(Circular) (Six-base) MAPSORT of: pFN21ASDA0168.seq Check: 9360 from: 1 to: 5707 pFN21ASDA0168 5707 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3517 278 Size: 53 83 186 936 1981 2468 Fragments arranged by size: 2468 1981 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3050 1937 Size: 1113 4594 Acc65I G'GTAC_C Cuts at: 3028 3028 Size: 5707 AclI AA'CG_TT Cuts at: 1563 3834 4207 1563 Size: 2271 373 3063 Fragments arranged by size: 3063 2271 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2123 2339 2432 2657 2747 3770 5154 5422 Size: 491 216 93 225 90 1023 1384 268 Cuts at: 5422 1632 Size: 1917 Fragments arranged by size: 1917 1384 1023 491 268 225 216 93 90 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5510 AflIII A'CryG_T Cuts at: 1203 4606 5583 1203 Size: 3403 977 1327 Fragments arranged by size: 3403 1327 977 AgeI A'CCGG_T Cuts at: 4538 4538 Size: 5707 AhdI GACnn_n'nnGTC Cuts at: 4436 4436 Size: 5707 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5707 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5707 AlwNI CAG_nnn'CTG Cuts at: 2635 2725 2968 3450 5022 2635 Size: 90 243 482 1572 3320 Fragments arranged by size: 3320 1572 482 243 90 ApaI G_GGCC'C Cuts at: 2771 2771 Size: 5707 ApaLI G'TGCA_C Cuts at: 3764 4920 3764 Size: 1156 4551 ApoI r'AATT_y Cuts at: 1493 2134 2654 2668 3016 3242 3394 5503 1493 Size: 641 520 14 348 226 152 2109 1697 Fragments arranged by size: 2109 1697 641 520 348 226 152 14 AseI AT'TA_AT Cuts at: 160 1032 4261 160 Size: 872 3229 1606 Fragments arranged by size: 3229 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5707 AvaI C'yCGr_G Cuts at: 1870 1943 3032 1870 Size: 73 1089 4545 Fragments arranged by size: 4545 1089 73 BamHI G'GATC_C Cuts at: 3037 3404 3037 Size: 367 5340 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2148 3028 4483 618 Size: 325 217 627 361 880 1455 1842 Fragments arranged by size: 1842 1455 880 627 361 325 217 BanII G_rGCy'C Cuts at: 1391 2187 2305 2771 3026 1391 Size: 796 118 466 255 4072 Fragments arranged by size: 4072 796 466 255 118 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2111 2169 928 Size: 971 212 58 4466 Fragments arranged by size: 4466 971 212 58 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3898 3932 1903 Size: 34 1961 34 3678 Fragments arranged by size: 3678 1961 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3599 4809 3599 Size: 1210 4497 BclI T'GATC_A Cuts at: 1548 1548 Size: 5707 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4318 136 Size: 107 122 71 631 605 2646 1525 Fragments arranged by size: 2646 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2346 2781 5702 2346 Size: 435 2921 2351 Fragments arranged by size: 2921 2351 435 BlpI GC'TnA_GC Cuts at: 3124 3124 Size: 5707 Bme1580I G_kGCm'C Cuts at: 1790 2337 2771 3768 4924 1790 Size: 547 434 997 1156 2573 Fragments arranged by size: 2573 1156 997 547 434 BmgBI CAC'GTC Cuts at: 1318 2836 1318 Size: 1518 4189 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2836 3438 4396 Size: 416 53 83 399 24 1419 602 958 Cuts at: 4396 442 Size: 1753 Fragments arranged by size: 1753 1419 958 602 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5707 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2800 2800 Size: 5707 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2386 2747 2876 2990 4367 1239 Size: 1147 361 129 114 1377 2579 Fragments arranged by size: 2579 1377 1147 361 129 114 Bpu10I CC'TnA_GC Cuts at: 2304 2304 Size: 5707 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3832 4697 4995 3832 Size: 865 298 4544 Fragments arranged by size: 4544 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2978 4370 882 Size: 617 1479 1392 2219 Fragments arranged by size: 2219 1479 1392 617 BsaAI yAC'GTr Cuts at: 493 1204 2695 3004 3498 493 Size: 711 1491 309 494 2702 Fragments arranged by size: 2702 1491 711 494 309 BsaBI GATnn'nnATC Cuts at: 5701 5701 Size: 5707 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2492 2518 3514 3896 Size: 53 83 186 936 959 26 996 382 Cuts at: 3896 4531 275 Size: 635 1451 Fragments arranged by size: 1451 996 959 936 635 382 186 83 53 26 BsaWI w'CCGG_w Cuts at: 4140 4538 4812 4959 5370 4140 Size: 398 274 147 411 4477 Fragments arranged by size: 4477 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2513 2543 1178 Size: 30 165 30 1110 30 4342 Fragments arranged by size: 4342 1110 