(Circular) (Six-base) MAPSORT of: pFN21ASDA0173.seq Check: 3859 from: 1 to: 8326 pFN21ASDA0173 8326 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6136 278 Size: 53 83 186 936 4600 2468 Fragments arranged by size: 4600 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2885 5669 1937 Size: 948 2784 4594 Fragments arranged by size: 4594 2784 948 Acc65I G'GTAC_C Cuts at: 5647 5647 Size: 8326 AclI AA'CG_TT Cuts at: 1563 3768 6453 6826 1563 Size: 2205 2685 373 3063 Fragments arranged by size: 3063 2685 2205 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2583 2759 3567 4550 5323 5424 6389 7773 Size: 951 176 808 983 773 101 965 1384 Cuts at: 7773 8041 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 983 965 951 808 773 268 176 101 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8129 AflIII A'CryG_T Cuts at: 1203 7225 8202 1203 Size: 6022 977 1327 Fragments arranged by size: 6022 1327 977 AgeI A'CCGG_T Cuts at: 7157 7157 Size: 8326 AhdI GACnn_n'nnGTC Cuts at: 7055 7055 Size: 8326 AleI CACnn'nnGTG Cuts at: 1166 2708 1166 Size: 1542 6784 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2726 4962 1184 Size: 1542 2236 4548 Fragments arranged by size: 4548 2236 1542 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3011 3043 3011 Size: 32 8294 AlwNI CAG_nnn'CTG Cuts at: 2108 2145 2328 2413 2585 2737 3518 4875 6069 Size: 37 183 85 172 152 781 1357 1194 Cuts at: 6069 7641 2108 Size: 1572 2793 Fragments arranged by size: 2793 1572 1357 1194 781 183 172 152 85 37 ApaI G_GGCC'C Cuts at: 2228 2228 Size: 8326 ApaLI G'TGCA_C Cuts at: 2805 6383 7539 2805 Size: 3578 1156 3592 Fragments arranged by size: 3592 3578 1156 ApoI r'AATT_y Cuts at: 1493 3696 4986 5097 5635 5861 6013 8122 1493 Size: 2203 1290 111 538 226 152 2109 1697 Fragments arranged by size: 2203 2109 1697 1290 538 226 152 111 AseI AT'TA_AT Cuts at: 160 1032 6880 160 Size: 872 5848 1606 Fragments arranged by size: 5848 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8326 AvaI C'yCGr_G Cuts at: 1870 1943 3795 4200 5651 1870 Size: 73 1852 405 1451 4545 Fragments arranged by size: 4545 1852 1451 405 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 4256 4289 4256 Size: 33 8293 BamHI G'GATC_C Cuts at: 3007 5656 6023 3007 Size: 2649 367 5310 Fragments arranged by size: 5310 2649 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2830 3227 5647 7102 618 Size: 325 217 627 1043 397 2420 1455 1842 Fragments arranged by size: 2420 1842 1455 1043 627 397 325 217 BanII G_rGCy'C Cuts at: 1391 2228 2441 3315 4632 5508 5618 5645 1391 Size: 837 213 874 1317 876 110 27 4072 Fragments arranged by size: 4072 1317 876 874 837 213 110 27 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2515 3253 3588 5412 5438 928 Size: 971 616 738 335 1824 26 3816 Fragments arranged by size: 3816 1824 971 738 616 335 26 BbvCI CC'TCA_GC Cuts at: 2029 3352 2029 Size: 1323 7003 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6517 6551 1903 Size: 34 4580 34 3678 Fragments arranged by size: 4580 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6218 7428 6218 Size: 1210 7116 BclI T'GATC_A Cuts at: 1548 4993 5535 1548 Size: 3445 542 4339 Fragments arranged by size: 4339 3445 542 BfrBI ATG'CAT Cuts at: 5009 5009 Size: 8326 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2238 2504 2960 Size: 107 122 71 631 605 566 266 456 Cuts at: 2960 3416 4077 6937 136 Size: 456 661 2860 1525 Fragments arranged by size: 2860 1525 661 631 605 566 456 456 266 122 107 71 BglII A'GATC_T Cuts at: 5215 8321 5215 Size: 3106 5220 BlpI GC'TnA_GC Cuts at: 5743 5743 Size: 8326 Bme1580I G_kGCm'C Cuts at: 1790 2228 2809 2835 2872 4528 6387 7543 1790 Size: 438 581 26 37 1656 1859 1156 2573 Fragments arranged by size: 2573 1859 1656 1156 581 438 37 26 BmgBI CAC'GTC Cuts at: 1318 5323 1318 Size: 4005 4321 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4099 5463 6057 Size: 416 53 83 399 24 2682 1364 594 Cuts at: 6057 7015 442 Size: 958 1753 Fragments arranged by size: 2682 1753 1364 958 594 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2895 1056 Size: 1839 6487 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3505 3640 3652 3505 Size: 135 12 8179 Fragments arranged by size: 8179 135 12 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2368 2435 2817 3360 3509 4750 4917 5259 Size: 1129 67 382 543 149 1241 167 342 Cuts at: 5259 6986 1239 Size: 1727 2579 Fragments arranged by size: 2579 1727 1241 1129 543 382 342 167 149 67 Bpu10I CC'TnA_GC Cuts at: 2029 3352 4550 2029 Size: 1323 1198 5805 Fragments arranged by size: 5805 1323 1198 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4634 4706 5421 6451 7316 7614 4634 Size: 72 715 1030 865 298 5346 Fragments arranged by size: 5346 1030 865 715 298 72 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3457 5289 6989 882 Size: 617 1958 1832 1700 2219 Fragments arranged by size: 2219 1958 1832 1700 617 BsaAI yAC'GTr Cuts at: 493 1204 4061 5623 6117 493 Size: 711 2857 1562 494 2702 Fragments arranged by size: 2857 2702 1562 711 494 BsaBI GATnn'nnATC Cuts at: 8320 8320 Size: 8326 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2241 4128 4575 6133 Size: 53 83 186 936 708 1887 447 1558 Cuts at: 6133 6515 7150 275 Size: 382 635 1451 Fragments arranged by size: 1887 1558 1451 936 708 635 447 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2940 5223 6759 7157 7431 7578 7989 2940 Size: 2283 1536 398 274 147 411 3277 Fragments arranged by size: 3277 2283 1536 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2195 2225 2328 2358 2366 Size: 30 165 30 792 30 103 30 8 Cuts at: 2366 2396 3011 3041 1178 Size: 30 615 30 6463 Fragments arranged by size: 6463 792 615 165 103 30 30 30 30 30 30 8 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2539 3340 3485 3625 3626 3629 5107 1186 Size: 1353 801 145 140 1 3 1478 4405 Fragments arranged by size: 4405 1478 1353 801 145 140 3 1 BseYI C'CCAG_C Cuts at: 2250 2273 2535 2585 2854 3119 3442 3656 3934 Size: 23 262 50 269 265 323 214 278 Cuts at: 3934 3942 4868 5362 5507 7529 8025 2250 Size: 8 926 494 145 2022 496 2551 Fragments arranged by size: 2551 2022 926 496 494 323 278 269 265 262 214 145 50 23 8 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 4932 1163 Size: 573 3196 4557 Fragments arranged by size: 4557 3196 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5776 6537 7565 1540 Size: 275 186 3775 761 1028 2301 Fragments arranged by size: 3775 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2809 2983 3635 3860 5546 6387 6472 7543 2809 Size: 174 652 225 1686 841 85 1071 3592 Fragments arranged by size: 3592 1686 1071 841 652 225 174 85 BsmI GAATG_Cn' Cuts at: 3282 4477 5837 5930 3282 Size: 1195 1360 93 5678 Fragments arranged by size: 5678 1360 1195 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2228 2441 2809 2835 2872 2983 3315 Size: 399 438 213 368 26 37 111 332 Cuts at: 3315 3635 3860 4528 4632 5508 5546 5618 5645 Size: 320 225 668 104 876 38 72 27 Cuts at: 5645 6387 6472 7543 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 876 742 668 438 399 368 332 320 225 213 111 104 85 72 38 37 27 26 BspEI T'CCGG_A Cuts at: 5223 7989 5223 Size: 2766 5560 BspHI T'CATG_A Cuts at: 3721 6215 7945 3721 Size: 2494 1730 4102 Fragments arranged by size: 4102 2494 1730 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2116 4323 4817 5681 844 Size: 1272 2207 494 864 3489 Fragments arranged by size: 3489 2207 1272 864 494 BsrBI CCG'CTC Cuts at: 1394 1823 2382 6213 1394 Size: 429 559 3831 3507 Fragments arranged by size: 3831 3507 559 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2307 6821 6995 65 Size: 1183 1059 4514 174 1396 Fragments arranged by size: 4514 1396 1183 1059 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4038 6970 7157 1700 Size: 110 2228 2932 187 2869 Fragments arranged by size: 2932 2869 2228 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8326 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8326 BssSI C'ACGA_G Cuts at: 6386 7398 6386 Size: 1012 7314 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8326 BstBI TT'CG_AA Cuts at: 2007 7130 2007 Size: 5123 3203 BstEII G'GTnAC_C Cuts at: 1184 3947 1184 Size: 2763 5563 BstXI CCAn_nnnn'nTGG Cuts at: 3442 3484 3442 Size: 42 8284 BstYI r'GATC_y Cuts at: 1972 2634 2760 3007 4320 5215 5656 6023 6408 Size: 662 126 247 1313 895 441 367 385 Cuts at: 6408 6425 7877 7963 7975 8321 1972 Size: 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1313 895 662 441 385 367 346 247 126 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2095 2151 3189 3468 2095 Size: 56 1038 279 6953 Fragments arranged by size: 6953 1038 279 56 BtgI C'CryG_G Cuts at: 513 1065 2022 2298 2517 2529 2541 5080 5191 Size: 552 957 276 219 12 12 2539 111 Cuts at: 5191 513 Size: 3648 Fragments arranged by size: 3648 2539 957 552 276 219 111 12 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7587 Fragments arranged by size: 7587 683 56 BtsI GCAGTG_nn' Cuts at: 2493 4944 5842 6636 6656 2493 Size: 2451 898 794 20 4163 Fragments arranged by size: 4163 2451 898 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8326 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2068 2103 2902 2937 529 Size: 35 1504 35 799 35 5918 Fragments arranged by size: 5918 1504 799 35 35 35 DraI TTT'AAA Cuts at: 5630 5630 Size: 8326 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3257 7333 809 Size: 507 1941 4076 1802 Fragments arranged by size: 4076 1941 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2693 3417 3669 Size: 54 1425 50 275 18 863 724 252 Cuts at: 3669 5773 6662 8291 8 Size: 2104 889 1629 43 Fragments arranged by size: 2104 1629 1425 889 863 724 275 252 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5773 1537 Size: 275 3961 4090 Fragments arranged by size: 4090 3961 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2600 2656 2932 3436 3603 4412 6256 1353 Size: 1247 56 276 504 167 809 1844 3423 Fragments arranged by size: 3423 1844 1247 809 504 276 167 56 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2051 4063 6913 7287 7433 387 Size: 1064 138 462 2012 2850 374 146 1280 Fragments arranged by size: 2850 2012 1280 1064 462 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2368 2435 2583 2759 2817 3360 3509 Size: 393 736 67 148 176 58 543 149 Cuts at: 3509 3567 4550 4750 4917 5259 5323 5424 6389 Size: 58 983 200 167 342 64 101 965 Cuts at: 6389 6986 7773 8041 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 983 965 787 736 597 543 393 342 268 200 176 167 149 148 101 67 64 58 58 EcoNI CCTnn'n_nnAGG Cuts at: 4121 4121 Size: 8326 EcoO109I rG'GnC_Cy Cuts at: 2734 3217 3380 4254 4732 2734 Size: 483 163 874 478 6328 Fragments arranged by size: 6328 874 483 478 163 EcoRI G'AATT_C Cuts at: 5635 6013 5635 Size: 378 7948 EcoRV GAT'ATC Cuts at: 4783 4855 4783 Size: 72 8254 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 5462 5462 Size: 8326 FspI TGC'GCA Cuts at: 4142 6832 4142 Size: 2690 5636 HaeII r_GCGC'y Cuts at: 1678 7473 1678 Size: 5795 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2195 2227 2626 2658 2978 Size: 32 135 32 623 32 399 32 320 Cuts at: 2978 3010 3188 3220 3729 3761 6967 6999 7041 Size: 32 178 32 509 32 3206 32 42 Cuts at: 7041 7073 1373 Size: 32 2626 Fragments arranged by size: 3206 2626 623 509 399 320 178 135 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2821 4744 5670 5916 669 Size: 988 281 883 1923 926 246 3079 Fragments arranged by size: 3079 1923 988 926 883 281 246 HindIII A'AGCT_T Cuts at: 748 5201 748 Size: 4453 3873 HpaI GTT'AAC Cuts at: 5916 5916 Size: 8326 KpnI G_GTAC'C Cuts at: 5651 5651 Size: 8326 MfeI C'AATT_G Cuts at: 5925 5925 Size: 8326 MluI A'CGCG_T Cuts at: 8202 8202 Size: 8326 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3035 3228 3760 7440 7624 1305 Size: 1730 193 532 3680 184 2007 Fragments arranged by size: 3680 2007 1730 532 193 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2695 3419 3671 10 Size: 54 1425 343 863 724 252 4665 Fragments arranged by size: 4665 1425 863 724 343 252 54 MslI CAynn'nnrTG Cuts at: 518 1166 2708 3306 6284 6643 6802 518 Size: 648 1542 598 2978 359 159 2042 Fragments arranged by size: 2978 2042 1542 648 598 359 159 MspA1I CmG'CkG Cuts at: 1606 2582 2858 3442 4299 4875 5742 6419 7567 Size: 976 276 584 857 576 867 677 1148 Cuts at: 7567 7812 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 976 867 857 677 584 576 276 245 NcoI C'CATG_G Cuts at: 513 1065 2022 2517 2529 2541 5191 513 Size: 552 957 495 12 12 2650 3648 Fragments arranged by size: 3648 2650 957 552 495 12 12 NdeI CA'TA_TG Cuts at: 387 387 Size: 8326 NheI G'CTAG_C Cuts at: 1052 2891 1052 Size: 1839 6487 NotI GC'GGCC_GC Cuts at: 5773 5773 Size: 8326 NsiI A_TGCA'T Cuts at: 5011 5011 Size: 8326 NspI r_CATG'y Cuts at: 1132 1229 3305 4943 5684 7229 1132 Size: 97 2076 1638 741 1545 2229 Fragments arranged by size: 2229 2076 1638 1545 741 97 PasI CC'CwG_GG Cuts at: 1348 3822 4728 4910 1348 Size: 2474 906 182 4764 Fragments arranged by size: 4764 2474 906 182 PciI A'CATG_T Cuts at: 7225 7225 Size: 8326 PflMI CCAn_nnn'nTGG Cuts at: 1108 2413 2542 5175 1108 Size: 1305 129 2633 4259 Fragments arranged by size: 4259 2633 1305 129 PfoI T'CCnGG_A Cuts at: 2346 4734 2346 Size: 2388 5938 PmeI GTTT'AAAC Cuts at: 5630 5630 Size: 8326 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2553 2585 3011 3043 6395 6427 7908 7940 2553 Size: 32 426 32 3352 32 1481 32 2939 Fragments arranged by size: 3352 2939 1481 426 32 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3217 3380 4732 3217 Size: 163 1352 6811 Fragments arranged by size: 6811 1352 163 PshAI GACnn'nnGTC Cuts at: 1613 4920 1613 Size: 3307 5019 PsiI TTA'TAA Cuts at: 5896 5896 Size: 8326 PspOMI G'GGCC_C Cuts at: 2224 2224 Size: 8326 PspXI vC'TCGA_Gb Cuts at: 4200 4200 Size: 8326 PstI C_TGCA'G Cuts at: 830 4880 5678 830 Size: 4050 798 3478 Fragments arranged by size: 4050 3478 798 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8326 PvuII CAG'CTG Cuts at: 2858 3442 4875 2858 Size: 584 1433 6309 Fragments arranged by size: 6309 1433 584 SalI G'TCGA_C Cuts at: 1936 5668 1936 Size: 3732 4594 SapI GCTCTTCn'nnn_ Cuts at: 2600 4412 2600 Size: 1812 6514 SbfI CC_TGCA'GG Cuts at: 5678 5678 Size: 8326 ScaI AGT'ACT Cuts at: 6574 8286 6574 Size: 1712 6614 SfcI C'TryA_G Cuts at: 826 1045 2047 2389 3751 4453 4672 4876 5674 Size: 219 1002 342 1362 702 219 204 798 Cuts at: 5674 6809 7490 7681 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1362 1135 1002 798 702 681 342 219 219 204 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8326 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8326 SmaI CCC'GGG Cuts at: 1872 5653 1872 Size: 3781 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4200 4649 4721 5436 6430 7331 Size: 197 926 2257 449 72 715 994 901 Cuts at: 7331 7593 820 Size: 262 1553 Fragments arranged by size: 2257 1553 994 926 901 715 449 262 197 72 SnaBI TAC'GTA Cuts at: 493 5623 6117 493 Size: 5130 494 2702 Fragments arranged by size: 5130 2702 494 SpeI A'CTAG_T Cuts at: 152 3565 152 Size: 3413 4913 SphI G_CATG'C Cuts at: 1132 5684 1132 Size: 4552 3774 SspI AAT'ATT Cuts at: 5 52 6250 5 Size: 47 6198 2081 Fragments arranged by size: 6198 2081 47 StuI AGG'CCT Cuts at: 1517 2232 1517 Size: 715 7611 StyI C'CwwG_G Cuts at: 513 1065 2022 2517 2529 2541 3240 4423 5191 Size: 552 957 495 12 12 699 1183 768 Cuts at: 5191 5765 513 Size: 574 3074 Fragments arranged by size: 3074 1183 957 768 699 574 552 495 12 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2770 3196 5186 6353 6370 6523 6708 Size: 1255 1190 426 1990 1167 17 153 185 Cuts at: 6708 7153 325 Size: 445 1498 Fragments arranged by size: 1990 1498 1255 1190 1167 445 426 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 6572 8284 96 Size: 274 80 33 51 1443 4595 1712 138 Fragments arranged by size: 4595 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2309 2341 2745 2777 2891 2923 8286 8318 2309 Size: 32 404 32 114 32 5363 32 2317 Fragments arranged by size: 5363 2317 404 114 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2209 1316 Size: 893 7433 XbaI T'CTAG_A Cuts at: 3680 5662 3680 Size: 1982 6344 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2515 3664 4688 4774 2515 Size: 1149 1024 86 6067 Fragments arranged by size: 6067 1149 1024 86 XhoI C'TCGA_G Cuts at: 1943 4200 1943 Size: 2257 6069 XmaI C'CCGG_G Cuts at: 1870 5651 1870 Size: 3781 4545 XmnI GAAnn'nnTTC Cuts at: 4088 6455 4088 Size: 2367 5959 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6134 276 Size: 53 83 186 936 4600 2468 Fragments arranged by size: 4600 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BbeI BsiWI BsmBI BstZ17I DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PmlI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI