by size:
5236 988 914
BseYI C'CCAG_C
Cuts at: 2261 2840 6341 6837 2261
Size: 579 3501 496 2562
Fragments arranged by size:
3501 2562 579 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2628 4073 1163
Size: 573 892 1445 4228
Fragments arranged by size:
4228 1445 892 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4588 5349 6377 1540
Size: 275 186 2587 761 1028 2301
Fragments arranged by size:
2587 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2731 5199 5284 6355 2731
Size: 2468 85 1071 3514
Fragments arranged by size:
3514 2468 1071 85
BsmI GAATG_Cn'
Cuts at: 4649 4742 4649
Size: 93 7045
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2731 2774 2848 2927 3023 3540 3791
Size: 399 941 43 74 79 96 517 251
Cuts at: 3791 4457 5199 5284 6355 1391
Size: 666 742 85 1071 2174
Fragments arranged by size:
2174 1071 941 742 666 517 399 251
96 85 79 74 43
BspEI T'CCGG_A
Cuts at: 6801 6801
Size: 7138
BspHI T'CATG_A
Cuts at: 3076 3643 5027 6757 3076
Size: 567 1384 1730 3457
Fragments arranged by size:
3457 1730 1384 567
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 3157 4434 4493 844
Size: 2313 1277 59 3489
Fragments arranged by size:
3489 2313 1277 59
BsrBI CCG'CTC
Cuts at: 1394 1823 5025 1394
Size: 429 3202 3507
Fragments arranged by size:
3507 3202 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 5633 5807 65
Size: 1183 4385 174 1396
Fragments arranged by size:
4385 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 3103 3845 5782 5969 1700
Size: 110 1293 742 1937 187 2869
Fragments arranged by size:
2869 1937 1293 742 187 110
BsrGI T'GTAC_A
Cuts at: 96 2318 96
Size: 2222 4916
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7138
BssSI C'ACGA_G
Cuts at: 4082 5198 6210 4082
Size: 1116 1012 5010
Fragments arranged by size:
5010 1116 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2615 3338 794
Size: 1821 723 4594
Fragments arranged by size:
4594 1821 723
BstBI TT'CG_AA
Cuts at: 2007 5942 2007
Size: 3935 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 7138
BstXI CCAn_nnnn'nTGG
Cuts at: 2941 2941
Size: 7138
BstYI r'GATC_y
Cuts at: 1972 3205 4468 4835 5220 5237 6689 6775 6787
Size: 1233 1263 367 385 17 1452 86 12
Cuts at: 6787 7133 1972
Size: 346 1977
Fragments arranged by size:
1977 1452 1263 1233 385 367 346 86
17 12
Bsu36I CC'TnA_GG
Cuts at: 2292 2292
Size: 7138
BtgI C'CryG_G
Cuts at: 513 1065 2037 2955 3280 513
Size: 552 972 918 325 4371
Fragments arranged by size:
4371 972 918 552 325
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2553 487
Size: 683 56 1327 5072
Fragments arranged by size:
5072 1327 683 56
BtsI GCAGTG_nn'
Cuts at: 2743 3323 4251 4654 5448 5468 2743
Size: 580 928 403 794 20 4413
Fragments arranged by size:
4413 928 794 580 403 20
ClaI AT'CG_AT
Cuts at: 1554 4332 1554
Size: 2778 4360
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 7103
DraI TTT'AAA
Cuts at: 4216 4442 4216
Size: 226 6912
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4343 6145 809
Size: 507 3027 1802 1802
Fragments arranged by size:
3027 1802 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2501 2572 4279
Size: 54 1425 50 275 18 671 71 1707
Cuts at: 4279 4585 5474 7103 8
Size: 306 889 1629 43
Fragments arranged by size:
1707 1629 1425 889 671 306 275 71
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 4585 1537
Size: 275 2773 4090
Fragments arranged by size:
4090 2773 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3097 3732 4045 5068 1353
Size: 1744 635 313 1023 3423
Fragments arranged by size:
3423 1744 1023 635 313
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2447 3836 5725 6099 6245 387
Size: 1064 138 858 1389 1889 374 146 1280
Fragments arranged by size:
1889 1389 1280 1064 858 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2103 3257 3543 3998 4176 5201 5798
Size: 393 471 1154 286 455 178 1025 597
Cuts at: 5798 6585 6853 1239
Size: 787 268 1524
Fragments arranged by size:
1524 1154 1025 787 597 471 455 393
286 268 178
EcoO109I rG'GnC_Cy
Cuts at: 2296 2770 2771 3788 2296
Size: 474 1 1017 5646
Fragments arranged by size:
5646 1017 474 1
EcoRI G'AATT_C
Cuts at: 2087 4447 4825 2087
Size: 2360 378 4400
Fragments arranged by size:
4400 2360 378
EcoRV GAT'ATC
Cuts at: 3940 3940
Size: 7138
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3203 4202 3203
Size: 999 6139
FspI TGC'GCA
Cuts at: 4328 5644 4328
Size: 1316 5822
HaeII r_GCGC'y
Cuts at: 1678 6285 1678
Size: 4607 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2441 2473 2534 2566 2813
Size: 32 135 32 869 32 61 32 247
Cuts at: 2813 2845 2981 3013 3486 3518 3929 3961 3987
Size: 32 136 32 473 32 411 32 26
Cuts at: 3987 4019 5779 5811 5853 5885 1373
Size: 32 1760 32 42 32 2626
Fragments arranged by size:
2626 1760 869 473 411 247 136 135
61 42 32 32 32 32 32 32
32 32 32 32 32 26
HincII GTy'rAC
Cuts at: 669 1657 1938 3411 3568 3596 4482 4728 669
Size: 988 281 1473 157 28 886 246 3079
Fragments arranged by size:
3079 1473 988 886 281 246 157 28
HindIII A'AGCT_T
Cuts at: 748 3668 4133 748
Size: 2920 465 3753
Fragments arranged by size:
3753 2920 465
HpaI GTT'AAC
Cuts at: 3568 4728 3568
Size: 1160 5978
KpnI G_GTAC'C
Cuts at: 2865 4463 2865
Size: 1598 5540
MfeI C'AATT_G
Cuts at: 4737 4737
Size: 7138
MluI A'CGCG_T
Cuts at: 7014 7014
Size: 7138
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3729 3915 6252 6436 1305
Size: 2424 186 2337 184 2007
Fragments arranged by size:
2424 2337 2007 186 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2574 10
Size: 54 1425 343 742 4574
Fragments arranged by size:
4574 1425 742 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3458 5096 5455 5614 518
Size: 648 2292 1638 359 159 2042
Fragments arranged by size:
2292 2042 1638 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2840 4554 5231 6379 6624 1606
Size: 1234 1714 677 1148 245 2120
Fragments arranged by size:
2120 1714 1234 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2037 513
Size: 552 972 5614
Fragments arranged by size:
5614 972 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7138
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7138
NotI GC'GGCC_GC
Cuts at: 4585 4585
Size: 7138
NspI r_CATG'y
Cuts at: 1132 1229 2638 4496 6041 1132
Size: 97 1409 1858 1545 2229
Fragments arranged by size:
2229 1858 1545 1409 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 7138
PciI A'CATG_T
Cuts at: 6037 6037
Size: 7138
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2141 3060 1108
Size: 1033 919 5186
Fragments arranged by size:
5186 1033 919
PfoI T'CCnGG_A
Cuts at: 3966 3966
Size: 7138
PmeI GTTT'AAAC
Cuts at: 4442 4442
Size: 7138
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5207 5239 6720 6752 5207
Size: 32 1481 32 5593
Fragments arranged by size:
5593 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 3806 1613
Size: 2193 4945
PsiI TTA'TAA
Cuts at: 4708 4708
Size: 7138
PspOMI G'GGCC_C
Cuts at: 2770 3787 2770
Size: 1017 6121
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 3293 3325 3293
Size: 32 7106
PstI C_TGCA'G
Cuts at: 830 2755 2809 3330 4490 830
Size: 1925 54 521 1160 3478
Fragments arranged by size:
