Restriction map for
pFN21ASDA0137
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# NO CUT ENZYMES ( 122 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AcyI AflIII AhdI AocI Aor51HI
ApaI AscI AseI AsnI Asp700I
Asp718I AspEI AspI AviII AvrII
BbeI BbrPI BbsI BbvCI BcgI
BclI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaHI BsaI BseAI
BsgI BsiWI BsmI BspEI BspLU11I
BspMI BsrBI BssHII BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstUI
BstZ17I Bsu36I CelII CpoI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO65I
EcoRI EcoT22I FauI FbaI FseI
FspI Hin1I HpaI KasI KpnI
KspI MfeI MluI MroI MunI
MvnI NaeI NarI NcoI NdeI
NgoMIV NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PmaCI
PmeI PmlI PshAI PshBI PvuI
RsrII SacI SacII SalI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SnaBI SpeI SphI
Sse8387I StuI SwaI Tth111I XbaI
XhoI XmnI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AclI 1265 AACGTT AA^CGTT
AflII 1759 CTTAAG C^TTAAG
AgeI 2100 ACCGGT A^CCGGT
Alw44I 2173 GTGCAC G^TGCAC
AlwNI 688 CAGGACCTG CAG[ACGT][ACGT][ACGT]^CTG
ApaLI 2173 GTGCAC G^TGCAC
BalI 426 TGGCCA TGG^CCA
BamHI 165 GGATCC G^GATCC
BanI 1074 GGCACC G^G[CT][GA]CC
BanII 75 GGGCTC G[GA]GC[CT]^C
BciVI 1587 GGATAC GTATCC(6/5)
BfrI 1759 CTTAAG C^TTAAG
BsaAI 1295 TACGTG [CT]AC^GT[GA]
BsaBI 1639 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BseRI 509 CTCCTC GAGGAG(10/8)
BsiEI 553 CGACCG CG[GA][CT]^CG
BsmBI 62 CGTCTC CGTCTC(1/5)
BspHI 1188 TCATGA T^CATGA
BsrFI 2100 ACCGGT [GA]^CCGG[CT]
BstXI 1832 CCAAGATTATGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 926 GCGC GCG^C
Cfr10I 2100 ACCGGT [GA]^CCGG[CT]
DraI 625 TTTAAA TTT^AAA
EcoRV 1718 GATATC GAT^ATC
HaeII 925 GGCGCT [GA]GCGC^[CT]
HgaI 2135 GACGC GACGC(5/10)
HhaI 926 GCGC GCG^C
HinP1I 926 GCGC G^CGC
HincII 1170 GTTGAC GT[CT]^[GA]AC
HindII 1170 GTTGAC GT[CT]^[GA]AC
HindIII 297 AAGCTT A^AGCTT
MamI 1639 GATTTCTATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 426 TGGCCA TGG^CCA
MscI 426 TGGCCA TGG^CCA
MspA1I 2179 CAGCTG C[AC]G^C[GT]G
MwoI 82 GCGGCGGCGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PflMI 687 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PinAI 2100 ACCGGT A^CCGGT
Psp1406I 1265 AACGTT AA^CGTT
PstI 1515 CTGCAG CTGCA^G
PvuII 2179 CAGCTG CAG^CTG
RcaI 1188 TCATGA T^CATGA
SfaNI 2260 GCATC GCATC(5/9)
SfcI 1515 CTGCAG C^T[GA][CT]AG
SmaI 72 CCCGGG CCC^GGG
SspI 2064 AATATT AAT^ATT
Van91I 687 CCAGGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 72 CCCGGG C^CCGGG
XmaI 72 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 29 enzymes) #
###################################
AspHI 219 GAGCAC G[AT]GC[AT]^C
2173 GTGCAC
AvaI 72 CCCGGG C^[CT]CG[GA]G
898 CTCGGG
BbvI 664 GCAGC GCAGC(8/12)
1444 GCTGC
BpmI 203 CTGGAG CTGGAG(16/14)
1476 CTGGAG
BsaWI 333 ACCGGA [AT]^CCGG[AT]
2100 ACCGGT
BsiHKAI 219 GAGCAC G[AT]GC[AT]^C
2173 GTGCAC
BsmAI 328 GAGAC GTCTC(1/5)
63 GTCTC
BsoBI 72 CCCGGG C^[CT]CG[GA]G
898 CTCGGG
Bsp1407I 603 TGTACA T^GTACA
1603 TGTACA
BspDI 887 ATCGAT AT^CGAT
2162 ATCGAT
BsrDI 142 GCAATG GCAATG(2/0)
1628 GCAATG
BsrGI 603 TGTACA T^GTACA
1603 TGTACA
ClaI 887 ATCGAT AT^CGAT
2162 ATCGAT
DraII 94 AGGTCCC [GA]G^G[ACGT]CC[CT]
689 AGGACCT
EaeI 89 CGGCCA [CT]^GGCC[GA]
426 TGGCCA
EarI 873 GAAGAG CTCTTC(1/4)
1237 GAAGAG
EcoO109I 94 AGGTCCC [GA]G^G[ACGT]CC[CT]
689 AGGACCT
EcoT14I 1583 CCTTGG C^C[AT][AT]GG
2120 CCAAGG
Ksp632I 873 GAAGAG CTCTTC(1/4)
1237 GAAGAG
MaeIII 314 GTGAC ^GT[ACGT]AC
2024 GTCAC
NspI 1509 ACATGC [GA]CATG^[CT]
1795 GCATGT
PleI 1277 GAGTC GAGTC(4/5)
1982 GAGTC
PpuMI 94 AGGTCCC [GA]G^G[AT]CC[CT]
689 AGGACCT
SmlI 471 CTCAAG C^T[CT][GA]AG
1759 CTTAAG
SspBI 603 TGTACA T^GTACA
1603 TGTACA
StyI 1583 CCTTGG C^C[AT][AT]GG
2120 CCAAGG
TseI 664 GCAGC G^C[AT]GC
1444 GCTGC
Tsp45I 314 GTGAC ^GT[GC]AC
2024 GTCAC
XcmI 9 CCAAAGTAGCAGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
456 CCAGGGTGGAAATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 58 enzymes) #
#########################################
AciI AcsI AfaI AluI AlwI
ApoI AvaII BcnI BfaI BmyI
BsaJI BsiYI BslI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EcoRII
Fnu4HI FokI HaeIII HapII HinfI
HpaII HphI ItaI MaeI MaeII
MboI MboII MflI MnlI MseI
MslI MspI MvaI NciI NdeII
NlaIII NlaIV RsaI Sau3AI Sau96I
ScrFI TaqI TfiI Tru9I Tsp509I
TspRI TthHB8I XhoII