Restriction map for
pFN21ASDA0150
(Circular) (Six-base) MAPSORT of: pFN21ASDA0150.seq Check: 8160 from: 1 to: 7573
pFN21ASDA0150 7573 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3427 4053 4151 3427
Size: 626 98 6849
Fragments arranged by size:
6849 626 98
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 5383 278
Size: 53 83 186 936 3847 2468
Fragments arranged by size:
3847 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4916 1937
Size: 2979 4594
Acc65I G'GTAC_C
Cuts at: 2117 4894 2117
Size: 2777 4796
AclI AA'CG_TT
Cuts at: 1563 5700 6073 1563
Size: 4137 373 3063
Fragments arranged by size:
4137 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 3512 4241 4506 5636 7020 7288 1632
Size: 1880 729 265 1130 1384 268 1917
Fragments arranged by size:
1917 1880 1384 1130 729 268 265
AfeI AGC'GCT
Cuts at: 4082 4082
Size: 7573
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 7376
AflIII A'CryG_T
Cuts at: 1203 4260 4266 6472 7449 1203
Size: 3057 6 2206 977 1327
Fragments arranged by size:
3057 2206 1327 977 6
AgeI A'CCGG_T
Cuts at: 6404 6404
Size: 7573
AhdI GACnn_n'nnGTC
Cuts at: 6302 6302
Size: 7573
AleI CACnn'nnGTG
Cuts at: 1166 2741 1166
Size: 1575 5998
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4741 1184
Size: 3557 4016
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2749 2781 2749
Size: 32 7541
AlwNI CAG_nnn'CTG
Cuts at: 2071 2132 3137 4245 4740 5316 6888 2071
Size: 61 1005 1108 495 576 1572 2756
Fragments arranged by size:
2756 1572 1108 1005 576 495 61
ApaI G_GGCC'C
Cuts at: 2317 2446 2867 3004 3565 3718 4467 4512 4810
Size: 129 421 137 561 153 749 45 298
Cuts at: 4810 4819 2317
Size: 9 5071
Fragments arranged by size:
5071 749 561 421 298 153 137 129
45 9
ApaLI G'TGCA_C
Cuts at: 4120 5630 6786 4120
Size: 1510 1156 4907
Fragments arranged by size:
4907 1510 1156
ApoI r'AATT_y
Cuts at: 1493 2465 4882 5108 5260 7369 1493
Size: 972 2417 226 152 2109 1697
Fragments arranged by size:
2417 2109 1697 972 226 152
AseI AT'TA_AT
Cuts at: 160 1032 6127 160
Size: 872 5095 1606
Fragments arranged by size:
5095 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7573
AvaI C'yCGr_G
Cuts at: 1870 1943 2263 2316 2499 2523 2542 2658 2898
Size: 73 320 53 183 24 19 116 240
Cuts at: 2898 2985 4103 4898 1870
Size: 87 1118 795 4545
Fragments arranged by size:
4545 1118 795 320 240 183 116 87
73 53 24 19
AvrII C'CTAG_G
Cuts at: 4811 4811
Size: 7573
BamHI G'GATC_C
Cuts at: 3934 4903 5270 3934
Size: 969 367 6237
Fragments arranged by size:
6237 969 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2117 3410 4139 4391 4690
Size: 325 217 627 330 1293 729 252 299
Cuts at: 4690 4894 6349 618
Size: 204 1455 1842
Fragments arranged by size:
1842 1455 1293 729 627 330 325 299
252 217 204
BanII G_rGCy'C
Cuts at: 1391 2317 2446 2456 2520 2667 2867 3004 3099
Size: 926 129 10 64 147 200 137 95
Cuts at: 3099 3565 3718 3800 4467 4512 4784 4792 4810
Size: 466 153 82 667 45 272 8 18
Cuts at: 4810 4819 4892 1391
Size: 9 73 4072
Fragments arranged by size:
4072 926 667 466 272 200 153 147
137 129 95 82 73 64 45 18
10 9 8
BbeI G_GCGC'C
Cuts at: 4395 4395
Size: 7573
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3503 3662 928
Size: 971 1604 159 4839
Fragments arranged by size:
4839 1604 971 159
BbvCI CC'TCA_GC
Cuts at: 3443 3443
Size: 7573
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 5764 5798 1903
Size: 34 3827 34 3678
Fragments arranged by size:
3827 3678 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 5465 6675 5465
Size: 1210 6363
BclI T'GATC_A
Cuts at: 1548 3627 1548
Size: 2079 5494
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2202 2230 2441
Size: 107 122 71 631 605 530 28 211
Cuts at: 2441 2545 2702 2876 2903 3709 3971 4814 6184
Size: 104 157 174 27 806 262 843 1370
Cuts at: 6184 136
Size: 1525
Fragments arranged by size:
1525 1370 843 806 631 605 530 262
211 174 157 122 107 104 71 28
27
BglII A'GATC_T
Cuts at: 7568 7568
Size: 7573
BlpI GC'TnA_GC
Cuts at: 4990 4990
Size: 7573
Bme1580I G_kGCm'C
Cuts at: 1790 2179 2317 2446 2867 3004 3565 3718 4124
Size: 389 138 129 421 137 561 153 406
Cuts at: 4124 4142 4358 4467 4512 4810 4819 5634 6790
Size: 18 216 109 45 298 9 815 1156
Cuts at: 6790 1790
Size: 2573
Fragments arranged by size:
2573 1156 815 561 421 406 389 298
216 153 138 137 129 109 45 18
9
BmgBI CAC'GTC
Cuts at: 1318 3120 4449 4862 1318
Size: 1802 1329 413 4029
Fragments arranged by size:
4029 1802 1329 413
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3242 3885 4390
Size: 416 53 83 399 24 1825 643 505
Cuts at: 4390 5304 6262 442
Size: 914 958 1753
Fragments arranged by size:
1825 1753 958 914 643 505 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 7573
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3860 3860
Size: 7573
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2503 3151 3414 4685 6233 1239
Size: 1264 648 263 1271 1548 2579
Fragments arranged by size:
2579 1548 1271 1264 648 263
Bpu10I CC'TnA_GC
Cuts at: 3443 4714 3443
Size: 1271 6302
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2375 5698 6563 6861 2375
Size: 3323 865 298 3087
Fragments arranged by size:
3323 3087 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3314 4498 6236 882
Size: 617 1815 1184 1738 2219
Fragments arranged by size:
2219 1815 1738 1184 617
BsaAI yAC'GTr
Cuts at: 493 1204 4261 4267 4870 5364 493
Size: 711 3057 6 603 494 2702
Fragments arranged by size:
3057 2702 711 603 494 6
BsaBI GATnn'nnATC
Cuts at: 7567 7567
Size: 7573
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3659 4392 4550 5380
Size: 53 83 186 936 2126 733 158 830
Cuts at: 5380 5762 6397 275
Size: 382 635 1451
Fragments arranged by size:
2126 1451 936 830 733 635 382 186
158 83 53
BsaWI w'CCGG_w
Cuts at: 2940 3366 6006 6404 6678 6825 7236 2940
Size: 426 2640 398 274 147 411 3277
Fragments arranged by size:
3277 2640 426 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 3218 3248 3854 3884 4683
Size: 30 165 30 1815 30 606 30 799
Cuts at: 4683 4713 1178
Size: 30 4038
Fragments arranged by size:
4038 1815 799 606 165 30 30 30
30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2962 3172 3175 4633 4636 4639 4642 4645
Size: 1776 210 3 1458 3 3 3 3
Cuts at: 4645 4648 1186
Size: 3 4111
Fragments arranged by size:
4111 1776 1458 210 3 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 2122 2445 2761 3143 3237 4466 4530 4553 4791
Size: 323 316 382 94 1229 64 23 238
Cuts at: 4791 6776 7272 2122
Size: 1985 496 2423
Fragments arranged by size:
2423 1985 1229 496 382 323 316 238
94 64 23
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3594 3998 4572 4832 1163
Size: 573 1858 404 574 260 3904
Fragments arranged by size:
3904 1858 574 573 404 260
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2303 5023 5784 6812 1540
Size: 275 186 302 2720 761 1028 2301
Fragments arranged by size:
2720 2301 1028 761 302 275 186
BsiHKAI G_wGCw'C
Cuts at: 2791 4124 4211 4730 5634 5719 6790 2791
Size: 1333 87 519 904 85 1071 3574
Fragments arranged by size:
3574 1333 1071 904 519 87 85
BsmI GAATG_Cn'
Cuts at: 5084 5177 5084
Size: 93 7480
BsmBI CGTCTCn'nnnn_
Cuts at: 3364 3364
Size: 7573
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2179 2317 2446 2456 2520 2667 2791
Size: 399 389 138 129 10 64 147 124
Cuts at: 2791 2867 3004 3099 3565 3718 3800 4124 4142
Size: 76 137 95 466 153 82 324 18
Cuts at: 4142 4211 4358 4467 4512 4730 4784 4792 4810
Size: 69 147 109 45 218 54 8 18
Cuts at: 4810 4819 4892 5634 5719 6790 1391
Size: 9 73 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 466 399 389 324 218
153 147 147 138 137 129 124 109
95 85 82 76 73 69 64 54
45 18 18 10 9 8
BspEI T'CCGG_A
Cuts at: 2940 3366 7236 2940
Size: 426 3870 3277
Fragments arranged by size:
3870 3277 426
BspHI T'CATG_A
Cuts at: 5462 7192 5462
Size: 1730 5843
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2576 3427 4053 4151 4172 4928 844
Size: 1732 851 626 98 21 756 3489
Fragments arranged by size:
3489 1732 851 756 626 98 21
BsrBI CCG'CTC
Cuts at: 1394 1823 2723 3328 5460 1394
Size: 429 900 605 2132 3507
Fragments arranged by size:
3507 2132 900 605 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2107 6068 6242 65
Size: 1183 859 3961 174 1396
Fragments arranged by size:
3961 1396 1183 859 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2875 3215 3661 3927 4201 6217 6404
Size: 110 1065 340 446 266 274 2016 187
Cuts at: 6404 1700
Size: 2869
Fragments arranged by size:
2869 2016 1065 446 340 274 266 187
110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 7573
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 7573
BssSI C'ACGA_G
Cuts at: 2249 4685 5633 6645 2249
Size: 2436 948 1012 3177
Fragments arranged by size:
3177 2436 1012 948
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3601 794
Size: 2807 4766
BstBI TT'CG_AA
Cuts at: 2007 6377 2007
Size: 4370 3203
BstEII G'GTnAC_C
Cuts at: 1184 3523 1184
Size: 2339 5234
BstXI CCAn_nnnn'nTGG
Cuts at: 3742 3742
Size: 7573
BstYI r'GATC_y
Cuts at: 1972 2052 2581 3934 4903 5270 5655 5672 7124
Size: 80 529 1353 969 367 385 17 1452
Cuts at: 7124 7210 7222 7568 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 1353 969 529 385 367 346
86 80 17 12
Bsu36I CC'TnA_GG
Cuts at: 2437 2881 3467 2437
Size: 444 586 6543
Fragments arranged by size:
6543 586 444
BtgI C'CryG_G
Cuts at: 513 1065 2022 2095 2218 2775 4072 4387 4504
Size: 552 957 73 123 557 1297 315 117
Cuts at: 4504 4581 513
Size: 77 3505
Fragments arranged by size:
3505 1297 957 557 552 315 123 117
77 73
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6834
Fragments arranged by size:
6834 683 56
BtsI GCAGTG_nn'
Cuts at: 2578 2647 3981 4569 4597 5089 5883 5903 2578
Size: 69 1334 588 28 492 794 20 4248
Fragments arranged by size:
4248 1334 794 588 492 69 28 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 7573
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3045 3080 529
Size: 35 2481 35 5022
Fragments arranged by size:
5022 2481 35 35
DraI TTT'AAA
Cuts at: 4877 4877
Size: 7573
DraIII CAC_nnn'GTG
Cuts at: 4183 4183
Size: 7573
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 6580 809
Size: 507 5264 1802
Fragments arranged by size:
5264 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2300 2778 2824
Size: 54 1425 50 275 18 470 478 46
Cuts at: 2824 3883 3963 3990 4113 4497 5020 5909 7538
Size: 1059 80 27 123 384 523 889 1629
Cuts at: 7538 8
Size: 43
Fragments arranged by size:
1629 1425 1059 889 523 478 470 384
275 123 80 54 50 46 43 27
18
EagI C'GGCC_G
Cuts at: 1537 1812 2300 5020 1537
Size: 275 488 2720 4090
Fragments arranged by size:
4090 2720 488 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2215 2592 2972 3193 3341 3470 4019 5503
Size: 862 377 380 221 148 129 549 1484
Cuts at: 5503 1353
Size: 3423
Fragments arranged by size:
3423 1484 862 549 380 377 221 148
129
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 2045 3469 3826 6160 6534 6680
Size: 1064 138 456 1424 357 2334 374 146
Cuts at: 6680 387
Size: 1280
Fragments arranged by size:
2334 1424 1280 1064 456 374 357 146
138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2503 3151 3414 3512 4241 4506 4685
Size: 393 871 648 263 98 729 265 179
Cuts at: 4685 5636 6233 7020 7288 1239
Size: 951 597 787 268 1524
Fragments arranged by size:
1524 951 871 787 729 648 597 393
268 265 263 179 98
EcoNI CCTnn'n_nnAGG
Cuts at: 2063 3518 4826 2063
Size: 1455 1308 4810
Fragments arranged by size:
4810 1455 1308
EcoO109I rG'GnC_Cy
Cuts at: 2267 2313 2442 2496 2565 2863 2864 2885 3000
Size: 46 129 54 69 298 1 21 115
Cuts at: 3000 3001 3261 3463 3511 3738 3841 3909 4161
Size: 1 260 202 48 227 103 68 252
Cuts at: 4161 4463 4807 4815 4816 2267
Size: 302 344 8 1 5024
Fragments arranged by size:
5024 344 302 298 260 252 227 202
129 115 103 69 68 54 48 46
21 8 1 1 1
EcoRI G'AATT_C
Cuts at: 4882 5260 4882
Size: 378 7195
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 2603 2725 2603
Size: 122 7451
FspI TGC'GCA
Cuts at: 3920 6079 3920
Size: 2159 5414
HaeII r_GCGC'y
Cuts at: 1678 3681 4084 4395 4537 6720 1678
Size: 2003 403 311 142 2183 2531
Fragments arranged by size:
2531 2183 2003 403 311 142
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3218 3250 3450 3482 4679
Size: 32 135 32 1646 32 200 32 1197
Cuts at: 4679 4711 6214 6246 6288 6320 1373
Size: 32 1503 32 42 32 2626
Fragments arranged by size:
2626 1646 1503 1197 200 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4917 5163 669
Size: 988 281 2979 246 3079
Fragments arranged by size:
3079 2979 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 7573
HpaI GTT'AAC
Cuts at: 5163 5163
Size: 7573
KasI G'GCGC_C
Cuts at: 4391 4391
Size: 7573
KpnI G_GTAC'C
Cuts at: 2121 4898 2121
Size: 2777 4796
MfeI C'AATT_G
Cuts at: 5172 5172
Size: 7573
MluI A'CGCG_T
Cuts at: 7449 7449
Size: 7573
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 3189 4755 6687 6871 1305
Size: 1884 1566 1932 184 2007
Fragments arranged by size:
2007 1932 1884 1566 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2780 2826 3965 3992 10
Size: 54 1425 343 948 46 1139 27 3591
Fragments arranged by size:
3591 1425 1139 948 343 54 46 27
MslI CAynn'nnrTG
Cuts at: 518 1166 2100 2741 5531 5890 6049 518
Size: 648 934 641 2790 359 159 2042
Fragments arranged by size:
2790 2042 934 648 641 359 159
MspA1I CmG'CkG
Cuts at: 1606 2449 2533 2784 3774 3828 3948 4389 4405
Size: 843 84 251 990 54 120 441 16
Cuts at: 4405 4989 5666 6814 7059 1606
Size: 584 677 1148 245 2120
Fragments arranged by size:
2120 1148 990 843 677 584 441 251
245 120 84 54 16
NaeI GCC'GGC
Cuts at: 3217 3663 3929 3217
Size: 446 266 6861
Fragments arranged by size:
6861 446 266
NarI GG'CG_CC
Cuts at: 4392 4392
Size: 7573
NcoI C'CATG_G
Cuts at: 513 1065 2022 2218 513
Size: 552 957 196 5868
Fragments arranged by size:
5868 957 552 196
NdeI CA'TA_TG
Cuts at: 387 387
Size: 7573
NgoMIV G'CCGG_C
Cuts at: 3215 3661 3927 3215
Size: 446 266 6861
Fragments arranged by size:
6861 446 266
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 7573
NotI GC'GGCC_GC
Cuts at: 5020 5020
Size: 7573
NspI r_CATG'y
Cuts at: 1132 1229 2351 3599 4045 4931 6476 1132
Size: 97 1122 1248 446 886 1545 2229
Fragments arranged by size:
2229 1545 1248 1122 886 446 97
PasI CC'CwG_GG
Cuts at: 1348 2749 4318 4460 4604 4797 1348
Size: 1401 1569 142 144 193 4124
Fragments arranged by size:
4124 1569 1401 193 144 142
PciI A'CATG_T
Cuts at: 6472 6472
Size: 7573
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2738 1108
Size: 1630 5943
PfoI T'CCnGG_A
Cuts at: 3391 4701 3391
Size: 1310 6263
PmeI GTTT'AAAC
Cuts at: 4877 4877
Size: 7573
PmlI CAC'GTG
Cuts at: 4261 4261
Size: 7573
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 5642 5674 7155 7187 5642
Size: 32 1481 32 6028
Fragments arranged by size:
6028 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2267 2565 2885 3261 3738 3841 4161 2267
Size: 298 320 376 477 103 320 5679
Fragments arranged by size:
5679 477 376 320 320 298 103
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 7573
PsiI TTA'TAA
Cuts at: 5143 5143
Size: 7573
PspOMI G'GGCC_C
Cuts at: 2313 2442 2863 3000 3561 3714 4463 4508 4806
Size: 129 421 137 561 153 749 45 298
Cuts at: 4806 4815 2313
Size: 9 5071
Fragments arranged by size:
5071 749 561 421 298 153 137 129
45 9
PspXI vC'TCGA_Gb
Cuts at: 2542 2542
Size: 7573
PstI C_TGCA'G
Cuts at: 830 2067 2538 2573 3025 3943 4235 4292 4770
Size: 1237 471 35 452 918 292 57 478
Cuts at: 4770 4925 830
Size: 155 3478
Fragments arranged by size:
3478 1237 918 478 471 452 292 155
57 35
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 7573
PvuII CAG'CTG
Cuts at: 2533 2784 3948 4405 2533
Size: 251 1164 457 5701
Fragments arranged by size:
5701 1164 457 251
SacII CC_GC'GG
Cuts at: 4390 4390
Size: 7573
SalI G'TCGA_C
Cuts at: 1936 4915 1936
Size: 2979 4594
SanDI GG'GwC_CC
Cuts at: 2267 2267
Size: 7573
SapI GCTCTTCn'nnn_
Cuts at: 3341 3470 3341
Size: 129 7444
SbfI CC_TGCA'GG
Cuts at: 3943 4925 3943
Size: 982 6591
ScaI AGT'ACT
Cuts at: 4037 5821 7533 4037
Size: 1784 1712 4077
Fragments arranged by size:
4077 1784 1712
SexAI A'CCwGG_T
Cuts at: 4124 4124
Size: 7573
SfcI C'TryA_G
Cuts at: 826 1045 2063 2534 2569 3021 3939 3949 4231
Size: 219 1018 471 35 452 918 10 282
Cuts at: 4231 4252 4288 4766 4921 6056 6737 6928 826
Size: 21 36 478 155 1135 681 191 1471
Fragments arranged by size:
1471 1135 1018 918 681 478 471 452
282 219 191 155 36 35 21 10
SfiI GGCCn_nnn'nGGCC
Cuts at: 2903 4814 2903
Size: 1911 5662
SfoI GGC'GCC
Cuts at: 4393 4393
Size: 7573
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 7573
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 7573
SmaI CCC'GGG
Cuts at: 1872 2265 2318 2501 2660 2900 2987 4900 1872
Size: 393 53 183 159 240 87 1913 4545
Fragments arranged by size:
4545 1913 393 240 183 159 87 53
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2354 2542 5677 6578 6840 820
Size: 197 926 411 188 3135 901 262 1553
Fragments arranged by size:
3135 1553 926 901 411 262 197 188
SnaBI TAC'GTA
Cuts at: 493 4870 5364 493
Size: 4377 494 2702
Fragments arranged by size:
4377 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 7573
SphI G_CATG'C
Cuts at: 1132 3599 4931 1132
Size: 2467 1332 3774
Fragments arranged by size:
3774 2467 1332
SspI AAT'ATT
Cuts at: 5 52 5497 5
Size: 47 5445 2081
Fragments arranged by size:
5445 2081 47
StuI AGG'CCT
Cuts at: 1517 2906 3041 3164 1517
Size: 1389 135 123 5926
Fragments arranged by size:
5926 1389 135 123
StyI C'CwwG_G
Cuts at: 513 1065 2022 2218 2800 3576 3803 4188 4677
Size: 552 957 196 582 776 227 385 489
Cuts at: 4677 4811 4827 5012 513
Size: 134 16 185 3074
Fragments arranged by size:
3074 957 776 582 552 489 385 227
196 185 134 16
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2124 2161 2950 3628 4310 5600 5617
Size: 1255 544 37 789 678 682 1290 17
Cuts at: 5617 5770 5955 6400 325
Size: 153 185 445 1498
Fragments arranged by size:
1498 1290 1255 789 682 678 544 445
185 153 37 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3150 4035 4044
Size: 274 80 33 51 1443 1173 885 9
Cuts at: 4044 5819 7531 96
Size: 1775 1712 138
Fragments arranged by size:
1775 1712 1443 1173 885 274 138 80
51 33 9
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2557 2589 2665 2697 3373 3405 4169 4201 4683
Size: 32 76 32 676 32 764 32 482
Cuts at: 4683 4715 7533 7565 2557
Size: 32 2818 32 2565
Fragments arranged by size:
2818 2565 764 676 482 76 32 32
32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 3540 1316
Size: 2224 5349
XbaI T'CTAG_A
Cuts at: 4909 4909
Size: 7573
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2606 3511 2606
Size: 905 6668
XhoI C'TCGA_G
Cuts at: 1943 2542 1943
Size: 599 6974
XmaI C'CCGG_G
Cuts at: 1870 2263 2316 2499 2658 2898 2985 4898 1870
Size: 393 53 183 159 240 87 1913 4545
Fragments arranged by size:
4545 1913 393 240 183 159 87 53
XmnI GAAnn'nnTTC
Cuts at: 5702 5702
Size: 7573
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 5381 276
Size: 53 83 186 936 3847 2468
Fragments arranged by size:
3847 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI
Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI
BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI
EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI
FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI
MfeI MluI MmeI MscI MslI MspA1I NaeI NarI
NcoI NdeI NgoMIV NheI NotI NspI PasI PciI
PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI
PspOMI PspXI PstI PvuI PvuII SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI
XmaI XmnI ZraI
Enzymes that do not cut:
AscI BaeI BfrBI BsiWI BstZ17I EcoICRI EcoRV FseI
FspAI NruI NsiI PacI PsrI RsrII SacI SrfI
SwaI