Restriction map for
pFN21ASDA0155
(Circular) (Six-base) MAPSORT of: pFN21ASDA0155.seq Check: 916 from: 1 to: 8248
pFN21ASDA0155 8248 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4978 6058 278
Size: 53 83 186 936 3442 1080 2468
Fragments arranged by size:
3442 2468 1080 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2372 5591 1937
Size: 435 3219 4594
Fragments arranged by size:
4594 3219 435
Acc65I G'GTAC_C
Cuts at: 5569 5569
Size: 8248
AclI AA'CG_TT
Cuts at: 1563 6375 6748 1563
Size: 4812 373 3063
Fragments arranged by size:
4812 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3329 3657 3680 4194 4265 4370 4384 4728
Size: 1697 328 23 514 71 105 14 344
Cuts at: 4728 6311 7695 7963 1632
Size: 1583 1384 268 1917
Fragments arranged by size:
1917 1697 1583 1384 514 344 328 268
105 71 23 14
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 8051
AflIII A'CryG_T
Cuts at: 1203 3849 4255 7147 8124 1203
Size: 2646 406 2892 977 1327
Fragments arranged by size:
2892 2646 1327 977 406
AgeI A'CCGG_T
Cuts at: 7079 7079
Size: 8248
AhdI GACnn_n'nnGTC
Cuts at: 6977 6977
Size: 8248
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 8248
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 2962 3124 1184
Size: 1778 162 6308
Fragments arranged by size:
6308 1778 162
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2724 2756 2724
Size: 32 8216
AlwNI CAG_nnn'CTG
Cuts at: 2893 5991 7563 2893
Size: 3098 1572 3578
Fragments arranged by size:
3578 3098 1572
ApaI G_GGCC'C
Cuts at: 4201 4660 4201
Size: 459 7789
ApaLI G'TGCA_C
Cuts at: 6305 7461 6305
Size: 1156 7092
ApoI r'AATT_y
Cuts at: 1493 3159 3557 4778 5557 5783 5935 8044 1493
Size: 1666 398 1221 779 226 152 2109 1697
Fragments arranged by size:
2109 1697 1666 1221 779 398 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3702 6802 160
Size: 872 2670 3100 1606
Fragments arranged by size:
3100 2670 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8248
AvaI C'yCGr_G
Cuts at: 1870 1943 3879 4194 4509 5573 1870
Size: 73 1936 315 315 1064 4545
Fragments arranged by size:
4545 1936 1064 315 315 73
AvrII C'CTAG_G
Cuts at: 3421 3631 3421
Size: 210 8038
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 4387 4420 4387
Size: 33 8215
BamHI G'GATC_C
Cuts at: 5486 5578 5945 5486
Size: 92 367 7789
Fragments arranged by size:
7789 367 92
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3308 3395 4261 5569 7024
Size: 325 217 627 1521 87 866 1308 1455
Cuts at: 7024 618
Size: 1842
Fragments arranged by size:
1842 1521 1455 1308 866 627 325 217
87
BanII G_rGCy'C
Cuts at: 1391 2036 2569 2695 4201 4660 5567 1391
Size: 645 533 126 1506 459 907 4072
Fragments arranged by size:
4072 1506 907 645 533 459 126
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 4679 4912 5260 928
Size: 971 2780 233 348 3916
Fragments arranged by size:
3916 2780 971 348 233
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 6439 6473 1903
Size: 34 4502 34 3678
Fragments arranged by size:
4502 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2275 3255 6140 7350 2275
Size: 980 2885 1210 3173
Fragments arranged by size:
3173 2885 1210 980
BclI T'GATC_A
Cuts at: 1548 2401 1548
Size: 853 7395
BfrBI ATG'CAT
Cuts at: 2906 3101 2906
Size: 195 8053
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 6859 136
Size: 107 122 71 631 605 5187 1525
Fragments arranged by size:
