(Circular) (Six-base) MAPSORT of: pFN21ASDA0172.seq Check: 6683 from: 1 to: 8785 pFN21ASDA0172 8785 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2057 2808 2851 2057 Size: 751 43 7991 Fragments arranged by size: 7991 751 43 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6047 6595 278 Size: 53 83 186 936 4511 548 2468 Fragments arranged by size: 4511 2468 936 548 186 83 53 AccI GT'mk_AC Cuts at: 1937 6128 1937 Size: 4191 4594 Acc65I G'GTAC_C Cuts at: 6106 6106 Size: 8785 AclI AA'CG_TT Cuts at: 1563 4241 6912 7285 1563 Size: 2678 2671 373 3063 Fragments arranged by size: 3063 2678 2671 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2642 2864 3390 4689 4742 4964 4988 6848 Size: 1010 222 526 1299 53 222 24 1860 Cuts at: 6848 8232 8500 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1860 1384 1299 1010 526 268 222 222 53 24 AflII C'TTAA_G Cuts at: 820 1017 4774 820 Size: 197 3757 4831 Fragments arranged by size: 4831 3757 197 AflIII A'CryG_T Cuts at: 1203 3627 3629 7684 8661 1203 Size: 2424 2 4055 977 1327 Fragments arranged by size: 4055 2424 1327 977 2 AgeI A'CCGG_T Cuts at: 7616 7616 Size: 8785 AhdI GACnn_n'nnGTC Cuts at: 7514 7514 Size: 8785 AleI CACnn'nnGTG Cuts at: 1166 2774 1166 Size: 1608 7177 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 8785 AlwNI CAG_nnn'CTG Cuts at: 3177 3285 3819 4175 4519 4540 6528 8100 3177 Size: 108 534 356 344 21 1988 1572 3862 Fragments arranged by size: 3862 1988 1572 534 356 344 108 21 ApaI G_GGCC'C Cuts at: 3037 3037 Size: 8785 ApaLI G'TGCA_C Cuts at: 4061 6842 7998 4061 Size: 2781 1156 4848 Fragments arranged by size: 4848 2781 1156 ApoI r'AATT_y Cuts at: 1493 6094 6320 6472 8581 1493 Size: 4601 226 152 2109 1697 Fragments arranged by size: 4601 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5019 7339 160 Size: 872 3987 2320 1606 Fragments arranged by size: 3987 2320 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8785 AvaI C'yCGr_G Cuts at: 1870 1943 3030 3963 5151 6110 1870 Size: 73 1087 933 1188 959 4545 Fragments arranged by size: 4545 1188 1087 959 933 73 AvrII C'CTAG_G Cuts at: 6026 6026 Size: 8785 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2659 2692 2659 Size: 33 8752 BamHI G'GATC_C Cuts at: 6115 6482 6115 Size: 367 8418 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2594 3233 5169 6020 6106 Size: 325 217 627 807 639 1936 851 86 Cuts at: 6106 7561 618 Size: 1455 1842 Fragments arranged by size: 1936 1842 1455 851 807 639 627 325 217 86 BanII G_rGCy'C Cuts at: 1391 2735 2759 2792 3037 3198 3393 4604 5825 Size: 1344 24 33 245 161 195 1211 1221 Cuts at: 5825 6104 1391 Size: 279 4072 Fragments arranged by size: 4072 1344 1221 1211 279 245 195 161 33 24 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2518 4292 5151 6047 928 Size: 971 619 1774 859 896 3666 Fragments arranged by size: 3666 1774 971 896 859 619 BbvCI CC'TCA_GC Cuts at: 4676 4676 Size: 8785 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 6976 7010 1903 Size: 34 5039 34 3678 Fragments arranged by size: 5039 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2660 4520 5121 6677 7887 2660 Size: 1860 601 1556 1210 3558 Fragments arranged by size: 3558 1860 1556 1210 601 BclI T'GATC_A Cuts at: 1548 1548 Size: 8785 BfrBI ATG'CAT Cuts at: 3754 4919 3754 Size: 1165 7620 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3539 4010 5792 Size: 107 122 71 631 605 1867 471 1782 Cuts at: 5792 7396 136 Size: 1604 1525 Fragments arranged by size: 1867 1782 1604 1525 631 605 471 122 107 71 BglII A'GATC_T Cuts at: 2892 3426 8780 2892 Size: 534 5354 2897 Fragments arranged by size: 5354 2897 534 BlpI