Restriction map for
pFN21ASDA0172
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# NO CUT ENZYMES ( 86 enzymes) #
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Acc65I AccI AccIII AcsI AgeI
AhdI Aor51HI ApoI AscI Asp700I
Asp718I AspEI AviII BamHI BbeI
BcgI BclI BlpI Bpu1102I BsaBI
BsaWI BseAI BsiEI BsiWI BspDI
BspEI BspLU11I BsrBI BssHII Bst1107I
BstBI BstZ17I CelII ClaI CpoI
DraI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoRI EcoRV FbaI
FseI FspI HaeII KasI KpnI
KspI MamI MfeI MluI MroI
MunI NaeI NarI NdeI NgoMIV
NheI NotI NruI NspV PacI
PaeR7I PinAI PmeI PshAI PvuI
RsrII SacII SalI SbfI ScaI
SfiI SfoI SfuI SgrAI SnaBI
SpeI Sse8387I SwaI XbaI XhoI
XmnI
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# ONE CUT ENZYMES ( 38 enzymes) #
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AatII 4020 GACGTC GACGT^C
AclI 2217 AACGTT AA^CGTT
AcyI 4020 GACGTC G[GA]^CG[CT]C
AflII 2751 CTTAAG C^TTAAG
Alw44I 2038 GTGCAC G^TGCAC
AocI 32 CCTCAGG CC^T[ACGT]AGG
ApaI 1010 GGGCCC GGGCC^C
ApaLI 2038 GTGCAC G^TGCAC
AseI 2995 ATTAAT AT^TAAT
AsnI 2995 ATTAAT AT^TAAT
AvrII 4003 CCTAGG C^CTAGG
BbvCI 2652 GCTGAGG CCTCAGC(-5/-2)
BfrI 2751 CTTAAG C^TTAAG
BlnI 4003 CCTAGG C^CTAGG
BsaHI 4020 GACGTC G[GA]^CG[CT]C
BsmI 3678 GAATGC GAATGC(1/-1)
Bsp1407I 2899 TGTACA T^GTACA
BsrDI 804 CATTGC GCAATG(2/0)
BsrFI 1193 GCCGGT [GA]^CCGG[CT]
BsrGI 2899 TGTACA T^GTACA
BssSI 3358 CACGAG CACGAG(-5/-1)
BstEII 3880 GGTCACC G^GT[ACGT]ACC
BstPI 3880 GGTCACC G^GT[ACGT]ACC
Bsu36I 32 CCTCAGG CC^T[ACGT]AGG
Cfr10I 1193 GCCGGT [GA]^CCGG[CT]
DraIII 524 CACCGGGTG CAC[ACGT][ACGT][ACGT]^GTG
Eco81I 32 CCTCAGG CC^T[ACGT]AGG
EcoO65I 3880 GGTCACC G^GT[ACGT]ACC
Hin1I 4020 GACGTC G[GA]^CG[CT]C
HpaI 19 GTTAAC GTT^AAC
PflMI 2127 CCAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PshBI 2995 ATTAAT AT^TAAT
Psp1406I 2217 AACGTT AA^CGTT
SexAI 1595 ACCTGGT A^CC[AT]GGT
SphI 3274 GCATGC GCATG^C
SspBI 2899 TGTACA T^GTACA
StuI 1160 AGGCCT AGG^CCT
Van91I 2127 CCAGACTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 23 enzymes) #
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AflIII 1604 ACACGT A^C[GA][CT]GT
1606 ACGTGT
AspI 1231 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2196 GACGCGGTC
BbrPI 1605 CACGTG CAC^GTG
3835 CACGTG
BglII 869 AGATCT A^GATCT
1403 AGATCT
BsaAI 1605 CACGTG [CT]AC^GT[GA]
3835 CACGTG
BsgI 2082 CTGCAC GTGCAG(16/14)
1558 GTGCAG
BspHI 2627 TCATGA T^CATGA
2930 TCATGA
BstAPI 1643 GCATCTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
2511 GCAGACACTGC
BstXI 648 CCAAGGTAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3596 CCATCATGTTGG
EcoT22I 1729 ATGCAT ATGCA^T
2894 ATGCAT
HindIII 2549 AAGCTT A^AGCTT
3311 AAGCTT
NcoI 701 CCATGG C^CATGG
3401 CCATGG
NsiI 1729 ATGCAT ATGCA^T
2894 ATGCAT
PflFI 1231 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2196 GACGCGGTC
PmaCI 1605 CACGTG CAC^GTG
3835 CACGTG
PmlI 1605 CACGTG CAC^GTG
3835 CACGTG
RcaI 2627 TCATGA T^CATGA
2930 TCATGA
SapI 2647 GAAGAGC GCTCTTC(1/4)
3047 GCTCTTC
SmaI 1007 CCCGGG CCC^GGG
1940 CCCGGG
SspI 2293 AATATT AAT^ATT
3160 AATATT
Tth111I 1231 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
2196 GACGCGGTC
XmaCI 1007 CCCGGG C^CCGGG
1940 CCCGGG
XmaI 1007 CCCGGG C^CCGGG
1940 CCCGGG
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# 3 OR MORE CUT ENZYMES ( 111 enzymes) #
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AccII AciI AfaI AluI AlwI
AlwNI AspHI AvaI AvaII BalI
BanI BanII BbsI BbvI BciVI
BcnI BfaI BglI BmyI BpmI
BpuAI BsaI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmBI BsmFI
BsoBI Bsp1286I BspMI BsrI BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DrdI DsaI EaeI EarI EcoO109I
EcoRII EcoT14I FauI Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HincII HindII HinfI HpaII HphI
ItaI Ksp632I MaeI MaeII MaeIII
MboI MboII MflI MluNI MnlI
MscI MseI MslI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV NspI PleI PpuMI
PstI PvuII RsaI SacI Sau3AI
Sau96I ScrFI SfaNI SfcI SmlI
StyI TaqI TfiI Tru9I TseI
Tsp45I Tsp509I TspRI TthHB8I XcmI
XhoII