Restriction map for
pFN21ASDA0173
(Linear) (Six-base) MAPSORT of: KIAA0173.seq Check: 1667 from: 1 to: 3597
KIAA0173.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AccI GT'mk_AC
Cuts at: 0 862 3597
Size: 862 2735
AclI AA'CG_TT
Cuts at: 0 1745 3597
Size: 1745 1852
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 560 736 1544 2527 3300 3401 3597
Size: 560 176 808 983 773 101 196
Fragments arranged by size:
983 808 773 560 196 176 101
AleI CACnn'nnGTG
Cuts at: 0 685 3597
Size: 685 2912
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 703 2939 3597
Size: 703 2236 658
Fragments arranged by size:
2236 703 658
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 988 1020 3597
Size: 988 32 2577
Fragments arranged by size:
2577 988 32
AlwNI CAG_nnn'CTG
Cuts at: 0 85 122 305 390 562 714 1495 2852
Size: 85 37 183 85 172 152 781 1357
Cuts at: 2852 3597
Size: 745
Fragments arranged by size:
1357 781 745 183 172 152 85 85
37
ApaI G_GGCC'C
Cuts at: 0 205 3597
Size: 205 3392
ApaLI G'TGCA_C
Cuts at: 0 782 3597
Size: 782 2815
ApoI r'AATT_y
Cuts at: 0 1673 2963 3074 3597
Size: 1673 1290 111 523
Fragments arranged by size:
1673 1290 523 111
AvaI C'yCGr_G
Cuts at: 0 1772 2177 3597
Size: 1772 405 1420
Fragments arranged by size:
1772 1420 405
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 2233 2266 3597
Size: 2233 33 1331
Fragments arranged by size:
2233 1331 33
BamHI G'GATC_C
Cuts at: 0 984 3597
Size: 984 2613
BanI G'GyrC_C
Cuts at: 0 807 1204 3597
Size: 807 397 2393
Fragments arranged by size:
2393 807 397
BanII G_rGCy'C
Cuts at: 0 205 418 1292 2609 3485 3595 3597
Size: 205 213 874 1317 876 110 2
Fragments arranged by size:
1317 876 874 213 205 110 2
BbsI GAAGACnn'nnnn_
Cuts at: 0 492 1230 1565 3389 3415 3597
Size: 492 738 335 1824 26 182
Fragments arranged by size:
1824 738 492 335 182 26
BbvCI CC'TCA_GC
Cuts at: 0 6 1329 3597
Size: 6 1323 2268
Fragments arranged by size:
2268 1323 6
BclI T'GATC_A
Cuts at: 0 2970 3512 3597
Size: 2970 542 85
Fragments arranged by size:
2970 542 85
BfrBI ATG'CAT
Cuts at: 0 2986 3597
Size: 2986 611
BglI GCCn_nnn'nGGC
Cuts at: 0 215 481 937 1393 2054 3597
Size: 215 266 456 456 661 1543
Fragments arranged by size:
1543 661 456 456 266 215
BglII A'GATC_T
Cuts at: 0 3192 3597
Size: 3192 405
Bme1580I G_kGCm'C
Cuts at: 0 205 786 812 849 2505 3597
Size: 205 581 26 37 1656 1092
Fragments arranged by size:
1656 1092 581 205 37 26
BmgBI CAC'GTC
Cuts at: 0 3300 3597
Size: 3300 297
BmrI ACTGGGnnnn_n'
Cuts at: 0 2076 3440 3597
Size: 2076 1364 157
Fragments arranged by size:
2076 1364 157
BmtI G_CTAG'C
Cuts at: 0 872 3597
Size: 872 2725
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1482 1617 1629 3597
Size: 1482 135 12 1968
Fragments arranged by size:
1968 1482 135 12
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 345 412 794 1337 1486 2727 2894 3236
Size: 345 67 382 543 149 1241 167 342
Cuts at: 3236 3597
Size: 361
Fragments arranged by size:
1241 543 382 361 345 342 167 149
67
Bpu10I CC'TnA_GC
Cuts at: 0 6 1329 2527 3597
Size: 6 1323 1198 1070
Fragments arranged by size:
1323 1198 1070 6
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2611 2683 3398 3597
Size: 2611 72 715 199
Fragments arranged by size:
2611 715 199 72
BsaI GGTCTCn'nnnn_
Cuts at: 0 1434 3266 3597
Size: 1434 1832 331
Fragments arranged by size:
1832 1434 331
BsaAI yAC'GTr
Cuts at: 0 2038 3597
Size: 2038 1559
BsaHI Gr'CG_yC
Cuts at: 0 218 2105 2552 3597
Size: 218 1887 447 1045
Fragments arranged by size:
1887 1045 447 218
BsaWI w'CCGG_w
Cuts at: 0 917 3200 3597
Size: 917 2283 397
Fragments arranged by size:
2283 917 397
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 172 202 305 335 343 373 988 1018
Size: 172 30 103 30 8 30 615 30
Cuts at: 1018 3597
Size: 2579
Fragments arranged by size:
2579 615 172 103 30 30 30 30
8
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 516 1317 1462 1602 1603 1606 3084 3597
Size: 516 801 145 140 1 3 1478 513
Fragments arranged by size:
1478 801 516 513 145 140 3 1
BseYI C'CCAG_C
Cuts at: 0 227 250 512 562 831 1096 1419 1633
Size: 227 23 262 50 269 265 323 214
Cuts at: 1633 1911 1919 2845 3339 3484 3597
Size: 278 8 926 494 145 113
Fragments arranged by size:
926 494 323 278 269 265 262 227
214 145 113 50 23 8
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2909 3597
Size: 2909 688
BsiHKAI G_wGCw'C
Cuts at: 0 786 960 1612 1837 3523 3597
Size: 786 174 652 225 1686 74
Fragments arranged by size:
1686 786 652 225 174 74
BsmI GAATG_Cn'
Cuts at: 0 1259 2454 3597
Size: 1259 1195 1143
Fragments arranged by size:
1259 1195 1143
Bsp1286I G_dGCh'C
Cuts at: 0 205 418 786 812 849 960 1292 1612
Size: 205 213 368 26 37 111 332 320
Cuts at: 1612 1837 2505 2609 3485 3523 3595 3597
Size: 225 668 104 876 38 72 2
Fragments arranged by size:
876 668 368 332 320 225 213 205
111 104 72 38 37 26 2
BspEI T'CCGG_A
Cuts at: 0 3200 3597
Size: 3200 397
BspHI T'CATG_A
Cuts at: 0 1698 3597
Size: 1698 1899
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 93 2300 2794 3597
Size: 93 2207 494 803
Fragments arranged by size:
2207 803 494 93
BsrBI CCG'CTC
Cuts at: 0 359 3597
Size: 359 3238
BsrDI GCAATG_nn'
Cuts at: 0 284 3597
Size: 284 3313
BsrFI r'CCGG_y
Cuts at: 0 2015 3597
Size: 2015 1582
BstEII G'GTnAC_C
Cuts at: 0 1924 3597
Size: 1924 1673
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1419 1461 3597
Size: 1419 42 2136
Fragments arranged by size:
2136 1419 42
BstYI r'GATC_y
Cuts at: 0 611 737 984 2297 3192 3597
Size: 611 126 247 1313 895 405
Fragments arranged by size:
1313 895 611 405 247 126
Bsu36I CC'TnA_GG
Cuts at: 0 72 128 1166 1445 3597
Size: 72 56 1038 279 2152
Fragments arranged by size:
2152 1038 279 72 56
BtgI C'CryG_G
Cuts at: 0 275 494 506 518 3057 3168 3597
Size: 275 219 12 12 2539 111 429
Fragments arranged by size:
2539 429 275 219 111 12 12
BtsI GCAGTG_nn'
Cuts at: 0 470 2921 3597
Size: 470 2451 676
Fragments arranged by size:
2451 676 470
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 45 80 879 914 3597
Size: 45 35 799 35 2683
Fragments arranged by size:
2683 799 45 35 35
DrdI GACnn_nn'nnGTC
Cuts at: 0 1234 3597
Size: 1234 2363
EaeI y'GGCC_r
Cuts at: 0 670 1394 1646 3597
Size: 670 724 252 1951
Fragments arranged by size:
1951 724 670 252
EarI CTCTTCn'nnn_
Cuts at: 0 577 633 909 1413 1580 2389 3597
Size: 577 56 276 504 167 809 1208
Fragments arranged by size:
1208 809 577 504 276 167 56
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 28 2040 3597
Size: 28 2012 1557
Fragments arranged by size:
2012 1557 28
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 345 412 560 736 794 1337 1486 1544
Size: 345 67 148 176 58 543 149 58
Cuts at: 1544 2527 2727 2894 3236 3300 3401 3597
Size: 983 200 167 342 64 101 196
Fragments arranged by size:
983 543 345 342 200 196 176 167
149 148 101 67 64 58 58
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2098 3597
Size: 2098 1499
EcoO109I rG'GnC_Cy
Cuts at: 0 711 1194 1357 2231 2709 3597
Size: 711 483 163 874 478 888
Fragments arranged by size:
888 874 711 483 478 163
EcoRV GAT'ATC
Cuts at: 0 2760 2832 3597
Size: 2760 72 765
Fragments arranged by size:
2760 765 72
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 3439 3597
Size: 3439 158
FspI TGC'GCA
Cuts at: 0 2119 3597
Size: 2119 1478
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 172 204 603 635 955 987 1165 1197
Size: 172 32 399 32 320 32 178 32
Cuts at: 1197 1706 1738 3597
Size: 509 32 1859
Fragments arranged by size:
1859 509 399 320 178 172 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 798 2721 3597
Size: 798 1923 876
Fragments arranged by size:
1923 876 798
HindIII A'AGCT_T
Cuts at: 0 3178 3597
Size: 3178 419
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1012 1205 1737 3597
Size: 1012 193 532 1860
Fragments arranged by size:
1860 1012 532 193
MscI TGG'CCA
Cuts at: 0 672 1396 1648 3597
Size: 672 724 252 1949
Fragments arranged by size:
1949 724 672 252
MslI CAynn'nnrTG
Cuts at: 0 685 1283 3597
Size: 685 598 2314
Fragments arranged by size:
2314 685 598
MspA1I CmG'CkG
Cuts at: 0 559 835 1419 2276 2852 3597
Size: 559 276 584 857 576 745
Fragments arranged by size:
857 745 584 576 559 276
NcoI C'CATG_G
Cuts at: 0 494 506 518 3168 3597
Size: 494 12 12 2650 429
Fragments arranged by size:
2650 494 429 12 12
NheI G'CTAG_C
Cuts at: 0 868 3597
Size: 868 2729
NsiI A_TGCA'T
Cuts at: 0 2988 3597
Size: 2988 609
NspI r_CATG'y
Cuts at: 0 1282 2920 3597
Size: 1282 1638 677
Fragments arranged by size:
1638 1282 677
PasI CC'CwG_GG
Cuts at: 0 1799 2705 2887 3597
Size: 1799 906 182 710
Fragments arranged by size:
1799 906 710 182
PflMI CCAn_nnn'nTGG
Cuts at: 0 390 519 3152 3597
Size: 390 129 2633 445
Fragments arranged by size:
2633 445 390 129
PfoI T'CCnGG_A
Cuts at: 0 323 2711 3597
Size: 323 2388 886
Fragments arranged by size:
2388 886 323
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 530 562 988 1020 3597
Size: 530 32 426 32 2577
Fragments arranged by size:
2577 530 426 32 32
PpuMI rG'GwC_Cy
Cuts at: 0 1194 1357 2709 3597
Size: 1194 163 1352 888
Fragments arranged by size:
1352 1194 888 163
PshAI GACnn'nnGTC
Cuts at: 0 2897 3597
Size: 2897 700
PspOMI G'GGCC_C
Cuts at: 0 201 3597
Size: 201 3396
PspXI vC'TCGA_Gb
Cuts at: 0 2177 3597
Size: 2177 1420
PstI C_TGCA'G
Cuts at: 0 2857 3597
Size: 2857 740
PvuII CAG'CTG
Cuts at: 0 835 1419 2852 3597
Size: 835 584 1433 745
Fragments arranged by size:
1433 835 745 584
SapI GCTCTTCn'nnn_
Cuts at: 0 577 2389 3597
Size: 577 1812 1208
Fragments arranged by size:
1812 1208 577
SfcI C'TryA_G
Cuts at: 0 24 366 1728 2430 2649 2853 3597
Size: 24 342 1362 702 219 204 744
Fragments arranged by size:
1362 744 702 342 219 204 24
SmlI C'TyrA_G
Cuts at: 0 2177 2626 2698 3413 3597
Size: 2177 449 72 715 184
Fragments arranged by size:
2177 715 449 184 72
SpeI A'CTAG_T
Cuts at: 0 1542 3597
Size: 1542 2055
StuI AGG'CCT
Cuts at: 0 209 3597
Size: 209 3388
StyI C'CwwG_G
Cuts at: 0 494 506 518 1217 2400 3168 3597
Size: 494 12 12 699 1183 768 429
Fragments arranged by size:
1183 768 699 494 429 12 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 747 1173 3163 3597
Size: 747 426 1990 434
Fragments arranged by size:
1990 747 434 426
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 286 318 722 754 868 900 3597
Size: 286 32 404 32 114 32 2697
Fragments arranged by size:
2697 404 286 114 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 186 3597
Size: 186 3411
XbaI T'CTAG_A
Cuts at: 0 1657 3597
Size: 1657 1940
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 492 1641 2665 2751 3597
Size: 492 1149 1024 86 846
Fragments arranged by size:
1149 1024 846 492 86
XhoI C'TCGA_G
Cuts at: 0 2177 3597
Size: 2177 1420
XmnI GAAnn'nnTTC
Cuts at: 0 2065 3597
Size: 2065 1532
Enzymes that do cut:
AccI AclI AcuI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AvaI BaeI BamHI BanI BanII BbsI
BbvCI BclI BfrBI BglI BglII Bme1580I BmgBI BmrI
BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaWI BsaXI BseRI BseYI BsgI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BstEII BstXI
BstYI Bsu36I BtgI BtsI CspCI DrdI EaeI EarI
EciI Eco57MI EcoNI EcoO109I EcoRV FalI FspI Hin4I
HincII HindIII MmeI MscI MslI MspA1I NcoI NheI
NsiI NspI PasI PflMI PfoI PpiI PpuMI PshAI
PspOMI PspXI PstI PvuII SapI SfcI SmlI SpeI
StuI StyI TaqII TstI Tth111I XbaI XcmI XhoI
XmnI
Enzymes that do not cut:
AarI AatII Acc65I AfeI AflII AflIII AgeI AhdI
AscI AseI AsiSI AvrII BbeI BcgI BciVI BlpI
BsaBI BsiEI BsiWI BsmBI BsrGI BssHII BssSI BstAPI
BstBI BstZ17I BtgZI ClaI DraI DraIII EagI EcoICRI
EcoRI FseI FspAI HaeII HpaI KasI KpnI MfeI
MluI NaeI NarI NdeI NgoMIV NotI NruI PacI
PciI PmeI PmlI PsiI PsrI PvuI RsrII SacI
SacII SalI SanDI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SphI SrfI SspI SwaI
TatI XmaI ZraI