GC'TnA_GC Cuts at: 6202 6202 Size: 8785 Bme1580I G_kGCm'C Cuts at: 1790 2333 2597 3037 4065 5172 5859 6023 6846 Size: 543 264 440 1028 1107 687 164 823 Cuts at: 6846 8002 1790 Size: 1156 2573 Fragments arranged by size: 2573 1156 1107 1028 823 687 543 440 264 164 BmgBI CAC'GTC Cuts at: 1318 3931 1318 Size: 2613 6172 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2768 6004 6516 Size: 416 53 83 399 24 1351 3236 512 Cuts at: 6516 7474 442 Size: 958 1753 Fragments arranged by size: 3236 1753 1351 958 512 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8785 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3205 3976 3205 Size: 771 8014 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2417 2867 2873 3173 3262 4466 4860 5360 Size: 1178 450 6 300 89 1204 394 500 Cuts at: 5360 7445 1239 Size: 2085 2579 Fragments arranged by size: 2579 2085 1204 1178 500 450 394 300 89 6 Bpu10I CC'TnA_GC Cuts at: 2753 2758 3197 3708 4676 4797 2753 Size: 5 439 511 968 121 6741 Fragments arranged by size: 6741 968 511 439 121 5 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3854 4446 6910 7775 8073 3854 Size: 592 2464 865 298 4566 Fragments arranged by size: 4566 2464 865 592 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2079 2117 2393 2495 2932 3080 3585 Size: 617 580 38 276 102 437 148 505 Cuts at: 3585 4346 7448 882 Size: 761 3102 2219 Fragments arranged by size: 3102 2219 761 617 580 505 437 276 148 102 38 BsaAI yAC'GTr Cuts at: 493 1204 3630 5860 6082 6576 493 Size: 711 2426 2230 222 494 2702 Fragments arranged by size: 2702 2426 2230 711 494 222 BsaBI GATnn'nnATC Cuts at: 8779 8779 Size: 8785 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6044 6592 6974 7609 Size: 53 83 186 936 4511 548 382 635 Cuts at: 7609 275 Size: 1451 Fragments arranged by size: 4511 1451 936 635 548 382 186 83 53 BsaWI w'CCGG_w Cuts at: 7218 7616 7890 8037 8448 7218 Size: 398 274 147 411 7555 Fragments arranged by size: 7555 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2353 2383 2445 2475 3260 Size: 30 165 30 950 30 62 30 785 Cuts at: 3260 3290 4190 4220 4858 4888 5807 5837 1178 Size: 30 900 30 638 30 919 30 4126 Fragments arranged by size: 4126 950 919 900 785 638 165 62 30 30 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2870 3086 3178 3254 3857 4198 5141 5144 Size: 1684 216 92 76 603 341 943 3 Cuts at: 5144 5147 5150 1186 Size: 3 3 4821 Fragments arranged by size: 4821 1684 943 603 341 216 92 76 3 3 3 BseYI C'CCAG_C Cuts at: 2701 3012 3543 3767 4455 4791 5886 7988 8484 Size: 311 531 224 688 336 1095 2102 496 Cuts at: 8484 2701 Size: 3002 Fragments arranged by size: 3002 2102 1095 688 531 496 336 311 224 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3602 4090 1163 Size: 573 1866 488 5858 Fragments arranged by size: 5858 1866 573 488 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6235 6996 8024 1540 Size: 275 186 4234 761 1028 2301 Fragments arranged by size: 4234 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2735 2759 2883 3104 3198 3393 4065 5853 6846 Size: 24 124 221 94 195 672 1788 993 Cuts at: 6846 6931 8002 2735 Size: 85 1071 3518 Fragments arranged by size: 3518 1788 1071 993 672 221 195 124 94 85 24 BsmI GAATG_Cn' Cuts at: 5707 6296 6389 5707 Size: 589 93 8103 Fragments arranged by size: 8103 589 93 BsmBI CGTCTCn'nnnn_ Cuts at: 4076 4154 6051 4076 Size: 78 1897 6810 Fragments arranged by size: 6810 1897 78 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2333 2597 2735 2759 2792 2883 3037 Size: 399 543 264 138 24 33 91 154 Cuts at: 3037 3104 3198 3393 4065 4604 5172 5825 5853 Size: 67 94 195 672 539 568 653 28 Cuts at: 5853 5859 6023 6104 6846 6931 8002 1391 Size: 6 164 81 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 672 653 568 543 539 399 264 195 164 154 138 94 91 85 81 67 33 28 24 6 BspEI T'CCGG_A Cuts at: 8448 8448 Size: 8785 BspHI T'CATG_A Cuts at: 4650 4953 6674 8404 4650 Size: 303 1721 1730 5031 Fragments arranged by size: 5031 1730 1721 303 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2057 2808 2851 5215 6140 844 Size: 1213 751 43 2364 925 3489 Fragments arranged by size: 3489 2364 1213 925 751 43 BsrBI CCG'CTC Cuts at: 1394 1823 6672 1394 Size: 429 4849 3507 Fragments arranged by size: 4849 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2826 7280 7454 65 Size: 1183 1578 4454 174 1396 Fragments arranged by size: 4454 1578 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3216 7429 7616 1700 Size: 110 1406 4213 187 2869 Fragments arranged by size: 4213 2869 1406 187 110 BsrGI T'GTAC_A Cuts at: 96 4922 96 Size: 4826 3959 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8785 BssSI C'ACGA_G Cuts at: 5381 6845 7857 5381 Size: 1464 1012 6309 Fragments arranged by size: 6309 1464 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3672 4540 794 Size: 2878 868 5039 Fragments arranged by size: 5039 2878 868 BstBI TT'CG_AA Cuts at: 2007 7589 2007 Size: 5582 3203 BstEII G'GTnAC_C Cuts at: 1184 5903 1184 Size: 4719 4066 BstXI CCAn_nnnn'nTGG Cuts at: 2678 5626 2678 Size: 2948 5837 BstYI r'GATC_y Cuts at: 1972 2763 2892 3162 3426 6115 6482 6867 6884 Size: 791 129 270 264 2689 367 385 17 Cuts at: 6884 8336 8422 8434 8780 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 2689 1977 1452 791 385 367 346 270 264 129 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2056 2056 Size: 8785 BtgI C'CryG_G Cuts at: 513 1065 2022 2724 3645 5424 5826 513 Size: 552 957 702 921 1779 402 3472 Fragments arranged by size: 3472 1779 957 921 702 552 402 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 5953 487 Size: 683 56 4727 3319 Fragments arranged by size: 4727 3319 683 56 BtsI GCAGTG_nn' Cuts at: 2056 2333 3582 4020 4538 5928 6301 7095 7115 Size: 277 1249 438 518 1390 373 794 20 Cuts at: 7115 2056 Size: 3726 Fragments arranged by size: 3726 1390 1249 794 518 438 373 277 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8785 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2953 2988 4359 4394 529 Size: 35 2389 35 1371 35 4920 Fragments arranged by size: 4920 2389 1371 35 35 35 DraI TTT'AAA Cuts at: 6089 6089 Size: 8785 DraIII CAC_nnn'GTG Cuts at: 2552 2552 Size: 8785 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3599 3935 4294 7792 809 Size: 507 2283 336 359 3498 1802 Fragments arranged by size: 3498 2283 1802 507 359 336 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2574 2823 4086 Size: 54 1425 50 275 18 744 249 1263 Cuts at: 4086 4252 6232 7121 8750 8 Size: 166 1980 889 1629 43 Fragments arranged by size: 1980 1629 1425 1263 889 744 275 249 166 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6232 1537 Size: 275 4420 4090 Fragments arranged by size: 4420 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2210 2904 4665 5077 5118 6715 1353 Size: 857 694 1761 412 41 1597 3423 Fragments arranged by size: 3423 1761 1597 857 694 412 41 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2236 2729 7372 7746 7892 387 Size: 1064 138 647 493 4643 374 146 1280 Fragments arranged by size: 4643 1280 1064 647 493 374 146 138 EcoICRI GAG'CTC Cuts at: 2733 2757 3196 3391 2733 Size: 24 439 195 8127 Fragments arranged by size: 8127 439 195 24 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2417 2642 2864 2867 2873 3173 3262 Size: 393 785 225 222 