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2260 2263 2693 2987 1186 Size: 1074 3 430 294 3906 Fragments arranged by size: 3906 1074 430 294 3 BseYI C'CCAG_C Cuts at: 2989 4910 5406 2989 Size: 1921 496 3290 Fragments arranged by size: 3290 1921 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5134 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3157 3918 4946 1540 Size: 275 186 1156 761 1028 2301 Fragments arranged by size: 2301 1156 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2946 3768 3853 4924 2946 Size: 822 85 1071 3729 Fragments arranged by size: 3729 1071 822 85 BsmI GAATG_Cn' Cuts at: 2250 3218 3311 2250 Size: 968 93 4646 Fragments arranged by size: 4646 968 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2187 2305 2337 2771 2946 3026 3768 Size: 399 397 118 32 434 175 80 742 Cuts at: 3768 3853 4924 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 434 399 397 175 118 85 80 32 BspEI T'CCGG_A Cuts at: 5370 5370 Size: 5707 BspHI T'CATG_A Cuts at: 3596 5326 3596 Size: 1730 3977 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3062 844 Size: 2218 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3594 1394 Size: 429 1771 3507 Fragments arranged by size: 3507 1771 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2488 4202 4376 65 Size: 1183 1240 1714 174 1396 Fragments arranged by size: 1714 1396 1240 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2211 2863 4351 4538 1700 Size: 110 401 652 1488 187 2869 Fragments arranged by size: 2869 1488 652 401 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5707 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5707 BssSI C'ACGA_G Cuts at: 3767 4779 3767 Size: 1012 4695 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5707 BstBI TT'CG_AA Cuts at: 2007 4511 2007 Size: 2504 3203 BstEII G'GTnAC_C Cuts at: 1184 2375 1184 Size: 1191 4516 BstXI CCAn_nnnn'nTGG Cuts at: 2708 2708 Size: 5707 BstYI r'GATC_y Cuts at: 1972 2346 2781 3037 3404 3789 3806 5258 5344 Size: 374 435 256 367 385 17 1452 86 Cuts at: 5344 5356 5702 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 435 385 374 367 346 256 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2476 513 Size: 552 957 454 3744 Fragments arranged by size: 3744 957 552 454 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4968 Fragments arranged by size: 4968 683 56 BtsI GCAGTG_nn' Cuts at: 2461 3223 4017 4037 2461 Size: 762 794 20 4131 Fragments arranged by size: 4131 794 762 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5707 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5672 DraI TTT'AAA Cuts at: 3011 3011 Size: 5707 DraIII CAC_nnn'GTG Cuts at: 2456 2456 Size: 5707 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4714 809 Size: 507 3398 1802 Fragments arranged by size: 3398 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2539 3154 4043 Size: 54 1425 50 275 18 709 615 889 Cuts at: 4043 5672 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 709 615 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3154 1537 Size: 275 1342 4090 Fragments arranged by size: 4090 1342 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3637 1353 Size: 2284 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4294 4668 4814 387 Size: 1064 138 2705 374 146 1280 Fragments arranged by size: 2705 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2123 2339 2386 2432 2657 2747 2876 Size: 393 491 216 47 46 225 90 129 Cuts at: 2876 2990 3770 4367 5154 5422 1239 Size: 114 780 597 787 268 1524 Fragments arranged by size: 1524 787 780 597 491 393 268 225 216 129 114 90 47 46 EcoNI CCTnn'n_nnAGG Cuts at: 2423 2423 Size: 5707 EcoO109I rG'GnC_Cy Cuts at: 2203 2407 2632 2767 2203 Size: 204 225 135 5143 Fragments arranged by size: 5143 225 204 135 EcoRI G'AATT_C Cuts at: 2668 3016 3394 2668 Size: 348 378 4981 Fragments arranged by size: 4981 378 348 FspI TGC'GCA Cuts at: 2447 2930 4213 2447 Size: 483 1283 3941 Fragments arranged by size: 3941 1283 483 FspAI rTGC'GCAy Cuts at: 2447 2447 Size: 5707 HaeII r_GCGC'y Cuts at: 1678 4854 1678 Size: 3176 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4348 4380 4422 4454 1373 Size: 32 135 32 2776 32 42 32 2626 Fragments arranged by size: 2776 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3051 3297 669 Size: 988 281 1113 246 3079 Fragments arranged by size: 3079 1113 988 281 246 HindIII A'AGCT_T Cuts at: 748 2875 748 Size: 2127 3580 HpaI GTT'AAC Cuts at: 