(Circular) (Six-base) MAPSORT of: pFN21ASDA0153.seq Check: 3696 from: 1 to: 6661 pFN21ASDA0153 6661 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2422 2671 2950 3146 3586 2422 Size: 249 279 196 440 5497 Fragments arranged by size: 5497 440 279 249 196 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3663 3900 4471 278 Size: 53 83 186 936 2127 237 571 2468 Fragments arranged by size: 2468 2127 936 571 237 186 83 53 AccI GT'mk_AC Cuts at: 1937 2943 3771 3954 4004 1937 Size: 1006 828 183 50 4594 Fragments arranged by size: 4594 1006 828 183 50 Acc65I G'GTAC_C Cuts at: 3873 3982 3873 Size: 109 6552 AclI AA'CG_TT Cuts at: 1563 2161 4788 5161 1563 Size: 598 2627 373 3063 Fragments arranged by size: 3063 2627 598 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3611 3803 3886 4724 6108 6376 1632 Size: 1979 192 83 838 1384 268 1917 Fragments arranged by size: 1979 1917 1384 838 268 192 83 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6464 AflIII A'CryG_T Cuts at: 1203 5560 6537 1203 Size: 4357 977 1327 Fragments arranged by size: 4357 1327 977 AgeI A'CCGG_T Cuts at: 5492 5492 Size: 6661 AhdI GACnn_n'nnGTC Cuts at: 5390 5390 Size: 6661 AleI CACnn'nnGTG Cuts at: 1166 2951 1166 Size: 1785 4876 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2807 3227 1184 Size: 1623 420 4618 Fragments arranged by size: 4618 1623 420 AlwNI CAG_nnn'CTG Cuts at: 2425 2507 3832 4404 5976 2425 Size: 82 1325 572 1572 3110 Fragments arranged by size: 3110 1572 1325 572 82 ApaI G_GGCC'C Cuts at: 2803 3173 2803 Size: 370 6291 ApaLI G'TGCA_C Cuts at: 2915 3599 4718 5874 2915 Size: 684 1119 1156 3702 Fragments arranged by size: 3702 1156 1119 684 ApoI r'AATT_y Cuts at: 1493 3644 3970 4196 4348 6457 1493 Size: 2151 326 226 152 2109 1697 Fragments arranged by size: 2151 2109 1697 326 226 152 AseI AT'TA_AT Cuts at: 160 1032 5215 160 Size: 872 4183 1606 Fragments arranged by size: 4183 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6661 AvaI C'yCGr_G Cuts at: 1870 1943 2042 2071 2271 2299 2438 2739 2837 Size: 73 99 29 200 28 139 301 98 Cuts at: 2837 3164 3620 3753 3986 1870 Size: 327 456 133 233 4545 Fragments arranged by size: 4545 456 327 301 233 200 139 133 99 98 73 29 28 BamHI G'GATC_C Cuts at: 2622 3991 4358 2622 Size: 1369 367 4925 Fragments arranged by size: 4925 1369 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2434 2656 3342 3873 3982 Size: 325 217 627 647 222 686 531 109 Cuts at: 3982 5437 618 Size: 1455 1842 Fragments arranged by size: 1842 1455 686 647 627 531 325 222 217 109 BanII G_rGCy'C Cuts at: 1391 2349 2803 3173 3980 1391 Size: 958 454 370 807 4072 Fragments arranged by size: 4072 958 807 454 370 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3414 3892 928 Size: 971 1515 478 3697 Fragments arranged by size: 3697 1515 971 478 BbvCI CC'TCA_GC Cuts at: 2574 2822 2574 Size: 248 6413 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4852 4886 1903 Size: 34 2915 34 3678 Fragments arranged by size: 3678 2915 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4553 5763 4553 Size: 1210 5451 BclI T'GATC_A Cuts at: 1548 1548 Size: 6661 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3168 5272 136 Size: 107 122 71 631 605 1496 2104 1525 Fragments arranged by size: 2104 1525 1496 631 605 122 107 71 BglII A'GATC_T Cuts at: 3672 6656 3672 Size: 2984 3677 BlpI GC'TnA_GC Cuts at: 4078 4078 Size: 6661 Bme1580I G_kGCm'C Cuts at: 1790 2439 2643 2803 2919 3173 3603 4722 5878 Size: 649 204 160 116 254 430 1119 1156 Cuts at: 5878 1790 Size: 2573 Fragments arranged by size: 2573 1156 1119 649 430 254 204 160 116 BmgBI CAC'GTC Cuts at: 1318 2397 2638 2953 3313 3412 1318 Size: 1079 241 315 360 99 4567 Fragments arranged by size: 4567 1079 360 315 241 99 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2176 2490 3211 Size: 416 53 83 399 24 759 314 721 Cuts at: 3211 4392 5350 442 Size: 1181 958 1753 Fragments arranged by size: 1753 1181 958 759 721 416 399 314 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6661 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2210 2333 2543 2891 3854 5321 1239 Size: 971 123 210 348 963 1467 2579 Fragments arranged by size: 2579 1467 971 963 348 210 123 Bpu10I CC'TnA_GC Cuts at: 2574 2822 2574 Size: 248 6413 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4786 5651 5949 4786 Size: 865 298 5498 Fragments arranged by size: 5498 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5324 882 Size: 617 3825 2219 Fragments arranged by size: 3825 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3958 4452 493 Size: 711 2754 494 2702 Fragments arranged by size: 2754 2702 711 494 BsaBI GATnn'nnATC Cuts at: 6655 6655 Size: 6661 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2081 3015 3660 3897 Size: 53 83 186 936 548 934 645 237 Cuts at: 3897 4468 4850 5485 275 Size: 571 382 635 1451 Fragments arranged by size: 1451 936 934 645 635 571 548 382 237 186 83 53 BsaWI w'CCGG_w Cuts at: 2775 5094 5492 5766 5913 6324 2775 Size: 2319 398 274 147 411 3112 Fragments arranged by size: 3112 2319 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2516 2546 1178 Size: 30 165 30 1113 30 5293 Fragments arranged by size: 5293 1113 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2102 2255 2267 2270 2540 2591 2744 1186 Size: 916 153 12 3 270 51 153 5103 Fragments arranged by size: 5103 916 270 153 153 51 12 3 BseYI C'CCAG_C Cuts at: 2210 2642 5864 6360 2210 Size: 432 3222 496 2511 Fragments arranged by size: 3222 2511 496 432 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2108 2165 2432 2975 3311 1163 Size: 573 372 57 267 543 336 4513 Fragments arranged by size: 4513 573 543 372 336 267 57 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4111 4872 5900 1540 Size: 275 186 2110 761 1028 2301 Fragments arranged by size: 2301 2110 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2196 2412 2856 2919 3356 3603 4722 4807 5878 Size: 216 444 63 437 247 1119 85 1071 Cuts at: 5878 2196 Size: 2979 Fragments arranged by size: 2979 1119 1071 444 437 247 216 85 63 BsmI GAATG_Cn' Cuts at: 4172 4265 4172 Size: 93 6568 BsmBI CGTCTCn'nnnn_ Cuts at: 2753 2753 Size: 6661 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2196 2349 2412 2439 2643 2803 2856 Size: 399 406 153 63 27 204 160 53 Cuts at: 2856 2919 3173 3356 3603 3980 4722 4807 5878 Size: 63 254 183 247 377 742 85 1071 Cuts at: 5878 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 406 399 377 254 247 204 183 160 153 85 63 63 53 27 BspEI T'CCGG_A Cuts at: 2775 6324 2775 Size: 3549 3112 BspHI T'CATG_A Cuts at: 3564 4550 6280 3564 Size: 986 1730 3945 Fragments arranged by size: 3945 1730 986 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2422 2671 2950 3120 3146 3207 3586 3861 Size: 1578 249 279 170 26 61 379 275 Cuts at: 3861 3953 4016 844 Size: 92 63 3489 Fragments arranged by size: 3489 1578 379 279 275 249 170 92 63 61 26 BsrBI CCG'CTC Cuts at: 1394 1823 2035 2343 4548 1394 Size: 429 212 308 2205 3507 Fragments arranged by size: 3507 2205 429 308 212 BsrDI GCAATG_nn' Cuts at: 65 1248 5156 5330 65 Size: 1183 3908 174 1396 Fragments arranged by size: 3908 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2049 2587 5305 5492 1700 Size: 110 239 538 2718 187 2869 Fragments arranged by size: 2869 2718 538 239 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6661 BssHII G'CGCG_C Cuts at: 1925 2094 2140 2414 3146 3293 1925 Size: 169 46 274 732 147 5293 Fragments arranged by size: 5293 732 274 169 147 46 BssSI C'ACGA_G Cuts at: 2195 4721 5733 2195 Size: 2526 1012 3123 Fragments arranged by size: 3123 2526 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2434 794 Size: 1640 5021 BstBI TT'CG_AA Cuts at: 2007 5465 2007 Size: 3458 3203 BstEII G'GTnAC_C Cuts at: 1184 2734 1184 Size: 1550 5111 BstXI CCAn_nnnn'nTGG Cuts at: 2467 3929 2467 Size: 1462 5199 BstYI r'GATC_y Cuts at: 1972 2622 3270 3672 3828 3991 4358 4743 4760 Size: 650 648 402 156 163 367 385 17 Cuts at: 4760 6212 6298 6310 6656 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 