(Circular) (Six-base) MAPSORT of: pFN21ASDA0152.seq Check: 9385 from: 1 to: 5605 pFN21ASDA0152 5605 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3415 278 Size: 53 83 186 936 1879 2468 Fragments arranged by size: 2468 1879 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2412 2948 1937 Size: 475 536 4594 Fragments arranged by size: 4594 536 475 Acc65I G'GTAC_C Cuts at: 2926 2926 Size: 5605 AclI AA'CG_TT Cuts at: 1563 3732 4105 1563 Size: 2169 373 3063 Fragments arranged by size: 3063 2169 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2669 3668 5052 5320 1632 Size: 1037 999 1384 268 1917 Fragments arranged by size: 1917 1384 1037 999 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5408 AflIII A'CryG_T Cuts at: 1203 4504 5481 1203 Size: 3301 977 1327 Fragments arranged by size: 3301 1327 977 AgeI A'CCGG_T Cuts at: 4436 4436 Size: 5605 AhdI GACnn_n'nnGTC Cuts at: 4334 4334 Size: 5605 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5605 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2294 1184 Size: 1110 4495 AlwNI CAG_nnn'CTG Cuts at: 2314 3348 4920 2314 Size: 1034 1572 2999 Fragments arranged by size: 2999 1572 1034 ApaLI G'TGCA_C Cuts at: 2207 2640 3662 4818 2207 Size: 433 1022 1156 2994 Fragments arranged by size: 2994 1156 1022 433 ApoI r'AATT_y Cuts at: 1493 2399 2914 3140 3292 5401 1493 Size: 906 515 226 152 2109 1697 Fragments arranged by size: 2109 1697 906 515 226 152 AseI AT'TA_AT Cuts at: 160 1032 4159 160 Size: 872 3127 1606 Fragments arranged by size: 3127 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5605 AvaI C'yCGr_G Cuts at: 1870 1943 2108 2156 2930 1870 Size: 73 165 48 774 4545 Fragments arranged by size: 4545 774 165 73 48 BamHI G'GATC_C Cuts at: 2214 2935 3302 2214 Size: 721 367 4517 Fragments arranged by size: 4517 721 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2926 4381 618 Size: 325 217 627 1139 1455 1842 Fragments arranged by size: 1842 1455 1139 627 325 217 BanII G_rGCy'C Cuts at: 1391 2115 2924 1391 Size: 724 809 4072 Fragments arranged by size: 4072 809 724 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2857 928 Size: 971 958 3676 Fragments arranged by size: 3676 971 958 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3796 3830 1903 Size: 34 1859 34 3678 Fragments arranged by size: 3678 1859 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3497 4707 3497 Size: 1210 4395 BclI T'GATC_A Cuts at: 1548 1548 Size: 5605 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2129 2138 2250 Size: 107 122 71 631 605 457 9 112 Cuts at: 2250 4216 136 Size: 1966 1525 Fragments arranged by size: 1966 1525 631 605 457 122 112 107 71 9 BglII A'GATC_T Cuts at: 5600 5600 Size: 5605 BlpI GC'TnA_GC Cuts at: 3022 3022 Size: 5605 Bme1580I G_kGCm'C Cuts at: 1790 2211 2364 2644 3666 4822 1790 Size: 421 153 280 1022 1156 2573 Fragments arranged by size: 2573 1156 1022 421 280 153 BmgBI CAC'GTC Cuts at: 1318 2206 2464 1318 Size: 888 258 4459 Fragments arranged by size: 4459 888 258 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3336 4294 442 Size: 416 53 83 399 24 1919 958 1753 Fragments arranged by size: 1919 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5605 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 4265 1239 Size: 3026 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3730 4595 4893 3730 Size: 865 298 4442 Fragments arranged by size: 4442 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2336 4268 882 Size: 617 837 1932 2219 Fragments arranged by size: 2219 1932 837 617 BsaAI yAC'GTr Cuts at: 493 1204 2386 2902 3396 493 Size: 711 1182 516 494 2702 Fragments arranged by size: 2702 1182 711 516 494 BsaBI GATnn'nnATC Cuts at: 5599 5599 Size: 5605 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3412 3794 4429 275 Size: 53 83 186 936 1879 382 635 1451 Fragments arranged by size: 1879 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4038 4436 4710 4857 5268 4038 Size: 398 274 147 411 4375 Fragments arranged by size: 4375 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5380 Fragments arranged by size: 5380 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2354 2387 1186 Size: 1168 33 4404 Fragments arranged by size: 4404 1168 33 BseYI C'CCAG_C Cuts at: 2026 2427 2657 4808 5304 2026 Size: 401 230 2151 496 2327 Fragments arranged by size: 2327 2151 496 401 230 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2804 1163 Size: 573 1068 3964 Fragments arranged by size: 3964 1068 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3055 3816 4844 1540 Size: 275 186 1054 761 1028 2301 Fragments arranged by size: 2301 1054 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2211 2644 3666 3751 4822 2211 Size: 433 1022 85 1071 2994 Fragments arranged by size: 2994 1071 1022 433 85 BsmI GAATG_Cn' Cuts at: 3116 3209 3116 Size: 93 5512 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2115 2211 2364 2644 2924 3666 3751 Size: 399 325 96 153 280 280 742 85 Cuts at: 3751 4822 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 325 280 280 153 96 85 BspEI T'CCGG_A Cuts at: 5268 5268 Size: 5605 BspHI T'CATG_A Cuts at: 3494 5224 3494 Size: 1730 3875 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2960 844 Size: 2116 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2329 3492 1394 Size: 429 506 1163 3507 Fragments arranged by size: 3507 1163 506 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4100 4274 65 Size: 1183 2852 174 1396 Fragments arranged by size: 2852 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2092 2123 2132 2892 4249 4436 1700 Size: 110 282 31 9 760 1357 187 2869 Fragments arranged by size: 2869 1357 760 282 187 110 31 9 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5605 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5605 BssSI C'ACGA_G Cuts at: 3665 4677 3665 Size: 1012 4593 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5605 BstBI TT'CG_AA Cuts at: 2007 4409 2007 Size: 2402 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5605 BstXI CCAn_nnnn'nTGG Cuts at: 2851 2851 Size: 5605 BstYI r'GATC_y Cuts at: 1972 2214 2310 2935 3302 3687 3704 5156 5242 Size: 242 96 625 367 385 17 1452 86 Cuts at: 5242 5254 5600 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 625 385 367 346 242 96 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2301 2301 Size: 5605 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5053 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4866 Fragments arranged by size: 4866 683 56 BtsI GCAGTG_nn' Cuts at: 3121 3915 3935 3121 Size: 794 20 4791 Fragments arranged by size: 4791 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5605 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2167 2202 529 Size: 35 1603 35 3932 Fragments arranged by size: 3932 1603 35 35 DraI TTT'AAA Cuts at: 2909 2909 Size: 5605 DraIII CAC_nnn'GTG Cuts at: 2467 2467 Size: 5605 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4612 809 Size: 507 3296 1802 Fragments arranged by size: 3296 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2121 2130 2458 Size: 54 1425 50 275 18 291 9 328 Cuts at: 2458 3052 3941 5570 8 Size: 594 889 1629 43 Fragments arranged by size: 1629 1425 889 594 328 291 275 54 50 43 18 9 EagI C'GGCC_G Cuts at: 1537 1812 3052 1537 Size: 275 1240 4090 Fragments arranged by size: 4090 1240 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2709 2885 3535 1353 Size: 1356 176 650 3423 Fragments arranged by size: 3423 1356 650 176 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4192 4566 4712 387 Size: 1064 138 2603 374 146 1280 Fragments arranged by size: 2603 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2669 3668 4265 5052 5320 1239 Size: 393 1037 999 597 787 268 1524 Fragments arranged by size: 1524 1037 999 787 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2238 2238 Size: 5605 EcoO109I rG'GnC_Cy Cuts at: 2105 2230 2792 2105 Size: 125 562 4918 Fragments arranged by size: 4918 562 125 EcoRI G'AATT_C Cuts at: 2914 3292 2914 Size: 378 5227 EcoRV GAT'ATC Cuts at: 2485 2485 Size: 5605 FspI TGC'GCA Cuts at: 4111 4111 Size: 5605 HaeII r_GCGC'y Cuts at: 1678 2063 4752 1678 Size: 385 2689 2531 Fragments arranged by size: 2689 2531 385 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2097 2129 2302 2334 2732 Size: 32 135 32 525 32 173 32 398 Cuts at: 2732 2764 4246 4278 4320 4352 1373 Size: 32 1482 32 42 32 2626 Fragments arranged by size: 2626 1482 525 398 173 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2179 2949 3195 669 Size: 988 281 241 770 246 3079 Fragments arranged by size: 3079 988 770 281 246 241 HindIII A'AGCT_T Cuts at: 748 2681 748 Size: 1933 3672 HpaI GTT'AAC Cuts at: 3195 3195 Size: 5605 KpnI G_GTAC'C Cuts at: 2930 2930 Size: 5605 MfeI C'AATT_G Cuts at: 3204 3204 Size: 5605 MluI A'CGCG_T Cuts at: 5481 5481 Size: 5605 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2319 2897 4719 4903 1305 Size: 1014 578 1822 184 2007 Fragments arranged by size: 2007 1822 1014 578 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2460 10 Size: 54 1425 343 628 3155 Fragments arranged by size: 3155 1425 628 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2849 3563 3922 4081 518 Size: 648 1683 714 359 159 2042 Fragments arranged by size: 2042 1683 714 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2054 3021 3698 4846 5091 1606 Size: 448 967 677 1148 245 2120 Fragments arranged by size: 2120 1148 967 677 448 245 NaeI GCC'GGC Cuts at: 2094 2125 2134 2094 Size: 31 9 5565 Fragments arranged by size: 5565 31 9 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5053 NdeI CA'TA_TG Cuts at: 387 387 Size: 5605 NgoMIV G'CCGG_C Cuts at: 2092 2123 2132 2092 Size: 31 9 5565 Fragments arranged by size: 5565 31 9 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5605 NotI GC'GGCC_GC Cuts at: 3052 3052 Size: 5605 NspI r_CATG'y Cuts at: 1132 1229 2201 2963 4508 1132 Size: 97 972 762 1545 2229 Fragments arranged by size: 2229 1545 972 762 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5605 PciI A'CATG_T Cuts at: 4504 4504 Size: 5605 PflMI CCAn_nnn'nTGG Cuts at: 1108 2467 1108 Size: 1359 4246 PfoI T'CCnGG_A Cuts at: 2695 2695 Size: 5605 PmeI GTTT'AAAC Cuts at: 2909 2909 Size: 5605 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3674 3706 5187 5219 3674 Size: 32 1481 32 4060 Fragments arranged by size: 4060 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2105 2230 2105 Size: 125 5480 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5605 PsiI TTA'TAA Cuts at: 3175 3175 Size: 5605 PstI C_TGCA'G Cuts at: 830 2957 830 Size: 2127 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5605 SalI G'TCGA_C Cuts at: 1936 2947 1936 Size: 1011 4594 SanDI GG'GwC_CC Cuts at: 2105 2105 Size: 5605 SbfI CC_TGCA'GG Cuts at: 2957 2957 Size: 5605 ScaI AGT'ACT Cuts at: 3853 5565 3853 Size: 1712 3893 SfcI C'TryA_G Cuts at: 826 1045 2953 4088 4769 4960 826 Size: 219 1908 1135 681 191 1471 Fragments arranged by size: 1908 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2129 2250 2129 Size: 121 5484 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5605 SgrAI Cr'CCGG_yG Cuts at: 1700 2092 1700 Size: 392 5213 SmaI CCC'GGG Cuts at: 1872 2110 2932 1872 Size: 238 822 4545 Fragments arranged by size: 4545 822 238 SmlI C'TyrA_G Cuts at: 820 1017 1943 3709 4610 4872 820 Size: 197 926 1766 901 262 1553 Fragments arranged by size: 1766 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2902 3396 493 Size: 2409 494 2702 Fragments arranged by size: 2702 2409 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5605 SphI G_CATG'C Cuts at: 1132 2963 1132 Size: 1831 3774 SspI AAT'ATT Cuts at: 5 52 3529 5 Size: 47 3477 2081 Fragments arranged by size: 3477 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5605 StyI C'CwwG_G Cuts at: 513 1065 2631 3044 513 Size: 552 1566 413 3074 Fragments arranged by size: 3074 1566 552 413 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2818 3632 3649 3802 3987 4432 325 Size: 1255 1238 814 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1238 814 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3851 5563 96 Size: 274 80 33 51 1443 1874 1712 138 Fragments arranged by size: 1874 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2040 2072 2542 2574 5565 5597 2040 Size: 32 470 32 2991 32 2048 Fragments arranged by size: 2991 2048 470 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5605 XbaI T'CTAG_A Cuts at: 2941 2941 Size: 5605 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5605 XmaI C'CCGG_G Cuts at: 1870 2108 2930 1870 Size: 238 822 4545 Fragments arranged by size: 4545 822 238 XmnI GAAnn'nnTTC Cuts at: 3734 3734 Size: 5605 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3413 276 Size: 53 83 186 936 1879 2468 Fragments arranged by size: 2468 1879 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SanDI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I EcoICRI FalI FseI FspAI KasI NarI NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SapI SexAI SfoI SrfI SwaI XcmI