3297 3297 Size: 5707 KpnI G_GTAC'C Cuts at: 3032 3032 Size: 5707 MfeI C'AATT_G Cuts at: 3306 3306 Size: 5707 MluI A'CGCG_T Cuts at: 5583 5583 Size: 5707 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4821 5005 1305 Size: 3516 184 2007 Fragments arranged by size: 3516 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3885 Fragments arranged by size: 3885 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2706 3665 4024 4183 518 Size: 648 1540 959 359 159 2042 Fragments arranged by size: 2042 1540 959 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2440 2506 2746 3123 3800 4948 5193 1606 Size: 834 66 240 377 677 1148 245 2120 Fragments arranged by size: 2120 1148 834 677 377 245 240 66 NaeI GCC'GGC Cuts at: 2213 2213 Size: 5707 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4198 Fragments arranged by size: 4198 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5707 NgoMIV G'CCGG_C Cuts at: 2211 2211 Size: 5707 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5707 NotI GC'GGCC_GC Cuts at: 3154 3154 Size: 5707 NspI r_CATG'y Cuts at: 1132 1229 3065 4610 1132 Size: 97 1836 1545 2229 Fragments arranged by size: 2229 1836 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5707 PciI A'CATG_T Cuts at: 4606 4606 Size: 5707 PflMI CCAn_nnn'nTGG Cuts at: 1108 2582 1108 Size: 1474 4233 PfoI T'CCnGG_A Cuts at: 2892 2892 Size: 5707 PmeI GTTT'AAAC Cuts at: 3011 3011 Size: 5707 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3776 3808 5289 5321 3776 Size: 32 1481 32 4162 Fragments arranged by size: 4162 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2632 2632 Size: 5707 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5707 PsiI TTA'TAA Cuts at: 3277 3277 Size: 5707 PspOMI G'GGCC_C Cuts at: 2767 2767 Size: 5707 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2837 2869 2837 Size: 32 5675 PstI C_TGCA'G Cuts at: 830 2229 2424 3059 830 Size: 1399 195 635 3478 Fragments arranged by size: 3478 1399 635 195 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5707 PvuII CAG'CTG Cuts at: 2440 2440 Size: 5707 SalI G'TCGA_C Cuts at: 1936 3049 1936 Size: 1113 4594 SbfI CC_TGCA'GG Cuts at: 2424 3059 2424 Size: 635 5072 ScaI AGT'ACT Cuts at: 3955 5667 3955 Size: 1712 3995 SexAI A'CCwGG_T Cuts at: 2811 2811 Size: 5707 SfcI C'TryA_G Cuts at: 826 1045 2029 2225 2420 3055 4190 4871 5062 Size: 219 984 196 195 635 1135 681 191 Cuts at: 5062 826 Size: 1471 Fragments arranged by size: 1471 1135 984 681 635 219 196 195 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5707 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5707 SmaI CCC'GGG Cuts at: 1872 3034 1872 Size: 1162 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3811 4712 4974 820 Size: 197 926 1868 901 262 1553 Fragments arranged by size: 1868 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3004 3498 493 Size: 2511 494 2702 Fragments arranged by size: 2702 2511 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5707 SphI G_CATG'C Cuts at: 1132 3065 1132 Size: 1933 3774 SspI AAT'ATT Cuts at: 5 52 3631 5 Size: 47 3579 2081 Fragments arranged by size: 3579 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5707 StyI C'CwwG_G Cuts at: 513 1065 2022 3146 513 Size: 552 957 1124 3074 Fragments arranged by size: 3074 1124 957 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3734 3751 3904 4089 4534 325 Size: 1255 2154 17 153 185 445 1498 Fragments arranged by size: 2154 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2118 2934 3953 Size: 274 80 33 51 1443 141 816 1019 Cuts at: 3953 5665 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1019 816 274 141 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2027 2059 2482 2513 2514 2545 5667 5699 2027 Size: 32 423 31 1 31 3122 32 2035 Fragments arranged by size: 3122 2035 423 32 32 31 31 1 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5707 XbaI T'CTAG_A Cuts at: 3043 3043 Size: 5707 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5707 XmaI C'CCGG_G Cuts at: 1870 3032 1870 Size: 1162 4545 XmnI GAAnn'nnTTC Cuts at: 2250 3836 2250 Size: 1586 4121 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3515 276 Size: 53 83 186 936 1981 2468 Fragments arranged by size: 2468 1981 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI FspAI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI KasI NarI NruI NsiI PacI PmlI PspXI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI XcmI