Restriction map for
pFN21ASDA0190
(Linear) (Six-base) MAPSORT of: KIAA0190.seq Check: 9286 from: 1 to: 2409
KIAA0190.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AatII G_ACGT'C
Cuts at: 0 2276 2409
Size: 2276 133
AccI GT'mk_AC
Cuts at: 0 1572 2228 2409
Size: 1572 656 181
Fragments arranged by size:
1572 656 181
Acc65I G'GTAC_C
Cuts at: 0 838 2409
Size: 838 1571
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 2409
Size: 80 2329
AhdI GACnn_n'nnGTC
Cuts at: 0 804 2409
Size: 804 1605
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1765 1797 2409
Size: 1765 32 612
Fragments arranged by size:
1765 612 32
AlwNI CAG_nnn'CTG
Cuts at: 0 373 667 790 2409
Size: 373 294 123 1619
Fragments arranged by size:
1619 373 294 123
ApaI G_GGCC'C
Cuts at: 0 751 1768 2409
Size: 751 1017 641
Fragments arranged by size:
1017 751 641
ApoI r'AATT_y
Cuts at: 0 64 280 598 2187 2409
Size: 64 216 318 1589 222
Fragments arranged by size:
1589 318 222 216 64
AseI AT'TA_AT
Cuts at: 0 1292 2409
Size: 1292 1117
AvaI C'yCGr_G
Cuts at: 0 751 891 964 2409
Size: 751 140 73 1445
Fragments arranged by size:
1445 751 140 73
BamHI G'GATC_C
Cuts at: 0 1182 2409
Size: 1182 1227
BanI G'GyrC_C
Cuts at: 0 838 1009 2102 2409
Size: 838 171 1093 307
Fragments arranged by size:
1093 838 307 171
BanII G_rGCy'C
Cuts at: 0 751 825 1000 1517 1768 2409
Size: 751 74 175 517 251 641
Fragments arranged by size:
751 641 517 251 175 74
BbsI GAAGACnn'nnnn_
Cuts at: 0 2098 2268 2409
Size: 2098 170 141
Fragments arranged by size:
2098 170 141
BbvCI CC'TCA_GC
Cuts at: 0 1586 2409
Size: 1586 823
BclI T'GATC_A
Cuts at: 0 1265 2330 2409
Size: 1265 1065 79
Fragments arranged by size:
1265 1065 79
BlpI GC'TnA_GC
Cuts at: 0 824 2409
Size: 824 1585
Bme1580I G_kGCm'C
Cuts at: 0 751 904 1768 2409
Size: 751 153 864 641
Fragments arranged by size:
864 751 641 153
BmrI ACTGGGnnnn_n'
Cuts at: 0 209 2409
Size: 209 2200
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1234 1520 1975 2153 2409
Size: 1234 286 455 178 256
Fragments arranged by size:
1234 455 286 256 178
Bpu10I CC'TnA_GC
Cuts at: 0 1586 2409
Size: 1586 823
BsaHI Gr'CG_yC
Cuts at: 0 2273 2409
Size: 2273 136
BsaWI w'CCGG_w
Cuts at: 0 1170 2409
Size: 1170 1239
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 77 1065 2409
Size: 77 988 1344
Fragments arranged by size:
1344 988 77
BseYI C'CCAG_C
Cuts at: 0 238 817 2409
Size: 238 579 1592
Fragments arranged by size:
1592 579 238
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 605 2050 2409
Size: 605 1445 359
Fragments arranged by size:
1445 605 359
BsiHKAI G_wGCw'C
Cuts at: 0 708 2409
Size: 708 1701
Bsp1286I G_dGCh'C
Cuts at: 0 708 751 825 904 1000 1517 1768 2409
Size: 708 43 74 79 96 517 251 641
Fragments arranged by size:
708 641 517 251 96 79 74 43
BspHI T'CATG_A
Cuts at: 0 1053 1620 2409
Size: 1053 567 789
Fragments arranged by size:
1053 789 567
