(Circular) (Six-base) MAPSORT of: pFN21ASDA0157.seq Check: 11 from: 1 to: 5974 pFN21ASDA0157 5974 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3784 278 Size: 53 83 186 936 2248 2468 Fragments arranged by size: 2468 2248 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3317 1937 Size: 1380 4594 Acc65I G'GTAC_C Cuts at: 3295 3295 Size: 5974 AclI AA'CG_TT Cuts at: 1563 2132 4101 4474 1563 Size: 569 1969 373 3063 Fragments arranged by size: 3063 1969 569 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2035 2395 2449 2535 2654 2913 3089 4037 Size: 403 360 54 86 119 259 176 948 Cuts at: 4037 5421 5689 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1384 948 403 360 268 259 176 119 86 54 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5777 AflIII A'CryG_T Cuts at: 1203 4873 5850 1203 Size: 3670 977 1327 Fragments arranged by size: 3670 1327 977 AgeI A'CCGG_T Cuts at: 4805 4805 Size: 5974 AhdI GACnn_n'nnGTC Cuts at: 4703 4703 Size: 5974 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5974 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2846 1184 Size: 1662 4312 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2840 2872 2840 Size: 32 5942 AlwNI CAG_nnn'CTG Cuts at: 3717 5289 3717 Size: 1572 4402 ApaLI G'TGCA_C Cuts at: 4031 5187 4031 Size: 1156 4818 ApoI r'AATT_y Cuts at: 1493 2168 3157 3283 3509 3661 5770 1493 Size: 675 989 126 226 152 2109 1697 Fragments arranged by size: 2109 1697 989 675 226 152 126 AseI AT'TA_AT Cuts at: 160 1032 4528 160 Size: 872 3496 1606 Fragments arranged by size: 3496 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5974 AvaI C'yCGr_G Cuts at: 1870 1943 3299 1870 Size: 73 1356 4545 Fragments arranged by size: 4545 1356 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3056 3089 3056 Size: 33 5941 BamHI G'GATC_C Cuts at: 2929 3304 3671 2929 Size: 375 367 5232 Fragments arranged by size: 5232 375 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3295 4750 618 Size: 325 217 627 1508 1455 1842 Fragments arranged by size: 1842 1508 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3038 3293 1391 Size: 1647 255 4072 Fragments arranged by size: 4072 1647 255 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2541 2764 928 Size: 971 642 223 4138 Fragments arranged by size: 4138 971 642 223 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2907 2941 4165 4199 1903 Size: 34 970 34 1224 34 3678 Fragments arranged by size: 3678 1224 970 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3084 3866 5076 3084 Size: 782 1210 3982 Fragments arranged by size: 3982 1210 782 BclI T'GATC_A Cuts at: 1548 1548 Size: 5974 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4585 136 Size: 107 122 71 631 605 2913 1525 Fragments arranged by size: 2913 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5969 5969 Size: 5974 BlpI GC'TnA_GC Cuts at: 3391 3391 Size: 5974 Bme1580I G_kGCm'C Cuts at: 1790 2391 4035 5191 1790 Size: 601 1644 1156 2573 Fragments arranged by size: 2573 1644 1156 601 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5974 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2117 3705 4663 Size: 416 53 83 399 24 700 1588 958 Cuts at: 4663 442 Size: 1753 Fragments arranged by size: 1753 1588 958 700 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5974 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3006 3006 Size: 5974 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3138 4634 1239 Size: 1899 1496 2579 Fragments arranged by size: 2579 1899 1496 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3009 4099 4964 5262 3009 Size: 1090 865 298 3721 Fragments arranged by size: 3721 1090 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4637 882 Size: 617 3138 2219 Fragments arranged by size: 3138 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3271 3765 493 Size: 711 2067 494 2702 Fragments arranged by size: 2702 2067 711 494 BsaBI GATnn'nnATC Cuts at: 5968 5968 Size: 5974 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2030 3781 4163 4798 Size: 53 83 186 936 497 1751 382 635 Cuts at: 4798 275 Size: 1451 Fragments arranged by size: 1751 1451 936 635 497 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4407 4805 5079 5226 5637 4407 Size: 398 