3 6 300 89 Cuts at: 3262 3390 4466 4689 4742 4860 4964 4988 5360 Size: 128 1076 223 53 118 104 24 372 Cuts at: 5360 6848 7445 8232 8500 1239 Size: 1488 597 787 268 1524 Fragments arranged by size: 1524 1488 1076 787 785 597 393 372 300 268 225 223 222 128 118 104 89 53 24 6 3 EcoO109I rG'GnC_Cy Cuts at: 3282 3639 3704 4753 4812 5776 3282 Size: 357 65 1049 59 964 6291 Fragments arranged by size: 6291 1049 964 357 65 59 EcoRI G'AATT_C Cuts at: 6094 6472 6094 Size: 378 8407 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3464 3895 4487 5091 3464 Size: 431 592 604 7158 Fragments arranged by size: 7158 604 592 431 FspI TGC'GCA Cuts at: 7291 7291 Size: 8785 HaeII r_GCGC'y Cuts at: 1678 7932 1678 Size: 6254 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2193 2225 3203 3235 4190 Size: 32 135 32 621 32 978 32 955 Cuts at: 4190 4222 4259 4291 5042 5054 5074 5086 5347 Size: 32 37 32 751 12 20 12 261 Cuts at: 5347 5379 5807 5839 7426 7458 7500 7532 1373 Size: 32 428 32 1587 32 42 32 2626 Fragments arranged by size: 2626 1587 978 955 751 621 428 261 135 42 37 32 32 32 32 32 32 32 32 32 32 20 12 12 HincII GTy'rAC Cuts at: 669 1657 1938 2044 3526 3928 4380 5803 5995 Size: 988 281 106 1482 402 452 1423 192 Cuts at: 5995 6129 6375 669 Size: 134 246 3079 Fragments arranged by size: 3079 1482 1423 988 452 402 281 246 192 134 106 HindIII A'AGCT_T Cuts at: 748 4572 5334 748 Size: 3824 762 4199 Fragments arranged by size: 4199 3824 762 HpaI GTT'AAC Cuts at: 2044 6375 2044 Size: 4331 4454 KpnI G_GTAC'C Cuts at: 6110 6110 Size: 8785 MfeI C'AATT_G Cuts at: 6384 6384 Size: 8785 MluI A'CGCG_T Cuts at: 8661 8661 Size: 8785 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2331 3418 4216 5565 7899 8083 1305 Size: 1026 1087 798 1349 2334 184 2007 Fragments arranged by size: 2334 2007 1349 1087 1026 798 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2576 2825 4254 10 Size: 54 1425 343 744 249 1429 4541 Fragments arranged by size: 4541 1429 1425 744 343 249 54 MslI CAynn'nnrTG Cuts at: 518 1166 2540 2774 2876 3260 4013 6743 7102 Size: 648 1374 234 102 384 753 2730 359 Cuts at: 7102 7261 518 Size: 159 2042 Fragments arranged by size: 2730 2042 1374 753 648 384 359 234 159 102 MspA1I CmG'CkG Cuts at: 1606 2440 2705 2935 3016 3177 3299 4280 4907 Size: 834 265 230 81 161 122 981 627 Cuts at: 4907 5642 6201 6878 8026 8271 1606 Size: 735 559 677 1148 245 2120 Fragments arranged by size: 2120 1148 981 834 735 677 627 559 265 245 230 161 122 81 NcoI C'CATG_G Cuts at: 513 1065 2022 2724 5424 513 Size: 552 957 702 2700 3874 Fragments arranged by size: 3874 2700 957 702 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8785 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8785 NotI GC'GGCC_GC Cuts at: 6232 6232 Size: 8785 NsiI A_TGCA'T Cuts at: 3756 4921 3756 Size: 1165 7620 NspI r_CATG'y Cuts at: 1132 1229 3145 4923 5301 5665 6143 7688 1132 Size: 97 1916 1778 378 364 478 1545 2229 Fragments arranged by size: 2229 1916 1778 1545 478 378 364 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8785 PciI A'CATG_T Cuts at: 7684 7684 Size: 8785 PflMI CCAn_nnn'nTGG Cuts at: 1108 4156 1108 Size: 3048 5737 PfoI T'CCnGG_A Cuts at: 3165 5808 3165 Size: 2643 6142 PmeI GTTT'AAAC Cuts at: 6089 6089 Size: 8785 PmlI CAC'GTG Cuts at: 3630 5860 3630 Size: 2230 6555 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2011 2043 6854 6886 8367 8399 2011 Size: 32 4811 32 1481 32 2397 Fragments arranged by size: 4811 2397 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3282 3639 4812 3282 Size: 357 1173 7255 Fragments arranged by size: 7255 1173 357 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 8785 PsiI TTA'TAA Cuts at: 6355 6355 Size: 8785 PspOMI G'GGCC_C Cuts at: 3033 3033 Size: 8785 PstI C_TGCA'G Cuts at: 830 2805 3501 4030 6137 830 Size: 1975 696 529 2107 3478 Fragments arranged by size: 3478 2107 1975 696 529 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8785 PvuII CAG'CTG Cuts at: 2440 2705 2935 3016 3177 3299 4280 2440 Size: 265 230 81 161 122 981 6945 Fragments arranged by size: 6945 981 265 230 161 122 81 SacI G_AGCT'C Cuts at: 2735 2759 3198 3393 2735 Size: 24 439 195 8127 Fragments arranged by size: 8127 439 195 24 SalI G'TCGA_C Cuts at: 1936 6127 1936 Size: 4191 4594 SapI GCTCTTCn'nnn_ Cuts at: 4665 5077 4665 Size: 412 8373 SbfI CC_TGCA'GG Cuts at: 6137 6137 Size: 8785 ScaI AGT'ACT Cuts at: 7033 8745 7033 Size: 1712 7073 SexAI A'CCwGG_T Cuts at: 3618 3618 Size: 8785 SfcI C'TryA_G Cuts at: 826 1045 2801 2992 3300 3497 4026 4176 4356 Size: 219 1756 191 308 197 529 150 180 Cuts at: 4356 4431 4555 6133 7268 7949 8140 826 Size: 75 124 1578 1135 681 191 1471 Fragments arranged by size: 1756 1578 1471 1135 681 529 308 219 197 191 191 180 150 124 75 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8785 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8785 SmaI CCC'GGG Cuts at: 1872 3032 3965 6112 1872 Size: 1160 933 2147 4545 Fragments arranged by size: 4545 2147 1160 933 SmlI C'TyrA_G Cuts at: 820 1017 1943 3869 4461 4774 6889 7790 8052 Size: 197 926 1926 592 313 2115 901 262 Cuts at: 8052 820 Size: 1553 Fragments arranged by size: 2115 1926 1553 926 901 592 313 262 197 SnaBI TAC'GTA Cuts at: 493 6082 6576 493 Size: 5589 494 2702 Fragments arranged by size: 5589 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8785 SphI G_CATG'C Cuts at: 1132 5301 6143 1132 Size: 4169 842 3774 Fragments arranged by size: 4169 3774 842 SspI AAT'ATT Cuts at: 5 52 4318 5185 6709 5 Size: 47 4266 867 1524 2081 Fragments arranged by size: 4266 2081 1524 867 47 StuI AGG'CCT Cuts at: 1517 3185 1517 Size: 1668 7117 StyI C'CwwG_G Cuts at: 513 1065 2022 2671 2724 5424 6026 6224 513 Size: 552 957 649 53 2700 602 198 3074 Fragments arranged by size: 3074 2700 957 649 602 552 198 53 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3221 3476 3774 4163 6074 6812 6829 Size: 1255 1641 255 298 389 1911 738 17 Cuts at: 6829 6982 7167 7612 325 Size: 153 185 445 1498 Fragments arranged by size: 1911 1641 1498 1255 738 445 389 298 255 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3442 4922 4937 Size: 274 80 33 51 1443 1465 1480 15 Cuts at: 4937 7031 8743 96 Size: 2094 1712 138 Fragments arranged by size: 2094 1712 1480 1465 1443 274 138 80 51 33 15 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2445 2477 3260 3292 8745 8777 2445 Size: 32 783 32 5453 32 2453 Fragments arranged by size: 5453 2453 783 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3257 4222 1316 Size: 1941 965 5879 Fragments arranged by size: 5879 1941 965 XbaI T'CTAG_A Cuts at: 6121 6121 Size: 8785 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2494 2660 2722 3595 4904 5422 2494 Size: 166 62 873 1309 518 5857 Fragments arranged by size: 5857 1309 873 518 166 62 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8785 XmaI C'CCGG_G Cuts at: 1870 3030 3963 6110 1870 Size: 1160 933 2147 4545 Fragments arranged by size: 4545 2147 1160 933 XmnI GAAnn'nnTTC Cuts at: 6914 6914 Size: 8785 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6045 6593 276 Size: 53 83 186 936 4511 548 2468 Fragments arranged by size: 4511 2468 936 548 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI BbeI BsiWI BstZ17I EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI