(Circular) (Six-base) MAPSORT of: pFN21ASDA0154.seq Check: 6893 from: 1 to: 6799 pFN21ASDA0154 6799 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3186 3949 3186 Size: 763 6036 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4609 278 Size: 53 83 186 936 3073 2468 Fragments arranged by size: 3073 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4142 1937 Size: 2205 4594 Acc65I G'GTAC_C Cuts at: 4120 4120 Size: 6799 AclI AA'CG_TT Cuts at: 1563 4926 5299 1563 Size: 3363 373 3063 Fragments arranged by size: 3363 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2357 3196 4001 4862 6246 6514 1632 Size: 725 839 805 861 1384 268 1917 Fragments arranged by size: 1917 1384 861 839 805 725 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6602 AflIII A'CryG_T Cuts at: 1203 2571 5698 6675 1203 Size: 1368 3127 977 1327 Fragments arranged by size: 3127 1368 1327 977 AgeI A'CCGG_T Cuts at: 5630 5630 Size: 6799 AhdI GACnn_n'nnGTC Cuts at: 2239 2562 5528 2239 Size: 323 2966 3510 Fragments arranged by size: 3510 2966 323 AleI CACnn'nnGTG Cuts at: 1166 4043 1166 Size: 2877 3922 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3695 3869 1184 Size: 2511 174 4114 Fragments arranged by size: 4114 2511 174 AlwNI CAG_nnn'CTG Cuts at: 2218 3326 3425 3616 4542 6114 2218 Size: 1108 99 191 926 1572 2903 Fragments arranged by size: 2903 1572 1108 926 191 99 ApaI G_GGCC'C Cuts at: 3063 3594 3063 Size: 531 6268 ApaLI G'TGCA_C Cuts at: 4856 6012 4856 Size: 1156 5643 ApoI r'AATT_y Cuts at: 1493 4108 4334 4486 6595 1493 Size: 2615 226 152 2109 1697 Fragments arranged by size: 2615 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5353 160 Size: 872 4321 1606 Fragments arranged by size: 4321 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6799 AvaI C'yCGr_G Cuts at: 1870 1943 2784 3511 4124 1870 Size: 73 841 727 613 4545 Fragments arranged by size: 4545 841 727 613 73 BamHI G'GATC_C Cuts at: 2544 4129 4496 2544 Size: 1585 367 4847 Fragments arranged by size: 4847 1585 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3174 3242 3429 4120 5575 Size: 325 217 627 1387 68 187 691 1455 Cuts at: 5575 618 Size: 1842 Fragments arranged by size: 1842 1455 1387 691 627 325 217 187 68 BanII G_rGCy'C Cuts at: 1391 3063 3365 3417 3594 3660 4118 1391 Size: 1672 302 52 177 66 458 4072 Fragments arranged by size: 4072 1672 458 302 177 66 52 BbeI G_GCGC'C Cuts at: 3246 3246 Size: 6799 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2261 2538 928 Size: 971 362 277 5189 Fragments arranged by size: 5189 971 362 277 BbvCI CC'TCA_GC Cuts at: 3271 3271 Size: 6799 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2884 2918 4990 5024 1903 Size: 34 947 34 2072 34 3678 Fragments arranged by size: 3678 2072 947 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4691 5901 4691 Size: 1210 5589 BclI T'GATC_A Cuts at: 1548 2122 2680 1548 Size: 574 558 5667 Fragments arranged by size: 5667 574 558 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5410 136 Size: 107 122 71 631 605 3738 1525 Fragments arranged by size: 3738 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6794 6794 Size: 6799 BlpI GC'TnA_GC Cuts at: 4034 4216 4034 Size: 182 6617 Bme1580I G_kGCm'C Cuts at: 1790 3063 3432 3594 3684 3712 3870 4860 6016 Size: 1273 369 162 90 28 158 990 1156 Cuts at: 6016 1790 Size: 2573 Fragments arranged by size: 2573 1273 1156 990 369 162 158 90 28 BmgBI CAC'GTC Cuts at: 1318 3793 1318 Size: 2475 4324 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2107 3348 4530 Size: 416 53 83 399 24 690 1241 1182 Cuts at: 4530 5488 442 Size: 958 1753 Fragments arranged by size: 1753 1241 1182 958 690 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6799 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2069 2195 3167 3248 3402 5459 1239 Size: 830 126 972 81 154 2057 2579 Fragments arranged by size: 2579 2057 972 830 154 126 81 Bpu10I CC'TnA_GC Cuts at: 3271 3654 3271 Size: 383 6416 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3268 4924 5789 6087 3268 Size: 1656 865 298 3980 Fragments arranged by size: 3980 1656 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5462 882 Size: 617 3963 2219 Fragments arranged by size: 3963 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3679 4096 4590 493 Size: 711 2475 417 494 2702 Fragments arranged by size: 2702 2475 711 494 417 BsaBI GATnn'nnATC Cuts at: 6793 6793 Size: 6799 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2322 3243 4606 4988 Size: 53 83 186 936 789 921 1363 382 Cuts at: 4988 5623 275 Size: 635 1451 Fragments arranged by size: 1451 1363 936 921 789 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5232 5630 5904 6051 6462 5232 Size: 398 274 147 411 5569 Fragments arranged by size: 5569 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2765 2795 3068 3098 1178 Size: 30 165 30 1362 30 273 30 4879 Fragments arranged by size: 4879 1362 273 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2453 2962 3022 3113 3246 3271 3355 1186 Size: 1267 509 60 91 133 25 84 4630 Fragments arranged by size: 4630 1267 509 133 91 84 60 25 BseYI C'CCAG_C Cuts at: 2953 3062 3127 3158 3319 3334 3391 3559 6002 Size: 109 65 31 161 15 57 168 2443 Cuts at: 6002 6498 2953 Size: 496 3254 Fragments arranged by size: 3254 2443 496 168 161 109 65 57 31 15 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2375 2552 2902 3446 1163 Size: 573 639 177 350 544 4516 Fragments arranged by size: 4516 639 573 544 350 177 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4249 5010 6038 1540 Size: 275 186 2248 761 1028 2301 Fragments arranged by size: 2301 2248 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3660 4040 4860 4945 6016 3660 Size: 380 820 85 1071 4443 Fragments arranged by size: 4443 1071 820 380 85 BsmI GAATG_Cn' Cuts at: 4310 4403 4310 Size: 93 6706 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3063 3365 3417 3432 3594 3660 3684 Size: 399 1273 302 52 15 162 66 24 Cuts at: 3684 3712 3870 4040 4118 4860 4945 6016 1391 Size: 28 158 170 78 742 85 1071 2174 Fragments arranged by size: 2174 1273 1071 742 399 302 170 162 158 85 78 66 52 28 24 15 BspEI T'CCGG_A Cuts at: 6462 6462 Size: 6799 BspHI T'CATG_A Cuts at: 4688 6418 4688 Size: 1730 5069 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2499 2812 3186 3949 4154 844 Size: 1655 313 374 763 205 3489 Fragments arranged by size: 3489 1655 763 374 313 205 BsrBI CCG'CTC Cuts at: 1394 1823 3030 3604 4686 1394 Size: 429 1207 574 1082 3507 Fragments arranged by size: 3507 1207 1082 574 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5294 5468 65 Size: 1183 4046 174 1396 Fragments arranged by size: 4046 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5443 5630 1700 Size: 110 3633 187 2869 Fragments arranged by size: 3633 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2277 96 Size: 2181 4618 BssHII G'CGCG_C Cuts at: 1925 3456 1925 Size: 1531 5268 BssSI C'ACGA_G Cuts at: 4859 5871 4859 Size: 1012 5787 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6799 BstBI TT'CG_AA Cuts at: 2007 5603 2007 Size: 3596 3203 BstEII G'GTnAC_C Cuts at: 1184 2554 1184 Size: 1370 5429 BstXI CCAn_nnnn'nTGG Cuts at: 2199 2199 Size: 6799 BstYI r'GATC_y Cuts at: 1972 2181 2544 4129 4496 4881 4898 6350 6436 Size: 209 363 1585 367 385 17 1452 86 Cuts at: 6436 6448 6794 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1585 1452 385 367 363 346 209 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2974 3010 2974 Size: 36 6763 BtgI C'CryG_G Cuts at: 513 1065 2022 2289 2520 3020 3433 3585 513 Size: 552 957 267 231 500 413 152 3727 Fragments arranged by size: 3727 957 552 500 413 267 231 152 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6060 Fragments arranged by size: 6060 683 56 BtsI GCAGTG_nn' Cuts at: 3302 3712 3870 4315 5109 5129 3302 Size: 410 158 445 794 20 4972 Fragments arranged by size: 4972 794 445 410 158 20 ClaI AT'CG_AT Cuts at: 1554 3692 1554 Size: 2138 4661 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2358 2393 529 Size: 35 1794 35 4935 Fragments arranged by size: 4935 1794 35 35 DraI TTT'AAA Cuts at: 2713 4103 2713 Size: 1390 5409 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5806 809 Size: 507 4490 1802 Fragments arranged by size: 4490 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3666 3969 4246 Size: 54 1425 50 275 18 1836 303 277 Cuts at: 4246 5135 6764 8 Size: 889 1629 43 Fragments arranged by size: 1836 1629 1425 889 303 277 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4246 1537 Size: 275 2434 4090 Fragments arranged by size: 4090 2434 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2333 2647 3489 3611 3758 4729 1353 Size: 980 314 842 122 147 971 3423 Fragments arranged by size: 3423 980 971 842 314 147 122 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2042 2048 2716 5386 5760 5906 Size: 1064 138 453 6 668 2670 374 146 Cuts at: 5906 387 Size: 1280 Fragments arranged by size: 2670 1280 1064 668 453 374 146 138 6 EcoICRI GAG'CTC Cuts at: 3658 3658 Size: 6799 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2069 2195 2357 3167 3196 3248 3402 Size: 393 437 126 162 810 29 52 154 Cuts at: 3402 4001 4862 5459 6246 6514 1239 Size: 599 861 597 787 268 1524 Fragments arranged by size: 1524 861 810 787 599 597 437 393 268 162 154 126 52 29 EcoNI CCTnn'n_nnAGG Cuts at: 2495 2495 Size: 6799 EcoO109I rG'GnC_Cy Cuts at: 2215 3014 3059 3567 3590 3650 2215 Size: 799 45 508 23 60 5364 Fragments arranged by size: 5364 799 508 60 45 23 EcoRI G'AATT_C Cuts at: 4108 4486 4108 Size: 378 6421 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4030 4030 Size: 6799 FspI TGC'GCA Cuts at: 5305 5305 Size: 6799 HaeII r_GCGC'y Cuts at: 1678 2210 3246 5946 1678 Size: 532 1036 2700 2531 Fragments arranged by size: 2700 2531 1036 532 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2225 2257 2383 2415 2429 Size: 32 135 32 653 32 126 32 14 Cuts at: 2429 2461 2548 2580 2765 2797 5440 5472 5514 Size: 32 87 32 185 32 2643 32 42 Cuts at: 5514 5546 1373 Size: 32 2626 Fragments arranged by size: 2643 2626 653 185 135 126 87 42 32 32 32 32 32 32 32 32 32 14 HincII GTy'rAC Cuts at: 669 1657 1938 2587 4143 4389 669 Size: 988 281 649 1556 246 3079 Fragments arranged by size: 3079 1556 988 649 281 246 HindIII A'AGCT_T Cuts at: 748 2450 748 Size: 1702 5097 HpaI GTT'AAC Cuts at: 2587 4389 2587 Size: 1802 4997 KasI G'GCGC_C Cuts at: 3242 3242 Size: 6799 KpnI G_GTAC'C Cuts at: 4124 4124 Size: 6799 MfeI C'AATT_G Cuts at: 4398 4398 Size: 6799 MluI A'CGCG_T Cuts at: 6675 6675 Size: 6799 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2106 2188 3222 3273 5913 6097 1305 Size: 801 82 1034 51 2640 184 2007 Fragments arranged by size: 2640 2007 1034 801 184 82 51 MscI TGG'CCA Cuts at: 10 64 1489 1832 3668 3971 10 Size: 54 1425 343 1836 303 2838 Fragments arranged by size: 2838 1836 1425 343 303 54 MslI CAynn'nnrTG Cuts at: 518 1166 2197 3471 3677 4043 4757 5116 5275 Size: 648 1031 1274 206 366 714 359 159 Cuts at: 5275 518 Size: 2042 Fragments arranged by size: 2042 1274 1031 714 648 366 359 206 159 MspA1I CmG'CkG Cuts at: 1606 2283 3158 3233 4033 4215 4892 6040 6285 Size: 677 875 75 800 182 677 1148 245 Cuts at: 6285 1606 Size: 2120 Fragments arranged by size: 2120 1148 875 800 677 677 245 182 75 NarI GG'CG_CC Cuts at: 3243 3243 Size: 6799 NcoI C'CATG_G Cuts at: 513 1065 2022 2289 2520 3433 513 Size: 552 957 267 231 913 3879 Fragments arranged by size: 3879 957 913 552 267 231 NdeI CA'TA_TG Cuts at: 387 387 Size: 6799 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6799 NotI GC'GGCC_GC Cuts at: 4246 4246 Size: 6799 NspI r_CATG'y Cuts at: 1132 1229 2335 4157 5702 1132 Size: 97 1106 1822 1545 2229 Fragments arranged by size: 2229 1822 1545 1106 97 PasI CC'CwG_GG Cuts at: 1348 3055 3425 3631 4021 1348 Size: 1707 370 206 390 4126 Fragments arranged by size: 4126 1707 390 370 206 PciI A'CATG_T Cuts at: 5698 5698 Size: 6799 PflMI CCAn_nnn'nTGG Cuts at: 1108 3439 3902 1108 Size: 2331 463 4005 Fragments arranged by size: 4005 2331 463 PmeI GTTT'AAAC Cuts at: 4103 4103 Size: 6799 PmlI CAC'GTG Cuts at: 3679 3679 Size: 6799 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4868 4900 6381 6413 4868 Size: 32 1481 32 5254 Fragments arranged by size: 5254 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3014 3014 Size: 6799 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6799 PsiI TTA'TAA Cuts at: 4369 4369 Size: 6799 PspOMI G'GGCC_C Cuts at: 3059 3590 3059 Size: 531 6268 PstI C_TGCA'G Cuts at: 830 2496 3858 3865 3894 3946 4151 830 Size: 1666 1362 7 29 52 205 3478 Fragments arranged by size: 3478 1666 1362 205 52 29 7 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6799 PvuII CAG'CTG Cuts at: 2283 3158 4033 2283 Size: 875 875 5049 Fragments arranged by size: 5049 875 875 SacI G_AGCT'C Cuts at: 3660 3660 Size: 6799 SalI G'TCGA_C Cuts at: 1936 4141 1936 Size: 2205 4594 SapI GCTCTTCn'nnn_ Cuts at: 3611 3611 Size: 6799 SbfI CC_TGCA'GG Cuts at: 2496 4151 2496 Size: 1655 5144 ScaI AGT'ACT Cuts at: 5047 6759 5047 Size: 1712 5087 SfcI C'TryA_G Cuts at: 826 1045 2492 3854 3861 3890 3942 4147 5282 Size: 219 1447 1362 7 29 52 205 1135 Cuts at: 5282 5963 6154 826 Size: 681 191 1471 Fragments arranged by size: 1471 1447 1362 1135 681 219 205 191 52 29 7 SfoI GGC'GCC Cuts at: 3244 3244 Size: 6799 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6799 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6799 SmaI CCC'GGG Cuts at: 1872 2786 4126 1872 Size: 914 1340 4545 Fragments arranged by size: 4545 1340 914 SmlI C'TyrA_G Cuts at: 820 1017 1943 3283 4903 5804 6066 820 Size: 197 926 1340 1620 901 262 1553 Fragments arranged by size: 1620 1553 1340 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4096 4590 493 Size: 3603 494 2702 Fragments arranged by size: 3603 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6799 SphI G_CATG'C Cuts at: 1132 4157 1132 Size: 3025 3774 SspI AAT'ATT Cuts at: 5 52 4723 5 Size: 47 4671 2081 Fragments arranged by size: 4671 2081 47 StuI AGG'CCT Cuts at: 1517 3008 1517 Size: 1491 5308 StyI C'CwwG_G Cuts at: 513 1065 2022 2259 2289 2520 2890 3396 3433 Size: 552 957 237 30 231 370 506 37 Cuts at: 3433 3765 4238 513 Size: 332 473 3074 Fragments arranged by size: 3074 957 552 506 473 370 332 237 231 37 30 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3968 4826 4843 4996 5181 5626 325 Size: 1255 2388 858 17 153 185 445 1498 Fragments arranged by size: 2388 1498 1255 858 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2277 5045 6757 Size: 274 80 33 51 1443 300 2768 1712 Cuts at: 6757 96 Size: 138 Fragments arranged by size: 2768 1712 1443 300 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3606 3638 6759 6791 3606 Size: 32 3121 32 3614 Fragments arranged by size: 3614 3121 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6799 XbaI T'CTAG_A Cuts at: 3490 4135 3490 Size: 645 6154 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6799 XmaI C'CCGG_G Cuts at: 1870 2784 4124 1870 Size: 914 1340 4545 Fragments arranged by size: 4545 1340 914 XmnI GAAnn'nnTTC Cuts at: 4928 4928 Size: 6799 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4607 276 Size: 53 83 186 936 3073 2468 Fragments arranged by size: 3073 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PspXI PsrI RsrII SacII SanDI SexAI SfiI SrfI SwaI XcmI