Restriction map for
pFN21ASDA0146
(Linear) (Six-base) MAPSORT of: KIAA0146.seq Check: 4962 from: 1 to: 2745
KIAA0146.orf
With 182 enzymes: SgfI *
October 24, 2006 15:11 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 2504 2745
Size: 2504 241
Acc65I G'GTAC_C
Cuts at: 0 1930 2745
Size: 1930 815
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 221 1999 2138 2209 2745
Size: 158 63 1778 139 71 536
Fragments arranged by size:
1778 536 158 139 71 63
AflII C'TTAA_G
Cuts at: 0 1899 2745
Size: 1899 846
AflIII A'CryG_T
Cuts at: 0 2393 2745
Size: 2393 352
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1643 1675 2198 2230 2745
Size: 1643 32 523 32 515
Fragments arranged by size:
1643 523 515 32 32
AlwNI CAG_nnn'CTG
Cuts at: 0 1438 2745
Size: 1438 1307
ApaLI G'TGCA_C
Cuts at: 0 1588 2745
Size: 1588 1157
ApoI r'AATT_y
Cuts at: 0 424 539 750 905 2745
Size: 424 115 211 155 1840
Fragments arranged by size:
1840 424 211 155 115
AvaI C'yCGr_G
Cuts at: 0 20 1541 2462 2745
Size: 20 1521 921 283
Fragments arranged by size:
1521 921 283 20
BanI G'GyrC_C
Cuts at: 0 1930 2437 2492 2745
Size: 1930 507 55 253
Fragments arranged by size:
1930 507 253 55
BanII G_rGCy'C
Cuts at: 0 28 307 2116 2745
Size: 28 279 1809 629
Fragments arranged by size:
1809 629 279 28
BbeI G_GCGC'C
Cuts at: 0 2441 2745
Size: 2441 304
BbsI GAAGACnn'nnnn_
Cuts at: 0 214 1862 2435 2496 2745
Size: 214 1648 573 61 249
Fragments arranged by size:
1648 573 249 214 61
BbvCI CC'TCA_GC
Cuts at: 0 2578 2745
Size: 2578 167
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2350 2384 2745
Size: 2350 34 361
Fragments arranged by size:
2350 361 34
BglII A'GATC_T
Cuts at: 0 820 2009 2745
Size: 820 1189 736
Fragments arranged by size:
1189 820 736
Bme1580I G_kGCm'C
Cuts at: 0 1343 1592 2745
Size: 1343 249 1153
Fragments arranged by size:
1343 1153 249
BmrI ACTGGGnnnn_n'
Cuts at: 0 390 2745
Size: 390 2355
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 121 1406 1927 2745
Size: 121 1285 521 818
Fragments arranged by size:
1285 818 521 121
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 291 728 1028 1460 1778 1868 1897 2017
Size: 291 437 300 432 318 90 29 120
Cuts at: 2017 2146 2745
Size: 129 599
Fragments arranged by size:
599 437 432 318 300 291 129 120
90 29
Bpu10I CC'TnA_GC
Cuts at: 0 148 1515 2578 2745
Size: 148 1367 1063 167
Fragments arranged by size:
1367 1063 167 148
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 2470 2505 2745
Size: 2470 35 240
Fragments arranged by size:
2470 240 35
BsaI GGTCTCn'nnnn_
Cuts at: 0 109 688 2051 2253 2745
Size: 109 579 1363 202 492
Fragments arranged by size:
1363 579 492 202 109
BsaAI yAC'GTr
Cuts at: 0 627 2745
Size: 627 2118
BsaHI Gr'CG_yC
Cuts at: 0 2438 2745
Size: 2438 307
BsaWI w'CCGG_w
Cuts at: 0 1079 1206 2745
Size: 1079 127 1539
Fragments arranged by size:
1539 1079 127
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 294 324 1359 1389 1427 1433 1457 1463
Size: 294 30 1035 30 38 6 24 6
Cuts at: 1463 2745
Size: 1282
Fragments arranged by size:
1282 1035 294 38 30 30 24 6
6
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 972 1781 2065 2745
Size: 972 809 284 680
Fragments arranged by size:
972 809 680 284
BseYI C'CCAG_C
Cuts at: 0 1477 1530 1725 2109 2137 2745
Size: 1477 53 195 384 28 608
Fragments arranged by size:
1477 608 384 195 53 28
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 649 2553 2741 2745
Size: 649 1904 188 4
Fragments arranged by size:
1904 649 188 4
BsiHKAI G_wGCw'C
Cuts at: 0 307 1592 2368 2745
Size: 307 1285 776 377
Fragments arranged by size:
1285 776 377 307
BsmI GAATG_Cn'
Cuts at: 0 61 2745
Size: 61 2684
Bsp1286I G_dGCh'C
Cuts at: 0 28 307 1343 1592 2116 2368 2745
Size: 28 279 1036 249 524 252 377
Fragments arranged by size:
1036 524 377 279 252 249 28
BspEI T'CCGG_A
Cuts at: 0 1079 1206 2745
Size: 1079 127 1539
Fragments arranged by size:
1539 1079 127
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 78 91 2490 2504 2745
Size: 78 13 2399 14 241
Fragments arranged by size:
2399 241 78 14 13
BsrBI CCG'CTC
Cuts at: 0 2519 2745
Size: 2519 226
BsrFI r'CCGG_y
Cuts at: 0 2590 2745
Size: 2590 155
BssSI C'ACGA_G
Cuts at: 0 1425 2745
Size: 1425 1320
BstAPI GCAn_nnn'nTGC
Cuts at: 0 643 2745
Size: 643 2102
BstEII G'GTnAC_C
Cuts at: 0 965 2745
Size: 965 1780
BstYI r'GATC_y
Cuts at: 0 672 820 1082 2009 2745
Size: 672 148 262 927 736
Fragments arranged by size:
927 736 672 262 148
Bsu36I CC'TnA_GG
Cuts at: 0 1054 1381 2745
Size: 1054 327 1364
Fragments arranged by size:
1364 1054 327
BtgI C'CryG_G
Cuts at: 0 6 1335 1952 2745
Size: 6 1329 617 793
Fragments arranged by size:
1329 793 617 6
BtsI GCAGTG_nn'
Cuts at: 0 82 2337 2366 2536 2745
Size: 82 2255 29 170 209
Fragments arranged by size:
2255 209 170 82 29
DraIII CAC_nnn'GTG
Cuts at: 0 1718 2745
Size: 1718 1027
EaeI y'GGCC_r
Cuts at: 0 1429 1749 2745
Size: 1429 320 996
Fragments arranged by size:
1429 996 320
EarI CTCTTCn'nnn_
Cuts at: 0 90 197 1033 1478 1763 1975 2164 2611
Size: 90 107 836 445 285 212 189 447
Cuts at: 2611 2745
Size: 134
Fragments arranged by size:
836 447 445 285 212 189 134 107
90
EcoICRI GAG'CTC
Cuts at: 0 305 2745
Size: 305 2440
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 158 221 291 728 1028 1460 1778 1868
Size: 158 63 70 437 300 432 318 90
Cuts at: 1868 1897 1999 2017 2138 2146 2209 2745
Size: 29 102 18 121 8 63 536
Fragments arranged by size:
536 437 432 318 300 158 121 102
90 70 63 63 29 18 8
EcoNI CCTnn'n_nnAGG
Cuts at: 0 2629 2745
Size: 2629 116
EcoO109I rG'GnC_Cy
Cuts at: 0 2729 2745
Size: 2729 16
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1253 2745
Size: 1253 1492
HaeII r_GCGC'y
Cuts at: 0 2441 2745
Size: 2441 304
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 330 362 493 525 538 570 592 624
Size: 330 32 131 32 13 32 22 32
Cuts at: 624 1074 1106 1427 1459 1764 1796 1908 1940
Size: 450 32 321 32 305 32 112 32
Cuts at: 1940 2503 2528 2535 2560 2745
Size: 563 25 7 25 185
Fragments arranged by size:
563 450 330 321 305 185 131 112
32 32 32 32 32 32 32 32
25 25 22 13 7
HincII GTy'rAC
Cuts at: 0 2319 2745
Size: 2319 426
HindIII A'AGCT_T
Cuts at: 0 766 1870 2745
Size: 766 1104 875
Fragments arranged by size:
1104 875 766
KasI G'GCGC_C
Cuts at: 0 2437 2745
Size: 2437 308
KpnI G_GTAC'C
Cuts at: 0 1934 2745
Size: 1934 811
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 25 305 491 1604 2170 2745
Size: 25 280 186 1113 566 575
Fragments arranged by size:
1113 575 566 280 186 25
MscI TGG'CCA
Cuts at: 0 1431 2745
Size: 1431 1314
MspA1I CmG'CkG
Cuts at: 0 8 124 199 1534 1690 1895 1977 2687
Size: 8 116 75 1335 156 205 82 710
Cuts at: 2687 2745
Size: 58
Fragments arranged by size:
1335 710 205 156 116 82 75 58
8
NarI GG'CG_CC
Cuts at: 0 2438 2745
Size: 2438 307
NcoI C'CATG_G
Cuts at: 0 1335 2745
Size: 1335 1410
NspI r_CATG'y
Cuts at: 0 2397 2745
Size: 2397 348
PciI A'CATG_T
Cuts at: 0 2393 2745
Size: 2393 352
PflMI CCAn_nnn'nTGG
Cuts at: 0 993 1438 2745
Size: 993 445 1307
Fragments arranged by size:
1307 993 445
PfoI T'CCnGG_A
Cuts at: 0 69 1394 2123 2507 2745
Size: 69 1325 729 384 238
Fragments arranged by size:
1325 729 384 238 69
PmlI CAC'GTG
Cuts at: 0 627 2745
Size: 627 2118
PshAI GACnn'nnGTC
Cuts at: 0 2029 2186 2745
Size: 2029 157 559
Fragments arranged by size:
2029 559 157
PsiI TTA'TAA
Cuts at: 0 1868 2745
Size: 1868 877
PspXI vC'TCGA_Gb
Cuts at: 0 1541 2745
Size: 1541 1204
PstI C_TGCA'G
Cuts at: 0 89 809 1047 2332 2501 2739 2745
Size: 89 720 238 1285 169 238 6
Fragments arranged by size:
1285 720 238 238 169 89 6
PvuII CAG'CTG
Cuts at: 0 124 199 1534 1977 2687 2745
Size: 124 75 1335 443 710 58
Fragments arranged by size:
1335 710 443 124 75 58
SacI G_AGCT'C
Cuts at: 0 307 2745
Size: 307 2438
SacII CC_GC'GG
Cuts at: 0 9 2745
Size: 9 2736
SapI GCTCTTCn'nnn_
Cuts at: 0 90 2745
Size: 90 2655
SbfI CC_TGCA'GG
Cuts at: 0 2501 2745
Size: 2501 244
SfcI C'TryA_G
Cuts at: 0 85 533 805 1043 1316 1663 2328 2497
Size: 85 448 272 238 273 347 665 169
Cuts at: 2497 2735 2745
Size: 238 10
Fragments arranged by size:
665 448 347 273 272 238 238 169
85 10
SfoI GGC'GCC
Cuts at: 0 2439 2745
Size: 2439 306
SgrAI Cr'CCGG_yG
Cuts at: 0 2590 2745
Size: 2590 155
SmaI CCC'GGG
Cuts at: 0 2464 2745
Size: 2464 281
SmlI C'TyrA_G
Cuts at: 0 1541 1899 2485 2520 2745
Size: 1541 358 586 35 225
Fragments arranged by size:
1541 586 358 225 35
StuI AGG'CCT
Cuts at: 0 1005 2745
Size: 1005 1740
StyI C'CwwG_G
Cuts at: 0 676 1034 1335 2745
Size: 676 358 301 1410
Fragments arranged by size:
1410 676 358 301
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 2041 2745
Size: 2041 704
TatI w'GTAC_w
Cuts at: 0 1199 2745
Size: 1199 1546
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 294 326 1271 1303 1433 1465 1806 1838
Size: 294 32 945 32 130 32 341 32
Cuts at: 1838 2184 2216 2621 2653 2745
Size: 346 32 405 32 92
Fragments arranged by size:
945 405 346 341 294 130 92 32
32 32 32 32 32
Tth111I GACn'n_nGTC
Cuts at: 0 1075 2745
Size: 1075 1670
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 2079 2745
Size: 2079 666
XhoI C'TCGA_G
Cuts at: 0 1541 2745
Size: 1541 1204
XmaI C'CCGG_G
Cuts at: 0 2462 2745
Size: 2462 283
XmnI GAAnn'nnTTC
Cuts at: 0 164 2745
Size: 164 2581
Enzymes that do cut:
AarI Acc65I AcuI AflII AflIII AloI AlwNI ApaLI
ApoI AvaI BanI BanII BbeI BbsI BbvCI BcgI
BglII Bme1580I BmrI BplI BpmI Bpu10I BpuEI BsaI
BsaAI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiHKAI
BsmI Bsp1286I BspEI BspMI BsrBI BsrFI BssSI BstAPI
BstEII BstYI Bsu36I BtgI BtsI DraIII EaeI EarI
EcoICRI Eco57MI EcoNI EcoO109I FalI HaeII Hin4I HincII
HindIII KasI KpnI MmeI MscI MspA1I NarI NcoI
NspI PciI PflMI PfoI PmlI PshAI PsiI PspXI
PstI PvuII SacI SacII SapI SbfI SfcI SfoI
SgrAI SmaI SmlI StuI StyI TaqII TatI TstI
Tth111I XcmI XhoI XmaI XmnI
Enzymes that do not cut:
AatII AccI AclI AfeI AgeI AhdI AleI AlfI
ApaI AscI AseI AsiSI AvrII BaeI BamHI BciVI
BclI BfrBI BglI BlpI BmgBI BmtI BsaBI BsiEI
BsiWI BsmBI BspHI BsrDI BsrGI BssHII BstBI BstXI
BstZ17I BtgZI ClaI CspCI DraI DrdI EagI EciI
EcoRI EcoRV FseI FspI FspAI HpaI MfeI MluI
MslI NaeI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PmeI PpiI PpuMI PspOMI PsrI PvuI
RsrII SalI SanDI ScaI SexAI SfiI SgfI SnaBI
SpeI SphI SrfI SspI SwaI XbaI ZraI