650 648 402 385 367 346 163 156 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2708 2708 Size: 6661 BtgI C'CryG_G Cuts at: 513 1065 2022 2482 2563 2652 2910 2923 513 Size: 552 957 460 81 89 258 13 4251 Fragments arranged by size: 4251 957 552 460 258 89 81 13 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3127 487 Size: 683 56 1901 4021 Fragments arranged by size: 4021 1901 683 56 BtsI GCAGTG_nn' Cuts at: 4177 4971 4991 4177 Size: 794 20 5847 Fragments arranged by size: 5847 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6661 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6626 DraI TTT'AAA Cuts at: 3965 3965 Size: 6661 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3311 5668 809 Size: 507 1995 2357 1802 Fragments arranged by size: 2357 1995 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2106 2457 2926 Size: 54 1425 50 275 18 276 351 469 Cuts at: 2926 2964 4108 4997 6626 8 Size: 38 1144 889 1629 43 Fragments arranged by size: 1629 1425 1144 889 469 351 276 275 54 50 43 38 18 EagI C'GGCC_G Cuts at: 1537 1812 4108 1537 Size: 275 2296 4090 Fragments arranged by size: 4090 2296 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2244 3032 3603 4591 1353 Size: 891 788 571 988 3423 Fragments arranged by size: 3423 988 891 788 571 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3026 5248 5622 5768 387 Size: 1064 138 1437 2222 374 146 1280 Fragments arranged by size: 2222 1437 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2210 2333 2543 2891 3611 3803 3854 Size: 393 578 123 210 348 720 192 51 Cuts at: 3854 3886 4724 5321 6108 6376 1239 Size: 32 838 597 787 268 1524 Fragments arranged by size: 1524 838 787 720 597 578 393 348 268 210 192 123 51 32 EcoO109I rG'GnC_Cy Cuts at: 2039 2152 2713 2800 3169 3465 2039 Size: 113 561 87 369 296 5235 Fragments arranged by size: 5235 561 369 296 113 87 EcoRI G'AATT_C Cuts at: 3970 4348 3970 Size: 378 6283 FspI TGC'GCA Cuts at: 3203 5167 3203 Size: 1964 4697 HaeII r_GCGC'y Cuts at: 1678 2050 2123 2138 5808 1678 Size: 372 73 15 3670 2531 Fragments arranged by size: 3670 2531 372 73 15 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2810 2842 3731 3763 5302 Size: 32 135 32 1238 32 889 32 1539 Cuts at: 5302 5334 5376 5408 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1539 1238 889 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3772 4005 4251 669 Size: 988 281 1834 233 246 3079 Fragments arranged by size: 3079 1834 988 281 246 233 HindIII A'AGCT_T Cuts at: 748 748 Size: 6661 HpaI GTT'AAC Cuts at: 4251 4251 Size: 6661 KpnI G_GTAC'C Cuts at: 3877 3986 3877 Size: 109 6552 MfeI C'AATT_G Cuts at: 4260 4260 Size: 6661 MluI A'CGCG_T Cuts at: 6537 6537 Size: 6661 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3534 5775 5959 1305 Size: 2229 2241 184 2007 Fragments arranged by size: 2241 2229 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2459 2966 10 Size: 54 1425 343 627 507 3705 Fragments arranged by size: 3705 1425 627 507 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2234 2810 2951 3563 4619 4978 5137 Size: 648 1068 576 141 612 1056 359 159 Cuts at: 5137 518 Size: 2042 Fragments arranged by size: 2042 1068 1056 648 612 576 359 159 141 MspA1I CmG'CkG Cuts at: 1606 2425 2557 2573 3241 4077 4754 5902 6147 Size: 819 132 16 668 836 677 1148 245 Cuts at: 6147 1606 Size: 2120 Fragments arranged by size: 2120 1148 836 819 677 668 245 132 16 NaeI GCC'GGC Cuts at: 2051 2051 Size: 6661 NcoI C'CATG_G Cuts at: 513 1065 2022 2563 513 Size: 552 957 541 4611 Fragments arranged by size: 4611 957 552 541 NdeI CA'TA_TG Cuts at: 387 387 Size: 6661 NgoMIV G'CCGG_C Cuts at: 2049 2049 Size: 6661 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6661 NotI GC'GGCC_GC Cuts at: 4108 4108 Size: 6661 NspI r_CATG'y Cuts at: 1132 1229 2674 2788 2815 4019 5564 1132 Size: 97 1445 114 27 1204 1545 2229 Fragments arranged by size: 2229 1545 1445 1204 114 97 27 PasI CC'CwG_GG Cuts at: 1348 2796 3727 1348 Size: 1448 931 4282 Fragments arranged by size: 4282 1448 931 PciI A'CATG_T Cuts at: 5560 5560 Size: 6661 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6661 PfoI T'CCnGG_A Cuts at: 3831 3831 Size: 6661 PmeI GTTT'AAAC Cuts at: 3965 3965 Size: 6661 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4730 4762 6243 6275 4730 Size: 32 1481 32 5116 Fragments arranged by size: 5116 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2039 2152 2713 2039 Size: 113 561 5987 Fragments arranged by size: 5987 561 113 PshAI GACnn'nnGTC Cuts at: 1613 3659 1613 Size: 2046 4615 PsiI TTA'TAA Cuts at: 4231 4231 Size: 6661 PspOMI G'GGCC_C Cuts at: 2799 3169 2799 Size: 370 6291 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 3476 3508 3476 Size: 32 6629 PstI C_TGCA'G Cuts at: 830 2430 3872 4013 830 Size: 1600 1442 141 3478 Fragments arranged by size: 3478 1600 1442 141 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6661 PvuII CAG'CTG Cuts at: 2425 2557 2573 2425 Size: 132 16 6513 Fragments arranged by size: 6513 132 16 SalI G'TCGA_C Cuts at: 1936 3770 4003 1936 Size: 1834 233 4594 Fragments arranged by size: 4594 1834 233 SanDI GG'GwC_CC Cuts at: 2039 2152 2039 Size: 113 6548 SbfI CC_TGCA'GG Cuts at: 3872 4013 3872 Size: 141 6520 ScaI AGT'ACT Cuts at: 4909 6621 4909 Size: 1712 4949 SfcI C'TryA_G Cuts at: 826 1045 2426 3052 3868 4009 5144 5825 6016 Size: 219 1381 626 816 141 1135 681 191 Cuts at: 6016 826 Size: 1471 Fragments arranged by size: 1471 1381 1135 816 681 626 219 191 141 SfiI GGCCn_nnn'nGGCC Cuts at: 3168 3168 Size: 6661 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6661 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6661 SmaI CCC'GGG Cuts at: 1872 2073 2273 2301 2440 3755 3988 1872 Size: 201 200 28 139 1315 233 4545 Fragments arranged by size: 4545 1315 233 201 200 139 28 SmlI C'TyrA_G Cuts at: 820 1017 1943 4765 5666 5928 820 Size: 197 926 2822 901 262 1553 Fragments arranged by size: 2822 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3958 4452 493 Size: 3465 494 2702 Fragments arranged by size: 3465 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6661 SphI G_CATG'C Cuts at: 1132 2788 4019 1132 Size: 1656 1231 3774 Fragments arranged by size: 3774 1656 1231 SrfI GCCC'GGGC Cuts at: 2073 2440 2073 Size: 367 6294 SspI AAT'ATT Cuts at: 5 52 4585 5 Size: 47 4533 2081 Fragments arranged by size: 4533 2081 47 StuI AGG'CCT Cuts at: 1517 2102 1517 Size: 585 6076 StyI C'CwwG_G Cuts at: 513 1065 2022 2103 2563 3360 4100 513 Size: 552 957 81 460 797 740 3074 Fragments arranged by size: 3074 957 797 740 552 460 81 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2993 3014 3323 4688 4705 4858 5043 Size: 1255 1413 21 309 1365 17 153 185 Cuts at: 5043 5488 325 Size: 445 1498 Fragments arranged by size: 1498 1413 1365 1255 445 309 185 153 21 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2498 3375 4907 Size: 274 80 33 51 1443 521 877 1532 Cuts at: 4907 6619 96 Size: 1712 138 Fragments arranged by size: 1712 1532 1443 877 521 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2386 2418 2516 2548 3798 3830 6621 6653 2386 Size: 32 98 32 1250 32 2791 32 2394 Fragments arranged by size: 2791 2394 1250 98 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6661 XbaI T'CTAG_A Cuts at: 3997 3997 Size: 6661 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2561 2812 3648 2561 Size: 251 836 5574 Fragments arranged by size: 5574 836 251 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6661 XmaI C'CCGG_G Cuts at: 1870 2071 2271 2299 2438 3753 3986 1870 Size: 201 200 28 139 1315 233 4545 Fragments arranged by size: 4545 1315 233 201 200 139 28 XmnI GAAnn'nnTTC Cuts at: 3095 3554 4790 3095 Size: 459 1236 4966 Fragments arranged by size: 4966 1236 459 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3661 3898 4469 276 Size: 53 83 186 936 2127 237 571 2468 Fragments arranged by size: 2468 2127 936 571 237 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PsrI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspXI RsrII SacI SacII SapI SexAI SfoI SwaI