3478 1925 1160 521 54
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7138
PvuII CAG'CTG
Cuts at: 2840 2840
Size: 7138
SalI G'TCGA_C
Cuts at: 1936 3594 4480 1936
Size: 1658 886 4594
Fragments arranged by size:
4594 1658 886
SapI GCTCTTCn'nnn_
Cuts at: 3732 3732
Size: 7138
SbfI CC_TGCA'GG
Cuts at: 4490 4490
Size: 7138
ScaI AGT'ACT
Cuts at: 5386 7098 5386
Size: 1712 5426
SexAI A'CCwGG_T
Cuts at: 4365 4365
Size: 7138
SfcI C'TryA_G
Cuts at: 826 1045 2266 2751 2805 3210 3326 4290 4486
Size: 219 1221 485 54 405 116 964 196
Cuts at: 4486 5621 6302 6493 826
Size: 1135 681 191 1471
Fragments arranged by size:
1471 1221 1135 964 681 485 405 219
196 191 116 54
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7138
SgrAI Cr'CCGG_yG
Cuts at: 1700 3103 1700
Size: 1403 5735
SmaI CCC'GGG
Cuts at: 1872 2776 4465 1872
Size: 904 1689 4545
Fragments arranged by size:
4545 1689 904
SmlI C'TyrA_G
Cuts at: 820 1017 1943 5242 6143 6405 820
Size: 197 926 3299 901 262 1553
Fragments arranged by size:
3299 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4435 4929 493
Size: 3942 494 2702
Fragments arranged by size:
3942 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7138
SphI G_CATG'C
Cuts at: 1132 4496 1132
Size: 3364 3774
SspI AAT'ATT
Cuts at: 5 52 4147 5062 5
Size: 47 4095 915 2081
Fragments arranged by size:
4095 2081 915 47
StuI AGG'CCT
Cuts at: 1517 3400 1517
Size: 1883 5255
StyI C'CwwG_G
Cuts at: 513 1065 2037 2881 4006 4577 513
Size: 552 972 844 1125 571 3074
Fragments arranged by size:
3074 1125 972 844 571 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2618 3430 3715 4113 4298 5165 5182
Size: 1255 1038 812 285 398 185 867 17
Cuts at: 5182 5335 5520 5965 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1255 1038 867 812 445 398 285
185 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2318 5384 7096
Size: 274 80 33 51 1443 341 3066 1712
Cuts at: 7096 96
Size: 138
Fragments arranged by size:
3066 1712 1443 341 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 7098 7130 7098
Size: 32 7106
Tth111I GACn'n_nGTC
Cuts at: 1316 2690 1316
Size: 1374 5764
XbaI T'CTAG_A
Cuts at: 4474 4474
Size: 7138
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2560 3148 2560
Size: 588 6550
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 7138
XmaI C'CCGG_G
Cuts at: 1870 2774 4463 1870
Size: 904 1689 4545
Fragments arranged by size:
4545 1689 904
XmnI GAAnn'nnTTC
Cuts at: 2307 3449 4100 5267 2307
Size: 1142 651 1167 4178
Fragments arranged by size:
4178 1167 1142 651
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4297 4946 276
Size: 53 83 186 936 2763 649 2468
Fragments arranged by size:
2763 2468 936 649 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI
BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI
BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI
BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI
Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI
EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV
FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI
MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI
NheI NotI NspI PasI PciI PflMI PfoI PmeI
PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII
SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI
SmaI SmlI SnaBI SpeI SphI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI AvrII BaeI BbeI BfrBI BplI
BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI FseI FspAI
KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI
PpuMI PspXI RsrII SacI SacII SanDI SfiI SfoI
SrfI SwaI