(Circular) (Six-base) MAPSORT of: pFN21ASDA0149.seq Check: 960 from: 1 to: 7219 pFN21ASDA0149 7219 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2744 3695 2744 Size: 951 6268 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5029 278 Size: 53 83 186 936 3493 2468 Fragments arranged by size: 3493 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4562 1937 Size: 2625 4594 Acc65I G'GTAC_C Cuts at: 3056 3806 4540 3056 Size: 750 734 5735 Fragments arranged by size: 5735 750 734 AclI AA'CG_TT Cuts at: 1563 5346 5719 1563 Size: 3783 373 3063 Fragments arranged by size: 3783 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4467 5282 6666 6934 1632 Size: 2835 815 1384 268 1917 Fragments arranged by size: 2835 1917 1384 815 268 AfeI AGC'GCT Cuts at: 3005 3005 Size: 7219 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7022 AflIII A'CryG_T Cuts at: 1203 6118 7095 1203 Size: 4915 977 1327 Fragments arranged by size: 4915 1327 977 AgeI A'CCGG_T Cuts at: 4002 6050 4002 Size: 2048 5171 AhdI GACnn_n'nnGTC Cuts at: 5948 5948 Size: 7219 AleI CACnn'nnGTG Cuts at: 1166 2109 2814 1166 Size: 943 705 5571 Fragments arranged by size: 5571 943 705 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3323 1184 Size: 2139 5080 AlwNI CAG_nnn'CTG Cuts at: 2990 3269 3995 4034 4228 4406 4962 6534 2990 Size: 279 726 39 194 178 556 1572 3675 Fragments arranged by size: 3675 1572 726 556 279 194 178 39 ApaI G_GGCC'C Cuts at: 2275 2312 3030 3159 3364 4082 2275 Size: 37 718 129 205 718 5412 Fragments arranged by size: 5412 718 718 205 129 37 ApaLI G'TGCA_C Cuts at: 2731 5276 6432 2731 Size: 2545 1156 3518 Fragments arranged by size: 3518 2545 1156 ApoI r'AATT_y Cuts at: 1493 4528 4754 4906 7015 1493 Size: 3035 226 152 2109 1697 Fragments arranged by size: 3035 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 5773 160 Size: 872 4741 1606 Fragments arranged by size: 4741 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7219 AvaI C'yCGr_G Cuts at: 1870 1943 2064 2293 3771 3882 3953 3980 4075 Size: 73 121 229 1478 111 71 27 95 Cuts at: 4075 4544 1870 Size: 469 4545 Fragments arranged by size: 4545 1478 469 229 121 111 95 73 71 27 BamHI G'GATC_C Cuts at: 4121 4429 4549 4916 4121 Size: 308 120 367 6424 Fragments arranged by size: 6424 367 308 120 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2552 2583 2921 3056 3806 Size: 325 217 627 765 31 338 135 750 Cuts at: 3806 4370 4540 5995 618 Size: 564 170 1455 1842 Fragments arranged by size: 1842 1455 765 750 627 564 338 325 217 170 135 31 BanII G_rGCy'C Cuts at: 1391 2275 2312 2404 3030 3159 3364 3458 3849 Size: 884 37 92 626 129 205 94 391 Cuts at: 3849 4076 4082 4283 4393 4538 1391 Size: 227 6 201 110 145 4072 Fragments arranged by size: 4072 884 626 391 227 205 201 145 129 110 94 92 37 6 BbeI G_GCGC'C Cuts at: 2556 2587 2556 Size: 31 7188 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3045 3075 4138 928 Size: 971 1146 30 1063 4009 Fragments arranged by size: 4009 1146 1063 971 30 BbvCI CC'TCA_GC Cuts at: 3479 3479 Size: 7219 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4081 4115 5410 5444 1903 Size: 34 2144 34 1295 34 3678 Fragments arranged by size: 3678 2144 1295 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5111 6321 5111 Size: 1210 6009 BclI T'GATC_A Cuts at: 1548 1548 Size: 7219 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2658 2745 2784 Size: 107 122 71 631 605 986 87 39 Cuts at: 2784 2919 3264 3572 3698 3874 5830 136 Size: 135 345 308 126 176 1956 1525 Fragments arranged by size: 1956 1525 986 631 605 345 308 176 135 126 122 107 87 71 39 BglII A'GATC_T Cuts at: 7214 7214 Size: 7219 BlpI GC'TnA_GC Cuts at: 2133 4035 4636 2133 Size: 1902 601 4716 Fragments arranged by size: 4716 1902 601 Bme1580I G_kGCm'C Cuts at: 1790 2275 2312 2735 2954 3030 3159 3364 4082 Size: 485 37 423 219 76 129 205 718 Cuts at: 4082 5280 6436 1790 Size: 1198 1156 2573 Fragments arranged by size: 2573 1198 1156 718 485 423 219 205 129 76 37 BmgBI CAC'GTC Cuts at: 1318 2107 1318 Size: 789 6430 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2314 2890 3160 Size: 416 53 83 399 24 897 576 270 Cuts at: 3160 4950 5908 442 Size: 1790 958 1753 Fragments arranged by size: 1790 1753 958 897 576 416 399 270 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7219 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2650 2860 3577 3748 4420 2650 Size: 210 717 171 672 5449 Fragments arranged by size: 5449 717 672 210 171 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2176 2832 3630 3745 3835 4480 5879 1239 Size: 937 656 798 115 90 645 1399 2579 Fragments arranged by size: 2579 1399 937 798 656 645 115 90 Bpu10I CC'TnA_GC Cuts at: 3479 3838 4209 3479 Size: 359 371 6489 Fragments arranged by size: 6489 371 359 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3362 5344 6209 6507 3362 Size: 1982 865 298 4074 Fragments arranged by size: 4074 1982 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3796 5882 882 Size: 617 2297 2086 2219 Fragments arranged by size: 2297 2219 2086 617 BsaAI yAC'GTr Cuts at: 493 1204 2496 4042 4516 5010 493 Size: 711 1292 1546 474 494 2702 Fragments arranged by size: 2702 1546 1292 711 494 474 BsaBI GATnn'nnATC Cuts at: 7213 7213 Size: 7219 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2205 2553 2584 3729 Size: 53 83 186 936 672 348 31 1145 Cuts at: 3729 5026 5408 6043 275 Size: 1297 382 635 1451 Fragments arranged by size: 1451 1297 1145 936 672 635 382 348 186 83 53 31 BsaWI w'CCGG_w Cuts at: 4002 5652 6050 6324 6471 6882 4002 Size: 1650 398 274 147 411 4339 Fragments arranged by size: 4339 1650 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2267 2297 3393 3423 1178 Size: 30 165 30 864 30 1096 30 4974 Fragments arranged by size: 4974 1096 864 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7219 BseYI C'CCAG_C Cuts at: 2029 2394 3022 3158 3178 3357 3570 4301 4360 Size: 365 628 136 20 179 213 731 59 Cuts at: 4360 6422 6918 2029 Size: 2062 496 2330 Fragments arranged by size: 2330 2062 731 628 496 365 213 179 136 59 20 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2819 1163 Size: 573 1083 5563 Fragments arranged by size: 5563 1083 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2392 2457 2641 2657 4669 5430 Size: 275 186 391 65 184 16 2012 761 Cuts at: 5430 6458 1540 Size: 1028 2301 Fragments arranged by size: 2301 2012 1028 761 391 275 186 184 65 16 BsiHKAI G_wGCw'C Cuts at: 2735 2846 3849 4041 4076 5280 5365 6436 2735 Size: 111 1003 192 35 1204 85 1071 3518 Fragments arranged by size: 3518 1204 1071 1003 192 111 85 35 BsmI GAATG_Cn' Cuts at: 2180 2681 3224 4730 4823 2180 Size: 501 543 1506 93 4576 Fragments arranged by size: 4576 1506 543 501 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2275 2312 2404 2735 2846 2954 3030 Size: 399 485 37 92 331 111 108 76 Cuts at: 3030 3159 3364 3458 3849 4041 4076 4082 4283 Size: 129 205 94 391 192 35 6 201 Cuts at: 4283 4393 4538 5280 5365 6436 1391 Size: 110 145 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 485 399 391 331 205 201 192 145 129 111 110 108 94 92 85 76 37 35 6 BspEI T'CCGG_A Cuts at: 6882 6882 Size: 7219 BspHI T'CATG_A Cuts at: 5108 6838 5108 Size: 1730 5489 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2744 3092 3418 3695 4574 844 Size: 1900 348 326 277 879 3489 Fragments arranged by size: 3489 1900 879 348 326 277 BsrBI CCG'CTC Cuts at: 1394 1823 3859 4140 5106 1394 Size: 429 2036 281 966 3507 Fragments arranged by size: 3507 2036 966 429 281 BsrDI GCAATG_nn' Cuts at: 65 1248 5714 5888 65 Size: 1183 4466 174 1396 Fragments arranged by size: 4466 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2456 2652 3143 3182 3566 4002 5863 Size: 110 646 196 491 39 384 436 1861 Cuts at: 5863 6050 1700 Size: 187 2869 Fragments arranged by size: 2869 1861 646 491 436 384 196 187 110 39 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7219 BssHII G'CGCG_C Cuts at: 1925 2531 2759 1925 Size: 606 228 6385 Fragments arranged by size: 6385 606 228 BssSI C'ACGA_G Cuts at: 5279 6291 5279 Size: 1012 6207 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7219 BstBI TT'CG_AA Cuts at: 2007 6023 2007 Size: 4016 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7219 BstXI CCAn_nnnn'nTGG Cuts at: 2029 2029 Size: 7219 BstYI r'GATC_y Cuts at: 1972 4121 4429 4549 4916 5301 5318 6770 6856 Size: 2149 308 120 367 385 17 1452 86 Cuts at: 6856 6868 7214 1972 Size: 12 346 1977 Fragments arranged by size: 2149 1977 1452 385 367 346 308 120 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3723 3903 3723 Size: 180 7039 BtgI C'CryG_G Cuts at: 513 1065 2022 2364 2434 2523 2547 2611 2752 Size: 552 957 342 70 89 24 64 141 Cuts at: 2752 2791 3190 4117 513 Size: 39 399 927 3615 Fragments arranged by size: 3615 957 927 552 399 342 141 89 70 64 39 24 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6480 Fragments arranged by size: 6480 683 56 BtsI GCAGTG_nn' Cuts at: 2147 2275 2467 2693 2807 2954 2956 3145 3282 Size: 128 192 226 114 147 2 189 137 Cuts at: 3282 4735 5529 5549 2147 Size: 1453 794 20 3817 Fragments arranged by size: 3817 1453 794 226 192 189 147 137 128 114 20 2 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7219 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7184 DraI TTT'AAA Cuts at: 4523 4523 Size: 7219 DraIII CAC_nnn'GTG Cuts at: 2797 2797 Size: 7219 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2077 3527 6226 809 Size: 507 761 1450 2699 1802 Fragments arranged by size: 2699 1802 1450 761 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2115 2347 2638 Size: 54 1425 50 275 18 285 232 291 Cuts at: 2638 2654 2818 2992 3782 3997 4273 4666 5555 Size: 16 164 174 790 215 276 393 889 Cuts at: 5555 7184 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 790 393 291 285 276 275 232 215 174 164 54 50 43 18 16 EagI C'GGCC_G Cuts at: 1537 1812 2638 2654 4666 1537 Size: 275 826 16 2012 4090 Fragments arranged by size: 4090 2012 826 275 16 EarI CTCTTCn'nnn_ Cuts at: 1353 3329 3915 4443 5149 1353 Size: 1976 586 528 706 3423 Fragments arranged by size: 3423 1976 706 586 528 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4139 4196 5806 6180 6326 387 Size: 1064 138 2550 57 1610 374 146 1280 Fragments arranged by size: 2550 1610 1280 1064 374 146 138 57 EcoICRI GAG'CTC Cuts at: 3847 4074 3847 Size: 227 6992 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2176 2832 3630 3745 3835 4467 4480 Size: 393 544 656 798 115 90 632 13 Cuts at: 4480 5282 5879 6666 6934 1239 Size: 802 597 787 268 1524 Fragments arranged by size: 1524 802 798 787 656 632 597 544 393 268 115 90 13 EcoO109I rG'GnC_Cy Cuts at: 2271 2308 2309 2669 2892 3026 3105 3155 3252 Size: 37 1 360 223 134 79 50 97 Cuts at: 3252 3361 3529 3899 4079 4130 4225 4322 2271 Size: 109 168 370 180 51 95 97 5168 Fragments arranged by size: 5168 370 360 223 180 168 134 109 97 97 95 79 51 50 37 1 EcoRI G'AATT_C Cuts at: 4528 4906 4528 Size: 378 6841 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4322 4322 Size: 7219 FspI TGC'GCA Cuts at: 2149 2806 5725 2149 Size: 657 2919 3643 Fragments arranged by size: 3643 2919 657 FspAI rTGC'GCAy Cuts at: 2806 2806 Size: 7219 HaeII r_GCGC'y Cuts at: 1678 2443 2556 2587 2718 3007 6366 1678 Size: 765 113 31 131 289 3359 2531 Fragments arranged by size: 3359 2531 765 289 131 113 31 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3062 3094 3360 3392 3575 Size: 32 135 32 1490 32 266 32 183 Cuts at: 3575 3590 3607 3622 5860 5892 5934 5966 1373 Size: 15 17 15 2238 32 42 32 2626 Fragments arranged by size: 2626 2238 1490 266 183 135 42 32 32 32 32 32 32 17 15 15 HincII GTy'rAC Cuts at: 669 1657 1938 4563 4809 669 Size: 988 281 2625 246 3079 Fragments arranged by size: 3079 2625 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7219 HpaI GTT'AAC Cuts at: 4809 4809 Size: 7219 KasI G'GCGC_C Cuts at: 2552 2583 2552 Size: 31 7188 KpnI G_GTAC'C Cuts at: 3060 3810 4544 3060 Size: 750 734 5735 Fragments arranged by size: 5735 750 734 MfeI C'AATT_G Cuts at: 4818 4818 Size: 7219 MluI A'CGCG_T Cuts at: 7095 7095 Size: 7219 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 6333 6517 1305 Size: 5028 184 2007 Fragments arranged by size: 5028 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2117 2994 3999 10 Size: 54 1425 343 285 877 1005 3230 Fragments arranged by size: 3230 1425 1005 877 343 285 54 MslI CAynn'nnrTG Cuts at: 518 1166 2109 2814 5177 5536 5695 518 Size: 648 943 705 2363 359 159 2042 Fragments arranged by size: 2363 2042 943 705 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2047 2289 2394 2406 2525 2589 2601 2643 Size: 441 242 105 12 119 64 12 42 Cuts at: 2643 2709 2754 2814 2823 2862 3162 4119 4635 Size: 66 45 60 9 39 300 957 516 Cuts at: 4635 5312 6460 6705 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 957 677 516 441 300 245 242 119 105 66 64 60 45 42 39 12 12 9 NaeI GCC'GGC Cuts at: 2654 3145 3568 2654 Size: 491 423 6305 Fragments arranged by size: 6305 491 423 NarI GG'CG_CC Cuts at: 2553 2584 2553 Size: 31 7188 NcoI C'CATG_G Cuts at: 513 1065 2022 3190 513 Size: 552 957 1168 4542 Fragments arranged by size: 4542 1168 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7219 NgoMIV G'CCGG_C Cuts at: 2652 3143 3566 2652 Size: 491 423 6305 Fragments arranged by size: 6305 491 423 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7219 NotI GC'GGCC_GC Cuts at: 4666 4666 Size: 7219 NspI r_CATG'y Cuts at: 1132 1229 4102 4577 6122 1132 Size: 97 2873 475 1545 2229 Fragments arranged by size: 2873 2229 1545 475 97 PasI CC'CwG_GG Cuts at: 1348 3498 1348 Size: 2150 5069 PciI A'CATG_T Cuts at: 6118 6118 Size: 7219 PflMI CCAn_nnn'nTGG Cuts at: 1108 2990 3269 4301 4406 1108 Size: 1882 279 1032 105 3921 Fragments arranged by size: 3921 1882 1032 279 105 PfoI T'CCnGG_A Cuts at: 3413 4065 3413 Size: 652 6567 PmeI GTTT'AAAC Cuts at: 4523 4523 Size: 7219 PmlI CAC'GTG Cuts at: 2496 4042 2496 Size: 1546 5673 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5288 5320 6801 6833 5288 Size: 32 1481 32 5674 Fragments arranged by size: 5674 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2669 2892 3105 3252 3529 3899 2669 Size: 223 213 147 277 370 5989 Fragments arranged by size: 5989 370 277 223 213 147 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7219 PsiI TTA'TAA Cuts at: 4789 4789 Size: 7219 PspOMI G'GGCC_C Cuts at: 2271 2308 3026 3155 3360 4078 2271 Size: 37 718 129 205 718 5412 Fragments arranged by size: 5412 718 718 205 129 37 PstI C_TGCA'G Cuts at: 830 2142 2282 2594 2714 3140 3429 3706 4571 Size: 1312 140 312 120 426 289 277 865 Cuts at: 4571 830 Size: 3478 Fragments arranged by size: 3478 1312 865 426 312 289 277 140 120 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7219 PvuII CAG'CTG Cuts at: 2814 2862 3162 2814 Size: 48 300 6871 Fragments arranged by size: 6871 300 48 RsrII CG'GwC_CG Cuts at: 4351 4351 Size: 7219 SacI G_AGCT'C Cuts at: 3849 4076 3849 Size: 227 6992 SacII CC_GC'GG Cuts at: 2526 2755 4120 2526 Size: 229 1365 5625 Fragments arranged by size: 5625 1365 229 SalI G'TCGA_C Cuts at: 1936 4561 1936 Size: 2625 4594 SanDI GG'GwC_CC Cuts at: 2669 2892 2669 Size: 223 6996 SapI GCTCTTCn'nnn_ Cuts at: 3329 3329 Size: 7219 SbfI CC_TGCA'GG Cuts at: 4571 4571 Size: 7219 ScaI AGT'ACT Cuts at: 2303 5467 7179 2303 Size: 3164 1712 2343 Fragments arranged by size: 3164 2343 1712 SfcI C'TryA_G Cuts at: 826 1045 2138 2278 2590 2710 3136 3425 3702 Size: 219 1093 140 312 120 426 289 277 Cuts at: 3702 4567 5702 6383 6574 826 Size: 865 1135 681 191 1471 Fragments arranged by size: 1471 1135 1093 865 681 426 312 289 277 219 191 140 120 SfoI GGC'GCC Cuts at: 2554 2585 2554 Size: 31 7188 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7219 SgrAI Cr'CCGG_yG Cuts at: 1700 4002 1700 Size: 2302 4917 SmaI CCC'GGG Cuts at: 1872 2295 3884 3982 4546 1872 Size: 423 1589 98 564 4545 Fragments arranged by size: 4545 1589 564 423 98 SmlI C'TyrA_G Cuts at: 820 1017 1943 3377 5323 6224 6486 820 Size: 197 926 1434 1946 901 262 1553 Fragments arranged by size: 1946 1553 1434 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4516 5010 493 Size: 4023 494 2702 Fragments arranged by size: 4023 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7219 SphI G_CATG'C Cuts at: 1132 4577 1132 Size: 3445 3774 SrfI GCCC'GGGC Cuts at: 2295 2295 Size: 7219 SspI AAT'ATT Cuts at: 5 52 5143 5 Size: 47 5091 2081 Fragments arranged by size: 5091 2081 47 StuI AGG'CCT Cuts at: 1517 2975 1517 Size: 1458 5761 StyI C'CwwG_G Cuts at: 513 1065 2022 3190 4188 4658 513 Size: 552 957 1168 998 470 3074 Fragments arranged by size: 3074 1168 998 957 552 470 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3490 3778 5246 5263 5416 5601 6046 Size: 1255 1910 288 1468 17 153 185 445 Cuts at: 6046 325 Size: 1498 Fragments arranged by size: 1910 1498 1468 1255 445 288 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2301 5465 7177 Size: 274 80 33 51 1443 324 3164 1712 Cuts at: 7177 96 Size: 138 Fragments arranged by size: 3164 1712 1443 324 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2267 2299 3393 3425 7179 7211 2267 Size: 32 1094 32 3754 32 2275 Fragments arranged by size: 3754 2275 1094 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3131 1316 Size: 1815 5404 XbaI T'CTAG_A Cuts at: 4555 4555 Size: 7219 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3245 3266 4324 3245 Size: 21 1058 6140 Fragments arranged by size: 6140 1058 21 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7219 XmaI C'CCGG_G Cuts at: 1870 2293 3882 3980 4544 1870 Size: 423 1589 98 564 4545 Fragments arranged by size: 4545 1589 564 423 98 XmnI GAAnn'nnTTC Cuts at: 5348 5348 Size: 7219 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5027 276 Size: 53 83 186 936 3493 2468 Fragments arranged by size: 3493 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI FspAI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I EcoNI EcoRV FseI NruI NsiI PacI PspXI PsrI SexAI SfiI SwaI