Restriction map for
pFN21ASDA0153
(Linear) (Six-base) MAPSORT of: KIAA0153.seq Check: 564 from: 1 to: 1932
KIAA0153.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 399 648 927 1123 1563 1932
Size: 399 249 279 196 440 369
Fragments arranged by size:
440 399 369 279 249 196
AatII G_ACGT'C
Cuts at: 0 1640 1877 1932
Size: 1640 237 55
Fragments arranged by size:
1640 237 55
AccI GT'mk_AC
Cuts at: 0 920 1748 1932
Size: 920 828 184
Fragments arranged by size:
920 828 184
Acc65I G'GTAC_C
Cuts at: 0 1850 1932
Size: 1850 82
AclI AA'CG_TT
Cuts at: 0 138 1932
Size: 138 1794
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1588 1780 1863 1932
Size: 1588 192 83 69
Fragments arranged by size:
1588 192 83 69
AleI CACnn'nnGTG
Cuts at: 0 928 1932
Size: 928 1004
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 784 1204 1932
Size: 784 420 728
Fragments arranged by size:
784 728 420
AlwNI CAG_nnn'CTG
Cuts at: 0 402 484 1809 1932
Size: 402 82 1325 123
Fragments arranged by size:
1325 402 123 82
ApaI G_GGCC'C
Cuts at: 0 780 1150 1932
Size: 780 370 782
Fragments arranged by size:
782 780 370
ApaLI G'TGCA_C
Cuts at: 0 892 1576 1932
Size: 892 684 356
Fragments arranged by size:
892 684 356
ApoI r'AATT_y
Cuts at: 0 1621 1932
Size: 1621 311
AvaI C'yCGr_G
Cuts at: 0 19 48 248 276 415 716 814 1141
Size: 19 29 200 28 139 301 98 327
Cuts at: 1141 1597 1730 1932
Size: 456 133 202
Fragments arranged by size:
456 327 301 202 200 139 133 98
29 28 19
BamHI G'GATC_C
Cuts at: 0 599 1932
Size: 599 1333
BanI G'GyrC_C
Cuts at: 0 411 633 1319 1850 1932
Size: 411 222 686 531 82
Fragments arranged by size:
686 531 411 222 82
BanII G_rGCy'C
Cuts at: 0 326 780 1150 1932
Size: 326 454 370 782
Fragments arranged by size:
782 454 370 326
BbsI GAAGACnn'nnnn_
Cuts at: 0 1391 1869 1932
Size: 1391 478 63
Fragments arranged by size:
1391 478 63
BbvCI CC'TCA_GC
Cuts at: 0 551 799 1932
Size: 551 248 1133
Fragments arranged by size:
1133 551 248
BglI GCCn_nnn'nGGC
Cuts at: 0 1145 1932
Size: 1145 787
BglII A'GATC_T
Cuts at: 0 1649 1932
Size: 1649 283
Bme1580I G_kGCm'C
Cuts at: 0 416 620 780 896 1150 1580 1932
Size: 416 204 160 116 254 430 352
Fragments arranged by size:
430 416 352 254 204 160 116
BmgBI CAC'GTC
Cuts at: 0 374 615 930 1290 1389 1932
Size: 374 241 315 360 99 543
Fragments arranged by size:
543 374 360 315 241 99
BmrI ACTGGGnnnn_n'
Cuts at: 0 153 467 1188 1932
Size: 153 314 721 744
Fragments arranged by size:
744 721 314 153
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 310 520 868 1831 1932
Size: 187 123 210 348 963 101
Fragments arranged by size:
963 348 210 187 123 101
Bpu10I CC'TnA_GC
Cuts at: 0 551 799 1932
Size: 551 248 1133
Fragments arranged by size:
1133 551 248
BsaHI Gr'CG_yC
Cuts at: 0 58 992 1637 1874 1932
Size: 58 934 645 237 58
Fragments arranged by size:
934 645 237 58 58
BsaWI w'CCGG_w
Cuts at: 0 752 1932
Size: 752 1180
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 493 523 1932
Size: 493 30 1409
Fragments arranged by size:
1409 493 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 79 232 244 247 517 568 721 1932
Size: 79 153 12 3 270 51 153 1211
Fragments arranged by size:
1211 270 153 153 79 51 12 3
BseYI C'CCAG_C
Cuts at: 0 187 619 1932
Size: 187 432 1313
Fragments arranged by size:
1313 432 187
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 142 409 952 1288 1932
Size: 85 57 267 543 336 644
Fragments arranged by size:
644 543 336 267 85 57
BsiHKAI G_wGCw'C
Cuts at: 0 173 389 833 896 1333 1580 1932
Size: 173 216 444 63 437 247 352
Fragments arranged by size:
444 437 352 247 216 173 63
BsmBI CGTCTCn'nnnn_
Cuts at: 0 730 1932
Size: 730 1202
Bsp1286I G_dGCh'C
Cuts at: 0 173 326 389 416 620 780 833 896
Size: 173 153 63 27 204 160 53 63
Cuts at: 896 1150 1333 1580 1932
Size: 254 183 247 352
Fragments arranged by size:
352 254 247 204 183 173 160 153
63 63 53 27
BspEI T'CCGG_A
Cuts at: 0 752 1932
Size: 752 1180
BspHI T'CATG_A
Cuts at: 0 1541 1932
Size: 1541 391
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 399 648 927 1097 1123 1184 1563 1838
Size: 399 249 279 170 26 61 379 275
Cuts at: 1838 1930 1932
Size: 92 2
Fragments arranged by size:
399 379 279 275 249 170 92 61
26 2
BsrBI CCG'CTC
Cuts at: 0 12 320 1932
Size: 12 308 1612
Fragments arranged by size:
1612 308 12
BsrFI r'CCGG_y
Cuts at: 0 26 564 1932
Size: 26 538 1368
Fragments arranged by size:
1368 538 26
BssHII G'CGCG_C
Cuts at: 0 71 117 391 1123 1270 1932
Size: 71 46 274 732 147 662
Fragments arranged by size:
732 662 274 147 71 46
BssSI C'ACGA_G
Cuts at: 0 172 1932
Size: 172 1760
BstAPI GCAn_nnn'nTGC
Cuts at: 0 411 1932
Size: 411 1521
BstEII G'GTnAC_C
Cuts at: 0 711 1932
Size: 711 1221
BstXI CCAn_nnnn'nTGG
Cuts at: 0 444 1906 1932
Size: 444 1462 26
Fragments arranged by size:
1462 444 26
BstYI r'GATC_y
Cuts at: 0 599 1247 1649 1805 1932
Size: 599 648 402 156 127
Fragments arranged by size:
648 599 402 156 127
Bsu36I CC'TnA_GG
Cuts at: 0 685 1932
Size: 685 1247
BtgI C'CryG_G
Cuts at: 0 459 540 629 887 900 1932
Size: 459 81 89 258 13 1032
Fragments arranged by size:
1032 459 258 89 81 13
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 1104 1932
Size: 1104 828
DrdI GACnn_nn'nnGTC
Cuts at: 0 1288 1932
Size: 1288 644
EaeI y'GGCC_r
Cuts at: 0 83 434 903 941 1932
Size: 83 351 469 38 991
Fragments arranged by size:
991 469 351 83 38
EarI CTCTTCn'nnn_
Cuts at: 0 221 1009 1580 1932
Size: 221 788 571 352
Fragments arranged by size:
788 571 352 221
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 1003 1932
Size: 1003 929
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 187 310 520 868 1588 1780 1831 1863
Size: 187 123 210 348 720 192 51 32
Cuts at: 1863 1932
Size: 69
Fragments arranged by size:
720 348 210 192 187 123 69 51
32
EcoO109I rG'GnC_Cy
Cuts at: 0 16 129 690 777 1146 1442 1932
Size: 16 113 561 87 369 296 490
Fragments arranged by size:
561 490 369 296 113 87 16
FspI TGC'GCA
Cuts at: 0 1180 1932
Size: 1180 752
HaeII r_GCGC'y
Cuts at: 0 27 100 115 1932
Size: 27 73 15 1817
Fragments arranged by size:
1817 73 27 15
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 787 819 1708 1740 1932
Size: 787 32 889 32 192
Fragments arranged by size:
889 787 192 32 32
HincII GTy'rAC
Cuts at: 0 1749 1932
Size: 1749 183
KpnI G_GTAC'C
Cuts at: 0 1854 1932
Size: 1854 78
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 1511 1932
Size: 1511 421
MscI TGG'CCA
Cuts at: 0 436 943 1932
Size: 436 507 989
Fragments arranged by size:
989 507 436
MslI CAynn'nnrTG
Cuts at: 0 211 787 928 1540 1932
Size: 211 576 141 612 392
Fragments arranged by size:
612 576 392 211 141
MspA1I CmG'CkG
Cuts at: 0 402 534 550 1218 1932
Size: 402 132 16 668 714
Fragments arranged by size:
714 668 402 132 16
NaeI GCC'GGC
Cuts at: 0 28 1932
Size: 28 1904
NcoI C'CATG_G
Cuts at: 0 540 1932
Size: 540 1392
NgoMIV G'CCGG_C
Cuts at: 0 26 1932
Size: 26 1906
NspI r_CATG'y
Cuts at: 0 651 765 792 1932
Size: 651 114 27 1140
Fragments arranged by size:
1140 651 114 27
PasI CC'CwG_GG
Cuts at: 0 773 1704 1932
Size: 773 931 228
Fragments arranged by size:
931 773 228
PfoI T'CCnGG_A
Cuts at: 0 1808 1932
Size: 1808 124
PpuMI rG'GwC_Cy
Cuts at: 0 16 129 690 1932
Size: 16 113 561 1242
Fragments arranged by size:
1242 561 113 16
PshAI GACnn'nnGTC
Cuts at: 0 1636 1932
Size: 1636 296
PspOMI G'GGCC_C
Cuts at: 0 776 1146 1932
Size: 776 370 786
Fragments arranged by size:
786 776 370
PsrI GAACnnnnnnTACnnnnnnn_nnnnn'
Cuts at: 0 1453 1485 1932
Size: 1453 32 447
Fragments arranged by size:
1453 447 32
PstI C_TGCA'G
Cuts at: 0 407 1849 1932
Size: 407 1442 83
Fragments arranged by size:
1442 407 83
PvuII CAG'CTG
Cuts at: 0 402 534 550 1932
Size: 402 132 16 1382
Fragments arranged by size:
1382 402 132 16
SalI G'TCGA_C
Cuts at: 0 1747 1932
Size: 1747 185
SanDI GG'GwC_CC
Cuts at: 0 16 129 1932
Size: 16 113 1803
Fragments arranged by size:
1803 113 16
SbfI CC_TGCA'GG
Cuts at: 0 1849 1932
Size: 1849 83
SfcI C'TryA_G
Cuts at: 0 403 1029 1845 1932
Size: 403 626 816 87
Fragments arranged by size:
816 626 403 87
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1145 1932
Size: 1145 787
SmaI CCC'GGG
Cuts at: 0 50 250 278 417 1732 1932
Size: 50 200 28 139 1315 200
Fragments arranged by size:
1315 200 200 139 50 28
SphI G_CATG'C
Cuts at: 0 765 1932
Size: 765 1167
SrfI GCCC'GGGC
Cuts at: 0 50 417 1932
Size: 50 367 1515
Fragments arranged by size:
1515 367 50
StuI AGG'CCT
Cuts at: 0 79 1932
Size: 79 1853
StyI C'CwwG_G
Cuts at: 0 80 540 1337 1932
Size: 80 460 797 595
Fragments arranged by size:
797 595 460 80
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 970 991 1300 1932
Size: 970 21 309 632
Fragments arranged by size:
970 632 309 21
TatI w'GTAC_w
Cuts at: 0 475 1352 1932
Size: 475 877 580
Fragments arranged by size:
877 580 475
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 363 395 493 525 1775 1807 1932
Size: 363 32 98 32 1250 32 125
Fragments arranged by size:
1250 363 125 98 32 32 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 538 789 1625 1932
Size: 538 251 836 307
Fragments arranged by size:
836 538 307 251
XmaI C'CCGG_G
Cuts at: 0 48 248 276 415 1730 1932
Size: 48 200 28 139 1315 202
Fragments arranged by size:
1315 202 200 139 48 28
XmnI GAAnn'nnTTC
Cuts at: 0 1072 1531 1932
Size: 1072 459 401
Fragments arranged by size:
1072 459 401
ZraI GAC'GTC
Cuts at: 0 1638 1875 1932
Size: 1638 237 57
Fragments arranged by size:
1638 237 57
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AleI AlfI
AlwNI ApaI ApaLI ApoI AvaI BamHI BanI BanII
BbsI BbvCI BglI BglII Bme1580I BmgBI BmrI BpmI
Bpu10I BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrFI BssHII
BssSI BstAPI BstEII BstXI BstYI Bsu36I BtgI BtgZI
DrdI EaeI EarI EciI Eco57MI EcoO109I FspI HaeII
Hin4I HincII KpnI MmeI MscI MslI MspA1I NaeI
NcoI NgoMIV NspI PasI PfoI PpuMI PshAI PspOMI
PsrI PstI PvuII SalI SanDI SbfI SfcI SfiI
SmaI SphI SrfI StuI StyI TaqII TatI TstI
XcmI XmaI XmnI ZraI
Enzymes that do not cut:
AfeI AflII AflIII AgeI AhdI AloI AscI AseI
AsiSI AvrII BaeI BbeI BcgI BciVI BclI BfrBI
BlpI BmtI BplI BpuEI BsaI BsaAI BsaBI BsiEI
BsiWI BsmI BsrDI BsrGI BstBI BstZ17I BtsI ClaI
CspCI DraI DraIII EagI EcoICRI EcoNI EcoRI EcoRV
FalI FseI FspAI HindIII HpaI KasI MfeI MluI
NarI NdeI NheI NotI NruI NsiI PacI PciI
PflMI PmeI PmlI PpiI PsiI PspXI PvuI RsrII
SacI SacII SapI ScaI SexAI SfoI SgfI SgrAI
SmlI SnaBI SpeI SspI SwaI Tth111I XbaI XhoI