(Circular) (Six-base) MAPSORT of: pFN21ASDA0146.seq Check: 868 from: 1 to: 7474 pFN21ASDA0146 7474 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 4527 4527 Size: 7474 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5284 278 Size: 53 83 186 936 3748 2468 Fragments arranged by size: 3748 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4817 1937 Size: 2880 4594 Acc65I G'GTAC_C Cuts at: 3953 4795 3953 Size: 842 6632 AclI AA'CG_TT Cuts at: 1563 5601 5974 1563 Size: 4038 373 3063 Fragments arranged by size: 4038 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2181 2244 4022 4161 4232 5537 6921 7189 Size: 549 63 1778 139 71 1305 1384 268 Cuts at: 7189 1632 Size: 1917 Fragments arranged by size: 1917 1778 1384 1305 549 268 139 71 63 AflII C'TTAA_G Cuts at: 820 1017 3922 820 Size: 197 2905 4372 Fragments arranged by size: 4372 2905 197 AflIII A'CryG_T Cuts at: 1203 4416 6373 7350 1203 Size: 3213 1957 977 1327 Fragments arranged by size: 3213 1957 1327 977 AgeI A'CCGG_T Cuts at: 6305 6305 Size: 7474 AhdI GACnn_n'nnGTC Cuts at: 6203 6203 Size: 7474 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7474 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7474 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3666 3698 4221 4253 3666 Size: 32 523 32 6887 Fragments arranged by size: 6887 523 32 32 AlwNI CAG_nnn'CTG Cuts at: 3461 5217 6789 3461 Size: 1756 1572 4146 Fragments arranged by size: 4146 1756 1572 ApaLI G'TGCA_C Cuts at: 3611 5531 6687 3611 Size: 1920 1156 4398 Fragments arranged by size: 4398 1920 1156 ApoI r'AATT_y Cuts at: 1493 2447 2562 2773 2928 4783 5009 5161 7270 Size: 954 115 211 155 1855 226 152 2109 Cuts at: 7270 1493 Size: 1697 Fragments arranged by size: 2109 1855 1697 954 226 211 155 152 115 AseI AT'TA_AT Cuts at: 160 1032 6028 160 Size: 872 4996 1606 Fragments arranged by size: 4996 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7474 AvaI C'yCGr_G Cuts at: 1870 1943 2043 3564 4485 4799 1870 Size: 73 100 1521 921 314 4545 Fragments arranged by size: 4545 1521 921 314 100 73 BamHI G'GATC_C Cuts at: 4804 5171 4804 Size: 367 7107 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3953 4460 4515 4795 6250 Size: 325 217 627 2166 507 55 280 1455 Cuts at: 6250 618 Size: 1842 Fragments arranged by size: 2166 1842 1455 627 507 325 280 217 55 BanII G_rGCy'C Cuts at: 1391 2051 2330 4139 4793 1391 Size: 660 279 1809 654 4072 Fragments arranged by size: 4072 1809 660 654 279 BbeI G_GCGC'C Cuts at: 4464 4464 Size: 7474 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2237 3885 4458 4519 928 Size: 971 338 1648 573 61 3883 Fragments arranged by size: 3883 1648 971 573 338 61 BbvCI CC'TCA_GC Cuts at: 4601 4601 Size: 7474 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4373 4407 5665 5699 1903 Size: 34 2436 34 1258 34 3678 Fragments arranged by size: 3678 2436 1258 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5366 6576 5366 Size: 1210 6264 BclI T'GATC_A Cuts at: 1548 1548 Size: 7474 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6085 136 Size: 107 122 71 631 605 4413 1525 Fragments arranged by size: 4413 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2843 4032 7469 2843 Size: 1189 3437 2848 Fragments arranged by size: 3437 2848 1189 BlpI GC'TnA_GC Cuts at: 4891 4891 Size: 7474 Bme1580I G_kGCm'C Cuts at: 1790 3366 3615 5535 6691 1790 Size: 1576 249 1920 1156 2573 Fragments arranged by size: 2573 1920 1576 1156 249 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7474 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2413 5205 6163 Size: 416 53 83 399 24 996 2792 958 Cuts at: 6163 442 Size: 1753 Fragments arranged by size: 2792 1753 996 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7474 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2144 3429 3950 2144 Size: 1285 521 5668 Fragments arranged by size: 5668 1285 521 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2314 2751 3051 3483 3801 3891 3920 4040 Size: 1075 437 300 432 318 90 29 120 Cuts at: 4040 4169 6134 1239 Size: 129 1965 2579 Fragments arranged by size: 2579 1965 1075 437 432 318 300 129 120 90 29 Bpu10I CC'TnA_GC Cuts at: 2171 3538 4601 2171 Size: 1367 1063 5044 Fragments arranged by size: 5044 1367 1063 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4493 4528 5599 6464 6762 4493 Size: 35 1071 865 298 5205 Fragments arranged by size: 5205 1071 865 298 35 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2132 2711 4074 4276 6137 882 Size: 617 633 579 1363 202 1861 2219 Fragments arranged by size: 2219 1861 1363 633 617 579 202 BsaAI yAC'GTr Cuts at: 493 1204 2650 4771 5265 493 Size: 711 1446 2121 494 2702 Fragments arranged by size: 2702 2121 1446 711 494 BsaBI GATnn'nnATC Cuts at: 7468 7468 Size: 7474 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4461 5281 5663 6298 Size: 53 83 186 936 2928 820 382 635 Cuts at: 6298 275 Size: 1451 Fragments arranged by size: 2928 1451 936 820 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3102 3229 5907 6305 6579 6726 7137 3102 Size: 127 2678 398 274 147 411 3439 Fragments arranged by size: 3439 2678 411 398 274 147 127 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2317 2347 3382 3412 3450 Size: 30 165 30 914 30 1035 30 38 Cuts at: 3450 3456 3480 3486 1178 Size: 6 24 6 5166 Fragments arranged by size: 5166 1035 914 165 38 30 30 30 30 24 6 6 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2995 3804 4088 1186 Size: 1809 809 284 4572 Fragments arranged by size: 4572 1809 809 284 BseYI C'CCAG_C Cuts at: 3500 3553 3748 4132 4160 6677 7173 3500 Size: 53 195 384 28 2517 496 3801 Fragments arranged by size: 3801 2517 496 384 195 53 28 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2672 4576 4764 1163 Size: 573 936 1904 188 3873 Fragments arranged by size: 3873 1904 936 573 188 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4924 5685 6713 1540 Size: 275 186 2923 761 1028 2301 Fragments arranged by size: 2923 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2330 3615 4391 5535 5620 6691 2330 Size: 1285 776 1144 85 1071 3113 Fragments arranged by size: 3113 1285 1144 1071 776 85 BsmI GAATG_Cn' Cuts at: 2084 4985 5078 2084 Size: 2901 93 4480 Fragments arranged by size: 4480 2901 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2051 2330 3366 3615 4139 4391 4793 Size: 399 261 279 1036 249 524 252 402 Cuts at: 4793 5535 5620 6691 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 1036 742 524 402 399 279 261 252 249 85 BspEI T'CCGG_A Cuts at: 3102 3229 7137 3102 Size: 127 3908 3439 Fragments arranged by size: 3908 3439 127 BspHI T'CATG_A Cuts at: 5363 7093 5363 Size: 1730 5744 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2101 2114 4513 4527 4829 844 Size: 1257 13 2399 14 302 3489 Fragments arranged by size: 3489 2399 1257 302 14 13 BsrBI CCG'CTC Cuts at: 1394 1823 4542 5361 1394 Size: 429 2719 819 3507 Fragments arranged by size: 3507 2719 819 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5969 6143 65 Size: 1183 4721 174 1396 Fragments arranged by size: 4721 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4613 6118 6305 1700 Size: 110 2803 1505 187 2869 Fragments arranged by size: 2869 2803 1505 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7474 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7474 BssSI C'ACGA_G Cuts at: 3448 5534 6546 3448 Size: 2086 1012 4376 Fragments arranged by size: 4376 2086 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2666 794 Size: 1872 5602 BstBI TT'CG_AA Cuts at: 2007 6278 2007 Size: 4271 3203 BstEII G'GTnAC_C Cuts at: 1184 2988 1184 Size: 1804 5670 BstYI r'GATC_y Cuts at: 1972 2695 2843 3105 4032 4804 5171 5556 5573 Size: 723 148 262 927 772 367 385 17 Cuts at: 5573 7025 7111 7123 7469 1972 Size: 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 927 772 723 385 367 346 262 148 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3077 3404 3077 Size: 327 7147 BtgI C'CryG_G Cuts at: 513 1065 2029 3358 3975 513 Size: 552 964 1329 617 4012 Fragments arranged by size: 4012 1329 964 617 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6735 Fragments arranged by size: 6735 683 56 BtsI GCAGTG_nn' Cuts at: 2105 4360 4389 4559 4990 5784 5804 2105 Size: 2255 29 170 431 794 20 3775 Fragments arranged by size: 3775 2255 794 431 170 29 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7474 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7439 DraI TTT'AAA Cuts at: 4778 4778 Size: 7474 DraIII CAC_nnn'GTG Cuts at: 3741 3741 Size: 7474 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6481 809 Size: 507 5165 1802 Fragments arranged by size: 5165 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3452 3772 4921 Size: 54 1425 50 275 18 1622 320 1149 Cuts at: 4921 5810 7439 8 Size: 889 1629 43 Fragments arranged by size: 1629 1622 1425 1149 889 320 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4921 1537 Size: 275 3109 4090 Fragments arranged by size: 4090 3109 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2113 2220 3056 3501 3786 3998 4187 4634 Size: 760 107 836 445 285 212 189 447 Cuts at: 4634 5404 1353 Size: 770 3423 Fragments arranged by size: 3423 836 770 760 447 445 285 212 189 107 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 6061 6435 6581 387 Size: 1064 138 4472 374 146 1280 Fragments arranged by size: 4472 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2328 2328 Size: 7474 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2181 2244 2314 2751 3051 3483 3801 Size: 393 549 63 70 437 300 432 318 Cuts at: 3801 3891 3920 4022 4040 4161 4169 4232 5537 Size: 90 29 102 18 121 8 63 1305 Cuts at: 5537 6134 6921 7189 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 1305 787 597 549 437 432 393 318 300 268 121 102 90 70 63 63 29 18 8 EcoNI CCTnn'n_nnAGG Cuts at: 4652 4652 Size: 7474 EcoO109I rG'GnC_Cy Cuts at: 4752 4752 Size: 7474 EcoRI G'AATT_C Cuts at: 4783 5161 4783 Size: 378 7096 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3276 3276 Size: 7474 FspI TGC'GCA Cuts at: 5980 5980 Size: 7474 HaeII r_GCGC'y Cuts at: 1678 4464 6621 1678 Size: 2786 2157 2531 Fragments arranged by size: 2786 2531 2157 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2353 2385 2516 2548 2561 Size: 32 135 32 781 32 131 32 13 Cuts at: 2561 2593 2615 2647 3097 3129 3450 3482 3787 Size: 32 22 32 450 32 321 32 305 Cuts at: 3787 3819 3931 3963 4526 4551 4558 4583 6115 Size: 32 112 32 563 25 7 25 1532 Cuts at: 6115 6147 6189 6221 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1532 781 563 450 321 305 135 131 112 42 32 32 32 32 32 32 32 32 32 32 32 32 25 25 22 13 7 HincII GTy'rAC Cuts at: 669 1657 1938 4342 4818 5064 669 Size: 988 281 2404 476 246 3079 Fragments arranged by size: 3079 2404 988 476 281 246 HindIII A'AGCT_T Cuts at: 748 2789 3893 748 Size: 2041 1104 4329 Fragments arranged by size: 4329 2041 1104 HpaI GTT'AAC Cuts at: 5064 5064 Size: 7474 KasI G'GCGC_C Cuts at: 4460 4460 Size: 7474 KpnI G_GTAC'C Cuts at: 3957 4799 3957 Size: 842 6632 MfeI C'AATT_G Cuts at: 5073 5073 Size: 7474 MluI A'CGCG_T Cuts at: 7350 7350 Size: 7474 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2048 2328 2514 3627 4193 6588 6772 1305 Size: 743 280 186 1113 566 2395 184 2007 Fragments arranged by size: 2395 2007 1113 743 566 280 186 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3454 10 Size: 54 1425 343 1622 4030 Fragments arranged by size: 4030 1622 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 5432 5791 5950 518 Size: 648 4266 359 159 2042 Fragments arranged by size: 4266 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2031 2147 2222 3557 3713 3918 4000 4710 Size: 425 116 75 1335 156 205 82 710 Cuts at: 4710 4890 5567 6715 6960 1606 Size: 180 677 1148 245 2120 Fragments arranged by size: 2120 1335 1148 710 677 425 245 205 180 156 116 82 75 NarI GG'CG_CC Cuts at: 4461 4461 Size: 7474 NcoI C'CATG_G Cuts at: 513 1065 3358 513 Size: 552 2293 4629 Fragments arranged by size: 4629 2293 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7474 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7474 NotI GC'GGCC_GC Cuts at: 4921 4921 Size: 7474 NspI r_CATG'y Cuts at: 1132 1229 4420 4832 6377 1132 Size: 97 3191 412 1545 2229 Fragments arranged by size: 3191 2229 1545 412 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 7474 PciI A'CATG_T Cuts at: 4416 6373 4416 Size: 1957 5517 PflMI CCAn_nnn'nTGG Cuts at: 1108 3016 3461 1108 Size: 1908 445 5121 Fragments arranged by size: 5121 1908 445 PfoI T'CCnGG_A Cuts at: 2092 3417 4146 4530 2092 Size: 1325 729 384 5036 Fragments arranged by size: 5036 1325 729 384 PmeI GTTT'AAAC Cuts at: 4778 4778 Size: 7474 PmlI CAC'GTG Cuts at: 2650 2650 Size: 7474 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5543 5575 7056 7088 5543 Size: 32 1481 32 5929 Fragments arranged by size: 5929 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 4052 4209 1613 Size: 2439 157 4878 Fragments arranged by size: 4878 2439 157 PsiI TTA'TAA Cuts at: 3891 5044 3891 Size: 1153 6321 PspXI vC'TCGA_Gb Cuts at: 3564 3564 Size: 7474 PstI C_TGCA'G Cuts at: 830 2112 2832 3070 4355 4524 4762 4826 830 Size: 1282 720 238 1285 169 238 64 3478 Fragments arranged by size: 3478 1285 1282 720 238 238 169 64 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7474 PvuII CAG'CTG Cuts at: 2147 2222 3557 4000 4710 2147 Size: 75 1335 443 710 4911 Fragments arranged by size: 4911 1335 710 443 75 SacI G_AGCT'C Cuts at: 2330 2330 Size: 7474 SacII CC_GC'GG Cuts at: 2032 2032 Size: 7474 SalI G'TCGA_C Cuts at: 1936 4816 1936 Size: 2880 4594 SapI GCTCTTCn'nnn_ Cuts at: 2113 2113 Size: 7474 SbfI CC_TGCA'GG Cuts at: 4524 4826 4524 Size: 302 7172 ScaI AGT'ACT Cuts at: 5722 7434 5722 Size: 1712 5762 SfcI C'TryA_G Cuts at: 826 1045 2108 2556 2828 3066 3339 3686 4351 Size: 219 1063 448 272 238 273 347 665 Cuts at: 4351 4520 4758 4822 5957 6638 6829 826 Size: 169 238 64 1135 681 191 1471 Fragments arranged by size: 1471 1135 1063 681 665 448 347 273 272 238 238 219 191 169 64 SfoI GGC'GCC Cuts at: 4462 4462 Size: 7474 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7474 SgrAI Cr'CCGG_yG Cuts at: 1700 4613 1700 Size: 2913 4561 SmaI CCC'GGG Cuts at: 1872 4487 4801 1872 Size: 2615 314 4545 Fragments arranged by size: 4545 2615 314 SmlI C'TyrA_G Cuts at: 820 1017 1943 3564 3922 4508 4543 5578 6479 Size: 197 926 1621 358 586 35 1035 901 Cuts at: 6479 6741 820 Size: 262 1553 Fragments arranged by size: 1621 1553 1035 926 901 586 358 262 197 35 SnaBI TAC'GTA Cuts at: 493 4771 5265 493 Size: 4278 494 2702 Fragments arranged by size: 4278 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7474 SphI G_CATG'C Cuts at: 1132 4832 1132 Size: 3700 3774 SspI AAT'ATT Cuts at: 5 52 5398 5 Size: 47 5346 2081 Fragments arranged by size: 5346 2081 47 StuI AGG'CCT Cuts at: 1517 3028 1517 Size: 1511 5963 StyI C'CwwG_G Cuts at: 513 1065 2699 3057 3358 4913 513 Size: 552 1634 358 301 1555 3074 Fragments arranged by size: 3074 1634 1555 552 358 301 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4064 5501 5518 5671 5856 6301 325 Size: 1255 2484 1437 17 153 185 445 1498 Fragments arranged by size: 2484 1498 1437 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3222 5720 7432 Size: 274 80 33 51 1443 1245 2498 1712 Cuts at: 7432 96 Size: 138 Fragments arranged by size: 2498 1712 1443 1245 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2317 2349 3294 3326 3456 3488 3829 3861 4207 Size: 32 945 32 130 32 341 32 346 Cuts at: 4207 4239 4644 4676 7434 7466 2317 Size: 32 405 32 2758 32 2325 Fragments arranged by size: 2758 2325 945 405 346 341 130 32 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3098 1316 Size: 1782 5692 XbaI T'CTAG_A Cuts at: 4810 4810 Size: 7474 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4102 4102 Size: 7474 XhoI C'TCGA_G Cuts at: 1943 3564 1943 Size: 1621 5853 XmaI C'CCGG_G Cuts at: 1870 4485 4799 1870 Size: 2615 314 4545 Fragments arranged by size: 4545 2615 314 XmnI GAAnn'nnTTC Cuts at: 2187 5603 2187 Size: 3416 4058 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5282 276 Size: 53 83 186 936 3748 2468 Fragments arranged by size: 3748 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PshAI PsiI PspXI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BfrBI BsiWI BsmBI BstXI BstZ17I EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PpuMI PspOMI PsrI RsrII SanDI SexAI SfiI SrfI SwaI