(Circular) (Six-base) MAPSORT of: pFN21ASDA0141.seq Check: 2964 from: 1 to: 6274 pFN21ASDA0141 6274 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4084 278 Size: 53 83 186 936 2548 2468 Fragments arranged by size: 2548 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3617 1937 Size: 1680 4594 Acc65I G'GTAC_C Cuts at: 3186 3595 3186 Size: 409 5865 AclI AA'CG_TT Cuts at: 1563 4401 4774 1563 Size: 2838 373 3063 Fragments arranged by size: 3063 2838 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3068 3386 4337 5721 5989 1632 Size: 1436 318 951 1384 268 1917 Fragments arranged by size: 1917 1436 1384 951 318 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6077 AflIII A'CryG_T Cuts at: 1203 5173 6150 1203 Size: 3970 977 1327 Fragments arranged by size: 3970 1327 977 AgeI A'CCGG_T Cuts at: 5105 5105 Size: 6274 AhdI GACnn_n'nnGTC Cuts at: 5003 5003 Size: 6274 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6274 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2883 1184 Size: 1699 4575 AlwNI CAG_nnn'CTG Cuts at: 4017 5589 4017 Size: 1572 4702 ApaI G_GGCC'C Cuts at: 3342 3342 Size: 6274 ApaLI G'TGCA_C Cuts at: 4331 5487 4331 Size: 1156 5118 ApoI r'AATT_y Cuts at: 1493 3198 3583 3809 3961 6070 1493 Size: 1705 385 226 152 2109 1697 Fragments arranged by size: 2109 1705 1697 385 226 152 AseI AT'TA_AT Cuts at: 160 1032 4828 160 Size: 872 3796 1606 Fragments arranged by size: 3796 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6274 AvaI C'yCGr_G Cuts at: 1870 1943 2035 3092 3334 3439 3599 1870 Size: 73 92 1057 242 105 160 4545 Fragments arranged by size: 4545 1057 242 160 105 92 73 BamHI G'GATC_C Cuts at: 3604 3971 3604 Size: 367 5907 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2028 2421 2897 2985 3186 Size: 325 217 627 241 393 476 88 201 Cuts at: 3186 3595 5050 618 Size: 409 1455 1842 Fragments arranged by size: 1842 1455 627 476 409 393 325 241 217 201 88 BanII G_rGCy'C Cuts at: 1391 2056 2569 3117 3310 3342 3593 1391 Size: 665 513 548 193 32 251 4072 Fragments arranged by size: 4072 665 548 513 251 193 32 BbeI G_GCGC'C Cuts at: 2032 2032 Size: 6274 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2235 928 Size: 971 336 4967 Fragments arranged by size: 4967 971 336 BbvCI CC'TCA_GC Cuts at: 2358 2524 2661 2358 Size: 166 137 5971 Fragments arranged by size: 5971 166 137 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4465 4499 1903 Size: 34 2528 34 3678 Fragments arranged by size: 3678 2528 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4166 5376 4166 Size: 1210 5064 BclI T'GATC_A Cuts at: 1548 1548 Size: 6274 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2303 2466 4885 Size: 107 122 71 631 605 631 163 2419 Cuts at: 4885 136 Size: 1525 Fragments arranged by size: 2419 1525 631 631 605 163 122 107 71 BglII A'GATC_T Cuts at: 6269 6269 Size: 6274 BlpI GC'TnA_GC Cuts at: 2529 3691 2529 Size: 1162 5112 Bme1580I G_kGCm'C Cuts at: 1790 2291 3342 4335 5491 1790 Size: 501 1051 993 1156 2573 Fragments arranged by size: 2573 1156 1051 993 501 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6274 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2078 4005 4963 Size: 416 53 83 399 24 661 1927 958 Cuts at: 4963 442 Size: 1753 Fragments arranged by size: 1927 1753 958 661 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 3413 1056 Size: 2357 3917 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2109 2583 2109 Size: 474 5800 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2104 3361 3487 4934 1239 Size: 865 1257 126 1447 2579 Fragments arranged by size: 2579 1447 1257 865 126 Bpu10I CC'TnA_GC Cuts at: 2358 2524 2661 3021 2358 Size: 166 137 360 5611 Fragments arranged by size: 5611 360 166 137 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2729 3091 3268 4399 5264 5562 2729 Size: 362 177 1131 865 298 3441 Fragments arranged by size: 3441 1131 865 362 298 177 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2991 4937 882 Size: 617 1492 1946 2219 Fragments arranged by size: 2219 1946 1492 617 BsaAI yAC'GTr Cuts at: 493 1204 3571 4065 493 Size: 711 2367 494 2702 Fragments arranged by size: 2702 2367 711 494 BsaBI GATnn'nnATC Cuts at: 6268 6268 Size: 6274 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2029 4081 4463 5098 Size: 53 83 186 936 496 2052 382 635 Cuts at: 5098 275 Size: 1451 Fragments arranged by size: 2052 1451 936 635 496 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4707 5105 5379 5526 5937 4707 Size: 398 274 147 411 5044 Fragments arranged by size: 5044 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2414 2436 2444 2466 1178 Size: 30 165 30 1011 22 8 22 4986 Fragments arranged by size: 4986 1011 165 30 30 22 22 8 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2122 2422 2577 2726 3452 1186 Size: 936 300 155 149 726 4008 Fragments arranged by size: 4008 936 726 300 155 149 BseYI C'CCAG_C Cuts at: 2456 2470 2655 3001 3341 5477 5973 2456 Size: 14 185 346 340 2136 496 2757 Fragments arranged by size: 2757 2136 496 346 340 185 14 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3245 1163 Size: 573 1509 4192 Fragments arranged by size: 4192 1509 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3724 4485 5513 1540 Size: 275 186 1723 761 1028 2301 Fragments arranged by size: 2301 1723 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2056 2569 3446 4335 4420 5491 2056 Size: 513 877 889 85 1071 2839 Fragments arranged by size: 2839 1071 889 877 513 85 BsmI GAATG_Cn' Cuts at: 3785 3878 3785 Size: 93 6181 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2056 2291 2569 3117 3310 3342 3446 Size: 399 266 235 278 548 193 32 104 Cuts at: 3446 3593 4335 4420 5491 1391 Size: 147 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 548 399 278 266 235 193 147 104 85 32 BspEI T'CCGG_A Cuts at: 5937 5937 Size: 6274 BspHI T'CATG_A Cuts at: 2881 4163 5893 2881 Size: 1282 1730 3262 Fragments arranged by size: 3262 1730 1282 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3174 3629 844 Size: 2330 455 3489 Fragments arranged by size: 3489 2330 455 BsrBI CCG'CTC Cuts at: 1394 1823 2196 3025 3272 4161 1394 Size: 429 373 829 247 889 3507 Fragments arranged by size: 3507 889 829 429 373 247 BsrDI GCAATG_nn' Cuts at: 65 1248 4769 4943 65 Size: 1183 3521 174 1396 Fragments arranged by size: 3521 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4918 5105 1700 Size: 110 3108 187 2869 Fragments arranged by size: 3108 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6274 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6274 BssSI C'ACGA_G Cuts at: 2191 4334 5346 2191 Size: 2143 1012 3119 Fragments arranged by size: 3119 2143 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6274 BstBI TT'CG_AA Cuts at: 2007 5078 2007 Size: 3071 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6274 BstXI CCAn_nnnn'nTGG Cuts at: 2143 2143 Size: 6274 BstYI r'GATC_y Cuts at: 1972 2325 3604 3971 4356 4373 5825 5911 5923 Size: 353 1279 367 385 17 1452 86 12 Cuts at: 5923 6269 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1279 385 367 353 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2492 2574 2492 Size: 82 6192 BtgI C'CryG_G Cuts at: 513 1065 2179 2212 2842 3318 513 Size: 552 1114 33 630 476 3469 Fragments arranged by size: 3469 1114 630 552 476 33 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2743 487 Size: 683 56 1517 4018 Fragments arranged by size: 4018 1517 683 56 BtsI GCAGTG_nn' Cuts at: 2371 2435 3042 3790 4584 4604 2371 Size: 64 607 748 794 20 4041 Fragments arranged by size: 4041 794 748 607 64 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6274 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6239 DraI TTT'AAA Cuts at: 3578 3578 Size: 6274 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2098 5281 809 Size: 507 782 3183 1802 Fragments arranged by size: 3183 1802 782 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2295 2509 3721 Size: 54 1425 50 275 18 465 214 1212 Cuts at: 3721 4610 6239 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1212 889 465 275 214 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3721 1537 Size: 275 1909 4090 Fragments arranged by size: 4090 1909 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2061 2744 2786 3013 4204 1353 Size: 708 683 42 227 1191 3423 Fragments arranged by size: 3423 1191 708 683 227 42 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4861 5235 5381 387 Size: 1064 138 3272 374 146 1280 Fragments arranged by size: 3272 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2054 2567 2054 Size: 513 5761 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2104 3068 3361 3386 3487 4337 4934 Size: 393 472 964 293 25 101 850 597 Cuts at: 4934 5721 5989 1239 Size: 787 268 1524 Fragments arranged by size: 1524 964 850 787 597 472 393 293 268 101 25 EcoNI CCTnn'n_nnAGG Cuts at: 2711 3505 2711 Size: 794 5480 EcoO109I rG'GnC_Cy Cuts at: 2072 2174 2208 2354 2578 2594 3338 2072 Size: 102 34 146 224 16 744 5008 Fragments arranged by size: 5008 744 224 146 102 34 16 EcoRI G'AATT_C Cuts at: 3583 3961 3583 Size: 378 5896 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2245 2245 Size: 6274 FspI TGC'GCA Cuts at: 4780 4780 Size: 6274 HaeII r_GCGC'y Cuts at: 1678 2032 5421 1678 Size: 354 3389 2531 Fragments arranged by size: 3389 2531 354 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2064 2096 2185 2217 2264 Size: 32 135 32 492 32 89 32 47 Cuts at: 2264 2296 2433 2465 2990 3022 4915 4947 4989 Size: 32 137 32 525 32 1893 32 42 Cuts at: 4989 5021 1373 Size: 32 2626 Fragments arranged by size: 2626 1893 525 492 137 135 89 47 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3618 3864 669 Size: 988 281 1680 246 3079 Fragments arranged by size: 3079 1680 988 281 246 HindIII A'AGCT_T Cuts at: 748 3084 748 Size: 2336 3938 HpaI GTT'AAC Cuts at: 3864 3864 Size: 6274 KasI G'GCGC_C Cuts at: 2028 2028 Size: 6274 KpnI G_GTAC'C Cuts at: 3190 3599 3190 Size: 409 5865 MfeI C'AATT_G Cuts at: 3873 3873 Size: 6274 MluI A'CGCG_T Cuts at: 6150 6150 Size: 6274 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5388 5572 1305 Size: 4083 184 2007 Fragments arranged by size: 4083 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2511 10 Size: 54 1425 343 679 3773 Fragments arranged by size: 3773 1425 679 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2886 4232 4591 4750 518 Size: 648 1720 1346 359 159 2042 Fragments arranged by size: 2042 1720 1346 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2314 2362 2844 3690 4367 5515 5760 1606 Size: 708 48 482 846 677 1148 245 2120 Fragments arranged by size: 2120 1148 846 708 677 482 245 48 NarI GG'CG_CC Cuts at: 2029 2029 Size: 6274 NcoI C'CATG_G Cuts at: 513 1065 2179 2212 3318 513 Size: 552 1114 33 1106 3469 Fragments arranged by size: 3469 1114 1106 552 33 NdeI CA'TA_TG Cuts at: 387 387 Size: 6274 NheI G'CTAG_C Cuts at: 1052 3409 1052 Size: 2357 3917 NotI GC'GGCC_GC Cuts at: 3721 3721 Size: 6274 NspI r_CATG'y Cuts at: 1132 1229 2621 3437 3632 5177 1132 Size: 97 1392 816 195 1545 2229 Fragments arranged by size: 2229 1545 1392 816 195 97 PasI CC'CwG_GG Cuts at: 1348 2904 1348 Size: 1556 4718 PciI A'CATG_T Cuts at: 5173 5173 Size: 6274 PflMI CCAn_nnn'nTGG Cuts at: 1108 2283 2962 1108 Size: 1175 679 4420 Fragments arranged by size: 4420 1175 679 PmeI GTTT'AAAC Cuts at: 3578 3578 Size: 6274 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4343 4375 5856 5888 4343 Size: 32 1481 32 4729 Fragments arranged by size: 4729 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2072 2174 2208 2354 2578 2594 2072 Size: 102 34 146 224 16 5752 Fragments arranged by size: 5752 224 146 102 34 16 PshAI GACnn'nnGTC Cuts at: 1613 3367 1613 Size: 1754 4520 PsiI TTA'TAA Cuts at: 3844 3844 Size: 6274 PspOMI G'GGCC_C Cuts at: 3338 3338 Size: 6274 PspXI vC'TCGA_Gb Cuts at: 3439 3439 Size: 6274 PstI C_TGCA'G Cuts at: 830 2313 2839 3061 3331 3395 3470 3626 830 Size: 1483 526 222 270 64 75 156 3478 Fragments arranged by size: 3478 1483 526 270 222 156 75 64 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6274 PvuII CAG'CTG Cuts at: 2314 2314 Size: 6274 SacI G_AGCT'C Cuts at: 2056 2569 2056 Size: 513 5761 SacII CC_GC'GG Cuts at: 2845 2845 Size: 6274 SalI G'TCGA_C Cuts at: 1936 3616 1936 Size: 1680 4594 SapI GCTCTTCn'nnn_ Cuts at: 2061 2744 2061 Size: 683 5591 SbfI CC_TGCA'GG Cuts at: 3626 3626 Size: 6274 ScaI AGT'ACT Cuts at: 4522 6234 4522 Size: 1712 4562 SfcI C'TryA_G Cuts at: 826 1045 2309 2835 2848 3057 3327 3391 3466 Size: 219 1264 526 13 209 270 64 75 Cuts at: 3466 3622 4757 5438 5629 826 Size: 156 1135 681 191 1471 Fragments arranged by size: 1471 1264 1135 681 526 270 219 209 191 156 75 64 13 SfiI GGCCn_nnn'nGGCC Cuts at: 2303 2303 Size: 6274 SfoI GGC'GCC Cuts at: 2030 2030 Size: 6274 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6274 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6274 SmaI CCC'GGG Cuts at: 1872 2037 3336 3601 1872 Size: 165 1299 265 4545 Fragments arranged by size: 4545 1299 265 165 SmlI C'TyrA_G Cuts at: 820 1017 1943 2708 3070 3247 3439 4378 5279 Size: 197 926 765 362 177 192 939 901 Cuts at: 5279 5541 820 Size: 262 1553 Fragments arranged by size: 1553 939 926 901 765 362 262 197 192 177 SnaBI TAC'GTA Cuts at: 493 3571 4065 493 Size: 3078 494 2702 Fragments arranged by size: 3078 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6274 SphI G_CATG'C Cuts at: 1132 2621 3632 1132 Size: 1489 1011 3774 Fragments arranged by size: 3774 1489 1011 SspI AAT'ATT Cuts at: 5 52 4198 5 Size: 47 4146 2081 Fragments arranged by size: 4146 2081 47 StuI AGG'CCT Cuts at: 1517 3216 3245 1517 Size: 1699 29 4546 Fragments arranged by size: 4546 1699 29 StyI C'CwwG_G Cuts at: 513 1065 2179 2204 2212 3108 3193 3217 3318 Size: 552 1114 25 8 896 85 24 101 Cuts at: 3318 3454 3713 513 Size: 136 259 3074 Fragments arranged by size: 3074 1114 896 552 259 136 101 85 25 24 8 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2439 4301 4318 4471 4656 5101 325 Size: 1255 859 1862 17 153 185 445 1498 Fragments arranged by size: 1862 1498 1255 859 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2862 4520 6232 Size: 274 80 33 51 1443 885 1658 1712 Cuts at: 6232 96 Size: 138 Fragments arranged by size: 1712 1658 1443 885 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2414 2446 6234 6266 2414 Size: 32 3788 32 2422 Fragments arranged by size: 3788 2422 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6274 XbaI T'CTAG_A Cuts at: 3610 3610 Size: 6274 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3009 3009 Size: 6274 XhoI C'TCGA_G Cuts at: 1943 3439 1943 Size: 1496 4778 XmaI C'CCGG_G Cuts at: 1870 2035 3334 3599 1870 Size: 165 1299 265 4545 Fragments arranged by size: 4545 1299 265 165 XmnI GAAnn'nnTTC Cuts at: 3371 4403 3371 Size: 1032 5242 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4082 276 Size: 53 83 186 936 2548 2468 Fragments arranged by size: 2548 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoRV FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PmlI PsrI RsrII SanDI SexAI SrfI SwaI