(Circular) (Six-base) MAPSORT of: pFN21ASDA0127.seq Check: 6274 from: 1 to: 5671 pFN21ASDA0127 5671 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2723 3481 278 Size: 53 83 186 936 1187 758 2468 Fragments arranged by size: 2468 1187 936 758 186 83 53 AccI GT'mk_AC Cuts at: 1937 3014 1937 Size: 1077 4594 Acc65I G'GTAC_C Cuts at: 2992 2992 Size: 5671 AclI AA'CG_TT Cuts at: 1563 3798 4171 1563 Size: 2235 373 3063 Fragments arranged by size: 3063 2235 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2326 3734 5118 5386 1632 Size: 694 1408 1384 268 1917 Fragments arranged by size: 1917 1408 1384 694 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5474 AflIII A'CryG_T Cuts at: 1203 2223 2445 4570 5547 1203 Size: 1020 222 2125 977 1327 Fragments arranged by size: 2125 1327 1020 977 222 AgeI A'CCGG_T Cuts at: 4502 4502 Size: 5671 AhdI GACnn_n'nnGTC Cuts at: 4400 4400 Size: 5671 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5671 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5671 AlwNI CAG_nnn'CTG Cuts at: 3414 4986 3414 Size: 1572 4099 ApaLI G'TGCA_C Cuts at: 3728 4884 3728 Size: 1156 4515 ApoI r'AATT_y Cuts at: 1493 2704 2980 3206 3358 5467 1493 Size: 1211 276 226 152 2109 1697 Fragments arranged by size: 2109 1697 1211 276 226 152 AseI AT'TA_AT Cuts at: 160 1032 4225 160 Size: 872 3193 1606 Fragments arranged by size: 3193 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5671 AvaI C'yCGr_G Cuts at: 1870 1943 2429 2996 1870 Size: 73 486 567 4545 Fragments arranged by size: 4545 567 486 73 BamHI G'GATC_C Cuts at: 2235 3001 3368 2235 Size: 766 367 4538 Fragments arranged by size: 4538 766 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2633 2992 4447 618 Size: 325 217 627 846 359 1455 1842 Fragments arranged by size: 1842 1455 846 627 359 325 217 BanII G_rGCy'C Cuts at: 1391 2193 2310 2559 2990 1391 Size: 802 117 249 431 4072 Fragments arranged by size: 4072 802 431 249 117 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4700 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3862 3896 1903 Size: 34 1925 34 3678 Fragments arranged by size: 3678 1925 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3563 4773 3563 Size: 1210 4461 BclI T'GATC_A Cuts at: 1548 1548 Size: 5671 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2336 4282 136 Size: 107 122 71 631 605 664 1946 1525 Fragments arranged by size: 1946 1525 664 631 605 122 107 71 BglII A'GATC_T Cuts at: 5666 5666 Size: 5671 BlpI GC'TnA_GC Cuts at: 3088 3088 Size: 5671 Bme1580I G_kGCm'C Cuts at: 1790 3732 4888 1790 Size: 1942 1156 2573 Fragments arranged by size: 2573 1942 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5671 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3402 4360 442 Size: 416 53 83 399 24 1985 958 1753 Fragments arranged by size: 1985 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5671 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2420 2484 2605 4331 1239 Size: 1181 64 121 1726 2579 Fragments arranged by size: 2579 1726 1181 121 64 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2640 3796 4661 4959 2640 Size: 1156 865 298 3352 Fragments arranged by size: 3352 1156 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4334 882 Size: 617 2835 2219 Fragments arranged by size: 2835 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2968 3462 493 Size: 711 1764 494 2702 Fragments arranged by size: 2702 1764 711 494 BsaBI GATnn'nnATC Cuts at: 5665 5665 Size: 5671 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2354 2720 3478 3860 Size: 53 83 186 936 821 366 758 382 Cuts at: 3860 4495 275 Size: 635 1451 Fragments arranged by size: 1451 936 821 758 635 382 366 186 83 53 BsaWI w'CCGG_w Cuts at: 4104 4502 4776 4923 5334 4104 Size: 398 274 147 411 4441 Fragments arranged by size: 4441 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2513 2543 2857 2887 1178 Size: 30 165 30 1110 30 314 30 3962 Fragments arranged by size: 3962 1110 314 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2278 2521 2567 2849 1186 Size: 1092 243 46 282 4008 Fragments arranged by size: 4008 1092 282 243 46 BseYI C'CCAG_C Cuts at: 2192 2292 2309 4874 5370 2192 Size: 100 17 2565 496 2493 Fragments