5187 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 8243 8243
Size: 8248
BlpI GC'TnA_GC
Cuts at: 5665 5665
Size: 8248
Bme1580I G_kGCm'C
Cuts at: 1790 3313 3400 4201 4516 4660 6309 7465 1790
Size: 1523 87 801 315 144 1649 1156 2573
Fragments arranged by size:
2573 1649 1523 1156 801 315 144 87
BmgBI CAC'GTC
Cuts at: 1318 3519 1318
Size: 2201 6047
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4260 4489 4654
Size: 416 53 83 399 24 2843 229 165
Cuts at: 4654 5979 6937 442
Size: 1325 958 1753
Fragments arranged by size:
2843 1753 1325 958 416 399 229 165
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 2960 3839 1056
Size: 1904 879 5465
Fragments arranged by size:
5465 1904 879
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5398 5398
Size: 8248
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 3357 4400 6908 1239
Size: 2118 1043 2508 2579
Fragments arranged by size:
2579 2508 2118 1043
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2462 6373 7238 7536 2462
Size: 3911 865 298 3174
Fragments arranged by size:
3911 3174 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2244 6911 882
Size: 617 745 4667 2219
Fragments arranged by size:
4667 2219 745 617
BsaAI yAC'GTr
Cuts at: 493 1204 2225 5545 6039 493
Size: 711 1021 3320 494 2702
Fragments arranged by size:
3320 2702 1021 711 494
BsaBI GATnn'nnATC
Cuts at: 8242 8242
Size: 8248
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3449 4975 6055 6437
Size: 53 83 186 936 1916 1526 1080 382
Cuts at: 6437 7072 275
Size: 635 1451
Fragments arranged by size:
1916 1526 1451 1080 936 635 382 186
83 53
BsaWI w'CCGG_w
Cuts at: 2095 5312 6681 7079 7353 7500 7911 2095
Size: 3217 1369 398 274 147 411 2432
Fragments arranged by size:
3217 2432 1369 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2094 2124 2712 2742 1178
Size: 30 165 30 691 30 588 30 6684
Fragments arranged by size:
6684 691 588 165 30 30 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2577 4779 4886 4915 1186
Size: 1391 2202 107 29 4519
Fragments arranged by size:
4519 2202 1391 107 29
BseYI C'CCAG_C
Cuts at: 5389 7451 7947 5389
Size: 2062 496 5690
Fragments arranged by size:
5690 2062 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2129 3377 5363 1163
Size: 573 393 1248 1986 4048
Fragments arranged by size:
4048 1986 1248 573 393
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 5698 6459 7487 1540
Size: 275 186 3697 761 1028 2301
Fragments arranged by size:
3697 2301 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 2569 3048 3919 6309 6394 7465 2569
Size: 479 871 2390 85 1071 3352
Fragments arranged by size:
3352 2390 1071 871 479 85
BsmI GAATG_Cn'
Cuts at: 2681 2906 4232 5759 5852 2681
Size: 225 1326 1527 93 5077
Fragments arranged by size:
5077 1527 1326 225 93
BsmBI CGTCTCn'nnnn_
Cuts at: 4619 4619
Size: 8248
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2036 2569 2695 3048 3313 3400 3919
Size: 399 246 533 126 353 265 87 519
Cuts at: 3919 4201 4516 4660 5567 6309 6394 7465 1391
Size: 282 315 144 907 742 85 1071 2174
Fragments arranged by size:
2174 1071 907 742 533 519 399 353
315 282 265 246 144 126 87 85
BspEI T'CCGG_A
Cuts at: 7911 7911
Size: 8248
BspHI T'CATG_A
Cuts at: 6137 7867 6137
Size: 1730 6518
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 5603 844
Size: 4759 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4537 6135 1394
Size: 429 2714 1598 3507
Fragments arranged by size:
3507 2714 1598 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2588 2638 6743 6917 65
Size: 1183 1340 50 4105 174 1396
Fragments arranged by size:
4105 1396 1340 1183 174 50
BsrFI r'CCGG_y
Cuts at: 1700 1810 5231 6892 7079 1700
Size: 110 3421 1661 187 2869
Fragments arranged by size:
3421 2869 1661 187 110
BsrGI T'GTAC_A
Cuts at: 96 2302 96
Size: 2206 6042
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 8248
BssSI C'ACGA_G
Cuts at: 4020 6308 7320 4020
Size: 2288 1012 4948
Fragments arranged by size:
4948 2288 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 8248
BstBI TT'CG_AA
Cuts at: 2007 7052 2007
Size: 5045 3203
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 8248
BstXI CCAn_nnnn'nTGG
Cuts at: 2716 3723 5462 2716
Size: 1007 1739 5502
Fragments arranged by size:
5502 1739 1007
BstYI r'GATC_y
Cuts at: 1972 2344 4928 5432 5486 5578 5945 6330 6347
Size: 372 2584 504 54 92 367 385 17
Cuts at: 6347 7799 7885 7897 8243 1972
Size: 1452 86 12 346 1977
Fragments arranged by size:
2584 1977 1452 504 385 372 367 346
92 86 54 17 12
BstZ17I GTA'TAC
Cuts at: 2373 2373
Size: 8248
BtgI C'CryG_G
Cuts at: 513 1065 3639 513
Size: 552 2574 5122
Fragments arranged by size:
5122 2574 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3563 487
Size: 683 56 2337 5172
Fragments arranged by size:
5172 2337 683 56
BtsI GCAGTG_nn'
Cuts at: 4475 4855 5764 6558 6578 4475
Size: 380 909 794 20 6145
Fragments arranged by size:
6145 909 794 380 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 8248
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3007 3042 529
Size: 35 2443 35 5735
Fragments arranged by size:
5735 2443 35 35
DraI TTT'AAA
Cuts at: 5552 5552
Size: 8248
DraIII CAC_nnn'GTG
Cuts at: 3818 4268 3818
Size: 450 7798
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 7255 809
Size: 507 5939 1802
Fragments arranged by size:
5939 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2379 3921 5452
Size: 54 1425 50 275 18 549 1542 1531
Cuts at: 5452 5695 6584 8213 8
Size: 243 889 1629 43
Fragments arranged by size:
1629 1542 1531 1425 889 549 275 243
54 50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5695 1537
Size: 275 3883 4090
Fragments arranged by size:
4090 3883 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2190 2416 3699 4208 4527 4599 4623 4823
Size: 837 226 1283 509 319 72 24 200
Cuts at: 4823 5086 6178 1353
Size: 263 1092 3423
Fragments arranged by size:
3423 1283 1092 837 509 319 263 226
200 72 24
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 6835 7209 7355 387
Size: 1064 138 5246 374 146 1280
Fragments arranged by size:
5246 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2567 2567
Size: 8248
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 3329 3357 3657 3680 4194 4265 4370
Size: 393 1697 28 300 23 514 71 105
Cuts at: 4370 4384 4400 4728 6311 6908 7695 7963 1239
Size: 14 16 328 1583 597 787 268 1524
Fragments arranged by size:
1697 1583 1524 787 597 514 393 328
300 268 105 71 28 23 16 14
EcoNI CCTnn'n_nnAGG
Cuts at: 2441 3425 2441
Size: 984 7264
EcoO109I rG'GnC_Cy
Cuts at: 3767 4198 5307 3767
Size: 431 1109 6708
Fragments arranged by size:
6708 1109 431
EcoRI G'AATT_C
Cuts at: 3557 5557 5935 3557
Size: 2000 378 5870
Fragments arranged by size:
5870 2000 378
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3676 3676
Size: 8248
FspI TGC'GCA
Cuts at: 6754 6754
Size: 8248
HaeII r_GCGC'y
Cuts at: 1678 2168 4122 7395 1678
Size: 490 1954 3273 2531
Fragments arranged by size:
3273 2531 1954 490
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2935 2967 3710 3724 3742
Size: 32 135 32 1363 32 743 14 18
Cuts at: 3742 3756 4894 4926 5258 5276 5290 5308 5321
Size: 14 1138 32 332 18 14 18 13
Cuts at: 5321 5353 5405 5417 5437 5449 5498 5530 6889
Size: 32 52 12 20 12 49 32 1359
Cuts at: 6889 6921 6963 6995 1373
Size: 32 42 32 2626
Fragments arranged by size:
2626 1363 1359 1138 743 332 135 52
49 42 32 32 32 32 32 32
32 32 20 18 18 18 14 14
14 13 12 12
HincII GTy'rAC
Cuts at: 669 1657 1938 3616 5592 5838 669
Size: 988 281 1678 1976 246 3079
Fragments arranged by size:
3079 1976 1678 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2529 2994 3129 3261 748
Size: 1781 465 135 132 5735
Fragments arranged by size:
5735 1781 465 135 132
HpaI GTT'AAC
Cuts at: 5838 5838
Size: 8248
KpnI G_GTAC'C
Cuts at: 5573 5573
Size: 8248
MfeI C'AATT_G
Cuts at: 5847 5847
Size: 8248
MluI A'CGCG_T
Cuts at: 8124 8124
Size: 8248
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2193 2710 5514 7362 7546 1305
Size: 888 517 2804 1848 184 2007
Fragments arranged by size:
2804 2007 1848 888 517 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 3923 5454 10
Size: 54 1425 343 2091 1531 2804
Fragments arranged by size:
2804 2091 1531 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3106 3717 4092 4094 6206 6565 6724
Size: 648 1940 611 375 2 2112 359 159
Cuts at: 6724 518
Size: 2042
Fragments arranged by size:
2112 2042 1940 648 611 375 359 159
2
MspA1I CmG'CkG
Cuts at: 1606 2615 4654 5260 5664 6341 7489 7734 1606
Size: 1009 2039 606 404 677 1148 245 2120
Fragments arranged by size:
2120 2039 1148 1009 677 606 404 245
NaeI GCC'GGC
Cuts at: 5233 5233
Size: 8248
NcoI C'CATG_G
Cuts at: 513 1065 3639 513
Size: 552 2574 5122
Fragments arranged by size:
5122 2574 552
NdeI CA'TA_TG
Cuts at: 387 2150 387
Size: 1763 6485
NgoMIV G'CCGG_C
Cuts at: 5231 5231
Size: 8248
NheI G'CTAG_C
Cuts at: 1052 2956 3835 1052
Size: 1904 879 5465
Fragments arranged by size:
5465 1904 879
NotI GC'GGCC_GC
Cuts at: 5695 5695
Size: 8248
NsiI A_TGCA'T
Cuts at: 2908 3103 2908
Size: 195 8053
NspI r_CATG'y
Cuts at: 1132 1229 4259 5606 7151 1132
Size: 97 3030 1347 1545 2229
Fragments arranged by size:
3030 2229 1545 1347 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 8248
PciI A'CATG_T
Cuts at: 4255 7147 4255
Size: 2892 5356
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2416 2893 3056 4666 1108
Size: 1308 477 163 1610 4690
Fragments arranged by size:
4690 1610 1308 477 163
PfoI T'CCnGG_A
Cuts at: 2052 2052
Size: 8248
PmeI GTTT'AAAC
Cuts at: 5552 5552
Size: 8248
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 6317 6349 7830 7862 6317
Size: 32 1481 32 6703
Fragments arranged by size:
6703 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 3767 3767
Size: 8248
PshAI GACnn'nnGTC
Cuts at: 1613 5260 1613
Size: 3647 4601
PsiI TTA'TAA
Cuts at: 5818 5818
Size: 8248
PspOMI G'GGCC_C
Cuts at: 4197 4656 4197
Size: 459 7789
PstI C_TGCA'G
Cuts at: 830 5600 830
Size: 4770 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 8248
SacI G_AGCT'C
Cuts at: 2569 2569
Size: 8248
SalI G'TCGA_C
Cuts at: 1936 5590 1936
Size: 3654 4594
SapI GCTCTTCn'nnn_
Cuts at: 2416 3699 4527 4623 2416
Size: 1283 828 96 6041
Fragments arranged by size:
6041 1283 828 96
SbfI CC_TGCA'GG
Cuts at: 5600 5600
Size: 8248
ScaI AGT'ACT
Cuts at: 3005 3939 4359 6496 8208 3005
Size: 934 420 2137 1712 3045
Fragments arranged by size:
3045 2137 1712 934 420
SfcI C'TryA_G
Cuts at: 826 1045 2245 4455 5596 6731 7412 7603 826
Size: 219 1200 2210 1141 1135 681 191 1471
Fragments arranged by size:
2210 1471 1200 1141 1135 681 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 8248
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 8248
SmaI CCC'GGG
Cuts at: 1872 4196 4511 5575 1872
Size: 2324 315 1064 4545
Fragments arranged by size:
4545 2324 1064 315
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2441 6352 7253 7515 820
Size: 197 926 498 3911 901 262 1553
Fragments arranged by size:
3911 1553 926 901 498 262 197
SnaBI TAC'GTA
Cuts at: 493 5545 6039 493
Size: 5052 494 2702
Fragments arranged by size:
5052 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 8248
SphI G_CATG'C
Cuts at: 1132 5606 1132
Size: 4474 3774
SrfI GCCC'GGGC
Cuts at: 4196 4511 4196
Size: 315 7933
SspI AAT'ATT
Cuts at: 5 52 2509 3440 4684 6172 5
Size: 47 2457 931 1244 1488 2081
Fragments arranged by size:
2457 2081 1488 1244 931 47
StuI AGG'CCT
Cuts at: 1517 5501 1517
Size: 3984 4264
StyI C'CwwG_G
Cuts at: 513 1065 3213 3421 3631 3639 3841 4296 5455
Size: 552 2148 208 210 8 202 455 1159
Cuts at: 5455 5687 513
Size: 232 3074
Fragments arranged by size:
3074 2148 1159 552 455 232 210 208
202 8
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3383 4279 6275 6292 6445 6630 7075
Size: 1255 1803 896 1996 17 153 185 445
Cuts at: 7075 325
Size: 1498
Fragments arranged by size:
1996 1803 1498 1255 896 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 2302 2485 3003
Size: 274 80 33 51 1443 325 183 518
Cuts at: 3003 3937 4110 4180 4357 6494 8206 96
Size: 934 173 70 177 2137 1712 138
Fragments arranged by size:
2137 1712 1443 934 518 325 274 183
177 173 138 80 70 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 8208 8240 8208
Size: 32 8216
Tth111I GACn'n_nGTC
Cuts at: 1316 2066 1316
Size: 750 7498
XbaI T'CTAG_A
Cuts at: 2743 5584 2743
Size: 2841 5407
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 3410 5216 3410
Size: 1806 6442
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 8248
XmaI C'CCGG_G
Cuts at: 1870 4194 4509 5573 1870
Size: 2324 315 1064 4545
Fragments arranged by size:
4545 2324 1064 315
XmnI GAAnn'nnTTC
Cuts at: 3693 6377 3693
Size: 2684 5564
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4976 6056 276
Size: 53 83 186 936 3442 1080 2468
Fragments arranged by size:
3442 2468 1080 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI AvrII BaeI BamHI BanI BanII
BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI
Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI
BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI
BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII
Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI
MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI
NotI NsiI NspI PasI PciI PflMI PfoI PmeI
PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI
SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SrfI SspI StuI StyI
TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AarI AfeI AscI BbeI BbvCI Bpu10I BsiWI Bsu36I
EcoRV FseI FspAI KasI NarI NruI PacI PmlI
PspXI PsrI PvuII RsrII SacII SanDI SexAI SfiI
SfoI SwaI