Restriction map for
pFN21ASDA0172
(Linear) (Six-base) MAPSORT of: KIAA0172.seq Check: 7044 from: 1 to: 4056
KIAA0172.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 34 785 828 4056
Size: 34 751 43 3228
Fragments arranged by size:
3228 751 43 34
AatII G_ACGT'C
Cuts at: 0 4024 4056
Size: 4024 32
AclI AA'CG_TT
Cuts at: 0 2218 4056
Size: 2218 1838
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 619 841 1367 2666 2719 2941 2965 4056
Size: 619 222 526 1299 53 222 24 1091
Fragments arranged by size:
1299 1091 619 526 222 222 53 24
AflII C'TTAA_G
Cuts at: 0 2751 4056
Size: 2751 1305
AflIII A'CryG_T
Cuts at: 0 1604 1606 4056
Size: 1604 2 2450
Fragments arranged by size:
2450 1604 2
AleI CACnn'nnGTG
Cuts at: 0 751 4056
Size: 751 3305
AlwNI CAG_nnn'CTG
Cuts at: 0 1154 1262 1796 2152 2496 2517 4056
Size: 1154 108 534 356 344 21 1539
Fragments arranged by size:
1539 1154 534 356 344 108 21
ApaI G_GGCC'C
Cuts at: 0 1014 4056
Size: 1014 3042
ApaLI G'TGCA_C
Cuts at: 0 2038 4056
Size: 2038 2018
AseI AT'TA_AT
Cuts at: 0 2996 4056
Size: 2996 1060
AvaI C'yCGr_G
Cuts at: 0 1007 1940 3128 4056
Size: 1007 933 1188 928
Fragments arranged by size:
1188 1007 933 928
AvrII C'CTAG_G
Cuts at: 0 4003 4056
Size: 4003 53
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 636 669 4056
Size: 636 33 3387
Fragments arranged by size:
3387 636 33
BanI G'GyrC_C
Cuts at: 0 571 1210 3146 3997 4056
Size: 571 639 1936 851 59
Fragments arranged by size:
1936 851 639 571 59
BanII G_rGCy'C
Cuts at: 0 712 736 769 1014 1175 1370 2581 3802
Size: 712 24 33 245 161 195 1211 1221
Cuts at: 3802 4056
Size: 254
Fragments arranged by size:
1221 1211 712 254 245 195 161 33
24
BbsI GAAGACnn'nnnn_
Cuts at: 0 495 2269 3128 4024 4056
Size: 495 1774 859 896 32
Fragments arranged by size:
1774 896 859 495 32
BbvCI CC'TCA_GC
Cuts at: 0 2653 4056
Size: 2653 1403
BciVI GTATCCnnnnn_n'
Cuts at: 0 637 2497 3098 4056
Size: 637 1860 601 958
Fragments arranged by size:
1860 958 637 601
BfrBI ATG'CAT
Cuts at: 0 1731 2896 4056
Size: 1731 1165 1160
Fragments arranged by size:
1731 1165 1160
BglI GCCn_nnn'nGGC
Cuts at: 0 1516 1987 3769 4056
Size: 1516 471 1782 287
Fragments arranged by size:
1782 1516 471 287
BglII A'GATC_T
Cuts at: 0 869 1403 4056
Size: 869 534 2653
Fragments arranged by size:
2653 869 534
Bme1580I G_kGCm'C
Cuts at: 0 310 574 1014 2042 3149 3836 4000 4056
Size: 310 264 440 1028 1107 687 164 56
Fragments arranged by size:
1107 1028 687 440 310 264 164 56
BmgBI CAC'GTC
Cuts at: 0 1908 4056
Size: 1908 2148
BmrI ACTGGGnnnn_n'
Cuts at: 0 745 3981 4056
Size: 745 3236 75
Fragments arranged by size:
3236 745 75
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 1182 1953 4056
Size: 1182 771 2103
Fragments arranged by size:
2103 1182 771
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 844 850 1150 1239 2443 2837 3337
Size: 394 450 6 300 89 1204 394 500
Cuts at: 3337 4056
Size: 719
Fragments arranged by size:
1204 719 500 450 394 394 300 89
6
Bpu10I CC'TnA_GC
Cuts at: 0 730 735 1174 1685 2653 2774 4056
Size: 730 5 439 511 968 121 1282
Fragments arranged by size:
1282 968 730 511 439 121 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1831 2423 4056
Size: 1831 592 1633
Fragments arranged by size:
1831 1633 592
BsaI GGTCTCn'nnnn_
Cuts at: 0 56 94 370 472 909 1057 1562 2323
Size: 56 38 276 102 437 148 505 761
Cuts at: 2323 4056
Size: 1733
Fragments arranged by size:
1733 761 505 437 276 148 102 56
38
BsaAI yAC'GTr
Cuts at: 0 1607 3837 4056
Size: 1607 2230 219
Fragments arranged by size:
2230 1607 219
BsaHI Gr'CG_yC
Cuts at: 0 4021 4056
Size: 4021 35
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 330 360 422 452 1237 1267 2167 2197
Size: 330 30 62 30 785 30 900 30
Cuts at: 2197 2835 2865 3784 3814 4056
Size: 638 30 919 30 242
Fragments arranged by size:
919 900 785 638 330 242 62 30
30 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 847 1063 1155 1231 1834 2175 3118 3121
Size: 847 216 92 76 603 341 943 3
Cuts at: 3121 3124 3127 4056
Size: 3 3 929
Fragments arranged by size:
943 929 847 603 341 216 92 76
3 3 3
BseYI C'CCAG_C
Cuts at: 0 678 989 1520 1744 2432 2768 3863 4056
Size: 678 311 531 224 688 336 1095 193
Fragments arranged by size:
1095 688 678 531 336 311 224 193
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1579 2067 4056
Size: 1579 488 1989
Fragments arranged by size:
1989 1579 488
BsiHKAI G_wGCw'C
Cuts at: 0 712 736 860 1081 1175 1370 2042 3830
Size: 712 24 124 221 94 195 672 1788
Cuts at: 3830 4056
Size: 226
Fragments arranged by size:
1788 712 672 226 221 195 124 94
24
BsmI GAATG_Cn'
Cuts at: 0 3684 4056
Size: 3684 372
BsmBI CGTCTCn'nnnn_
Cuts at: 0 2053 2131 4028 4056
Size: 2053 78 1897 28
Fragments arranged by size:
2053 1897 78 28
Bsp1286I G_dGCh'C
Cuts at: 0 310 574 712 736 769 860 1014 1081
Size: 310 264 138 24 33 91 154 67
Cuts at: 1081 1175 1370 2042 2581 3149 3802 3830 3836
Size: 94 195 672 539 568 653 28 6
Cuts at: 3836 4000 4056
Size: 164 56
Fragments arranged by size:
672 653 568 539 310 264 195 164
154 138 94 91 67 56 33 28
24 6
BspHI T'CATG_A
Cuts at: 0 2627 2930 4056
Size: 2627 303 1126
Fragments arranged by size:
2627 1126 303
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 34 785 828 3192 4056
Size: 34 751 43 2364 864
Fragments arranged by size:
2364 864 751 43 34
BsrDI GCAATG_nn'
Cuts at: 0 803 4056
Size: 803 3253
BsrFI r'CCGG_y
Cuts at: 0 1193 4056
Size: 1193 2863
BsrGI T'GTAC_A
Cuts at: 0 2899 4056
Size: 2899 1157
BssSI C'ACGA_G
Cuts at: 0 3358 4056
Size: 3358 698
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1649 2517 4056
Size: 1649 868 1539
Fragments arranged by size:
1649 1539 868
BstEII G'GTnAC_C
Cuts at: 0 3880 4056
Size: 3880 176
BstXI CCAn_nnnn'nTGG
Cuts at: 0 655 3603 4056
Size: 655 2948 453
Fragments arranged by size:
2948 655 453
BstYI r'GATC_y
Cuts at: 0 740 869 1139 1403 4056
Size: 740 129 270 264 2653
Fragments arranged by size:
2653 740 270 264 129
Bsu36I CC'TnA_GG
Cuts at: 0 33 4056
Size: 33 4023
BtgI C'CryG_G
Cuts at: 0 701 1622 3401 3803 4056
Size: 701 921 1779 402 253
Fragments arranged by size:
1779 921 701 402 253
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 3930 4056
Size: 3930 126
BtsI GCAGTG_nn'
Cuts at: 0 33 310 1559 1997 2515 3905 4056
Size: 33 277 1249 438 518 1390 151
Fragments arranged by size:
1390 1249 518 438 277 151 33
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 930 965 2336 2371 4056
Size: 930 35 1371 35 1685
Fragments arranged by size:
1685 1371 930 35 35
DraIII CAC_nnn'GTG
Cuts at: 0 529 4056
