Restriction map for
pFN21ASDA0147
(Circular) (Six-base) MAPSORT of: pFN21ASDA0147.seq Check: 5122 from: 1 to: 9619
pFN21ASDA0147 9619 bp
With 182 enzymes: SgfI *
January 16, 2009 15:29 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 3266 3266
Size: 9619
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 7429 278
Size: 53 83 186 936 5893 2468
Fragments arranged by size:
5893 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2841 6962 1937
Size: 904 4121 4594
Fragments arranged by size:
4594 4121 904
Acc65I G'GTAC_C
Cuts at: 5869 6940 5869
Size: 1071 8548
AclI AA'CG_TT
Cuts at: 1563 7746 8119 1563
Size: 6183 373 3063
Fragments arranged by size:
6183 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2341 2837 4089 4627 5834 6861 7682 9066
Size: 709 496 1252 538 1207 1027 821 1384
Cuts at: 9066 9334 1632
Size: 268 1917
Fragments arranged by size:
1917 1384 1252 1207 1027 821 709 538
496 268
AfeI AGC'GCT
Cuts at: 5851 5851
Size: 9619
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 9422
AflIII A'CryG_T
Cuts at: 1203 2286 2700 8518 9495 1203
Size: 1083 414 5818 977 1327
Fragments arranged by size:
5818 1327 1083 977 414
AgeI A'CCGG_T
Cuts at: 8450 8450
Size: 9619
AhdI GACnn_n'nnGTC
Cuts at: 3529 8348 3529
Size: 4819 4800
AleI CACnn'nnGTG
Cuts at: 1166 4439 6671 1166
Size: 3273 2232 4114
Fragments arranged by size:
4114 3273 2232
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 4457 5192 1184
Size: 3273 735 5611
Fragments arranged by size:
5611 3273 735
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2357 2389 5313 5345 2357
Size: 32 2924 32 6631
Fragments arranged by size:
6631 2924 32 32
AlwNI CAG_nnn'CTG
Cuts at: 2449 2782 3139 3373 3527 3617 5515 5761 5812
Size: 333 357 234 154 90 1898 246 51
Cuts at: 5812 6232 6251 7362 8934 2449
Size: 420 19 1111 1572 3134
Fragments arranged by size:
3134 1898 1572 1111 420 357 333 246
234 154 90 51 19
ApaI G_GGCC'C
Cuts at: 3638 3938 3994 4590 4880 5060 5729 5775 6140
Size: 300 56 596 290 180 669 46 365
Cuts at: 6140 6499 6618 6839 6902 3638
Size: 359 119 221 63 6355
Fragments arranged by size:
6355 669 596 365 359 300 290 221
180 119 63 56 46
ApaLI G'TGCA_C
Cuts at: 7676 8832 7676
Size: 1156 8463
ApoI r'AATT_y
Cuts at: 1493 6928 7154 7306 9415 1493
Size: 5435 226 152 2109 1697
Fragments arranged by size:
5435 2109 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4776 8173 160
Size: 872 3744 3397 1606
Fragments arranged by size:
3744 3397 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9619
AvaI C'yCGr_G
Cuts at: 1870 1943 2249 2453 2669 2993 3008 3293 3629
Size: 73 306 204 216 324 15 285 336
Cuts at: 3629 3755 3887 3947 3987 4305 4332 4511 4530
Size: 126 132 60 40 318 27 179 19
Cuts at: 4530 4583 4958 5308 5774 6334 6464 6706 6944
Size: 53 375 350 466 560 130 242 238
Cuts at: 6944 1870
Size: 4545
Fragments arranged by size:
4545 560 466 375 350 336 324 318
306 285 242 238 216 204 179 132
130 126 73 60 53 40 27 19
15
BamHI G'GATC_C
Cuts at: 6949 7316 6949
Size: 367 9252
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2682 4391 4963 5302 5338
Size: 325 217 627 895 1709 572 339 36
Cuts at: 5338 5375 5869 6386 6417 6460 6885 6940 8395
Size: 37 494 517 31 43 425 55 1455
Cuts at: 8395 618
Size: 1842
Fragments arranged by size:
1842 1709 1455 895 627 572 517 494
425 339 325 217 55 43 37 36
31
BanII G_rGCy'C
Cuts at: 1391 2496 2608 2670 2739 3465 3630 3638 3723
Size: 1105 112 62 69 726 165 8 85
Cuts at: 3723 3938 3985 3994 4265 4590 4880 5060 5444
Size: 215 47 9 271 325 290 180 384
Cuts at: 5444 5720 5729 5775 6140 6341 6359 6492 6499
Size: 276 9 46 365 201 18 133 7
Cuts at: 6499 6618 6839 6902 6938 1391
Size: 119 221 63 36 4072
Fragments arranged by size:
4072 1105 726 384 365 325 290 276
271 221 215 201 180 165 133 119
112 85 69 63 62 47 46 36
18 9 9 8 7
BbeI G_GCGC'C
Cuts at: 2686 4395 6390 6421 6889 2686
Size: 1709 1995 31 468 5416
Fragments arranged by size:
5416 1995 1709 468 31
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 3594 3702 6684 928
Size: 971 1695 108 2982 3863
Fragments arranged by size:
3863 2982 1695 971 108
BbvCI CC'TCA_GC
Cuts at: 3402 3645 6581 6732 3402
Size: 243 2936 151 6289
Fragments arranged by size:
6289 2936 243 151
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3082 3116 4706 4740 6497 6531 7810
Size: 34 1145 34 1590 34 1757 34 1279
Cuts at: 7810 7844 1903
Size: 34 3678
Fragments arranged by size:
3678 1757 1590 1279 1145 34 34 34
34 34
BciVI GTATCCnnnnn_n'
Cuts at: 7511 8721 7511
Size: 1210 8409
BclI T'GATC_A
Cuts at: 1548 1548
Size: 9619
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 3899 4415 4507
Size: 107 122 71 631 605 2227 516 92
Cuts at: 4507 4616 5391 5520 5724 5987 5996 6503 8230
Size: 109 775 129 204 263 9 507 1727
Cuts at: 8230 136
Size: 1525
Fragments arranged by size:
2227 1727 1525 775 631 605 516 507
263 204 129 122 109 107 92 71
9
BglII A'GATC_T
Cuts at: 2112 3867 9614 2112
Size: 1755 5747 2117
Fragments arranged by size:
5747 2117 1755
BlpI GC'TnA_GC
Cuts at: 2733 3624 5714 7036 2733
Size: 891 2090 1322 5316
Fragments arranged by size:
5316 2090 1322 891
Bme1580I G_kGCm'C
Cuts at: 1790 3638 3938 3994 4590 4745 4880 5060 5254
Size: 1848 300 56 596 155 135 180 194
Cuts at: 5254 5729 5775 5847 6006 6126 6140 6475 6499
Size: 475 46 72 159 120 14 335 24
Cuts at: 6499 6608 6618 6839 6902 6909 7680 8836 1790
Size: 109 10 221 63 7 771 1156 2573
Fragments arranged by size:
2573 1848 1156 771 596 475 335 300
221 194 180 159 155 135 120 109
72 63 56 46 24 14 10 7
BmgBI CAC'GTC
Cuts at: 1318 2287 2461 2701 3115 4789 5218 1318
Size: 969 174 240 414 1674 429 5719
Fragments arranged by size:
5719 1674 969 429 414 240 174
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 4236 5018 5776
Size: 416 53 83 399 24 2819 782 758
Cuts at: 5776 7350 8308 442
Size: 1574 958 1753
Fragments arranged by size:
2819 1753 1574 958 782 758 416 399
83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 9619
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2911 5710 2911
Size: 2799 6820
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2156 2359 2363 2510 2738 3352 3395 4359
Size: 917 203 4 147 228 614 43 964
Cuts at: 4359 4385 5291 5642 5705 5898 5907 6131 6521
Size: 26 906 351 63 193 9 224 390
Cuts at: 6521 6641 8279 1239
Size: 120 1638 2579
Fragments arranged by size:
2579 1638 964 917 906 614 390 351
228 224 203 193 147 120 63 43
26 9 4
Bpu10I CC'TnA_GC
Cuts at: 2219 3402 3645 6144 6581 6732 2219
Size: 1183 243 2499 437 151 5106
Fragments arranged by size:
5106 2499 1183 437 243 151
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2012 2495 3067 3152 3449 6331 7744 8609 8907
Size: 483 572 85 297 2882 1413 865 298
Cuts at: 8907 2012
Size: 2724
Fragments arranged by size:
2882 2724 1413 865 572 483 298 297
85
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 3320 8282 882
Size: 617 1821 4962 2219
Fragments arranged by size:
4962 2219 1821 617
BsaAI yAC'GTr
Cuts at: 493 1204 2062 3106 4606 6916 7410 493
Size: 711 858 1044 1500 2310 494 2702
Fragments arranged by size:
2702 2310 1500 1044 858 711 494
BsaBI GATnn'nnATC
Cuts at: 9613 9613
Size: 9619
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2154 2683 3699 4392
Size: 53 83 186 936 621 529 1016 693
Cuts at: 4392 6362 6387 6418 6712 6886 7426 7808 8443
Size: 1970 25 31 294 174 540 382 635
Cuts at: 8443 275
Size: 1451
Fragments arranged by size:
1970 1451 1016 936 693 635 621 540
529 382 294 186 174 83 53 31
25
BsaWI w'CCGG_w
Cuts at: 2637 2982 3210 5673 5950 6448 6721 8052 8450
Size: 345 228 2463 277 498 273 1331 398
Cuts at: 8450 8724 8871 9282 2637
Size: 274 147 411 2974
Fragments arranged by size:
2974 2463 1331 498 411 398 345 277
274 273 228 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 2357 2387 4332 4362 4773
Size: 30 165 30 954 30 1945 30 411
Cuts at: 4773 4802 4803 4832 6062 6092 6728 6758 1178
Size: 29 1 29 1230 30 636 30 4039
Fragments arranged by size:
4039 1945 1230 954 636 411 165 30
30 30 30 30 30 29 29 1
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2123 2153 2735 3493 3563 3566 3791 3975
Size: 937 30 582 758 70 3 225 184
Cuts at: 3975 4016 4019 4022 4025 4049 4052 4061 4064
Size: 41 3 3 3 24 3 9 3
Cuts at: 4064 4067 4106 4109 4205 5045 6206 6311 6749
Size: 3 39 3 96 840 1161 105 438
Cuts at: 6749 6794 1186
Size: 45 4011
Fragments arranged by size:
4011 1161 937 840 758 582 438 225
184 105 96 70 45 41 39 30
24 9 3 3 3 3 3 3
3 3
BseYI C'CCAG_C
Cuts at: 2212 3273 3302 3455 3882 4636 4871 4889 4988
Size: 1061 29 153 427 754 235 18 99
Cuts at: 4988 5051 5353 5518 5846 6036 6251 6289 6761
Size: 63 302 165 328 190 215 38 472
Cuts at: 6761 8822 9318 2212
Size: 2061 496 2513
Fragments arranged by size:
2513 2061 1061 754 496 472 427 328
302 235 215 190 165 153 99 63
38 29 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 3086 4463 5564 5747 5882 6461 6877
Size: 573 1350 1377 1101 183 135 579 416
Cuts at: 6877 1163
Size: 3905
Fragments arranged by size:
3905 1377 1350 1101 579 573 416 183
135
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4762 5470 5707 6000 7069 7830
Size: 275 186 2761 708 237 293 1069 761
Cuts at: 7830 8858 1540
Size: 1028 2301
Fragments arranged by size:
2761 2301 1069 1028 761 708 293 275
237 186
BsiHKAI G_wGCw'C
Cuts at: 2496 2670 2739 3255 3465 4401 5148 6492 6571
Size: 174 69 516 210 936 747 1344 79
Cuts at: 6571 7680 7765 8836 2496
Size: 1109 85 1071 3279
Fragments arranged by size:
3279 1344 1109 1071 936 747 516 210
174 85 79 69
BsmI GAATG_Cn'
Cuts at: 7130 7223 7130
Size: 93 9526
BsmBI CGTCTCn'nnnn_
Cuts at: 4708 4822 6278 4708
Size: 114 1456 8049
Fragments arranged by size:
8049 1456 114
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2496 2608 2670 2739 3255 3465 3630
Size: 399 706 112 62 69 516 210 165
Cuts at: 3630 3638 3723 3938 3985 3994 4265 4401 4590
Size: 8 85 215 47 9 271 136 189
Cuts at: 4590 4745 4880 5060 5148 5254 5444 5720 5729
Size: 155 135 180 88 106 190 276 9
Cuts at: 5729 5775 5847 6006 6126 6140 6341 6359 6475
Size: 46 72 159 120 14 201 18 116
Cuts at: 6475 6492 6499 6571 6608 6618 6839 6902 6909
Size: 17 7 72 37 10 221 63 7
Cuts at: 6909 6938 7680 7765 8836 1391
Size: 29 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 706 516 399 276 271
221 215 210 201 190 189 180 165
159 155 136 135 120 116 112 106
88 85 85 72 72 69 63 62
47 46 37 29 18 17 14 10
9 9 8 7 7
BspEI T'CCGG_A
Cuts at: 5950 6448 9282 5950
Size: 498 2834 6287
Fragments arranged by size:
6287 2834 498
BspHI T'CATG_A
Cuts at: 7508 9238 7508
Size: 1730 7889
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2514 2685 2772 2790 2928 3266 4173 4741
Size: 1670 171 87 18 138 338 907 568
Cuts at: 4741 5783 6183 6576 6974 844
Size: 1042 400 393 398 3489
Fragments arranged by size:
3489 1670 1042 907 568 400 398 393
338 171 138 87 18
BsrBI CCG'CTC
Cuts at: 1394 1823 2940 4537 4864 5165 6091 6430 7506
Size: 429 1117 1597 327 301 926 339 1076
Cuts at: 7506 1394
Size: 3507
Fragments arranged by size:
3507 1597 1117 1076 926 429 339 327
301
BsrDI GCAATG_nn'
Cuts at: 65 1248 4245 4647 4710 5640 8114 8288 65
Size: 1183 2997 402 63 930 2474 174 1396
Fragments arranged by size:
2997 2474 1396 1183 930 402 174 63
BsrFI r'CCGG_y
Cuts at: 1700 1810 2055 2712 3239 4430 6457 8263 8450
Size: 110 245 657 527 1191 2027 1806 187
Cuts at: 8450 1700
Size: 2869
Fragments arranged by size:
2869 2027 1806 1191 657 527 245 187
110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 9619
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 9619
BssSI C'ACGA_G
Cuts at: 4403 7679 8691 4403
Size: 3276 1012 5331
Fragments arranged by size:
5331 3276 1012
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2101 2449 3139 3205 3916 5218 5249 5515
Size: 1307 348 690 66 711 1302 31 266
Cuts at: 5515 6232 794
Size: 717 4181
Fragments arranged by size:
4181 1307 1302 717 711 690 348 266
66 31
BstBI TT'CG_AA
Cuts at: 2007 8423 2007
Size: 6416 3203
BstEII G'GTnAC_C
Cuts at: 1184 2482 4489 1184
Size: 1298 2007 6314
Fragments arranged by size:
6314 2007 1298
BstXI CCAn_nnnn'nTGG
Cuts at: 4996 5108 5123 6673 4996
Size: 112 15 1550 7942
Fragments arranged by size:
7942 1550 112 15
BstYI r'GATC_y
Cuts at: 1972 2112 2232 2310 2560 3867 5034 6949 7316
Size: 140 120 78 250 1307 1167 1915 367
Cuts at: 7316 7701 7718 9170 9256 9268 9614 1972
Size: 385 17 1452 86 12 346 1977
Fragments arranged by size:
1977 1915 1452 1307 1167 385 367 346
250 140 120 86 78 17 12
Bsu36I CC'TnA_GG
Cuts at: 3789 4029 5721 6021 3789
Size: 240 1692 300 7387
Fragments arranged by size:
7387 1692 300 240
BtgI C'CryG_G
Cuts at: 513 1065 3723 4113 4410 5150 5368 5445 6889
Size: 552 2658 390 297 740 218 77 1444
Cuts at: 6889 513
Size: 3243
Fragments arranged by size:
3243 2658 1444 740 552 390 297 218
77
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 3351 4831 487
Size: 683 56 2125 1480 5275
Fragments arranged by size:
5275 2125 1480 683 56
BtsI GCAGTG_nn'
Cuts at: 2447 2654 3746 4434 4745 4947 5208 5563 7135
Size: 207 1092 688 311 202 261 355 1572
Cuts at: 7135 7929 7949 2447
Size: 794 20 4117
Fragments arranged by size:
4117 1572 1092 794 688 355 311 261
207 202 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 9619
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 9584
DraI TTT'AAA
Cuts at: 6923 6923
Size: 9619
DraIII CAC_nnn'GTG
Cuts at: 3656 3656
Size: 9619
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4814 8626 809
Size: 507 3498 3812 1802
Fragments arranged by size:
3812 3498 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2256 3063 5160
Size: 54 1425 50 275 18 426 807 2097
Cuts at: 5160 5668 5835 5923 5997 6402 6630 7066 7955
Size: 508 167 88 74 405 228 436 889
Cuts at: 7955 9584 8
Size: 1629 43
Fragments arranged by size:
2097 1629 1425 889 807 508 436 426
405 275 228 167 88 74 54 50
43 18
EagI C'GGCC_G
Cuts at: 1537 1812 5997 7066 1537
Size: 275 4185 1069 4090
Fragments arranged by size:
4185 4090 1069 275
EarI CTCTTCn'nnn_
Cuts at: 1353 3470 3699 4011 4038 4065 4071 4191 4887
Size: 2117 229 312 27 27 6 120 696
Cuts at: 4887 5701 6212 7549 1353
Size: 814 511 1337 3423
Fragments arranged by size:
3423 2117 1337 814 696 511 312 229
120 27 27 6
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3511 8206 8580 8726 387
Size: 1064 138 1922 4695 374 146 1280
Fragments arranged by size:
4695 1922 1280 1064 374 146 138
EcoICRI GAG'CTC
Cuts at: 2494 2668 2737 3463 6490 2494
Size: 174 69 726 3027 5623
Fragments arranged by size:
5623 3027 726 174 69
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2156 2341 2359 2363 2510 2738 2837
Size: 393 524 185 18 4 147 228 99
Cuts at: 2837 3352 3395 4089 4359 4385 4627 5291 5642
Size: 515 43 694 270 26 242 664 351
Cuts at: 5642 5705 5834 5898 5907 6131 6521 6641 6861
Size: 63 129 64 9 224 390 120 220
Cuts at: 6861 7682 8279 9066 9334 1239
Size: 821 597 787 268 1524
Fragments arranged by size:
1524 821 787 694 664 597 524 515
393 390 351 270 268 242 228 224
220 185 147 129 120 99 64 63
43 26 18 9 4
EcoO109I rG'GnC_Cy
Cuts at: 3172 3379 3427 3634 3793 3934 3935 4231 4322
Size: 207 48 207 159 141 1 296 91
Cuts at: 4322 4587 4876 4877 5056 5057 5155 5296 5725
Size: 265 289 1 179 1 98 141 429
Cuts at: 5725 5771 5988 6136 6137 6496 6588 6614 6615
Size: 46 217 148 1 359 92 26 1
Cuts at: 6615 6637 6744 6816 6836 6899 3172
Size: 22 107 72 20 63 5892
Fragments arranged by size:
5892 429 359 296 289 265 217 207
207 179 159 148 141 141 107 98
92 91 72 63 48 46 26 22
20 1 1 1 1 1
EcoRI G'AATT_C
Cuts at: 6928 7306 6928
Size: 378 9241
EcoRV GAT'ATC
Cuts at: 2302 2302
Size: 9619
FspI TGC'GCA
Cuts at: 2207 2405 8125 2207
Size: 198 5720 3701
Fragments arranged by size:
5720 3701 198
HaeII r_GCGC'y
Cuts at: 1678 2050 2686 3002 3439 4395 4421 4603 5853
Size: 372 636 316 437 956 26 182 1250
Cuts at: 5853 6390 6421 6831 6889 8766 1678
Size: 537 31 410 58 1877 2531
Fragments arranged by size:
2531 1877 1250 956 636 537 437 410
372 316 182 58 31 26
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 3515 3547 3623 3655 6062
Size: 32 135 32 1943 32 76 32 2407
Cuts at: 6062 6094 6728 6760 8260 8292 8334 8366 1373
Size: 32 634 32 1500 32 42 32 2626
Fragments arranged by size:
2626 2407 1943 1500 634 135 76 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 6963 7209 669
Size: 988 281 5025 246 3079
Fragments arranged by size:
5025 3079 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 9619
HpaI GTT'AAC
Cuts at: 7209 7209
Size: 9619
KasI G'GCGC_C
Cuts at: 2682 4391 6386 6417 6885 2682
Size: 1709 1995 31 468 5416
Fragments arranged by size:
5416 1995 1709 468 31
KpnI G_GTAC'C
Cuts at: 5873 6944 5873
Size: 1071 8548
MfeI C'AATT_G
Cuts at: 7218 7218
Size: 9619
MluI A'CGCG_T
Cuts at: 9495 9495
Size: 9619
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2685 4839 4989 5059 5112 5475 8733 8917
Size: 1380 2154 150 70 53 363 3258 184
Cuts at: 8917 1305
Size: 2007
Fragments arranged by size:
3258 2154 2007 1380 363 184 150 70
53
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2258 5925 10
Size: 54 1425 343 426 3667 3704
Fragments arranged by size:
3704 3667 1425 426 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 4439 6671 7577 7936 8095 518
Size: 648 3273 2232 906 359 159 2042
Fragments arranged by size:
3273 2232 2042 906 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2041 2164 2239 2272 2361 2401 2557 2647
Size: 435 123 75 33 89 40 156 90
Cuts at: 2647 2815 3416 3823 4504 4832 4922 5152 5172
Size: 168 601 407 681 328 90 230 20
Cuts at: 5172 5370 5548 5885 6076 7035 7712 8860 9105
Size: 198 178 337 191 959 677 1148 245
Cuts at: 9105 1606
Size: 2120
Fragments arranged by size:
2120 1148 959 681 677 601 435 407
337 328 245 230 198 191 178 168
156 123 90 90 89 75 40 33
20
NaeI GCC'GGC
Cuts at: 4432 4432
Size: 9619
NarI GG'CG_CC
Cuts at: 2683 4392 6387 6418 6886 2683
Size: 1709 1995 31 468 5416
Fragments arranged by size:
5416 1995 1709 468 31
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 9067
NdeI CA'TA_TG
Cuts at: 387 387
Size: 9619
NgoMIV G'CCGG_C
Cuts at: 4430 4430
Size: 9619
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 9619
NotI GC'GGCC_GC
Cuts at: 7066 7066
Size: 9619
NspI r_CATG'y
Cuts at: 1132 1229 3919 6977 8522 1132
Size: 97 2690 3058 1545 2229
Fragments arranged by size:
3058 2690 2229 1545 97
PasI CC'CwG_GG
Cuts at: 1348 2944 3978 6324 6376 1348
Size: 1596 1034 2346 52 4591
Fragments arranged by size:
4591 2346 1596 1034 52
PciI A'CATG_T
Cuts at: 8518 8518
Size: 9619
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3373 3527 5051 1108
Size: 2265 154 1524 5676
Fragments arranged by size:
5676 2265 1524 154
PfoI T'CCnGG_A
Cuts at: 2291 2313 2496 3372 4362 5115 6084 2291
Size: 22 183 876 990 753 969 5826
Fragments arranged by size:
5826 990 969 876 753 183 22
PmeI GTTT'AAAC
Cuts at: 6923 6923
Size: 9619
PmlI CAC'GTG
Cuts at: 2062 3106 4606 2062
Size: 1044 1500 7075
Fragments arranged by size:
7075 1500 1044
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 2357 2389 7688 7720 9201 9233 2357
Size: 32 5299 32 1481 32 2743
Fragments arranged by size:
5299 2743 1481 32 32 32
PpuMI rG'GwC_Cy
Cuts at: 5296 6588 6744 5296
Size: 1292 156 8171
Fragments arranged by size:
8171 1292 156
PshAI GACnn'nnGTC
Cuts at: 1613 6715 1613
Size: 5102 4517
PsiI TTA'TAA
Cuts at: 7189 7189
Size: 9619
PspOMI G'GGCC_C
Cuts at: 3634 3934 3990 4586 4876 5056 5725 5771 6136
Size: 300 56 596 290 180 669 46 365
Cuts at: 6136 6495 6614 6835 6898 3634
Size: 359 119 221 63 6355
Fragments arranged by size:
6355 669 596 365 359 300 290 221
180 119 63 56 46
PspXI vC'TCGA_Gb
Cuts at: 2993 3293 2993
Size: 300 9319
PstI C_TGCA'G
Cuts at: 830 2097 2445 3135 3415 3547 3912 4389 4752
Size: 1267 348 690 280 132 365 477 363
Cuts at: 4752 5808 6061 6971 830
Size: 1056 253 910 3478
Fragments arranged by size:
3478 1267 1056 910 690 477 365 363
348 280 253 132
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 9619
PvuII CAG'CTG
Cuts at: 2164 2272 2401 2557 2647 2815 3416 5548 5885
Size: 108 129 156 90 168 601 2132 337
Cuts at: 5885 6076 2164
Size: 191 5707
Fragments arranged by size:
5707 2132 601 337 191 168 156 129
108 90
RsrII CG'GwC_CG
Cuts at: 3237 3237
Size: 9619
SacI G_AGCT'C
Cuts at: 2496 2670 2739 3465 6492 2496
Size: 174 69 726 3027 5623
Fragments arranged by size:
5623 3027 726 174 69
SacII CC_GC'GG
Cuts at: 5153 5371 5153
Size: 218 9401
SalI G'TCGA_C
Cuts at: 1936 6961 1936
Size: 5025 4594
SanDI GG'GwC_CC
Cuts at: 5296 5296
Size: 9619
SapI GCTCTTCn'nnn_
Cuts at: 4191 4191
Size: 9619
SbfI CC_TGCA'GG
Cuts at: 6971 6971
Size: 9619
ScaI AGT'ACT
Cuts at: 6101 7867 9579 6101
Size: 1766 1712 6141
Fragments arranged by size:
6141 1766 1712
SexAI A'CCwGG_T
Cuts at: 2478 2478
Size: 9619
SfcI C'TryA_G
Cuts at: 826 1045 2093 2122 2441 3131 3411 3431 3543
Size: 219 1048 29 319 690 280 20 112
Cuts at: 3543 3908 4385 4675 4748 5804 5983 6057 6209
Size: 365 477 290 73 1056 179 74 152
Cuts at: 6209 6967 8102 8783 8974 826
Size: 758 1135 681 191 1471
Fragments arranged by size:
1471 1135 1056 1048 758 690 681 477
365 319 290 280 219 191 179 152
112 74 73 29 20
SfiI GGCCn_nnn'nGGCC
Cuts at: 5987 5996 6503 5987
Size: 9 507 9103
Fragments arranged by size:
9103 507 9
SfoI GGC'GCC
Cuts at: 2684 4393 6388 6419 6887 2684
Size: 1709 1995 31 468 5416
Fragments arranged by size:
5416 1995 1709 468 31
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 9619
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 9619
SmaI CCC'GGG
Cuts at: 1872 2455 3757 3949 4334 4585 4960 5310 6708
Size: 583 1302 192 385 251 375 350 1398
Cuts at: 6708 6946 1872
Size: 238 4545
Fragments arranged by size:
4545 1398 1302 583 385 375 350 251
238 192
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2027 2510 2993 3046 3167 3293
Size: 197 926 84 483 483 53 121 126
Cuts at: 3293 3464 6346 7723 8624 8886 820
Size: 171 2882 1377 901 262 1553
Fragments arranged by size:
2882 1553 1377 926 901 483 483 262
197 171 126 121 84 53
SnaBI TAC'GTA
Cuts at: 493 6916 7410 493
Size: 6423 494 2702
Fragments arranged by size:
6423 2702 494
SpeI A'CTAG_T
Cuts at: 152 152
Size: 9619
SphI G_CATG'C
Cuts at: 1132 3919 6977 1132
Size: 2787 3058 3774
Fragments arranged by size:
3774 3058 2787
SrfI GCCC'GGGC
Cuts at: 4334 4334
Size: 9619
SspI AAT'ATT
Cuts at: 5 52 7543 5
Size: 47 7491 2081
Fragments arranged by size:
7491 2081 47
StuI AGG'CCT
Cuts at: 1517 3506 5981 6880 1517
Size: 1989 2475 899 4256
Fragments arranged by size:
4256 2475 1989 899
StyI C'CwwG_G
Cuts at: 513 1065 5139 5433 6682 7058 513
Size: 552 4074 294 1249 376 3074
Fragments arranged by size:
4074 3074 1249 552 376 294
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2798 3170 4340 6152 7646 7663 7816
Size: 1255 1218 372 1170 1812 1494 17 153
Cuts at: 7816 8001 8446 325
Size: 185 445 1498
Fragments arranged by size:
1812 1498 1494 1255 1218 1170 445 372
185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 6099 7865 9577
Size: 274 80 33 51 1443 4122 1766 1712
Cuts at: 9577 96
Size: 138
Fragments arranged by size:
4122 1766 1712 1443 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 3021 3053 4332 4364 4773 4802 4805 4834 6561
Size: 32 1279 32 409 29 3 29 1727
Cuts at: 6561 6593 9579 9611 3021
Size: 32 2986 32 3029
Fragments arranged by size:
3029 2986 1727 1279 409 32 32 32
32 29 29 3
Tth111I GACn'n_nGTC
Cuts at: 1316 4763 1316
Size: 3447 6172
XbaI T'CTAG_A
Cuts at: 6955 6955
Size: 9619
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2974 3524 5339 2974
Size: 550 1815 7254
Fragments arranged by size:
7254 1815 550
XhoI C'TCGA_G
Cuts at: 1943 2993 3293 1943
Size: 1050 300 8269
Fragments arranged by size:
8269 1050 300
XmaI C'CCGG_G
Cuts at: 1870 2453 3755 3947 4332 4583 4958 5308 6706
Size: 583 1302 192 385 251 375 350 1398
Cuts at: 6706 6944 1870
Size: 238 4545
Fragments arranged by size:
4545 1398 1302 583 385 375 350 251
238 192
XmnI GAAnn'nnTTC
Cuts at: 7748 7748
Size: 9619
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 7427 276
Size: 53 83 186 936 5893 2468
Fragments arranged by size:
5893 2468 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI
ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII
BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII
BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI
BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI
EarI EciI EcoICRI Eco57MI EcoO109I EcoRI EcoRV FspI
HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI MslI MspA1I NaeI NarI NcoI
NdeI NgoMIV NheI NotI NspI PasI PciI PflMI
PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI
PspXI PstI PvuI PvuII RsrII SacI SacII SalI
SanDI SapI SbfI ScaI SexAI SfcI SfiI SfoI
SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI
SspI StuI StyI TaqII TatI TstI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AscI AvrII BaeI BfrBI BsiWI BstZ17I EcoNI FalI
FseI FspAI NruI NsiI PacI PsrI SwaI