(Circular) (Six-base) MAPSORT of: pFN21ASDA0135.seq Check: 6286 from: 1 to: 8719 pFN21ASDA0135 8719 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3255 6529 278 Size: 53 83 186 936 1719 3274 2468 Fragments arranged by size: 3274 2468 1719 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4744 6062 1937 Size: 2807 1318 4594 Fragments arranged by size: 4594 2807 1318 Acc65I G'GTAC_C Cuts at: 2939 6040 2939 Size: 3101 5618 AclI AA'CG_TT Cuts at: 1563 6846 7219 1563 Size: 5283 373 3063 Fragments arranged by size: 5283 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2238 3690 6782 8166 8434 1632 Size: 606 1452 3092 1384 268 1917 Fragments arranged by size: 3092 1917 1452 1384 606 268 AfeI AGC'GCT Cuts at: 2211 2211 Size: 8719 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8522 AflIII A'CryG_T Cuts at: 1203 2811 4558 4593 7618 8595 1203 Size: 1608 1747 35 3025 977 1327 Fragments arranged by size: 3025 1747 1608 1327 977 35 AgeI A'CCGG_T Cuts at: 2999 7550 2999 Size: 4551 4168 AhdI GACnn_n'nnGTC Cuts at: 4285 7448 4285 Size: 3163 5556 AleI CACnn'nnGTG Cuts at: 1166 2621 4646 1166 Size: 1455 2025 5239 Fragments arranged by size: 5239 2025 1455 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2307 3573 1184 Size: 1123 1266 6330 Fragments arranged by size: 6330 1266 1123 AlwNI CAG_nnn'CTG Cuts at: 2193 2506 2549 2663 3535 4944 5750 6462 8034 Size: 313 43 114 872 1409 806 712 1572 Cuts at: 8034 2193 Size: 2878 Fragments arranged by size: 2878 1572 1409 872 806 712 313 114 43 ApaI G_GGCC'C Cuts at: 2108 4781 5945 2108 Size: 2673 1164 4882 Fragments arranged by size: 4882 2673 1164 ApaLI G'TGCA_C Cuts at: 3518 5550 6776 7932 3518 Size: 2032 1226 1156 4305 Fragments arranged by size: 4305 2032 1226 1156 ApoI r'AATT_y Cuts at: 1493 4231 6028 6254 6406 8515 1493 Size: 2738 1797 226 152 2109 1697 Fragments arranged by size: 2738 2109 1797 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 3339 7273 160 Size: 872 2307 3934 1606 Fragments arranged by size: 3934 2307 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8719 AvaI C'yCGr_G Cuts at: 1870 1943 3372 5987 6044 1870 Size: 73 1429 2615 57 4545 Fragments arranged by size: 4545 2615 1429 73 57 BamHI G'GATC_C Cuts at: 3328 5149 5992 6049 6416 3328 Size: 1821 843 57 367 5631 Fragments arranged by size: 5631 1821 843 367 57 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2765 2939 4427 4697 6040 Size: 325 217 627 978 174 1488 270 1343 Cuts at: 6040 7495 618 Size: 1455 1842 Fragments arranged by size: 1842 1488 1455 1343 978 627 325 270 217 174 BanII G_rGCy'C Cuts at: 1391 2108 2133 2234 2484 2724 3150 3596 4045 Size: 717 25 101 250 240 426 446 449 Cuts at: 4045 4352 4773 4781 4905 4959 5033 5331 5945 Size: 307 421 8 124 54 74 298 614 Cuts at: 5945 6038 1391 Size: 93 4072 Fragments arranged by size: 4072 717 614 449 446 426 421 307 298 250 240 124 101 93 74 54 25 8 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2226 928 Size: 971 327 7421 Fragments arranged by size: 7421 971 327 BbvCI CC'TCA_GC Cuts at: 2149 2567 3315 3471 3687 4159 5454 2149 Size: 418 748 156 216 472 1295 5414 Fragments arranged by size: 5414 1295 748 472 418 216 156 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3912 3946 5531 5565 6910 6944 1903 Size: 34 1975 34 1585 34 1345 34 3678 Fragments arranged by size: 3678 1975 1585 1345 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5387 6611 7821 5387 Size: 1224 1210 6285 Fragments arranged by size: 6285 1224 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 8719 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4875 5037 7330 Size: 107 122 71 631 605 3203 162 2293 Cuts at: 7330 136 Size: 1525 Fragments arranged by size: 3203 2293 1525 631 605 162 122 107 71 BglII A'GATC_T Cuts at: 8714 8714 Size: 8719 BlpI GC'TnA_GC Cuts at: 2127 4899 6136 2127 Size: 2772 1237 4710 Fragments arranged by size: 4710 2772 1237 Bme1580I G_kGCm'C Cuts at: 1790 2108 3372 3522 4601 4781 5554 5945 6780 Size: 318 1264 150 1079 180 773 391 835 Cuts at: 6780 7936 1790 Size: 1156 2573 Fragments arranged by size: 2573 1264 1156 1079 835 773 391 318 180 150 BmgBI CAC'GTC Cuts at: 1318 2775 2827 1318 Size: 1457 52 7210 Fragments arranged by size: 7210 1457 52 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4018 6450 7408 Size: 416 53 83 399 24 2601 2432 958 Cuts at: 7408 442 Size: 1753 Fragments arranged by size: 2601 2432 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8719 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2752 3467 4348 2752 Size: 715 881 7123 Fragments arranged by size: 7123 881 715 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2738 3462 3465 3660 4358 4757 4997 5615 Size: 1499 724 3 195 698 399 240 618 Cuts at: 5615 5681 5906 7379 1239 Size: 66 225 1473 2579 Fragments arranged by size: 2579 1499 1473 724 698 618 399 240 225 195 66 3 Bpu10I CC'TnA_GC Cuts at: 2149 2165 2301 2567 3315 3471 3687 4159 5454 Size: 16 136 266 748 156 216 472 1295 Cuts at: 5454 5925 2149 Size: 471 4943 Fragments arranged by size: 4943 1295 748 472 471 266 216 156 136 16 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2888 4235 4520 6844 7709 8007 2888 Size: 1347 285 2324 865 298 3600 Fragments arranged by size: 3600 2324 1347 865 298 285 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2740 4122 5610 5660 7382 882 Size: 617 1241 1382 1488 50 1722 2219 Fragments arranged by size: 2219 1722 1488 1382 1241 617 50 BsaAI yAC'GTr Cuts at: 493 1204 2812 4596 6016 6510 493 Size: 711 1608 1784 1420 494 2702 Fragments arranged by size: 2702 1784 1608 1420 711 494 BsaBI GATnn'nnATC Cuts at: 8713 8713 Size: 8719 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3252 6526 6908 7543 Size: 53 83 186 936 1719 3274 382 635 Cuts at: 7543 275 Size: 1451 Fragments arranged by size: 3274 1719 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2999 3384 4907 5554 7152 7550 7824 7971 8382 Size: 385 1523 647 1598 398 274 147 411 Cuts at: 8382 2999 Size: 3336 Fragments arranged by size: 3336 1598 1523 647 411 398 385 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 4004 4034 5588 5618 5893 Size: 30 165 30 2601 30 1554 30 275 Cuts at: 5893 5923 1178 Size: 30 3974 Fragments arranged by size: 3974 2601 1554 275 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2588 3002 4147 4625 4808 4967 5654 5678 Size: 1402 414 1145 478 183 159 687 24 Cuts at: 5678 1186 Size: 4227 Fragments arranged by size: 4227 1402 1145 687 478 414 183 159 24 BseYI C'CCAG_C Cuts at: 2107 2132 2831 2978 3310 3682 3735 3764 3785 Size: 25 699 147 332 372 53 29 21 Cuts at: 3785 3905 3964 4913 5035 7922 8418 2107 Size: 120 59 949 122 2887 496 2408 Fragments arranged by size: 2887 2408 949 699 496 372 332 147 122 120 59 53 29 25 21 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2097 2267 2885 3034 4129 1163 Size: 573 361 170 618 149 1095 5753 Fragments arranged by size: 5753 1095 618 573 361 170 149 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3982 5308 5493 6169 6930 7958 Size: 275 186 1981 1326 185 676 761 1028 Cuts at: 7958 1540 Size: 2301 Fragments arranged by size: 2301 1981 1326 1028 761 676 275 186 185 BsiHKAI G_wGCw'C Cuts at: 2234 2307 2580 3150 3522 4773 4905 4932 5205 Size: 73 273 570 372 1251 132 27 273 Cuts at: 5205 5554 6780 6865 7936 2234 Size: 349 1226 85 1071 3017 Fragments arranged by size: 3017 1251 1226 1071 570 372 349 273 273 132 85 73 27 BsmI GAATG_Cn' Cuts at: 6230 6323 6230 Size: 93 8626 BsmBI CGTCTCn'nnnn_ Cuts at: 4719 5749 4719 Size: 1030 7689 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2108 2133 2234 2307 2484 2580 2724 Size: 399 318 25 101 73 177 96 144 Cuts at: 2724 3150 3372 3522 3596 4045 4352 4601 4773 Size: 426 222 150 74 449 307 249 172 Cuts at: 4773 4781 4905 4932 4959 5033 5205 5331 5554 Size: 8 124 27 27 74 172 126 223 Cuts at: 5554 5945 6038 6780 6865 7936 1391 Size: 391 93 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 449 426 399 391 318 307 249 223 222 177 172 172 150 144 126 124 101 96 93 85 74 74 73 27 27 25 8 BspEI T'CCGG_A Cuts at: 3384 8382 3384 Size: 4998 3721 BspHI T'CATG_A Cuts at: 5715 6608 8338 5715 Size: 893 1730 6096 Fragments arranged by size: 6096 1730 893 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2127 3612 4007 5406 6074 844 Size: 1283 1485 395 1399 668 3489 Fragments arranged by size: 3489 1485 1399 1283 668 395 BsrBI CCG'CTC Cuts at: 1394 1823 3021 3745 4097 6606 1394 Size: 429 1198 724 352 2509 3507 Fragments arranged by size: 3507 2509 1198 724 429 352 BsrDI GCAATG_nn' Cuts at: 65 1248 3265 7214 7388 65 Size: 1183 2017 3949 174 1396 Fragments arranged by size: 3949 2017 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2999 4424 7363 7550 1700 Size: 110 1189 1425 2939 187 2869 Fragments arranged by size: 2939 2869 1425 1189 187 110 BsrGI T'GTAC_A Cuts at: 96 2270 5622 96 Size: 2174 3352 3193 Fragments arranged by size: 3352 3193 2174 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8719 BssSI C'ACGA_G Cuts at: 6779 7791 6779 Size: 1012 7707 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8719 BstBI TT'CG_AA Cuts at: 2007 7523 2007 Size: 5516 3203 BstEII G'GTnAC_C Cuts at: 1184 4633 1184 Size: 3449 5270 BstXI CCAn_nnnn'nTGG Cuts at: 2762 3247 4921 4969 2762 Size: 485 1674 48 6512 Fragments arranged by size: 6512 1674 485 48 BstYI r'GATC_y Cuts at: 1972 2451 3328 3381 3604 4686 5149 5992 6049 Size: 479 877 53 223 1082 463 843 57 Cuts at: 6049 6416 6801 6818 8270 8356 8368 8714 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1082 877 843 479 463 385 367 346 223 86 57 53 17 12 BstZ17I GTA'TAC Cuts at: 4745 4745 Size: 8719 Bsu36I CC'TnA_GG Cuts at: 2368 4587 2368 Size: 2219 6500 BtgI C'CryG_G Cuts at: 513 1065 2022 2324 2724 3094 3293 3795 3959 Size: 552 957 302 400 370 199 502 164 Cuts at: 3959 4179 5670 5789 513 Size: 220 1491 119 3443 Fragments arranged by size: 3443 1491 957 552 502 400 370 302 220 199 164 119 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2775 487 Size: 683 56 1549 6431 Fragments arranged by size: 6431 1549 683 56 BtsI GCAGTG_nn' Cuts at: 2121 2284 2338 3555 3706 3847 4212 4350 5047 Size: 163 54 1217 151 141 365 138 697 Cuts at: 5047 5394 6235 7029 7049 2121 Size: 347 841 794 20 3791 Fragments arranged by size: 3791 1217 841 794 697 365 347 163 151 141 138 54 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8719 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3247 3282 529 Size: 35 2683 35 5966 Fragments arranged by size: 5966 2683 35 35 DraI TTT'AAA Cuts at: 6023 6023 Size: 8719 DraIII CAC_nnn'GTG Cuts at: 3367 3367 Size: 8719 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 7726 809 Size: 507 6410 1802 Fragments arranged by size: 6410 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2512 2593 2881 Size: 54 1425 50 275 18 682 81 288 Cuts at: 2881 3237 3358 3798 4176 4444 5490 5968 6166 Size: 356 121 440 378 268 1046 478 198 Cuts at: 6166 7055 8684 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1046 889 682 478 440 378 356 288 275 268 198 121 81 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5490 6166 1537 Size: 275 3678 676 4090 Fragments arranged by size: 4090 3678 676 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2046 2669 2920 3981 4339 5297 5826 6649 Size: 693 623 251 1061 358 958 529 823 Cuts at: 6649 1353 Size: 3423 Fragments arranged by size: 3423 1061 958 823 693 623 529 358 251 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 7306 7680 7826 387 Size: 1064 138 5717 374 146 1280 Fragments arranged by size: 5717 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2232 3148 4771 4903 2232 Size: 916 1623 132 6048 Fragments arranged by size: 6048 1623 916 132 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2238 2738 3462 3465 3660 3690 4358 Size: 393 606 500 724 3 195 30 668 Cuts at: 4358 4757 4997 5615 5681 5906 6782 7379 8166 Size: 399 240 618 66 225 876 597 787 Cuts at: 8166 8434 1239 Size: 268 1524 Fragments arranged by size: 1524 876 787 724 668 618 606 597 500 399 393 268 240 225 195 66 30 3 EcoNI CCTnn'n_nnAGG Cuts at: 2570 3480 2570 Size: 910 7809 EcoO109I rG'GnC_Cy Cuts at: 2104 2503 2563 3307 4195 4777 4778 4848 5046 Size: 399 60 744 888 582 1 70 198 Cuts at: 5046 5941 5942 2104 Size: 895 1 4881 Fragments arranged by size: 4881 895 888 744 582 399 198 70 60 1 1 EcoRI G'AATT_C Cuts at: 4231 6028 6406 4231 Size: 1797 378 6544 Fragments arranged by size: 6544 1797 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2440 4276 2440 Size: 1836 6883 FspI TGC'GCA Cuts at: 7225 7225 Size: 8719 HaeII r_GCGC'y Cuts at: 1678 2213 2695 3536 7866 1678 Size: 535 482 841 4330 2531 Fragments arranged by size: 4330 2531 841 535 482 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3366 3398 3931 3963 4004 Size: 32 135 32 1794 32 533 32 41 Cuts at: 4004 4036 4271 4303 5134 5166 5471 5503 5588 Size: 32 235 32 831 32 305 32 85 Cuts at: 5588 5590 5620 5622 7360 7392 7434 7466 1373 Size: 2 30 2 1738 32 42 32 2626 Fragments arranged by size: 2626 1794 1738 831 533 305 235 135 85 42 41 32 32 32 32 32 32 32 32 32 32 30 2 2 HincII GTy'rAC Cuts at: 669 1657 1938 4438 6063 6309 669 Size: 988 281 2500 1625 246 3079 Fragments arranged by size: 3079 2500 1625 988 281 246 HindIII A'AGCT_T Cuts at: 748 2472 748 Size: 1724 6995 HpaI GTT'AAC Cuts at: 6309 6309 Size: 8719 KpnI G_GTAC'C Cuts at: 2943 6044 2943 Size: 3101 5618 MfeI C'AATT_G Cuts at: 6318 6318 Size: 8719 MluI A'CGCG_T Cuts at: 8595 8595 Size: 8719 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2902 4097 4511 4554 5120 5374 7833 8017 Size: 1597 1195 414 43 566 254 2459 184 Cuts at: 8017 1305 Size: 2007 Fragments arranged by size: 2459 2007 1597 1195 566 414 254 184 43 MscI TGG'CCA Cuts at: 10 64 1489 1832 2514 2883 3239 3360 3800 Size: 54 1425 343 682 369 356 121 440 Cuts at: 3800 4178 4446 5970 10 Size: 378 268 1524 2759 Fragments arranged by size: 2759 1524 1425 682 440 378 369 356 343 268 121 54 MslI CAynn'nnrTG Cuts at: 518 1166 2621 4646 6677 7036 7195 518 Size: 648 1455 2025 2031 359 159 2042 Fragments arranged by size: 2042 2031 2025 1455 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2111 2326 3243 3314 3686 3768 3823 4018 Size: 505 215 917 71 372 82 55 195 Cuts at: 4018 4681 4895 5035 5876 6135 6812 7960 8205 Size: 663 214 140 841 259 677 1148 245 Cuts at: 8205 1606 Size: 2120 Fragments arranged by size: 2120 1148 917 841 677 663 505 372 259 245 215 214 195 140 82 71 55 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 7210 Fragments arranged by size: 7210 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 8719 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8719 NotI GC'GGCC_GC Cuts at: 6166 6166 Size: 8719 NspI r_CATG'y Cuts at: 1132 1229 3715 6077 7622 1132 Size: 97 2486 2362 1545 2229 Fragments arranged by size: 2486 2362 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 4190 1348 Size: 2842 5877 PciI A'CATG_T Cuts at: 7618 7618 Size: 8719 PflMI CCAn_nnn'nTGG Cuts at: 1108 4944 5861 1108 Size: 3836 917 3966 Fragments arranged by size: 3966 3836 917 PfoI T'CCnGG_A Cuts at: 2715 4241 4374 4395 4461 4689 4706 2715 Size: 1526 133 21 66 228 17 6728 Fragments arranged by size: 6728 1526 228 133 66 21 17 PmeI GTTT'AAAC Cuts at: 6023 6023 Size: 8719 PmlI CAC'GTG Cuts at: 4596 4596 Size: 8719 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6788 6820 8301 8333 6788 Size: 32 1481 32 7174 Fragments arranged by size: 7174 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2503 3307 4848 2503 Size: 804 1541 6374 Fragments arranged by size: 6374 1541 804 PshAI GACnn'nnGTC Cuts at: 1613 2744 1613 Size: 1131 7588 PsiI TTA'TAA Cuts at: 6289 6289 Size: 8719 PspOMI G'GGCC_C Cuts at: 2104 4777 5941 2104 Size: 2673 1164 4882 Fragments arranged by size: 4882 2673 1164 PstI C_TGCA'G Cuts at: 830 3996 4680 5742 6071 830 Size: 3166 684 1062 329 3478 Fragments arranged by size: 3478 3166 1062 684 329 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8719 PvuII CAG'CTG Cuts at: 2111 3243 3314 3686 3823 4018 2111 Size: 1132 71 372 137 195 6812 Fragments arranged by size: 6812 1132 372 195 137 71 SacI G_AGCT'C Cuts at: 2234 3150 4773 4905 2234 Size: 916 1623 132 6048 Fragments arranged by size: 6048 1623 916 132 SacII CC_GC'GG Cuts at: 2327 2327 Size: 8719 SalI G'TCGA_C Cuts at: 1936 6061 1936 Size: 4125 4594 SapI GCTCTTCn'nnn_ Cuts at: 2920 3981 2920 Size: 1061 7658 SbfI CC_TGCA'GG Cuts at: 6071 6071 Size: 8719 ScaI AGT'ACT Cuts at: 5006 5546 6967 8679 5006 Size: 540 1421 1712 5046 Fragments arranged by size: 5046 1712 1421 540 SexAI A'CCwGG_T Cuts at: 5697 5697 Size: 8719 SfcI C'TryA_G Cuts at: 826 1045 3187 3992 4676 5578 5738 6067 7202 Size: 219 2142 805 684 902 160 329 1135 Cuts at: 7202 7883 8074 826 Size: 681 191 1471 Fragments arranged by size: 2142 1471 1135 902 805 684 681 329 219 191 160 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8719 SgrAI Cr'CCGG_yG Cuts at: 1700 2999 1700 Size: 1299 7420 SmaI CCC'GGG Cuts at: 1872 5989 6046 1872 Size: 4117 57 4545 Fragments arranged by size: 4545 4117 57 SmlI C'TyrA_G Cuts at: 820 1017 1943 2903 4250 4499 6823 7724 7986 Size: 197 926 960 1347 249 2324 901 262 Cuts at: 7986 820 Size: 1553 Fragments arranged by size: 2324 1553 1347 960 926 901 262 249 197 SnaBI TAC'GTA Cuts at: 493 6016 6510 493 Size: 5523 494 2702 Fragments arranged by size: 5523 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8719 SphI G_CATG'C Cuts at: 1132 6077 1132 Size: 4945 3774 SspI AAT'ATT Cuts at: 5 52 2266 6643 5 Size: 47 2214 4377 2081 Fragments arranged by size: 4377 2214 2081 47 StuI AGG'CCT Cuts at: 1517 3927 1517 Size: 2410 6309 StyI C'CwwG_G Cuts at: 513 1065 2022 3285 3332 3540 3561 3813 4173 Size: 552 957 1263 47 208 21 252 360 Cuts at: 4173 4338 4441 4447 4616 4962 5242 5766 5971 Size: 165 103 6 169 346 280 524 205 Cuts at: 5971 6158 513 Size: 187 3074 Fragments arranged by size: 3074 1263 957 552 524 360 346 280 252 208 205 187 169 165 103 47 21 6 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3031 3258 3996 4898 6746 6763 6916 Size: 1255 1451 227 738 902 1848 17 153 Cuts at: 6916 7101 7546 325 Size: 185 445 1498 Fragments arranged by size: 1848 1498 1451 1255 902 738 445 227 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2270 5004 5016 Size: 274 80 33 51 1443 293 2734 12 Cuts at: 5016 5544 5622 6965 8677 96 Size: 528 78 1343 1712 138 Fragments arranged by size: 2734 1712 1443 1343 528 293 274 138 80 78 51 33 12 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4550 4582 5822 5854 5893 5925 8679 8711 4550 Size: 32 1240 32 39 32 2754 32 4558 Fragments arranged by size: 4558 2754 1240 39 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3494 1316 Size: 2178 6541 XbaI T'CTAG_A Cuts at: 3949 6055 3949 Size: 2106 6613 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2428 2507 2664 3820 5032 2428 Size: 79 157 1156 1212 6115 Fragments arranged by size: 6115 1212 1156 157 79 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 8719 XmaI C'CCGG_G Cuts at: 1870 5987 6044 1870 Size: 4117 57 4545 Fragments arranged by size: 4545 4117 57 XmnI GAAnn'nnTTC Cuts at: 4068 5648 6848 4068 Size: 1580 1200 5939 Fragments arranged by size: 5939 1580 1200 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3253 6527 276 Size: 53 83 186 936 1719 3274 2468 Fragments arranged by size: 3274 2468 1719 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspXI PsrI RsrII SanDI SfiI SfoI SrfI SwaI