Size: 529 3527
DrdI GACnn_nn'nnGTC
Cuts at: 0 1576 1912 2271 4056
Size: 1576 336 359 1785
Fragments arranged by size:
1785 1576 359 336
EaeI y'GGCC_r
Cuts at: 0 551 800 2063 2229 4056
Size: 551 249 1263 166 1827
Fragments arranged by size:
1827 1263 551 249 166
EarI CTCTTCn'nnn_
Cuts at: 0 187 881 2642 3054 3095 4056
Size: 187 694 1761 412 41 961
Fragments arranged by size:
1761 961 694 412 187 41
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 213 706 4056
Size: 213 493 3350
Fragments arranged by size:
3350 493 213
EcoICRI GAG'CTC
Cuts at: 0 710 734 1173 1368 4056
Size: 710 24 439 195 2688
Fragments arranged by size:
2688 710 439 195 24
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 394 619 841 844 850 1150 1239 1367
Size: 394 225 222 3 6 300 89 128
Cuts at: 1367 2443 2666 2719 2837 2941 2965 3337 4056
Size: 1076 223 53 118 104 24 372 719
Fragments arranged by size:
1076 719 394 372 300 225 223 222
128 118 104 89 53 24 6 3
EcoO109I rG'GnC_Cy
Cuts at: 0 1259 1616 1681 2730 2789 3753 4056
Size: 1259 357 65 1049 59 964 303
Fragments arranged by size:
1259 1049 964 357 303 65 59
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1441 1872 2464 3068 4056
Size: 1441 431 592 604 988
Fragments arranged by size:
1441 988 604 592 431
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 170 202 1180 1212 2167 2199 2236 2268
Size: 170 32 978 32 955 32 37 32
Cuts at: 2268 3019 3031 3051 3063 3324 3356 3784 3816
Size: 751 12 20 12 261 32 428 32
Cuts at: 3816 4056
Size: 240
Fragments arranged by size:
978 955 751 428 261 240 170 37
32 32 32 32 32 32 20 12
12
HincII GTy'rAC
Cuts at: 0 21 1503 1905 2357 3780 3972 4056
Size: 21 1482 402 452 1423 192 84
Fragments arranged by size:
1482 1423 452 402 192 84 21
HindIII A'AGCT_T
Cuts at: 0 2549 3311 4056
Size: 2549 762 745
Fragments arranged by size:
2549 762 745
HpaI GTT'AAC
Cuts at: 0 21 4056
Size: 21 4035
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 308 1395 2193 3542 4056
Size: 308 1087 798 1349 514
Fragments arranged by size:
1349 1087 798 514 308
MscI TGG'CCA
Cuts at: 0 553 802 2231 4056
Size: 553 249 1429 1825
Fragments arranged by size:
1825 1429 553 249
MslI CAynn'nnrTG
Cuts at: 0 517 751 853 1237 1990 4056
Size: 517 234 102 384 753 2066
Fragments arranged by size:
2066 753 517 384 234 102
MspA1I CmG'CkG
Cuts at: 0 417 682 912 993 1154 1276 2257 2884
Size: 417 265 230 81 161 122 981 627
Cuts at: 2884 3619 4056
Size: 735 437
Fragments arranged by size:
981 735 627 437 417 265 230 161
122 81
NcoI C'CATG_G
Cuts at: 0 701 3401 4056
Size: 701 2700 655
Fragments arranged by size:
2700 701 655
NsiI A_TGCA'T
Cuts at: 0 1733 2898 4056
Size: 1733 1165 1158
Fragments arranged by size:
1733 1165 1158
NspI r_CATG'y
Cuts at: 0 1122 2900 3278 3642 4056
Size: 1122 1778 378 364 414
Fragments arranged by size:
1778 1122 414 378 364
PflMI CCAn_nnn'nTGG
Cuts at: 0 2133 4056
Size: 2133 1923
PfoI T'CCnGG_A
Cuts at: 0 1142 3785 4056
Size: 1142 2643 271
Fragments arranged by size:
2643 1142 271
PmlI CAC'GTG
Cuts at: 0 1607 3837 4056
Size: 1607 2230 219
Fragments arranged by size:
2230 1607 219
PpuMI rG'GwC_Cy
Cuts at: 0 1259 1616 2789 4056
Size: 1259 357 1173 1267
Fragments arranged by size:
1267 1259 1173 357
PspOMI G'GGCC_C
Cuts at: 0 1010 4056