arranged by size: 2565 2493 496 100 17 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2788 1163 Size: 573 1052 4046 Fragments arranged by size: 4046 1052 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3121 3882 4910 1540 Size: 275 186 1120 761 1028 2301 Fragments arranged by size: 2301 1120 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2559 3732 3817 4888 2559 Size: 1173 85 1071 3342 Fragments arranged by size: 3342 1173 1071 85 BsmI GAATG_Cn' Cuts at: 3182 3275 3182 Size: 93 5578 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2193 2310 2559 2990 3732 3817 4888 Size: 399 403 117 249 431 742 85 1071 Cuts at: 4888 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 431 403 399 249 117 85 BspEI T'CCGG_A Cuts at: 5334 5334 Size: 5671 BspHI T'CATG_A Cuts at: 3560 5290 3560 Size: 1730 3941 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3026 844 Size: 2182 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3558 1394 Size: 429 1735 3507 Fragments arranged by size: 3507 1735 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4166 4340 65 Size: 1183 2918 174 1396 Fragments arranged by size: 2918 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2335 4315 4502 1700 Size: 110 525 1980 187 2869 Fragments arranged by size: 2869 1980 525 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5671 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5671 BssSI C'ACGA_G Cuts at: 3731 4743 3731 Size: 1012 4659 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5671 BstBI TT'CG_AA Cuts at: 2007 4475 2007 Size: 2468 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5671 BstYI r'GATC_y Cuts at: 1972 2235 3001 3368 3753 3770 5222 5308 5320 Size: 263 766 367 385 17 1452 86 12 Cuts at: 5320 5666 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 766 385 367 346 263 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2267 2267 Size: 5671 BtgI C'CryG_G Cuts at: 513 1065 2475 2589 2724 513 Size: 552 1410 114 135 3460 Fragments arranged by size: 3460 1410 552 135 114 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4932 Fragments arranged by size: 4932 683 56 BtsI GCAGTG_nn' Cuts at: 2423 3187 3981 4001 2423 Size: 764 794 20 4093 Fragments arranged by size: 4093 794 764 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5671 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5636 DraI TTT'AAA Cuts at: 2975 2975 Size: 5671 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4678 809 Size: 507 3362 1802 Fragments arranged by size: 3362 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2586 3118 4007 Size: 54 1425 50 275 18 756 532 889 Cuts at: 4007 5636 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 756 532 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3118 1537 Size: 275 1306 4090 Fragments arranged by size: 4090 1306 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3601 1353 Size: 2248 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2248 4258 4632 4778 387 Size: 1064 138 659 2010 374 146 1280 Fragments arranged by size: 2010 1280 1064 659 374 146 138 EcoICRI GAG'CTC Cuts at: 2557 2557 Size: 5671 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2326 2420 2484 2605 3734 4331 5118 Size: 393 694 94 64 121 1129 597 787 Cuts at: 5118 5386 1239 Size: 268 1524 Fragments arranged by size: 1524 1129 787 694 597 393 268 121 94 64 EcoO109I rG'GnC_Cy Cuts at: 2178 2329 2616 2961 2178 Size: 151 287 345 4888 Fragments arranged by size: 4888 345 287 151 EcoRI G'AATT_C Cuts at: 2980 3358 2980 Size: 378 5293 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2925 2925 Size: 5671 FspI TGC'GCA Cuts at: 4177 4177 Size: 5671 HaeII r_GCGC'y Cuts at: 1678 2134 4818 1678 Size: 456 2684 2531 Fragments arranged by size: 2684 2531 456 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2090 2122 2513 2545 2677 Size: 32 135 32 518 32 391 32 132 Cuts at: 2677 2709 4312 4344 4386 4418 1373 Size: 32 1603 32 42 32 2626 Fragments arranged by size: 2626 1603 518 391 135 132 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3015 3261 669 Size: 988 281 1077 246 3079 Fragments arranged by size: 3079 1077 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5671 HpaI GTT'AAC Cuts at: 3261 3261 Size: 5671 KpnI G_GTAC'C Cuts at: 2996 2996 Size: 5671 MfeI C'AATT_G Cuts at: 3270 3270 Size: 5671 MluI A'CGCG_T Cuts at: 5547 5547 Size: 5671 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4785 4969 1305 Size: 3480 184 2007 Fragments arranged by size: 3480 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3849 Fragments arranged by size: 3849 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2064 2948 3629 3988 4147 518 Size: 648 898 884 681 359 159 2042 Fragments arranged by size: 2042 898 884 681 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3087 3764 4912 5157 1606 Size: 1481 677 1148 245 2120 Fragments arranged by size: 2120 1481 1148 677 245 NaeI GCC'GGC Cuts at: 2337 2337 Size: 5671 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5119 NdeI CA'TA_TG Cuts at: 387 387 Size: 5671 NgoMIV G'CCGG_C Cuts at: 2335 2335 Size: 5671 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5671 NotI GC'GGCC_GC Cuts at: 3118 3118 Size: 5671 NspI r_CATG'y Cuts at: 1132 1229 2227 3029 4574 1132 Size: 97 998 802 1545 2229 Fragments arranged by size: 2229 1545 998 802 97 PacI TTA_AT'TAA Cuts at: 2217 2217 Size: 5671 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5671 PciI A'CATG_T Cuts at: 2223 4570 2223 Size: 2347 3324 PflMI CCAn_nnn'nTGG Cuts at: 1108 2944 1108 Size: 1836 3835 PfoI T'CCnGG_A Cuts at: 2238 2238 Size: 5671 PmeI GTTT'AAAC Cuts at: 2975 2975 Size: 5671 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3740 3772 5253 5285 3740 Size: 32 1481 32 4126 Fragments arranged by size: 4126 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2616 2961 2616 Size: 345 5326 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5671 PsiI TTA'TAA Cuts at: 3241 3241 Size: 5671 PspXI vC'TCGA_Gb Cuts at: 2429 2429 Size: 5671 PstI C_TGCA'G Cuts at: 830 3023 830 Size: 2193 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5671 SacI G_AGCT'C Cuts at: 2559 2559 Size: 5671 SalI G'TCGA_C Cuts at: 1936 3013 1936 Size: 1077 4594 SbfI CC_TGCA'GG Cuts at: 3023 3023 Size: 5671 ScaI AGT'ACT Cuts at: 3919 5631 3919 Size: 1712 3959 SfcI C'TryA_G Cuts at: 826 1045 3019 4154 4835 5026 826 Size: 219 1974 1135 681 191 1471 Fragments arranged by size: 1974 1471 1135 681 219 191 SfiI GGCCn_nnn'nGGCC Cuts at: 2336 2336 Size: 5671 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5671 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5671 SmaI CCC'GGG Cuts at: 1872 2998 1872 Size: 1126 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2429 2655 3775 4676 4938 820 Size: 197 926 486 226 1120 901 262 1553 Fragments arranged by size: 1553 1120 926 901 486 262 226 197 SnaBI TAC'GTA Cuts at: 493 2968 3462 493 Size: 2475 494 2702 Fragments arranged by size: 2702 2475 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5671 SphI G_CATG'C Cuts at: 1132 3029 1132 Size: 1897 3774 SspI AAT'ATT Cuts at: 5 52 3595 5 Size: 47 3543 2081 Fragments arranged by size: 3543 2081 47 StuI AGG'CCT Cuts at: 1517 2405 1517 Size: 888 4783 StyI C'CwwG_G Cuts at: 513 1065 2109 2538 3110 513 Size: 552 1044 429 572 3074 Fragments arranged by size: 3074 1044 572 552 429 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2651 3698 3715 3868 4053 4498 325 Size: 1255 1071 1047 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1071 1047 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3917 5629 96 Size: 274 80 33 51 1443 1940 1712 138 Fragments arranged by size: 1940 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2339 2371 5631 5663 2339 Size: 32 3260 32 2347 Fragments arranged by size: 3260 2347 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5671 XbaI T'CTAG_A Cuts at: 3007 3007 Size: 5671 XhoI C'TCGA_G Cuts at: 1943 2429 1943 Size: 486 5185 XmaI C'CCGG_G Cuts at: 1870 2996 1870 Size: 1126 4545 XmnI GAAnn'nnTTC Cuts at: 3800 3800 Size: 5671 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2721 3479 276 Size: 53 83 186 936 1187 758 2468 Fragments arranged by size: 2468 1187 936 758 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PacI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SacI SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PmlI PspOMI PsrI PvuII RsrII SacII SanDI SapI SexAI SfoI SrfI SwaI XcmI