Restriction map for
pFN21ASDA0153
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# NO CUT ENZYMES ( 101 enzymes) #
##################################
AflII AflIII AgeI AhdI Aor51HI
AscI AseI AsnI AspEI AspI
AvrII BbeI BbrPI BcgI BciVI
BclI BfaI BfrI BlnI BlpI
Bpu1102I BsaAI BsaBI BsaI BsiEI
BsiWI BsmI Bsp1407I BspDI BspLU11I
BsrDI BsrGI Bst1107I BstBI BstZ17I
CelII ClaI CpoI DraI DraIII
EagI Eam1105I EclXI Eco47III Eco52I
EcoNI EcoRI EcoRV EcoT22I FbaI
FseI HindIII HinfI HpaI KasI
KspI MaeI MamI MfeI MluI
MseI MunI NarI NdeI NheI
NotI NruI NsiI NspV PacI
PaeR7I PflFI PflMI PinAI PleI
PmaCI PmeI PmlI PshBI PvuI
RsrII SacI SacII SapI ScaI
SexAI SfoI SfuI SgrAI SmlI
SnaBI SpeI SspBI SspI SwaI
TfiI Tru9I Tth111I Van91I XbaI
XhoI
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# ONE CUT ENZYMES ( 42 enzymes) #
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Acc65I 1850 GGTACC G^GTACC
AccIII 752 TCCGGA T^CCGGA
AclI 137 AACGTT AA^CGTT
AcsI 1621 GAATTT [GA]^AATT[CT]
AocI 684 CCTGAGG CC^T[ACGT]AGG
ApoI 1621 GAATTT [GA]^AATT[CT]
Asp718I 1850 GGTACC G^GTACC
AviII 1178 TGCGCA TGC^GCA
BamHI 599 GGATCC G^GATCC
BglI 1139 GCCCCGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 1649 AGATCT A^GATCT
BsaWI 752 TCCGGA [AT]^CCGG[AT]
BseAI 752 TCCGGA T^CCGGA
BsmBI 736 GAGACG CGTCTC(1/5)
BspEI 752 TCCGGA T^CCGGA
BspHI 1541 TCATGA T^CATGA
BssSI 172 CACGAG CACGAG(-5/-1)
BstAPI 405 GCAGCAGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 711 GGTGACC G^GT[ACGT]ACC
BstPI 711 GGTGACC G^GT[ACGT]ACC
Bsu36I 684 CCTGAGG CC^T[ACGT]AGG
DrdI 1282 GACACCCACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 684 CCTGAGG CC^T[ACGT]AGG
EcoO65I 711 GGTGACC G^GT[ACGT]ACC
FspI 1178 TGCGCA TGC^GCA
HincII 1747 GTCGAC GT[CT]^[GA]AC
HindII 1747 GTCGAC GT[CT]^[GA]AC
KpnI 1850 GGTACC GGTAC^C
MroI 752 TCCGGA T^CCGGA
NaeI 26 GCCGGC GCC^GGC
NcoI 540 CCATGG C^CATGG
NgoMIV 26 GCCGGC G^CCGGC
PshAI 1632 GACGGACGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
Psp1406I 137 AACGTT AA^CGTT
RcaI 1541 TCATGA T^CATGA
SalI 1747 GTCGAC G^TCGAC
SbfI 1844 CCTGCAGG CCTGCA^GG
SfiI 1138 GGCCCCGAGGGCC GGCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGCC
SphI 761 GCATGC GCATG^C
Sse8387I 1844 CCTGCAGG CCTGCA^GG
StuI 77 AGGCCT AGG^CCT
Tsp509I 1622 AATT ^AATT
###################################
# TWO CUT ENZYMES ( 19 enzymes) #
###################################
AatII 1636 GACGTC GACGT^C
1873 GACGTC
AccI 919 GTCTAC GT^[AC][GT]AC
1747 GTCGAC
Alw44I 892 GTGCAC G^TGCAC
1576 GTGCAC
ApaI 776 GGGCCC GGGCC^C
1146 GGGCCC
ApaLI 892 GTGCAC G^TGCAC
1576 GTGCAC
Asp700I 1068 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1527 GAAGCACTTC
BalI 434 TGGCCA TGG^CCA
941 TGGCCA
BbsI 1384 GAAGAC GAAGAC(2/6)
1876 GTCTTC
BbvCI 798 CCTCAGC CCTCAGC(-5/-2)
550 GCTGAGG
BpuAI 1384 GAAGAC GAAGAC(2/6)
1876 GTCTTC
BsmAI 719 GAGAC GTCTC(1/5)
736 GAGAC
BsrBI 10 GAGCGG CCGCTC(-3/-3)
318 GAGCGG
BsrFI 26 GCCGGC [GA]^CCGG[CT]
564 GCCGGT
BstXI 437 CCAACCTGATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1899 CCAGCCCGGTGG
Cfr10I 26 GCCGGC [GA]^CCGG[CT]
564 GCCGGT
MluNI 434 TGGCCA TGG^CCA
941 TGGCCA
MscI 434 TGGCCA TGG^CCA
941 TGGCCA
PstI 403 CTGCAG CTGCA^G
1845 CTGCAG
XmnI 1068 GAACCAGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
1527 GAAGCACTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 96 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI AlwNI AspHI AvaI AvaII
BanI BanII BbvI BcnI BmyI
BpmI BsaHI BsaJI BseRI BsgI
BsiHKAI BsiYI BslI BsmFI BsoBI
Bsp1286I BspMI BsrI BssHII BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DdeI DpnII DraII
DsaI EaeI EarI EcoO109I EcoRII
EcoT14I FauI Fnu4HI FokI HaeII
HaeIII HapII HgaI HhaI Hin1I
HinP1I HpaII HphI ItaI Ksp632I
MaeII MaeIII MboI MboII MflI
MnlI MslI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PpuMI PvuII RsaI
Sau3AI Sau96I ScrFI SfaNI SfcI
SmaI StyI TaqI TseI Tsp45I
TspRI TthHB8I XcmI XhoII XmaCI
XmaI