Size: 1010 3046
PstI C_TGCA'G
Cuts at: 0 782 1478 2007 4056
Size: 782 696 529 2049
Fragments arranged by size:
2049 782 696 529
PvuII CAG'CTG
Cuts at: 0 417 682 912 993 1154 1276 2257 4056
Size: 417 265 230 81 161 122 981 1799
Fragments arranged by size:
1799 981 417 265 230 161 122 81
SacI G_AGCT'C
Cuts at: 0 712 736 1175 1370 4056
Size: 712 24 439 195 2686
Fragments arranged by size:
2686 712 439 195 24
SapI GCTCTTCn'nnn_
Cuts at: 0 2642 3054 4056
Size: 2642 412 1002
Fragments arranged by size:
2642 1002 412
SexAI A'CCwGG_T
Cuts at: 0 1595 4056
Size: 1595 2461
SfcI C'TryA_G
Cuts at: 0 778 969 1277 1474 2003 2153 2333 2408
Size: 778 191 308 197 529 150 180 75
Cuts at: 2408 2532 4056
Size: 124 1524
Fragments arranged by size:
1524 778 529 308 197 191 180 150
124 75
SmaI CCC'GGG
Cuts at: 0 1009 1942 4056
Size: 1009 933 2114
Fragments arranged by size:
2114 1009 933
SmlI C'TyrA_G
Cuts at: 0 1846 2438 2751 4056
Size: 1846 592 313 1305
Fragments arranged by size:
1846 1305 592 313
SphI G_CATG'C
Cuts at: 0 3278 4056
Size: 3278 778
SspI AAT'ATT
Cuts at: 0 2295 3162 4056
Size: 2295 867 894
Fragments arranged by size:
2295 894 867
StuI AGG'CCT
Cuts at: 0 1162 4056
Size: 1162 2894
StyI C'CwwG_G
Cuts at: 0 648 701 3401 4003 4056
Size: 648 53 2700 602 53
Fragments arranged by size:
2700 648 602 53 53
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1198 1453 1751 2140 4051 4056
Size: 1198 255 298 389 1911 5
Fragments arranged by size:
1911 1198 389 298 255 5
TatI w'GTAC_w
Cuts at: 0 1419 2899 2914 4056
Size: 1419 1480 15 1142
Fragments arranged by size:
1480 1419 1142 15
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 422 454 1237 1269 4056
Size: 422 32 783 32 2787
Fragments arranged by size:
2787 783 422 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1234 2199 4056
Size: 1234 965 1857
Fragments arranged by size:
1857 1234 965
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 471 637 699 1572 2881 3399 4056
Size: 471 166 62 873 1309 518 657
Fragments arranged by size:
1309 873 657 518 471 166 62
XmaI C'CCGG_G
Cuts at: 0 1007 1940 4056
Size: 1007 933 2116
Fragments arranged by size:
2116 1007 933
ZraI GAC'GTC
Cuts at: 0 4022 4056
Size: 4022 34
Enzymes that do cut:
AarI AatII AclI AcuI AflII AflIII AleI AlwNI
ApaI ApaLI AseI AvaI AvrII BaeI BanI BanII
BbsI BbvCI BciVI BfrBI BglI BglII Bme1580I BmgBI
BmrI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaHI
BsaXI BseRI BseYI BsgI BsiHKAI BsmI BsmBI Bsp1286I
BspHI BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstEII
BstXI BstYI Bsu36I BtgI BtgZI BtsI CspCI DraIII
DrdI EaeI EarI EciI EcoICRI Eco57MI EcoO109I FalI
Hin4I HincII HindIII HpaI MmeI MscI MslI MspA1I
NcoI NsiI NspI PflMI PfoI PmlI PpuMI PspOMI
PstI PvuII SacI SapI SexAI SfcI SmaI SmlI
SphI SspI StuI StyI TaqII TatI TstI Tth111I
XcmI XmaI ZraI
Enzymes that do not cut:
AccI Acc65I AfeI AgeI AhdI AlfI AloI ApoI
AscI AsiSI BamHI BbeI BcgI BclI BlpI BmtI
BsaBI BsaWI BsiEI BsiWI BspEI BsrBI BssHII BstBI
BstZ17I ClaI DraI EagI EcoNI EcoRI EcoRV FseI
FspI FspAI HaeII KasI KpnI MfeI MluI NaeI
NarI NdeI NgoMIV NheI NotI NruI PacI PasI
PciI PmeI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SacII SalI SanDI SbfI ScaI SfiI SfoI
SgfI SgrAI SnaBI SpeI SrfI SwaI XbaI XhoI
XmnI