(Circular) (Six-base) MAPSORT of: pFN21ASDA0140.seq Check: 9437 from: 1 to: 5995 pFN21ASDA0140 5995 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2633 2779 2633 Size: 146 5849 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3805 278 Size: 53 83 186 936 2269 2468 Fragments arranged by size: 2468 2269 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3338 1937 Size: 1401 4594 Acc65I G'GTAC_C Cuts at: 3316 3316 Size: 5995 AclI AA'CG_TT Cuts at: 1563 4122 4495 1563 Size: 2559 373 3063 Fragments arranged by size: 3063 2559 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2071 2180 2482 2509 2898 2926 4058 5442 Size: 439 109 302 27 389 28 1132 1384 Cuts at: 5442 5710 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 1132 439 389 302 268 109 28 27 AfeI AGC'GCT Cuts at: 2483 2483 Size: 5995 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5798 AflIII A'CryG_T Cuts at: 1203 4894 5871 1203 Size: 3691 977 1327 Fragments arranged by size: 3691 1327 977 AgeI A'CCGG_T Cuts at: 4826 4826 Size: 5995 AhdI GACnn_n'nnGTC Cuts at: 4724 4724 Size: 5995 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5995 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5995 AlwNI CAG_nnn'CTG Cuts at: 2223 3027 3243 3738 5310 2223 Size: 804 216 495 1572 2908 Fragments arranged by size: 2908 1572 804 495 216 ApaI G_GGCC'C Cuts at: 3229 3229 Size: 5995 ApaLI G'TGCA_C Cuts at: 4052 5208 4052 Size: 1156 4839 ApoI r'AATT_y Cuts at: 1493 3304 3530 3682 5791 1493 Size: 1811 226 152 2109 1697 Fragments arranged by size: 2109 1811 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4549 160 Size: 872 3517 1606 Fragments arranged by size: 3517 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5995 AvaI C'yCGr_G Cuts at: 1870 1943 2055 2538 3320 1870 Size: 73 112 483 782 4545 Fragments arranged by size: 4545 782 483 112 73 BamHI G'GATC_C Cuts at: 3325 3692 3325 Size: 367 5628 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2259 2614 2871 3316 4771 Size: 325 217 627 472 355 257 445 1455 Cuts at: 4771 618 Size: 1842 Fragments arranged by size: 1842 1455 627 472 445 355 325 257 217 BanII G_rGCy'C Cuts at: 1391 2426 2660 2996 3229 3314 1391 Size: 1035 234 336 233 85 4072 Fragments arranged by size: 4072 1035 336 234 233 85 BbeI G_GCGC'C Cuts at: 2875 2875 Size: 5995 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5024 BbvCI CC'TCA_GC Cuts at: 2048 2309 2338 2755 2951 2048 Size: 261 29 417 196 5092 Fragments arranged by size: 5092 417 261 196 29 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4186 4220 1903 Size: 34 2249 34 3678 Fragments arranged by size: 3678 2249 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3887 5097 3887 Size: 1210 4785 BclI T'GATC_A Cuts at: 1548 2298 1548 Size: 750 5245 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2921 3060 4606 Size: 107 122 71 631 605 1249 139 1546 Cuts at: 4606 136 Size: 1525 Fragments arranged by size: 1546 1525 1249 631 605 139 122 107 71 BglII A'GATC_T Cuts at: 5990 5990 Size: 5995 BlpI GC'TnA_GC Cuts at: 2623 3412 2623 Size: 789 5206 Bme1580I G_kGCm'C Cuts at: 1790 3229 4056 5212 1790 Size: 1439 827 1156 2573 Fragments arranged by size: 2573 1439 1156 827 BmgBI CAC'GTC Cuts at: 1318 3010 1318 Size: 1692 4303 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3726 4684 442 Size: 416 53 83 399 24 2309 958 1753 Fragments arranged by size: 2309 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5995 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2919 2919 Size: 5995 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2674 2785 4655 1239 Size: 1435 111 1870 2579 Fragments arranged by size: 2579 1870 1435 111 Bpu10I CC'TnA_GC Cuts at: 2048 2309 2338 2755 2951 2960 3090 2048 Size: 261 29 417 196 9 130 4953 Fragments arranged by size: 4953 417 261 196 130 29 9 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4120 4985 5283 4120 Size: 865 298 4832 Fragments arranged by size: 4832 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2166 4658 882 Size: 617 667 2492 2219 Fragments arranged by size: 2492 2219 667 617 BsaAI yAC'GTr Cuts at: 493 1204 3292 3786 493 Size: 711 2088 494 2702 Fragments arranged by size: 2702 2088 711 494 BsaBI GATnn'nnATC Cuts at: 5989 5989 Size: 5995 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2872 3802 4184 4819 Size: 53 83 186 936 1339 930 382 635 Cuts at: 4819 275 Size: 1451 Fragments arranged by size: 1451 1339 936 930 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4428 4826 5100 5247 5658 4428 Size: 398 274 147 411 4765 Fragments arranged by size: 4765 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2537 2567 3125 3155 1178 Size: 30 165 30 1134 30 558 30 4018 Fragments arranged by size: 4018 1134 558 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2527 2545 2694 3164 1186 Size: 1341 18 149 470 4017 Fragments arranged by size: 4017 1341 470 149 18 BseYI C'CCAG_C Cuts at: 2826 3074 5198 5694 2826 Size: 248 2124 496 3127 Fragments arranged by size: 3127 2124 496 248 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2084 2651 1163 Size: 573 348 567 4507 Fragments arranged by size: 4507 573 567 348 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3445 4206 5234 1540 Size: 275 186 1444 761 1028 2301 Fragments arranged by size: 2301 1444 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2216 2553 4056 4141 5212 2216 Size: 337 1503 85 1071 2999 Fragments arranged by size: 2999 1503 1071 337 85 BsmI GAATG_Cn' Cuts at: 2234 3506 3599 2234 Size: 1272 93 4630 Fragments arranged by size: 4630 1272 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2446 2788 2446 Size: 342 5653 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2216 2426 2553 2660 2996 3229 3314 Size: 399 426 210 127 107 336 233 85 Cuts at: 3314 4056 4141 5212 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 426 399 336 233 210 127 107 85 85 BspEI T'CCGG_A Cuts at: 5658 5658 Size: 5995 BspHI T'CATG_A Cuts at: 3884 5614 3884 Size: 1730 4265 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2633 2673 2703 2779 3350 844 Size: 1789 40 30 76 571 3489 Fragments arranged by size: 3489 1789 571 76 40 30 BsrBI CCG'CTC Cuts at: 1394 1823 2367 2823 3194 3882 1394 Size: 429 544 456 371 688 3507 Fragments arranged by size: 3507 688 544 456 429 371 BsrDI GCAATG_nn' Cuts at: 65 1248 2070 4490 4664 65 Size: 1183 822 2420 174 1396 Fragments arranged by size: 2420 1396 1183 822 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2685 3199 4639 4826 1700 Size: 110 875 514 1440 187 2869 Fragments arranged by size: 2869 1440 875 514 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5995 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5995 BssSI C'ACGA_G Cuts at: 4055 5067 4055 Size: 1012 4983 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5995 BstBI TT'CG_AA Cuts at: 2007 4799 2007 Size: 2792 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5995 BstXI CCAn_nnnn'nTGG Cuts at: 2029 2591 2029 Size: 562 5433 BstYI r'GATC_y Cuts at: 1972 3325 3692 4077 4094 5546 5632 5644 5990 Size: 1353 367 385 17 1452 86 12 346 Cuts at: 5990 1972 Size: 1977 Fragments arranged by size: 1977 1452 1353 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2442 2673 3220 513 Size: 552 957 420 231 547 3288 Fragments arranged by size: 3288 957 552 547 420 231 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5256 Fragments arranged by size: 5256 683 56 BtsI GCAGTG_nn' Cuts at: 2277 3511 4305 4325 2277 Size: 1234 794 20 3947 Fragments arranged by size: 3947 1234 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5995 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5960 DraI TTT'AAA Cuts at: 3299 3299 Size: 5995 DraIII CAC_nnn'GTG Cuts at: 2674 2674 Size: 5995 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2852 3122 5002 809 Size: 507 1536 270 1880 1802 Fragments arranged by size: 1880 1802 1536 507 270 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3442 4331 5960 Size: 54 1425 50 275 18 1612 889 1629 Cuts at: 5960 8 Size: 43 Fragments arranged by size: 1629 1612 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3442 1537 Size: 275 1630 4090 Fragments arranged by size: 4090 1630 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2199 3102 3925 1353 Size: 846 903 823 3423 Fragments arranged by size: 3423 903 846 823 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4582 4956 5102 387 Size: 1064 138 2993 374 146 1280 Fragments arranged by size: 2993 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2071 2180 2482 2509 2674 2785 2898 Size: 393 439 109 302 27 165 111 113 Cuts at: 2898 2926 4058 4655 5442 5710 1239 Size: 28 1132 597 787 268 1524 Fragments arranged by size: 1524 1132 787 597 439 393 302 268 165 113 111 109 28 27 EcoNI CCTnn'n_nnAGG Cuts at: 3173 3173 Size: 5995 EcoO109I rG'GnC_Cy Cuts at: 2082 2126 2415 3024 3066 3078 3136 3225 3226 Size: 44 289 609 42 12 58 89 1 Cuts at: 3226 2082 Size: 4851 Fragments arranged by size: 4851 609 289 89 58 44 42 12 1 EcoRI G'AATT_C Cuts at: 3304 3682 3304 Size: 378 5617 FspI TGC'GCA Cuts at: 4501 4501 Size: 5995 HaeII r_GCGC'y Cuts at: 1678 2359 2485 2875 5142 1678 Size: 681 126 390 2267 2531 Fragments arranged by size: 2531 2267 681 390 126 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2375 2407 3125 3157 4636 Size: 32 135 32 803 32 718 32 1479 Cuts at: 4636 4668 4710 4742 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1479 803 718 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3339 3585 669 Size: 988 281 1401 246 3079 Fragments arranged by size: 3079 1401 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5995 HpaI GTT'AAC Cuts at: 3585 3585 Size: 5995 KasI G'GCGC_C Cuts at: 2871 2871 Size: 5995 KpnI G_GTAC'C Cuts at: 3320 3320 Size: 5995 MfeI C'AATT_G Cuts at: 3594 3594 Size: 5995 MluI A'CGCG_T Cuts at: 5871 5871 Size: 5995 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2514 3243 5109 5293 1305 Size: 1209 729 1866 184 2007 Fragments arranged by size: 2007 1866 1209 729 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4173 Fragments arranged by size: 4173 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2027 3953 4312 4471 518 Size: 648 861 1926 359 159 2042 Fragments arranged by size: 2042 1926 861 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2466 2701 2805 2955 3074 3162 3222 3411 Size: 860 235 104 150 119 88 60 189 Cuts at: 3411 4088 5236 5481 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 860 677 245 235 189 150 119 104 88 60 NaeI GCC'GGC Cuts at: 2687 3201 2687 Size: 514 5481 NarI GG'CG_CC Cuts at: 2872 2872 Size: 5995 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4486 Fragments arranged by size: 4486 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5995 NgoMIV G'CCGG_C Cuts at: 2685 3199 2685 Size: 514 5481 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5995 NotI GC'GGCC_GC Cuts at: 3442 3442 Size: 5995 NspI r_CATG'y Cuts at: 1132 1229 2079 3353 4898 1132 Size: 97 850 1274 1545 2229 Fragments arranged by size: 2229 1545 1274 850 97 PasI CC'CwG_GG Cuts at: 1348 3056 3230 1348 Size: 1708 174 4113 Fragments arranged by size: 4113 1708 174 PciI A'CATG_T Cuts at: 4894 4894 Size: 5995 PflMI CCAn_nnn'nTGG Cuts at: 1108 2223 2433 3027 1108 Size: 1115 210 594 4076 Fragments arranged by size: 4076 1115 594 210 PmeI GTTT'AAAC Cuts at: 3299 3299 Size: 5995 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4064 4096 5577 5609 4064 Size: 32 1481 32 4450 Fragments arranged by size: 4450 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2082 2126 3066 3078 3136 2082 Size: 44 940 12 58 4941 Fragments arranged by size: 4941 940 58 44 12 PshAI GACnn'nnGTC Cuts at: 1613 2982 3083 1613 Size: 1369 101 4525 Fragments arranged by size: 4525 1369 101 PsiI TTA'TAA Cuts at: 3565 3565 Size: 5995 PspOMI G'GGCC_C Cuts at: 3225 3225 Size: 5995 PstI C_TGCA'G Cuts at: 830 2700 3208 3347 830 Size: 1870 508 139 3478 Fragments arranged by size: 3478 1870 508 139 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5995 PvuII CAG'CTG Cuts at: 2955 3162 2955 Size: 207 5788 SacII CC_GC'GG Cuts at: 3223 3223 Size: 5995 SalI G'TCGA_C Cuts at: 1936 3337 1936 Size: 1401 4594 SanDI GG'GwC_CC Cuts at: 3078 3078 Size: 5995 SbfI CC_TGCA'GG Cuts at: 3347 3347 Size: 5995 ScaI AGT'ACT Cuts at: 4243 5955 4243 Size: 1712 4283 SfcI C'TryA_G Cuts at: 826 1045 2461 2696 3204 3343 4478 5159 5350 Size: 219 1416 235 508 139 1135 681 191 Cuts at: 5350 826 Size: 1471 Fragments arranged by size: 1471 1416 1135 681 508 235 219 191 139 SfoI GGC'GCC Cuts at: 2873 2873 Size: 5995 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5995 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5995 SmaI CCC'GGG Cuts at: 1872 2057 2540 3322 1872 Size: 185 483 782 4545 Fragments arranged by size: 4545 782 483 185 SmlI C'TyrA_G Cuts at: 820 1017 1943 4099 5000 5262 820 Size: 197 926 2156 901 262 1553 Fragments arranged by size: 2156 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3292 3786 493 Size: 2799 494 2702 Fragments arranged by size: 2799 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5995 SphI G_CATG'C Cuts at: 1132 3353 1132 Size: 2221 3774 SspI AAT'ATT Cuts at: 5 52 3919 5 Size: 47 3867 2081 Fragments arranged by size: 3867 2081 47 StuI AGG'CCT Cuts at: 1517 2899 1517 Size: 1382 4613 StyI C'CwwG_G Cuts at: 513 1065 2022 2418 2608 3434 513 Size: 552 957 396 190 826 3074 Fragments arranged by size: 3074 957 826 552 396 190 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4022 4039 4192 4377 4822 325 Size: 1255 2442 17 153 185 445 1498 Fragments arranged by size: 2442 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4241 5953 96 Size: 274 80 33 51 1443 2264 1712 138 Fragments arranged by size: 2264 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5955 5987 5955 Size: 32 5963 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5995 XbaI T'CTAG_A Cuts at: 2905 3331 2905 Size: 426 5569 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2588 2588 Size: 5995 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5995 XmaI C'CCGG_G Cuts at: 1870 2055 2538 3320 1870 Size: 185 483 782 4545 Fragments arranged by size: 4545 782 483 185 XmnI GAAnn'nnTTC Cuts at: 4124 4124 Size: 5995 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3803 276 Size: 53 83 186 936 2269 2468 Fragments arranged by size: 2468 2269 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BfrBI BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspXI PsrI RsrII SacI SapI SexAI SfiI SrfI SwaI