Restriction map for
pFN21ASDA0173
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# NO CUT ENZYMES ( 93 enzymes) #
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AatII Acc65I AccII AflII AflIII
AgeI AhdI Aor51HI AscI AseI
AsnI Asp718I AspEI AvrII BbeI
BbrPI BcgI BciVI BfrI BlnI
BlpI Bpu1102I BsaBI BsiEI BsiWI
BsmBI Bsp1407I BspDI BspLU11I BsrGI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstZ17I CelII ClaI CpoI
DraI DraIII EagI Eam1105I EclXI
Eco47III Eco52I EcoRI FseI HaeII
HpaI KasI KpnI KspI MamI
MfeI MluI MunI MvnI NaeI
NarI NdeI NgoMIV NotI NruI
NspV PacI PinAI PmaCI PmeI
PmlI PshBI PvuI RsrII SacI
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SnaBI SphI Sse8387I SspBI SspI
SwaI XmaCI XmaI
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# ONE CUT ENZYMES ( 48 enzymes) #
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AccI 861 GTCTAC GT^[AC][GT]AC
AccIII 3200 TCCGGA T^CCGGA
AclI 1744 AACGTT AA^CGTT
Alw44I 782 GTGCAC G^TGCAC
ApaI 201 GGGCCC GGGCC^C
ApaLI 782 GTGCAC G^TGCAC
Asp700I 2061 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspI 183 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AviII 2117 TGCGCA TGC^GCA
BamHI 984 GGATCC G^GATCC
BcnI 324 CCCGG CC^[GC]GG
BglII 3192 AGATCT A^GATCT
BsaAI 2036 CACGTA [CT]AC^GT[GA]
BseAI 3200 TCCGGA T^CCGGA
BsgI 2924 CTGCAC GTGCAG(16/14)
BspEI 3200 TCCGGA T^CCGGA
BspHI 1698 TCATGA T^CATGA
BsrBI 357 CCGCTC CCGCTC(-3/-3)
BsrDI 285 CATTGC GCAATG(2/0)
BsrFI 2015 ACCGGC [GA]^CCGG[CT]
BstEII 1924 GGTCACC G^GT[ACGT]ACC
BstPI 1924 GGTCACC G^GT[ACGT]ACC
CfoI 2118 GCGC GCG^C
Cfr10I 2015 ACCGGC [GA]^CCGG[CT]
DrdI 1228 GACAATACAGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoNI 2094 CCTGCCCCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 1924 GGTCACC G^GT[ACGT]ACC
EcoT22I 2984 ATGCAT ATGCA^T
FspI 2117 TGCGCA TGC^GCA
HhaI 2118 GCGC GCG^C
HinP1I 2118 GCGC G^CGC
HindIII 3178 AAGCTT A^AGCTT
MroI 3200 TCCGGA T^CCGGA
NciI 324 CCCGG CC^[GC]GG
NheI 868 GCTAGC G^CTAGC
NsiI 2984 ATGCAT ATGCA^T
PaeR7I 2177 CTCGAG C^TCGAG
PflFI 183 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PshAI 2893 GACAAATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 1744 AACGTT AA^CGTT
PstI 2853 CTGCAG CTGCA^G
RcaI 1698 TCATGA T^CATGA
SpeI 1542 ACTAGT A^CTAGT
StuI 207 AGGCCT AGG^CCT
Tth111I 183 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 1657 TCTAGA T^CTAGA
XhoI 2177 CTCGAG C^TCGAG
XmnI 2061 GAAGGAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 19 enzymes) #
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AvaI 1772 CTCGGG C^[CT]CG[GA]G
2177 CTCGAG
BanI 807 GGTGCC G^G[CT][GA]CC
1204 GGTGCC
BbvCI 5 CCTCAGC CCTCAGC(-5/-2)
1328 CCTCAGC
BclI 2970 TGATCA T^GATCA
3512 TGATCA
BsaI 1440 GAGACC GGTCTC(1/5)
3260 GGTCTC
BsaWI 917 ACCGGA [AT]^CCGG[AT]
3200 TCCGGA
BsmI 2448 GAATGC GAATGC(1/-1)
1259 GCATTC
BsoBI 1772 CTCGGG C^[CT]CG[GA]G
2177 CTCGAG
BstXI 1412 CCATCCAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1454 CCAGGGAGCTGG
EcoRV 2758 GATATC GAT^ATC
2830 GATATC
FauI 1400 CCCGC CCCGC(4/6)
1191 GCGGG
FbaI 2970 TGATCA T^GATCA
3512 TGATCA
HincII 796 GTTGAC GT[CT]^[GA]AC
2719 GTCAAC
HindII 796 GTTGAC GT[CT]^[GA]AC
2719 GTCAAC
MseI 1618 TTAA T^TAA
3182 TTAA
MslI 681 CACGCCAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1279 CATGCCAGTG
NspI 1278 ACATGC [GA]CATG^[CT]
2916 GCATGT
SapI 582 GAAGAGC GCTCTTC(1/4)
2394 GAAGAGC
Tru9I 1618 TTAA T^TAA
3182 TTAA
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# 3 OR MORE CUT ENZYMES ( 98 enzymes) #
#########################################
AciI AcsI AcyI AfaI AluI
AlwI AlwNI AocI ApoI AspHI
AvaII BalI BanII BbsI BbvI
BfaI BglI BmyI BpmI BpuAI
BsaHI BsaJI BseRI BsiHKAI BsiYI
BslI BsmAI BsmFI Bsp1286I BspMI
BsrI BssKI BstF5I BstNI BstYI
Bsu36I Cac8I Cfr13I DdeI DpnII
DraII DsaI EaeI EarI Eco81I
EcoO109I EcoRII EcoT14I Fnu4HI FokI
HaeIII HapII HgaI Hin1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MflI
MluNI MnlI MscI MspA1I MspI
MvaI MwoI NcoI NdeII NlaIII
NlaIV PflMI PleI PpuMI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
TseI Tsp45I Tsp509I TspRI TthHB8I
Van91I XcmI XhoII