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 1134 2409
Size: 1134 1275
BsrFI r'CCGG_y
Cuts at: 0 1080 1822 2409
Size: 1080 742 587
Fragments arranged by size:
1080 742 587
BsrGI T'GTAC_A
Cuts at: 0 295 2409
Size: 295 2114
BssSI C'ACGA_G
Cuts at: 0 2059 2409
Size: 2059 350
BstAPI GCAn_nnn'nTGC
Cuts at: 0 592 1315 2409
Size: 592 723 1094
Fragments arranged by size:
1094 723 592
BstXI CCAn_nnnn'nTGG
Cuts at: 0 918 2409
Size: 918 1491
BstYI r'GATC_y
Cuts at: 0 1182 2409
Size: 1182 1227
Bsu36I CC'TnA_GG
Cuts at: 0 269 2409
Size: 269 2140
BtgI C'CryG_G
Cuts at: 0 14 932 1257 2409
Size: 14 918 325 1152
Fragments arranged by size:
1152 918 325 14
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 530 2409
Size: 530 1879
BtsI GCAGTG_nn'
Cuts at: 0 720 1300 2228 2409
Size: 720 580 928 181
Fragments arranged by size:
928 720 580 181
ClaI AT'CG_AT
Cuts at: 0 2309 2409
Size: 2309 100
DraI TTT'AAA
Cuts at: 0 2193 2409
Size: 2193 216
DrdI GACnn_nn'nnGTC
Cuts at: 0 2320 2409
Size: 2320 89
EaeI y'GGCC_r
Cuts at: 0 478 549 2256 2409
Size: 478 71 1707 153
Fragments arranged by size:
1707 478 153 71
EarI CTCTTCn'nnn_
Cuts at: 0 1074 1709 2022 2409
Size: 1074 635 313 387
Fragments arranged by size:
1074 635 387 313
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 424 1813 2409
Size: 424 1389 596
Fragments arranged by size:
1389 596 424
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 80 1234 1520 1975 2153 2409
Size: 80 1154 286 455 178 256
Fragments arranged by size:
1154 455 286 256 178 80
EcoO109I rG'GnC_Cy
Cuts at: 0 273 747 748 1765 2409
Size: 273 474 1 1017 644
Fragments arranged by size:
1017 644 474 273 1
EcoRI G'AATT_C
Cuts at: 0 64 2409
Size: 64 2345
EcoRV GAT'ATC
Cuts at: 0 1917 2409
Size: 1917 492
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1180 2179 2409
Size: 1180 999 230
Fragments arranged by size:
1180 999 230
FspI TGC'GCA
Cuts at: 0 2305 2409
Size: 2305 104
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 418 450 511 543 790 822 958 990
Size: 418 32 61 32 247 32 136 32
Cuts at: 990 1463 1495 1906 1938 1964 1996 2409
Size: 473 32 411 32 26 32 413
Fragments arranged by size:
473 418 413 411 247 136 61 32
32 32 32 32 32 32 26
HincII GTy'rAC
Cuts at: 0 1388 1545 1573 2409
Size: 1388 157 28 836
Fragments arranged by size:
1388 836 157 28
HindIII A'AGCT_T
Cuts at: 0 1645 2110 2409
Size: 1645 465 299
Fragments arranged by size:
1645 465 299
HpaI GTT'AAC
Cuts at: 0 1545 2409
Size: 1545 864
KpnI G_GTAC'C
Cuts at: 0 842 2409
Size: 842 1567
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1706 1892 2409
Size: 1706 186 517
Fragments arranged by size:
1706 517 186
MscI TGG'CCA
Cuts at: 0 551 2409
Size: 551 1858
MslI CAynn'nnrTG
Cuts at: 0 1435 2409
Size: 1435 974
MspA1I CmG'CkG
Cuts at: 0 817 2409
Size: 817 1592
NcoI C'CATG_G
Cuts at: 0 14 2409
Size: 14 2395
NspI r_CATG'y
Cuts at: 0 615 2409
Size: 615 1794
PflMI CCAn_nnn'nTGG
Cuts at: 0 118 1037 2409
Size: 118 919 1372
Fragments arranged by size:
1372 919 118
PfoI T'CCnGG_A
Cuts at: 0 1943 2409
Size: 1943 466
PshAI GACnn'nnGTC
Cuts at: 0 1783 2409
Size: 1783 626
PspOMI G'GGCC_C
Cuts at: 0 747 1764 2409
Size: 747 1017 645
Fragments arranged by size:
1017 747 645
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1270 1302 2409
Size: 1270 32 1107
Fragments arranged by size:
1270 1107 32
PstI C_TGCA'G
Cuts at: 0 732 786 1307 2409
Size: 732 54 521 1102
Fragments arranged by size:
1102 732 521 54
PvuII CAG'CTG
Cuts at: 0 817 2409
Size: 817 1592
SalI G'TCGA_C
Cuts at: 0 1571 2409
Size: 1571 838
SapI GCTCTTCn'nnn_
Cuts at: 0 1709 2409
Size: 1709 700
SexAI A'CCwGG_T
Cuts at: 0 2342 2409
Size: 2342 67
SfcI C'TryA_G
Cuts at: 0 243 728 782 1187 1303 2267 2409
Size: 243 485 54 405 116 964 142
Fragments arranged by size:
964 485 405 243 142 116 54
SgrAI Cr'CCGG_yG
Cuts at: 0 1080 2409
Size: 1080 1329
SmaI CCC'GGG
Cuts at: 0 753 2409
Size: 753 1656
SspI AAT'ATT
Cuts at: 0 2124 2409
Size: 2124 285
StuI AGG'CCT
Cuts at: 0 1377 2409
Size: 1377 1032
StyI C'CwwG_G
Cuts at: 0 14 858 1983 2409
Size: 14 844 1125 426
Fragments arranged by size:
1125 844 426 14
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 595 1407 1692 2090 2275 2409
Size: 595 812 285 398 185 134
Fragments arranged by size:
812 595 398 285 185 134
TatI w'GTAC_w
Cuts at: 0 295 2409
Size: 295 2114
Tth111I GACn'n_nGTC
Cuts at: 0 667 2409
Size: 667 1742
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 537 1125 2409
Size: 537 588 1284
Fragments arranged by size:
1284 588 537
XmaI C'CCGG_G
Cuts at: 0 751 2409
Size: 751 1658
XmnI GAAnn'nnTTC
Cuts at: 0 284 1426 2077 2409
Size: 284 1142 651 332
Fragments arranged by size:
1142 651 332 284
ZraI GAC'GTC
Cuts at: 0 2274 2409
Size: 2274 135
Enzymes that do cut:
AatII AccI Acc65I AcuI AhdI AloI AlwNI ApaI
ApoI AseI AvaI BamHI BanI BanII BbsI BbvCI
BclI BlpI Bme1580I BmrI BpmI Bpu10I BsaHI BsaWI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspHI BspMI BsrFI
BsrGI BssSI BstAPI BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI DraI DrdI EaeI EarI EciI Eco57MI
EcoO109I EcoRI EcoRV FalI FspI Hin4I HincII HindIII
HpaI KpnI MmeI MscI MslI MspA1I NcoI NspI
PflMI PfoI PshAI PspOMI PsrI PstI PvuII SalI
SapI SexAI SfcI SgrAI SmaI SspI StuI StyI
TaqII TatI Tth111I XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AclI AfeI AflII AflIII AgeI AleI AlfI
ApaLI AscI AsiSI AvrII BaeI BbeI BcgI BciVI
BfrBI BglI BglII BmgBI BmtI BplI BpuEI BsaI
BsaAI BsaBI BsaXI BsiEI BsiWI BsmI BsmBI BspEI
BsrBI BsrDI BssHII BstBI BstEII BstZ17I CspCI DraIII
EagI EcoICRI EcoNI FseI FspAI HaeII KasI MfeI
MluI NaeI NarI NdeI NgoMIV NheI NotI NruI
NsiI PacI PasI PciI PmeI PmlI PpiI PpuMI
PsiI PspXI PvuI RsrII SacI SacII SanDI SbfI
ScaI SfiI SfoI SgfI SmlI SnaBI SpeI SphI
SrfI SwaI TstI XbaI XhoI