(Circular) (Six-base) MAPSORT of: pFN21ASDA0122.seq Check: 4642 from: 1 to: 7516 pFN21ASDA0122 7516 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3726 5326 278 Size: 53 83 186 936 2190 1600 2468 Fragments arranged by size: 2468 2190 1600 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3318 3599 4557 4859 1937 Size: 1381 281 958 302 4594 Fragments arranged by size: 4594 1381 958 302 281 Acc65I G'GTAC_C Cuts at: 3287 4837 3287 Size: 1550 5966 AclI AA'CG_TT Cuts at: 1563 5643 6016 1563 Size: 4080 373 3063 Fragments arranged by size: 4080 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2505 5579 6963 7231 1632 Size: 873 3074 1384 268 1917 Fragments arranged by size: 3074 1917 1384 873 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7319 AflIII A'CryG_T Cuts at: 1203 6415 7392 1203 Size: 5212 977 1327 Fragments arranged by size: 5212 1327 977 AgeI A'CCGG_T Cuts at: 6347 6347 Size: 7516 AhdI GACnn_n'nnGTC Cuts at: 3490 6245 3490 Size: 2755 4761 AleI CACnn'nnGTG Cuts at: 1166 2640 1166 Size: 1474 6042 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4628 1184 Size: 3444 4072 AlwNI CAG_nnn'CTG Cuts at: 2444 2761 2794 2827 3097 3106 3115 3835 4085 Size: 317 33 33 270 9 9 720 250 Cuts at: 4085 4094 4294 4553 5259 6831 2444 Size: 9 200 259 706 1572 3129 Fragments arranged by size: 3129 1572 720 706 317 270 259 250 200 33 33 9 9 9 ApaI G_GGCC'C Cuts at: 2980 3416 3997 4259 2980 Size: 436 581 262 6237 Fragments arranged by size: 6237 581 436 262 ApaLI G'TGCA_C Cuts at: 4719 5573 6729 4719 Size: 854 1156 5506 Fragments arranged by size: 5506 1156 854 ApoI r'AATT_y Cuts at: 1493 4825 5051 5203 7312 1493 Size: 3332 226 152 2109 1697 Fragments arranged by size: 3332 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 6070 160 Size: 872 5038 1606 Fragments arranged by size: 5038 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7516 AvaI C'yCGr_G Cuts at: 1870 1943 2242 2313 3458 4109 4502 4575 4841 Size: 73 299 71 1145 651 393 73 266 Cuts at: 4841 1870 Size: 4545 Fragments arranged by size: 4545 1145 651 393 299 266 73 73 71 BamHI G'GATC_C Cuts at: 3439 4846 5213 3439 Size: 1407 367 5742 Fragments arranged by size: 5742 1407 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2983 3287 3404 3910 4347 Size: 325 217 627 1196 304 117 506 437 Cuts at: 4347 4837 6292 618 Size: 490 1455 1842 Fragments arranged by size: 1842 1455 1196 627 506 490 437 325 304 217 117 BanII G_rGCy'C Cuts at: 1391 2249 2894 2980 3416 3636 3644 3997 4259 Size: 858 645 86 436 220 8 353 262 Cuts at: 4259 4368 4402 4518 4709 4835 1391 Size: 109 34 116 191 126 4072 Fragments arranged by size: 4072 858 645 436 353 262 220 191 126 116 109 86 34 8 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3366 928 Size: 971 1467 5078 Fragments arranged by size: 5078 1467 971 BbvCI CC'TCA_GC Cuts at: 2420 2420 Size: 7516 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2700 2734 4556 4590 5707 5741 1903 Size: 34 763 34 1822 34 1117 34 3678 Fragments arranged by size: 3678 1822 1117 763 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5408 6618 5408 Size: 1210 6306 BclI T'GATC_A Cuts at: 1548 1548 Size: 7516 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2178 2295 2304 Size: 107 122 71 631 605 506 117 9 Cuts at: 2304 3224 3254 6127 136 Size: 920 30 2873 1525 Fragments arranged by size: 2873 1525 920 631 605 506 122 117 107 71 30 9 BglII A'GATC_T Cuts at: 7511 7511 Size: 7516 BlpI GC'TnA_GC Cuts at: 2816 4933 2816 Size: 2117 5399 Bme1580I G_kGCm'C Cuts at: 1790 2739 2980 3368 3407 3416 3578 3913 3997 Size: 949 241 388 39 9 162 335 84 Cuts at: 3997 4259 4723 5577 6733 1790 Size: 262 464 854 1156 2573 Fragments arranged by size: 2573 1156 949 854 464 388 335 262 241 162 84 39 9 BmgBI CAC'GTC Cuts at: 1318 2039 1318 Size: 721 6795 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2810 3244 3796 Size: 416 53 83 399 24 1393 434 552 Cuts at: 3796 3850 4801 5247 6205 442 Size: 54 951 446 958 1753 Fragments arranged by size: 1753 1393 958 951 552 446 434 416 399 83 54 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7516 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2920 2920 Size: 7516 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3468 3620 3896 6176 1239 Size: 2229 152 276 2280 2579 Fragments arranged by size: 2579 2280 2229 276 152 Bpu10I CC'TnA_GC Cuts at: 2420 4260 2420 Size: 1840 5676 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3383 4403 5641 6506 6804 3383 Size: 1020 1238 865 298 4095 Fragments arranged by size: 4095 1238 1020 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2112 3740 6179 882 Size: 617 613 1628 2439 2219 Fragments arranged by size: 2439 2219 1628 617 613 BsaAI yAC'GTr Cuts at: 493 1204 4813 5307 493 Size: 711 3609 494 2702 Fragments arranged by size: 3609 2702 711 494 BsaBI GATnn'nnATC Cuts at: 7510 7510 Size: 7516 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2691 3723 4315 5323 Size: 53 83 186 936 1158 1032 592 1008 Cuts at: 5323 5705 6340 275 Size: 382 635 1451 Fragments arranged by size: 1451 1158 1032 1008 936 635 592 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5949 6347 6621 6768 7179 5949 Size: 398 274 147 411 6286 Fragments arranged by size: 6286 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2222 2252 3173 3203 3782 Size: 30 165 30 819 30 921 30 579 Cuts at: 3782 3812 1178 Size: 30 4882 Fragments arranged by size: 4882 921 819 579 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2222 2375 2378 2381 2384 2387 2390 2399 Size: 1036 153 3 3 3 3 3 9 Cuts at: 2399 2402 2405 2408 2995 3320 4250 4253 4283 Size: 3 3 3 587 325 930 3 30 Cuts at: 4283 4537 1186 Size: 254 4165 Fragments arranged by size: 4165 1036 930 587 325 254 153 30 9 3 3 3 3 3 3 3 3 3 BseYI C'CCAG_C Cuts at: 3090 3516 3990 4325 4585 6719 7215 3090 Size: 426 474 335 260 2134 496 3391 Fragments arranged by size: 3391 2134 496 474 426 335 260 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3101 4491 4757 1163 Size: 573 1365 1390 266 3922 Fragments arranged by size: 3922 1390 1365 573 266 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3318 3888 4180 4966 5727 6755 Size: 275 186 1317 570 292 786 761 1028 Cuts at: 6755 1540 Size: 2301 Fragments arranged by size: 2301 1317 1028 786 761 570 292 275 186 BsiHKAI G_wGCw'C Cuts at: 3624 3636 4126 4723 5577 5662 6733 3624 Size: 12 490 597 854 85 1071 4407 Fragments arranged by size: 4407 1071 854 597 490 85 12 BsmI GAATG_Cn' Cuts at: 5027 5120 5027 Size: 93 7423 BsmBI CGTCTCn'nnnn_ Cuts at: 2310 3730 2310 Size: 1420 6096 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2249 2739 2894 2980 3368 3407 3416 Size: 399 459 490 155 86 388 39 9 Cuts at: 3416 3578 3624 3636 3644 3913 3997 4126 4259 Size: 162 46 12 8 269 84 129 133 Cuts at: 4259 4368 4402 4518 4709 4723 4835 5577 5662 Size: 109 34 116 191 14 112 742 85 Cuts at: 5662 6733 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 490 459 399 388 269 191 162 155 133 129 116 112 109 86 85 84 46 39 34 14 12 9 8 BspEI T'CCGG_A Cuts at: 7179 7179 Size: 7516 BspHI T'CATG_A Cuts at: 5405 7135 5405 Size: 1730 5786 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3443 4871 844 Size: 2599 1428 3489 Fragments arranged by size: 3489 2599 1428 BsrBI CCG'CTC Cuts at: 1394 1823 2085 4264 5403 1394 Size: 429 262 2179 1139 3507 Fragments arranged by size: 3507 2179 1139 429 262 BsrDI GCAATG_nn' Cuts at: 65 1248 3237 6011 6185 65 Size: 1183 1989 2774 174 1396 Fragments arranged by size: 2774 1989 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2294 3313 3670 3776 4392 6160 6347 Size: 110 484 1019 357 106 616 1768 187 Cuts at: 6347 1700 Size: 2869 Fragments arranged by size: 2869 1768 1019 616 484 357 187 110 106 BsrGI T'GTAC_A Cuts at: 96 96 Size: 7516 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7516 BssSI C'ACGA_G Cuts at: 2773 5576 6588 2773 Size: 2803 1012 3701 Fragments arranged by size: 3701 2803 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2482 2761 2794 3106 3115 794 Size: 1688 279 33 312 9 5195 Fragments arranged by size: 5195 1688 312 279 33 9 BstBI TT'CG_AA Cuts at: 2007 6320 2007 Size: 4313 3203 BstEII G'GTnAC_C Cuts at: 1184 4741 4786 1184 Size: 3557 45 3914 Fragments arranged by size: 3914 3557 45 BstXI CCAn_nnnn'nTGG Cuts at: 3982 3982 Size: 7516 BstYI r'GATC_y Cuts at: 1972 3102 3439 4153 4846 5213 5598 5615 7067 Size: 1130 337 714 693 367 385 17 1452 Cuts at: 7067 7153 7165 7511 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1130 714 693 385 367 346 337 86 17 12 BstZ17I GTA'TAC Cuts at: 3600 3600 Size: 7516 BtgI C'CryG_G Cuts at: 513 1065 2022 2458 2958 3049 3388 4623 513 Size: 552 957 436 500 91 339 1235 3406 Fragments arranged by size: 3406 1235 957 552 500 436 339 91 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6777 Fragments arranged by size: 6777 683 56 BtsI GCAGTG_nn' Cuts at: 2792 3231 3576 3684 4083 4597 4769 5032 5826 Size: 439 345 108 399 514 172 263 794 Cuts at: 5826 5846 2792 Size: 20 4462 Fragments arranged by size: 4462 794 514 439 399 345 263 172 108 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7516 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2668 2703 529 Size: 35 2104 35 5342 Fragments arranged by size: 5342 2104 35 35 DraI TTT'AAA Cuts at: 4820 4820 Size: 7516 DraIII CAC_nnn'GTG Cuts at: 2482 2804 2482 Size: 322 7194 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2043 6523 809 Size: 507 727 4480 1802 Fragments arranged by size: 4480 1802 727 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2062 2461 2806 Size: 54 1425 50 275 18 232 399 345 Cuts at: 2806 3225 3885 4167 4963 5852 7481 8 Size: 419 660 282 796 889 1629 43 Fragments arranged by size: 1629 1425 889 796 660 419 399 345 282 275 232 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3885 4963 1537 Size: 275 2073 1078 4090 Fragments arranged by size: 4090 2073 1078 275 EarI CTCTTCn'nnn_ Cuts at: 1353 5446 1353 Size: 4093 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3871 6103 6477 6623 387 Size: 1064 138 2282 2232 374 146 1280 Fragments arranged by size: 2282 2232 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3634 3634 Size: 7516 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2505 3468 3620 3896 5579 6176 6963 Size: 393 873 963 152 276 1683 597 787 Cuts at: 6963 7231 1239 Size: 268 1524 Fragments arranged by size: 1683 1524 963 873 787 597 393 276 268 152 EcoNI CCTnn'n_nnAGG Cuts at: 2885 4194 2885 Size: 1309 6207 EcoO109I rG'GnC_Cy Cuts at: 2824 2976 2977 3112 3412 3413 3455 4255 4256 Size: 152 1 135 300 1 42 800 1 Cuts at: 4256 2824 Size: 6084 Fragments arranged by size: 6084 800 300 152 135 42 1 1 1 EcoRI G'AATT_C Cuts at: 4825 5203 4825 Size: 378 7138 FspI TGC'GCA Cuts at: 2936 6022 2936 Size: 3086 4430 HaeII r_GCGC'y Cuts at: 1678 4340 6663 1678 Size: 2662 2323 2531 Fragments arranged by size: 2662 2531 2323 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2186 2218 2767 2799 3173 Size: 32 135 32 614 32 549 32 374 Cuts at: 3173 3205 3476 3508 3734 3766 3782 3814 6157 Size: 32 271 32 226 32 16 32 2343 Cuts at: 6157 6189 6231 6263 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2343 614 549 374 271 226 135 42 32 32 32 32 32 32 32 32 32 32 16 HincII GTy'rAC Cuts at: 669 1657 1938 3319 4860 5106 669 Size: 988 281 1381 1541 246 3079 Fragments arranged by size: 3079 1541 1381 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7516 HpaI GTT'AAC Cuts at: 5106 5106 Size: 7516 KpnI G_GTAC'C Cuts at: 3291 4841 3291 Size: 1550 5966 MfeI C'AATT_G Cuts at: 5115 5115 Size: 7516 MluI A'CGCG_T Cuts at: 7392 7392 Size: 7516 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3033 4589 6630 6814 1305 Size: 1728 1556 2041 184 2007 Fragments arranged by size: 2041 2007 1728 1556 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2463 3227 4169 10 Size: 54 1425 343 631 764 942 3357 Fragments arranged by size: 3357 1425 942 764 631 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2190 2414 2640 4148 5474 5833 5992 Size: 648 1024 224 226 1508 1326 359 159 Cuts at: 5992 518 Size: 2042 Fragments arranged by size: 2042 1508 1326 1024 648 359 226 224 159 MspA1I CmG'CkG Cuts at: 1606 2467 3051 3094 3617 4218 4475 4644 4932 Size: 861 584 43 523 601 257 169 288 Cuts at: 4932 5609 6757 7002 1606 Size: 677 1148 245 2120 Fragments arranged by size: 2120 1148 861 677 601 584 523 288 257 245 169 43 NcoI C'CATG_G Cuts at: 513 1065 2022 2958 3388 4623 513 Size: 552 957 936 430 1235 3406 Fragments arranged by size: 3406 1235 957 936 552 430 NdeI CA'TA_TG Cuts at: 387 387 Size: 7516 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7516 NotI GC'GGCC_GC Cuts at: 4963 4963 Size: 7516 NruI TCG'CGA Cuts at: 2160 2160 Size: 7516 NspI r_CATG'y Cuts at: 1132 1229 4874 6419 1132 Size: 97 3645 1545 2229 Fragments arranged by size: 3645 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 2857 1348 Size: 1509 6007 PciI A'CATG_T Cuts at: 6415 6415 Size: 7516 PflMI CCAn_nnn'nTGG Cuts at: 1108 2804 4629 1108 Size: 1696 1825 3995 Fragments arranged by size: 3995 1825 1696 PmeI GTTT'AAAC Cuts at: 4820 4820 Size: 7516 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5585 5617 7098 7130 5585 Size: 32 1481 32 5971 Fragments arranged by size: 5971 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3455 3455 Size: 7516 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7516 PsiI TTA'TAA Cuts at: 5086 5086 Size: 7516 PspOMI G'GGCC_C Cuts at: 2976 3412 3993 4255 2976 Size: 436 581 262 6237 Fragments arranged by size: 6237 581 436 262 PstI C_TGCA'G Cuts at: 830 2472 3102 3111 4090 4620 4868 830 Size: 1642 630 9 979 530 248 3478 Fragments arranged by size: 3478 1642 979 630 530 248 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7516 PvuII CAG'CTG Cuts at: 2467 3094 4475 2467 Size: 627 1381 5508 Fragments arranged by size: 5508 1381 627 RsrII CG'GwC_CG Cuts at: 2346 2346 Size: 7516 SacI G_AGCT'C Cuts at: 3636 3636 Size: 7516 SacII CC_GC'GG Cuts at: 3052 3052 Size: 7516 SalI G'TCGA_C Cuts at: 1936 3317 4858 1936 Size: 1381 1541 4594 Fragments arranged by size: 4594 1541 1381 SbfI CC_TGCA'GG Cuts at: 3111 4868 3111 Size: 1757 5759 ScaI AGT'ACT Cuts at: 5764 7476 5764 Size: 1712 5804 SfcI C'TryA_G Cuts at: 826 1045 2468 2638 3098 3107 3322 3700 4086 Size: 219 1423 170 460 9 215 378 386 Cuts at: 4086 4213 4554 4616 4864 5999 6680 6871 826 Size: 127 341 62 248 1135 681 191 1471 Fragments arranged by size: 1471 1423 1135 681 460 386 378 341 248 219 215 191 170 127 62 9 SfiI GGCCn_nnn'nGGCC Cuts at: 3254 3254 Size: 7516 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7516 SgrAI Cr'CCGG_yG Cuts at: 1700 4392 1700 Size: 2692 4824 SmaI CCC'GGG Cuts at: 1872 2244 4843 1872 Size: 372 2599 4545 Fragments arranged by size: 4545 2599 372 SmlI C'TyrA_G Cuts at: 820 1017 1943 3398 4109 4382 5620 6521 6783 Size: 197 926 1455 711 273 1238 901 262 Cuts at: 6783 820 Size: 1553 Fragments arranged by size: 1553 1455 1238 926 901 711 273 262 197 SnaBI TAC'GTA Cuts at: 493 4813 5307 493 Size: 4320 494 2702 Fragments arranged by size: 4320 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7516 SphI G_CATG'C Cuts at: 1132 4874 1132 Size: 3742 3774 SspI AAT'ATT Cuts at: 5 52 5440 5 Size: 47 5388 2081 Fragments arranged by size: 5388 2081 47 StuI AGG'CCT Cuts at: 1517 2237 3269 3464 1517 Size: 720 1032 195 5569 Fragments arranged by size: 5569 1032 720 195 StyI C'CwwG_G Cuts at: 513 1065 2022 2958 3165 3274 3388 3408 3975 Size: 552 957 936 207 109 114 20 567 Cuts at: 3975 4623 4955 513 Size: 648 332 3074 Fragments arranged by size: 3074 957 936 648 567 552 332 207 114 109 20 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2363 2792 3135 4706 5543 5560 5713 Size: 1255 783 429 343 1571 837 17 153 Cuts at: 5713 5898 6343 325 Size: 185 445 1498 Fragments arranged by size: 1571 1498 1255 837 783 445 429 343 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3837 5762 7474 Size: 274 80 33 51 1443 1860 1925 1712 Cuts at: 7474 96 Size: 138 Fragments arranged by size: 1925 1860 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2660 2692 7476 7508 2660 Size: 32 4784 32 2668 Fragments arranged by size: 4784 2668 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7516 XbaI T'CTAG_A Cuts at: 4852 4852 Size: 7516 XhoI C'TCGA_G Cuts at: 1943 4109 1943 Size: 2166 5350 XmaI C'CCGG_G Cuts at: 1870 2242 4841 1870 Size: 372 2599 4545 Fragments arranged by size: 4545 2599 372 XmnI GAAnn'nnTTC Cuts at: 2720 5645 2720 Size: 2925 4591 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3724 5324 276 Size: 53 83 186 936 2190 1600 2468 Fragments arranged by size: 2468 2190 1600 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NruI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BfrBI BsiWI Bsu36I EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NsiI PacI PfoI PmlI PspXI PsrI SanDI SapI SexAI SfoI SrfI SwaI XcmI