274 147 411 4744 Fragments arranged by size: 4744 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5749 Fragments arranged by size: 5749 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2267 2925 3090 1186 Size: 1081 658 165 4070 Fragments arranged by size: 4070 1081 658 165 BseYI C'CCAG_C Cuts at: 2851 5177 5673 2851 Size: 2326 496 3152 Fragments arranged by size: 3152 2326 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2715 1163 Size: 573 979 4422 Fragments arranged by size: 4422 979 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3424 4185 5213 1540 Size: 275 186 1423 761 1028 2301 Fragments arranged by size: 2301 1423 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2463 4035 4120 5191 2463 Size: 1572 85 1071 3246 Fragments arranged by size: 3246 1572 1071 85 BsmI GAATG_Cn' Cuts at: 3485 3578 3485 Size: 93 5881 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2391 2463 3038 3293 4035 4120 5191 Size: 399 601 72 575 255 742 85 1071 Cuts at: 5191 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 601 575 399 255 85 72 BspEI T'CCGG_A Cuts at: 5637 5637 Size: 5974 BspHI T'CATG_A Cuts at: 3863 5593 3863 Size: 1730 4244 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2341 3329 844 Size: 1497 988 3489 Fragments arranged by size: 3489 1497 988 BsrBI CCG'CTC Cuts at: 1394 1823 3861 1394 Size: 429 2038 3507 Fragments arranged by size: 3507 2038 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4469 4643 65 Size: 1183 3221 174 1396 Fragments arranged by size: 3221 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4618 4805 1700 Size: 110 2808 187 2869 Fragments arranged by size: 2869 2808 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5974 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5974 BssSI C'ACGA_G Cuts at: 4034 5046 4034 Size: 1012 4962 BstAPI GCAn_nnn'nTGC Cuts at: 794 2693 794 Size: 1899 4075 BstBI TT'CG_AA Cuts at: 2007 4778 2007 Size: 2771 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5974 BstYI r'GATC_y Cuts at: 1972 2929 3304 3671 4056 4073 5525 5611 5623 Size: 957 375 367 385 17 1452 86 12 Cuts at: 5623 5969 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 957 385 375 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 3219 513 Size: 552 957 1197 3268 Fragments arranged by size: 3268 1197 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5235 Fragments arranged by size: 5235 683 56 BtsI GCAGTG_nn' Cuts at: 2424 2694 2820 3490 4284 4304 2424 Size: 270 126 670 794 20 4094 Fragments arranged by size: 4094 794 670 270 126 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5974 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5939 DraI TTT'AAA Cuts at: 3278 3278 Size: 5974 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4981 809 Size: 507 3665 1802 Fragments arranged by size: 3665 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3421 4310 5939 Size: 54 1425 50 275 18 1591 889 1629 Cuts at: 5939 8 Size: 43 Fragments arranged by size: 1629 1591 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3421 1537 Size: 275 1609 4090 Fragments arranged by size: 4090 1609 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2414 2468 3043 3904 1353 Size: 1061 54 575 861 3423 Fragments arranged by size: 3423 1061 861 575 54 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4561 4935 5081 387 Size: 1064 138 2972 374 146 1280 Fragments arranged by size: 2972 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2035 2395 2449 2535 2654 2913 3089 Size: 393 403 360 54 86 119 259 176 Cuts at: 3089 3138 4037 4634 5421 5689 1239 Size: 49 899 597 787 268 1524 Fragments arranged by size: 1524 899 787 597 403 393 360 268 259 176 119 86 54 49 EcoO109I rG'GnC_Cy Cuts at: 3238 3238 Size: 5974 EcoRI G'AATT_C Cuts at: 3283 3661 3283 Size: 378 5596 EcoRV GAT'ATC Cuts at: 2496 2496 Size: 5974 FspI TGC'GCA Cuts at: 4480 4480 Size: 5974 HaeII r_GCGC'y Cuts at: 1678 2076 5121 1678 Size: 398 3045 2531 Fragments arranged by size: 3045 2531 398 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4615 4647 4689 4721 1373 Size: 32 135 32 3043 32 42 32 2626 Fragments arranged by size: 3043 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3318 3564 669 Size: 988 281 1380 246 3079 Fragments arranged by size: 3079 1380 988 281 246 HindIII A'AGCT_T Cuts at: 748 2842 748 Size: 2094 3880 HpaI GTT'AAC Cuts at: 3564 3564 Size: 5974 KpnI G_GTAC'C Cuts at: 3299 3299 Size: 5974 MfeI C'AATT_G Cuts at: 3573 3573 Size: 5974 MluI A'CGCG_T Cuts at: 5850 5850 Size: 5974 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5088 5272 1305 Size: 3783 184 2007 Fragments arranged by size: 3783 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4152 Fragments arranged by size: 4152 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3932 4291 4450 518 Size: 648 2766 359 159 2042 Fragments arranged by size: 2766 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2891 3116 3390 4067 5215 5460 1606 Size: 1285 225 274 677 1148 245 2120 Fragments arranged by size: 2120 1285 1148 677 274 245 225 NcoI C'CATG_G Cuts at: 513 1065 2022 3219 513 Size: 552 957 1197 3268 Fragments arranged by size: 3268 1197 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5974 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5974 NotI GC'GGCC_GC Cuts at: 3421 3421 Size: 5974 NspI r_CATG'y Cuts at: 1132 1229 3332 4877 1132 Size: 97 2103 1545 2229 Fragments arranged by size: 2229 2103 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5974 PciI A'CATG_T Cuts at: 4873 4873 Size: 5974 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5974 PfoI T'CCnGG_A Cuts at: 3076 3076 Size: 5974 PmeI GTTT'AAAC Cuts at: 3278 3278 Size: 5974 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2337 2369 4043 4075 5556 5588 2337 Size: 32 1674 32 1481 32 2723 Fragments arranged by size: 2723 1674 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 3238 3238 Size: 5974 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5974 PsiI TTA'TAA Cuts at: 2661 3544 2661 Size: 883 5091 PstI C_TGCA'G Cuts at: 830 3326 830 Size: 2496 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5974 PvuII CAG'CTG Cuts at: 2891 3116 2891 Size: 225 5749 SalI G'TCGA_C Cuts at: 1936 3316 1936 Size: 1380 4594 SapI GCTCTTCn'nnn_ Cuts at: 2468 3043 2468 Size: 575 5399 SbfI CC_TGCA'GG Cuts at: 3326 3326 Size: 5974 ScaI AGT'ACT Cuts at: 2977 4222 5934 2977 Size: 1245 1712 3017 Fragments arranged by size: 3017 1712 1245 SfcI C'TryA_G Cuts at: 826 1045 2341 3171 3322 4457 5138 5329 826 Size: 219 1296 830 151 1135 681 191 1471 Fragments arranged by size: 1471 1296 1135 830 681 219 191 151 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5974 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5974 SmaI CCC'GGG Cuts at: 1872 3301 1872 Size: 1429 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3024 4078 4979 5241 820 Size: 197 926 1081 1054 901 262 1553 Fragments arranged by size: 1553 1081 1054 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3271 3765 493 Size: 2778 494 2702 Fragments arranged by size: 2778 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5974 SphI G_CATG'C Cuts at: 1132 3332 1132 Size: 2200 3774 SspI AAT'ATT Cuts at: 5 52 3898 5 Size: 47 3846 2081 Fragments arranged by size: 3846 2081 47 StuI AGG'CCT Cuts at: 1517 2924 1517 Size: 1407 4567 StyI C'CwwG_G Cuts at: 513 1065 2022 3219 3413 513 Size: 552 957 1197 194 3074 Fragments arranged by size: 3074 1197 957 552 194 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2289 2973 4001 4018 4171 4356 4801 Size: 1255 709 684 1028 17 153 185 445 Cuts at: 4801 325 Size: 1498 Fragments arranged by size: 1498 1255 1028 709 684 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2538 2903 2975 Size: 274 80 33 51 1443 561 365 72 Cuts at: 2975 4220 5932 96 Size: 1245 1712 138 Fragments arranged by size: 1712 1443 1245 561 365 274 138 80 72 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5934 5966 5934 Size: 32 5942 Tth111I GACn'n_nGTC Cuts at: 1316 2396 1316 Size: 1080 4894 XbaI T'CTAG_A Cuts at: 3310 3310 Size: 5974 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3113 3113 Size: 5974 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5974 XmaI C'CCGG_G Cuts at: 1870 3299 1870 Size: 1429 4545 XmnI GAAnn'nnTTC Cuts at: 4103 4103 Size: 5974 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3782 276 Size: 53 83 186 936 2248 2468 Fragments arranged by size: 2468 2248 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI