(Circular) (Six-base) MAPSORT of: pFN21ASDA0137.seq Check: 5116 from: 1 to: 7027 pFN21ASDA0137 7027 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4837 278 Size: 53 83 186 936 3301 2468 Fragments arranged by size: 3301 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4370 1937 Size: 2433 4594 Acc65I G'GTAC_C Cuts at: 4348 4348 Size: 7027 AclI AA'CG_TT Cuts at: 1563 3289 5154 5527 1563 Size: 1726 1865 373 3063 Fragments arranged by size: 3063 1865 1726 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3692 4236 5090 6474 6742 1632 Size: 2060 544 854 1384 268 1917 Fragments arranged by size: 2060 1917 1384 854 544 268 AflII C'TTAA_G Cuts at: 820 1017 3782 820 Size: 197 2765 4065 Fragments arranged by size: 4065 2765 197 AflIII A'CryG_T Cuts at: 1203 5926 6903 1203 Size: 4723 977 1327 Fragments arranged by size: 4723 1327 977 AgeI A'CCGG_T Cuts at: 4123 5858 4123 Size: 1735 5292 AhdI GACnn_n'nnGTC Cuts at: 5756 5756 Size: 7027 AleI CACnn'nnGTG Cuts at: 1166 3318 1166 Size: 2152 4875 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7027 AlwNI CAG_nnn'CTG Cuts at: 2716 4770 6342 2716 Size: 2054 1572 3401 Fragments arranged by size: 3401 2054 1572 ApaLI G'TGCA_C Cuts at: 4196 5084 6240 4196 Size: 888 1156 4983 Fragments arranged by size: 4983 1156 888 ApoI r'AATT_y Cuts at: 1493 2539 3178 3225 4246 4336 4562 4714 6823 Size: 1046 639 47 1021 90 226 152 2109 Cuts at: 6823 1493 Size: 1697 Fragments arranged by size: 2109 1697 1046 1021 639 226 152 90 47 AseI AT'TA_AT Cuts at: 160 1032 5581 160 Size: 872 4549 1606 Fragments arranged by size: 4549 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7027 AvaI C'yCGr_G Cuts at: 1870 1943 2095 2921 4352 1870 Size: 73 152 826 1431 4545 Fragments arranged by size: 4545 1431 826 152 73 BamHI G'GATC_C Cuts at: 2188 4357 4724 2188 Size: 2169 367 4491 Fragments arranged by size: 4491 2169 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3097 4348 5803 618 Size: 325 217 627 1310 1251 1455 1842 Fragments arranged by size: 1842 1455 1310 1251 627 325 217 BanII G_rGCy'C Cuts at: 1391 2102 4346 1391 Size: 711 2244 4072 Fragments arranged by size: 4072 2244 711 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6056 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5218 5252 1903 Size: 34 3281 34 3678 Fragments arranged by size: 3678 3281 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3604 4919 6129 3604 Size: 1315 1210 4502 Fragments arranged by size: 4502 1315 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 7027 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5638 136 Size: 107 122 71 631 605 3966 1525 Fragments arranged by size: 3966 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7022 7022 Size: 7027 BlpI GC'TnA_GC Cuts at: 4444 4444 Size: 7027 Bme1580I G_kGCm'C Cuts at: 1790 2928 4200 5088 6244 1790 Size: 1138 1272 888 1156 2573 Fragments arranged by size: 2573 1272 1156 1138 888 BmgBI CAC'GTC Cuts at: 1318 2085 2447 1318 Size: 767 362 5898 Fragments arranged by size: 5898 767 362 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4758 5716 442 Size: 416 53 83 399 24 3341 958 1753 Fragments arranged by size: 3341 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7027 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2247 3520 5687 1239 Size: 1008 1273 2167 2579 Fragments arranged by size: 2579 2167 1273 1008 Bpu10I CC'TnA_GC Cuts at: 2750 2750 Size: 7027 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2479 5152 6017 6315 2479 Size: 2673 865 298 3191 Fragments arranged by size: 3191 2673 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5690 882 Size: 617 4191 2219 Fragments arranged by size: 4191 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3320 4324 4818 493 Size: 711 2116 1004 494 2702 Fragments arranged by size: 2702 2116 1004 711 494 BsaBI GATnn'nnATC Cuts at: 3666 7021 3666 Size: 3355 3672 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4834 5216 5851 275 Size: 53 83 186 936 3301 382 635 1451 Fragments arranged by size: 3301 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2356 4123 5460 5858 6132 6279 6690 2356 Size: 1767 1337 398 274 147 411 2693 Fragments arranged by size: 2693 1767 1337 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6802 Fragments arranged by size: 6802 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2523 1186 Size: 1337 5690 BseYI C'CCAG_C Cuts at: 2073 4271 6230 6726 2073 Size: 2198 1959 496 2374 Fragments arranged by size: 2374 2198 1959 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6454 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2579 4477 5238 6266 1540 Size: 275 186 578 1898 761 1028 2301 Fragments arranged by size: 2301 1898 1028 761 578 275 186 BsiHKAI G_wGCw'C Cuts at: 2246 4200 5088 5173 6244 2246 Size: 1954 888 85 1071 3029 Fragments arranged by size: 3029 1954 1071 888 85 BsmI GAATG_Cn' Cuts at: 4538 4631 4538 Size: 93 6934 BsmBI CGTCTCn'nnnn_ Cuts at: 2091 2091 Size: 7027 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2102 2246 2928 4200 4346 5088 5173 Size: 399 312 144 682 1272 146 742 85 Cuts at: 5173 6244 1391 Size: 1071 2174 Fragments arranged by size: 2174 1272 1071 742 682 399 312 146 144 85 BspEI T'CCGG_A Cuts at: 6690 6690 Size: 7027 BspHI T'CATG_A Cuts at: 3211 4916 6646 3211 Size: 1705 1730 3592 Fragments arranged by size: 3592 1730 1705 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 4382 844 Size: 3538 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4914 1394 Size: 429 3091 3507 Fragments arranged by size: 3507 3091 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2172 3658 5522 5696 65 Size: 1183 924 1486 1864 174 1396 Fragments arranged by size: 1864 1486 1396 1183 924 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4123 5671 5858 1700 Size: 110 2313 1548 187 2869 Fragments arranged by size: 2869 2313 1548 187 110 BsrGI T'GTAC_A Cuts at: 96 2626 3626 96 Size: 2530 1000 3497 Fragments arranged by size: 3497 2530 1000 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7027 BssSI C'ACGA_G Cuts at: 5087 6099 5087 Size: 1012 6015 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 7027 BstBI TT'CG_AA Cuts at: 2007 5831 2007 Size: 3824 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 7027 BstXI CCAn_nnnn'nTGG Cuts at: 3862 3862 Size: 7027 BstYI r'GATC_y Cuts at: 1972 2188 2327 3895 4357 4724 5109 5126 6578 Size: 216 139 1568 462 367 385 17 1452 Cuts at: 6578 6664 6676 7022 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1568 1452 462 385 367 346 216 139 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 6475 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6288 Fragments arranged by size: 6288 683 56 BtsI GCAGTG_nn' Cuts at: 2047 2272 4141 4543 5337 5357 2047 Size: 225 1869 402 794 20 3717 Fragments arranged by size: 3717 1869 794 402 225 20 ClaI AT'CG_AT Cuts at: 1554 2911 4186 1554 Size: 1357 1275 4395 Fragments arranged by size: 4395 1357 1275 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3174 3209 529 Size: 35 2610 35 4347 Fragments arranged by size: 4347 2610 35 35 DraI TTT'AAA Cuts at: 2650 4331 2650 Size: 1681 5346 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6034 809 Size: 507 4718 1802 Fragments arranged by size: 4718 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2112 2449 4474 Size: 54 1425 50 275 18 282 337 2025 Cuts at: 4474 5363 6992 8 Size: 889 1629 43 Fragments arranged by size: 2025 1629 1425 889 337 282 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4474 1537 Size: 275 2662 4090 Fragments arranged by size: 4090 2662 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2891 3255 4957 1353 Size: 1538 364 1702 3423 Fragments arranged by size: 3423 1702 1538 364 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5614 5988 6134 387 Size: 1064 138 4025 374 146 1280 Fragments arranged by size: 4025 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2247 3520 3692 4236 5090 5687 6474 Size: 393 615 1273 172 544 854 597 787 Cuts at: 6474 6742 1239 Size: 268 1524 Fragments arranged by size: 1524 1273 854 787 615 597 544 393 268 172 EcoO109I rG'GnC_Cy Cuts at: 2118 2713 2118 Size: 595 6432 EcoRI G'AATT_C Cuts at: 4336 4714 4336 Size: 378 6649 EcoRV GAT'ATC Cuts at: 3743 3743 Size: 7027 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 4088 4088 Size: 7027 FspI TGC'GCA Cuts at: 5533 5533 Size: 7027 HaeII r_GCGC'y Cuts at: 1678 2952 6174 1678 Size: 1274 3222 2531 Fragments arranged by size: 3222 2531 1274 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2330 2362 2904 2936 3587 Size: 32 135 32 758 32 542 32 651 Cuts at: 3587 3619 3980 4012 5668 5700 5742 5774 1373 Size: 32 361 32 1656 32 42 32 2626 Fragments arranged by size: 2626 1656 758 651 542 361 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3195 4371 4617 669 Size: 988 281 1257 1176 246 3079 Fragments arranged by size: 3079 1257 1176 988 281 246 HindIII A'AGCT_T Cuts at: 748 2320 748 Size: 1572 5455 HpaI GTT'AAC Cuts at: 4617 4617 Size: 7027 KpnI G_GTAC'C Cuts at: 4352 4352 Size: 7027 MfeI C'AATT_G Cuts at: 4626 4626 Size: 7027 MluI A'CGCG_T Cuts at: 6903 6903 Size: 7027 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2114 2251 3934 3982 4002 6141 6325 1305 Size: 809 137 1683 48 20 2139 184 2007 Fragments arranged by size: 2139 2007 1683 809 184 137 48 20 MscI TGG'CCA Cuts at: 10 64 1489 1832 2451 10 Size: 54 1425 343 619 4586 Fragments arranged by size: 4586 1425 619 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2837 3318 3531 4985 5344 5503 518 Size: 648 1671 481 213 1454 359 159 2042 Fragments arranged by size: 2042 1671 1454 648 481 359 213 159 MspA1I CmG'CkG Cuts at: 1606 4204 4443 5120 6268 6513 1606 Size: 2598 239 677 1148 245 2120 Fragments arranged by size: 2598 2120 1148 677 245 239 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 6475 NdeI CA'TA_TG Cuts at: 387 387 Size: 7027 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7027 NotI GC'GGCC_GC Cuts at: 4474 4474 Size: 7027 NspI r_CATG'y Cuts at: 1132 1229 3536 3822 4385 5930 1132 Size: 97 2307 286 563 1545 2229 Fragments arranged by size: 2307 2229 1545 563 286 97 PasI CC'CwG_GG Cuts at: 1348 2154 3907 1348 Size: 806 1753 4468 Fragments arranged by size: 4468 1753 806 PciI A'CATG_T Cuts at: 5926 5926 Size: 7027 PflMI CCAn_nnn'nTGG Cuts at: 1108 2716 1108 Size: 1608 5419 PfoI T'CCnGG_A Cuts at: 2144 2709 2144 Size: 565 6462 PmeI GTTT'AAAC Cuts at: 4331 4331 Size: 7027 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5096 5128 6609 6641 5096 Size: 32 1481 32 5482 Fragments arranged by size: 5482 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2118 2713 2118 Size: 595 6432 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7027 PsiI TTA'TAA Cuts at: 4154 4597 4154 Size: 443 6584 PstI C_TGCA'G Cuts at: 830 3542 4379 830 Size: 2712 837 3478 Fragments arranged by size: 3478 2712 837 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7027 PvuII CAG'CTG Cuts at: 4204 4204 Size: 7027 SalI G'TCGA_C Cuts at: 1936 4369 1936 Size: 2433 4594 SbfI CC_TGCA'GG Cuts at: 4379 4379 Size: 7027 ScaI AGT'ACT Cuts at: 5275 6987 5275 Size: 1712 5315 SfcI C'TryA_G Cuts at: 826 1045 3538 4375 5510 6191 6382 826 Size: 219 2493 837 1135 681 191 1471 Fragments arranged by size: 2493 1471 1135 837 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7027 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7027 SmaI CCC'GGG Cuts at: 1872 2097 4354 1872 Size: 225 2257 4545 Fragments arranged by size: 4545 2257 225 SmlI C'TyrA_G Cuts at: 820 1017 1943 2494 3782 5131 6032 6294 820 Size: 197 926 551 1288 1349 901 262 1553 Fragments arranged by size: 1553 1349 1288 926 901 551 262 197 SnaBI TAC'GTA Cuts at: 493 4324 4818 493 Size: 3831 494 2702 Fragments arranged by size: 3831 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7027 SphI G_CATG'C Cuts at: 1132 4385 1132 Size: 3253 3774 SspI AAT'ATT Cuts at: 5 52 4089 4951 5 Size: 47 4037 862 2081 Fragments arranged by size: 4037 2081 862 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 7027 StyI C'CwwG_G Cuts at: 513 1065 3606 4143 4466 513 Size: 552 2541 537 323 3074 Fragments arranged by size: 3074 2541 552 537 323 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2593 3004 3384 3696 5054 5071 5224 Size: 1255 1013 411 380 312 1358 17 153 Cuts at: 5224 5409 5854 325 Size: 185 445 1498 Fragments arranged by size: 1498 1358 1255 1013 445 411 380 312 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2626 2867 3626 Size: 274 80 33 51 1443 649 241 759 Cuts at: 3626 5273 6985 96 Size: 1647 1712 138 Fragments arranged by size: 1712 1647 1443 759 649 274 241 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6987 7019 6987 Size: 32 6995 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7027 XbaI T'CTAG_A Cuts at: 4363 4363 Size: 7027 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2039 2486 2039 Size: 447 6580 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7027 XmaI C'CCGG_G Cuts at: 1870 2095 4352 1870 Size: 225 2257 4545 Fragments arranged by size: 4545 2257 225 XmnI GAAnn'nnTTC Cuts at: 5156 5156 Size: 7027 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4835 276 Size: 53 83 186 936 3301 2468 Fragments arranged by size: 3301 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI