Restriction map for
pFN21ASDA0146
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# NO CUT ENZYMES ( 85 enzymes) #
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AatII AccI AclI AgeI AhdI
Aor51HI ApaI AscI AseI AsnI
AspEI AviII AvrII BamHI BciVI
BclI BglI BlnI BlpI Bpu1102I
BsaBI BsiEI BsiWI BsmBI Bsp1407I
BspDI BspHI BsrDI BsrGI BssHII
Bst1107I BstBI BstXI BstZ17I CelII
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HpaI MamI MfeI MluI MslI
MunI NaeI NdeI NgoMIV NheI
NotI NruI NsiI NspV PacI
PinAI PmeI PpuMI PshBI Psp1406I
PvuI RcaI RsrII SalI ScaI
SexAI SfiI SfuI SnaBI SpeI
SphI SspBI SspI SwaI XbaI
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# ONE CUT ENZYMES ( 66 enzymes) #
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Acc65I 1930 GGTACC G^GTACC
AcyI 2437 GGCGCC G[GA]^CG[CT]C
AflII 1899 CTTAAG C^TTAAG
AflIII 2393 ACATGT A^C[GA][CT]GT
Alw44I 1588 GTGCAC G^TGCAC
AlwNI 1433 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 1588 GTGCAC G^TGCAC
Asp700I 160 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
Asp718I 1930 GGTACC G^GTACC
AspI 1072 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaII 1331 GGACC G^G[AT]CC
BalI 1429 TGGCCA TGG^CCA
BbeI 2437 GGCGCC GGCGC^C
BbrPI 625 CACGTG CAC^GTG
BbvCI 2577 GCTGAGG CCTCAGC(-5/-2)
BcgI 2361 CGAGAGCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BfrI 1899 CTTAAG C^TTAAG
BsaAI 625 CACGTG [CT]AC^GT[GA]
BsaHI 2437 GGCGCC G[GA]^CG[CT]C
BsmI 55 GAATGC GAATGC(1/-1)
BspLU11I 2393 ACATGT A^CATGT
BsrBI 2517 CCGCTC CCGCTC(-3/-3)
BsrFI 2590 GCCGGT [GA]^CCGG[CT]
BssSI 1425 CTCGTG CACGAG(-5/-1)
BstAPI 637 GCATCGGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstEII 965 GGTCACC G^GT[ACGT]ACC
BstPI 965 GGTCACC G^GT[ACGT]ACC
Cfr10I 2590 GCCGGT [GA]^CCGG[CT]
DraII 2728 GGGGCCT [GA]G^G[ACGT]CC[CT]
DraIII 1713 CACCCTGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoNI 2625 CCTCGGAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 2728 GGGGCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 965 GGTCACC G^GT[ACGT]ACC
HaeII 2437 GGCGCC [GA]GCGC^[CT]
HgaI 2170 GACGC GACGC(5/10)
Hin1I 2437 GGCGCC G[GA]^CG[CT]C
HincII 2317 GTCAAC GT[CT]^[GA]AC
HindII 2317 GTCAAC GT[CT]^[GA]AC
KasI 2437 GGCGCC G^GCGCC
KpnI 1930 GGTACC GGTAC^C
KspI 6 CCGCGG CCGC^GG
MaeII 626 ACGT A^CGT
MluNI 1429 TGGCCA TGG^CCA
MscI 1429 TGGCCA TGG^CCA
NarI 2437 GGCGCC GG^CGCC
NcoI 1335 CCATGG C^CATGG
NspI 2393 ACATGT [GA]CATG^[CT]
PaeR7I 1541 CTCGAG C^TCGAG
PflFI 1072 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PmaCI 625 CACGTG CAC^GTG
PmlI 625 CACGTG CAC^GTG
SacI 303 GAGCTC GAGCT^C
SacII 6 CCGCGG CCGC^GG
SapI 95 GAAGAGC GCTCTTC(1/4)
SbfI 2496 CCTGCAGG CCTGCA^GG
SfoI 2437 GGCGCC GGC^GCC
SgrAI 2589 CGCCGGTG C[GA]^CCGG[CT]G
SmaI 2462 CCCGGG CCC^GGG
Sse8387I 2496 CCTGCAGG CCTGCA^GG
StuI 1003 AGGCCT AGG^CCT
Tth111I 1072 GACACTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XcmI 2072 CCAAAACCCTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoI 1541 CTCGAG C^TCGAG
XmaCI 2462 CCCGGG C^CCGGG
XmaI 2462 CCCGGG C^CCGGG
XmnI 160 GAAGGGTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 15 enzymes) #
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AccIII 1079 TCCGGA T^CCGGA
1206 TCCGGA
AlwI 673 GATCC GGATC(4/5)
1082 GGATC
AocI 1053 CCTCAGG CC^T[ACGT]AGG
1380 CCTGAGG
BglII 820 AGATCT A^GATCT
2009 AGATCT
BsaWI 1079 TCCGGA [AT]^CCGG[AT]
1206 TCCGGA
BseAI 1079 TCCGGA T^CCGGA
1206 TCCGGA
BspEI 1079 TCCGGA T^CCGGA
1206 TCCGGA
Bsu36I 1053 CCTCAGG CC^T[ACGT]AGG
1380 CCTGAGG
EaeI 1429 TGGCCA [CT]^GGCC[GA]
1749 TGGCCG
Eco81I 1053 CCTCAGG CC^T[ACGT]AGG
1380 CCTGAGG
HindIII 766 AAGCTT A^AGCTT
1870 AAGCTT
MroI 1079 TCCGGA T^CCGGA
1206 TCCGGA
PflMI 987 CCAAAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1432 CCAGGAGCTGG
PshAI 2025 GACCGATGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
2182 GACCAGGGTC
Van91I 987 CCAAAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
1432 CCAGGAGCTGG
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# 3 OR MORE CUT ENZYMES ( 92 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
ApoI AspHI AvaI BanI BanII
BbsI BbvI BcnI BfaI BmyI
BpmI BpuAI BsaI BsaJI BseRI
BsgI BsiHKAI BsiYI BslI BsmAI
BsmFI BsoBI Bsp1286I BspMI BsrI
BssKI BstF5I BstNI BstUI BstYI
Cac8I CfoI Cfr13I DdeI DpnII
DsaI EarI EcoRII EcoT14I FauI
Fnu4HI FokI HaeIII HapII HhaI
HinP1I HinfI HpaII HphI ItaI
Ksp632I MaeI MaeIII MboI MboII
MflI MnlI MseI MspA1I MspI
MvaI MvnI MwoI NciI NdeII
NlaIII NlaIV PleI PstI PvuII
RsaI Sau3AI Sau96I ScrFI SfaNI
SfcI SmlI StyI TaqI TfiI
Tru9I TseI Tsp45I Tsp509I TspRI
TthHB8I XhoII