Restriction map for
pFN21ASDA0154
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# NO CUT ENZYMES ( 98 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AgeI Alw44I Aor51HI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspI AviII AvrII
BciVI BfrI BglI BglII BlnI
BsaBI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI BspEI BspHI
BspLU11I BsrDI BsrFI BssSI Bst1107I
BstAPI BstBI BstZ17I Cfr10I CpoI
DraIII DrdI EagI EclXI Eco47III
Eco52I EcoRI EcoRV EcoT22I FseI
FspI KpnI KspI MamI MfeI
MluI MroI MunI NaeI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsrII SacII SalI ScaI
SexAI SfiI SfuI SgrAI SnaBI
SpeI SphI SspI SwaI Tth111I
XcmI XhoI XmnI
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# ONE CUT ENZYMES ( 47 enzymes) #
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AccII 1434 CGCG CG^CG
AflIII 548 ACACGT A^C[GA][CT]GT
BamHI 521 GGATCC G^GATCC
BbeI 1219 GGCGCC GGCGC^C
BbrPI 1654 CACGTG CAC^GTG
BbvCI 1247 CCTCAGC CCTCAGC(-5/-2)
BcgI 872 GCACGCTCATCG (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BlpI 2010 GCTGAGC GC^T[ACGT]AGC
Bpu1102I 2010 GCTGAGC GC^T[ACGT]AGC
BsaAI 1654 CACGTG [CT]AC^GT[GA]
Bsp1407I 254 TGTACA T^GTACA
BspDI 1668 ATCGAT AT^CGAT
BsrGI 254 TGTACA T^GTACA
BssHII 1433 GCGCGC G^CGCGC
BstEII 531 GGTGACC G^GT[ACGT]ACC
BstPI 531 GGTGACC G^GT[ACGT]ACC
BstUI 1434 CGCG CG^CG
BstXI 169 CCACAGGAATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CelII 2010 GCTGAGC GC^T[ACGT]AGC
ClaI 1668 ATCGAT AT^CGAT
DraI 688 TTTAAA TTT^AAA
EcoNI 468 CCTGCAGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 531 GGTGACC G^GT[ACGT]ACC
HincII 562 GTTAAC GT[CT]^[GA]AC
HindII 562 GTTAAC GT[CT]^[GA]AC
HindIII 427 AAGCTT A^AGCTT
HpaI 562 GTTAAC GTT^AAC
KasI 1219 GGCGCC G^GCGCC
MvnI 1434 CGCG CG^CG
NarI 1219 GGCGCC GG^CGCC
NspI 308 ACATGC [GA]CATG^[CT]
PmaCI 1654 CACGTG CAC^GTG
PmlI 1654 CACGTG CAC^GTG
PpuMI 990 AGGACCT [GA]G^G[AT]CC[CT]
SacI 1633 GAGCTC GAGCT^C
SapI 1581 GCTCTTC GCTCTTC(1/4)
SbfI 468 CCTGCAGG CCTGCA^GG
SfoI 1219 GGCGCC GGC^GCC
SmaI 761 CCCGGG CCC^GGG
SmlI 1260 CTCAAG C^T[CT][GA]AG
Sse8387I 468 CCTGCAGG CCTGCA^GG
SspBI 254 TGTACA T^GTACA
StuI 983 AGGCCT AGG^CCT
TfiI 1666 GAATC G^A[AT]TC
XbaI 1467 TCTAGA T^CTAGA
XmaCI 761 CCCGGG C^CCGGG
XmaI 761 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 31 enzymes) #
###################################
AcyI 298 GACGCC G[GA]^CG[CT]C
1219 GGCGCC
AfaI 201 GTAC GT^AC
255 GTAC
AhdI 211 GACAGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
534 GACCAAGCGTC
AocI 950 CCTCAGG CC^T[ACGT]AGG
986 CCTCAGG
ApaI 1036 GGGCCC GGGCC^C
1567 GGGCCC
AspEI 211 GACAGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
534 GACCAAGCGTC
AspHI 1633 GAGCTC G[AT]GC[AT]^C
2013 GAGCAC
AvaI 761 CCCGGG C^[CT]CG[GA]G
1488 CTCGGG
BalI 1643 TGGCCA TGG^CCA
1946 TGGCCA
BbsI 231 GAAGAC GAAGAC(2/6)
508 GAAGAC
BclI 99 TGATCA T^GATCA
657 TGATCA
BpuAI 231 GAAGAC GAAGAC(2/6)
508 GAAGAC
BsaHI 298 GACGCC G[GA]^CG[CT]C
1219 GGCGCC
BsiHKAI 1633 GAGCTC G[AT]GC[AT]^C
2013 GAGCAC
BsoBI 761 CCCGGG C^[CT]CG[GA]G
1488 CTCGGG
BsrBI 1005 CCGCTC CCGCTC(-3/-3)
1579 CCGCTC
BstYI 158 AGATCC [GA]^GATC[CT]
521 GGATCC
Bsu36I 950 CCTCAGG CC^T[ACGT]AGG
986 CCTCAGG
EaeI 1643 TGGCCA [CT]^GGCC[GA]
1946 TGGCCA
Eam1105I 211 GACAGGGTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
534 GACCAAGCGTC
Eco81I 950 CCTCAGG CC^T[ACGT]AGG
986 CCTCAGG
FbaI 99 TGATCA T^GATCA
657 TGATCA
HaeII 183 GGCGCT [GA]GCGC^[CT]
1219 GGCGCC
Hin1I 298 GACGCC G[GA]^CG[CT]C
1219 GGCGCC
MflI 158 AGATCC [GA]^GATC[CT]
521 GGATCC
MluNI 1643 TGGCCA TGG^CCA
1946 TGGCCA
MscI 1643 TGGCCA TGG^CCA
1946 TGGCCA
PflMI 1410 CCATGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1873 CCACCTTCTGG
RsaI 201 GTAC GT^AC
255 GTAC
Van91I 1410 CCATGGCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1873 CCACCTTCTGG
XhoII 158 AGATCC [GA]^GATC[CT]
521 GGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 82 enzymes) #
#########################################
AciI AluI AlwI AlwNI AvaII
BanI BanII BbvI BcnI BfaI
BmyI BpmI BsaJI BseRI BsgI
BsiYI BslI BsmAI BsmFI Bsp1286I
BspMI BsrI BssKI BstF5I BstNI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DsaI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeI
MaeII MaeIII MboI MboII MnlI
MseI MslI MspA1I MspI MvaI
MwoI NciI NcoI NdeII NlaIII
NlaIV PleI PstI PvuII Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI Tru9I TseI Tsp45I Tsp509I
TspRI TthHB8I