(Circular) (Six-base) MAPSORT of: pFN21ASDA0119.seq Check: 8991 from: 1 to: 5698 pFN21ASDA0119 5698 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3508 278 Size: 53 83 186 936 1972 2468 Fragments arranged by size: 2468 1972 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3041 1937 Size: 1104 4594 Acc65I G'GTAC_C Cuts at: 3019 3019 Size: 5698 AclI AA'CG_TT Cuts at: 1563 3825 4198 1563 Size: 2262 373 3063 Fragments arranged by size: 3063 2262 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3761 5145 5413 1632 Size: 2129 1384 268 1917 Fragments arranged by size: 2129 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5501 AflIII A'CryG_T Cuts at: 1203 4597 5574 1203 Size: 3394 977 1327 Fragments arranged by size: 3394 1327 977 AgeI A'CCGG_T Cuts at: 4529 4529 Size: 5698 AhdI GACnn_n'nnGTC Cuts at: 4427 4427 Size: 5698 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5698 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5698 AlwNI CAG_nnn'CTG Cuts at: 3441 5013 3441 Size: 1572 4126 ApaLI G'TGCA_C Cuts at: 3755 4911 3755 Size: 1156 4542 ApoI r'AATT_y Cuts at: 1493 3007 3233 3385 5494 1493 Size: 1514 226 152 2109 1697 Fragments arranged by size: 2109 1697 1514 226 152 AseI AT'TA_AT Cuts at: 160 1032 4252 160 Size: 872 3220 1606 Fragments arranged by size: 3220 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5698 AvaI C'yCGr_G Cuts at: 1870 1943 3023 1870 Size: 73 1080 4545 Fragments arranged by size: 4545 1080 73 BamHI G'GATC_C Cuts at: 3028 3395 3028 Size: 367 5331 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2087 3019 4474 618 Size: 325 217 627 300 932 1455 1842 Fragments arranged by size: 1842 1455 932 627 325 300 217 BanII G_rGCy'C Cuts at: 1391 3017 1391 Size: 1626 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2259 928 Size: 971 360 4367 Fragments arranged by size: 4367 971 360 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3889 3923 1903 Size: 34 1952 34 3678 Fragments arranged by size: 3678 1952 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3590 4800 3590 Size: 1210 4488 BclI T'GATC_A Cuts at: 1548 1548 Size: 5698 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4309 136 Size: 107 122 71 631 605 2637 1525 Fragments arranged by size: 2637 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5693 5693 Size: 5698 BlpI GC'TnA_GC Cuts at: 3115 3115 Size: 5698 Bme1580I G_kGCm'C Cuts at: 1790 3759 4915 1790 Size: 1969 1156 2573 Fragments arranged by size: 2573 1969 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5698 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2732 3429 4387 Size: 416 53 83 399 24 1315 697 958 Cuts at: 4387 442 Size: 1753 Fragments arranged by size: 1753 1315 958 697 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2961 1056 Size: 1905 3793 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2085 2561 4358 1239 Size: 846 476 1797 2579 Fragments arranged by size: 2579 1797 846 476 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2552 3823 4688 4986 2552 Size: 1271 865 298 3264 Fragments arranged by size: 3264 1271 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4361 882 Size: 617 2862 2219 Fragments arranged by size: 2862 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2995 3489 493 Size: 711 1791 494 2702 Fragments arranged by size: 2702 1791 711 494 BsaBI GATnn'nnATC Cuts at: 5692 5692 Size: 5698 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3505 3887 4522 275 Size: 53 83 186 936 1972 382 635 1451 Fragments arranged by size: 1972 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2616 4131 4529 4803 4950 5361 2616 Size: 1515 398 274 147 411 2953 Fragments arranged by size: 2953 1515 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2699 2729 1178 Size: 30 165 30 1296 30 4147 Fragments arranged by size: 4147 1296 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2210 1186 Size: 1024 4674 BseYI C'CCAG_C Cuts at: 2153 2776 4901 5397 2153 Size: 623 2125 496 2454 Fragments arranged by size: 2454 2125 623 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2885 1163 Size: 573 1149 3976 Fragments arranged by size: 3976 1149 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3148 3909 4937 1540 Size: 275 186 1147 761 1028 2301 Fragments arranged by size: 2301 1147 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2677 2727 3759 3844 4915 2677 Size: 50 1032 85 1071 3460 Fragments arranged by size: 3460 1071 1032 85 50 BsmI GAATG_Cn' Cuts at: 3209 3302 3209 Size: 93 5605 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2677 2727 3017 3759 3844 4915 1391 Size: 399 887 50 290 742 85 1071 2174 Fragments arranged by size: 2174 1071 887 742 399 290 85 50 BspEI T'CCGG_A Cuts at: 5361 5361 Size: 5698 BspHI T'CATG_A Cuts at: 3587 5317 3587 Size: 1730 3968 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2191 2857 3053 844 Size: 1347 666 196 3489 Fragments arranged by size: 3489 1347 666 196 BsrBI CCG'CTC Cuts at: 1394 1823 3585 1394 Size: 429 1762 3507 Fragments arranged by size: 3507 1762 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4193 4367 65 Size: 1183 2945 174 1396 Fragments arranged by size: 2945 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4342 4529 1700 Size: 110 2532 187 2869 Fragments arranged by size: 2869 2532 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5698 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5698 BssSI C'ACGA_G Cuts at: 3758 4770 3758 Size: 1012 4686 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5698 BstBI TT'CG_AA Cuts at: 2007 4502 2007 Size: 2495 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5698 BstYI r'GATC_y Cuts at: 1972 2449 2681 3028 3395 3780 3797 5249 5335 Size: 477 232 347 367 385 17 1452 86 Cuts at: 5335 5347 5693 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 477 385 367 347 346 232 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2472 513 Size: 552 957 450 3739 Fragments arranged by size: 3739 957 552 450 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4959 Fragments arranged by size: 4959 683 56 BtsI GCAGTG_nn' Cuts at: 2045 2245 3214 4008 4028 2045 Size: 200 969 794 20 3715 Fragments arranged by size: 3715 969 794 200 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5698 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5663 DraI TTT'AAA Cuts at: 3002 3002 Size: 5698 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4705 809 Size: 507 3389 1802 Fragments arranged by size: 3389 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2059 2211 2499 Size: 54 1425 50 275 18 229 152 288 Cuts at: 2499 2826 3145 4034 5663 8 Size: 327 319 889 1629 43 Fragments arranged by size: 1629 1425 889 327 319 288 275 229 152 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3145 1537 Size: 275 1333 4090 Fragments arranged by size: 4090 1333 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2240 3628 1353 Size: 887 1388 3423 Fragments arranged by size: 3423 1388 887 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2150 4285 4659 4805 387 Size: 1064 138 561 2135 374 146 1280 Fragments arranged by size: 2135 1280 1064 561 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2085 2561 3761 4358 5145 5413 1239 Size: 393 453 476 1200 597 787 268 1524 Fragments arranged by size: 1524 1200 787 597 476 453 393 268 EcoO109I rG'GnC_Cy Cuts at: 2734 2734 Size: 5698 EcoRI G'AATT_C Cuts at: 3007 3385 3007 Size: 378 5320 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2139 2139 Size: 5698 FspI TGC'GCA Cuts at: 4204 4204 Size: 5698 HaeII r_GCGC'y Cuts at: 1678 4845 1678 Size: 3167 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2199 2231 2699 2731 4339 Size: 32 135 32 627 32 468 32 1608 Cuts at: 4339 4371 4413 4445 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1608 627 468 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2356 2886 3042 3288 669 Size: 988 281 418 530 156 246 3079 Fragments arranged by size: 3079 988 530 418 281 246 156 HindIII A'AGCT_T Cuts at: 748 2121 2273 748 Size: 1373 152 4173 Fragments arranged by size: 4173 1373 152 HpaI GTT'AAC Cuts at: 3288 3288 Size: 5698 KpnI G_GTAC'C Cuts at: 3023 3023 Size: 5698 MfeI C'AATT_G Cuts at: 2342 3297 2342 Size: 955 4743 MluI A'CGCG_T Cuts at: 5574 5574 Size: 5698 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2185 2332 4812 4996 1305 Size: 880 147 2480 184 2007 Fragments arranged by size: 2480 2007 880 184 147 MscI TGG'CCA Cuts at: 10 64 1489 1832 2061 2213 2501 2828 10 Size: 54 1425 343 229 152 288 327 2880 Fragments arranged by size: 2880 1425 343 327 288 229 152 54 MslI CAynn'nnrTG Cuts at: 518 1166 2513 3656 4015 4174 518 Size: 648 1347 1143 359 159 2042 Fragments arranged by size: 2042 1347 1143 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2539 2803 3114 3791 4939 5184 1606 Size: 933 264 311 677 1148 245 2120 Fragments arranged by size: 2120 1148 933 677 311 264 245 NcoI C'CATG_G Cuts at: 513 1065 2022 2472 513 Size: 552 957 450 3739 Fragments arranged by size: 3739 957 552 450 NdeI CA'TA_TG Cuts at: 387 387 Size: 5698 NheI G'CTAG_C Cuts at: 1052 2957 1052 Size: 1905 3793 NotI GC'GGCC_GC Cuts at: 3145 3145 Size: 5698 NspI r_CATG'y Cuts at: 1132 1229 3056 4601 1132 Size: 97 1827 1545 2229 Fragments arranged by size: 2229 1827 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5698 PciI A'CATG_T Cuts at: 4597 4597 Size: 5698 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5698 PmeI GTTT'AAAC Cuts at: 3002 3002 Size: 5698 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3767 3799 5280 5312 3767 Size: 32 1481 32 4153 Fragments arranged by size: 4153 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2734 2734 Size: 5698 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5698 PsiI TTA'TAA Cuts at: 3268 3268 Size: 5698 PstI C_TGCA'G Cuts at: 830 2808 2893 3050 830 Size: 1978 85 157 3478 Fragments arranged by size: 3478 1978 157 85 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5698 PvuII CAG'CTG Cuts at: 2539 2803 2539 Size: 264 5434 SalI G'TCGA_C Cuts at: 1936 3040 1936 Size: 1104 4594 SbfI CC_TGCA'GG Cuts at: 3050 3050 Size: 5698 ScaI AGT'ACT Cuts at: 3946 5658 3946 Size: 1712 3986 SexAI A'CCwGG_T Cuts at: 2589 2589 Size: 5698 SfcI C'TryA_G Cuts at: 826 1045 2668 2804 2889 3046 4181 4862 5053 Size: 219 1623 136 85 157 1135 681 191 Cuts at: 5053 826 Size: 1471 Fragments arranged by size: 1623 1471 1135 681 219 191 157 136 85 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5698 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5698 SmaI CCC'GGG Cuts at: 1872 3025 1872 Size: 1153 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2567 3802 4703 4965 820 Size: 197 926 624 1235 901 262 1553 Fragments arranged by size: 1553 1235 926 901 624 262 197 SnaBI TAC'GTA Cuts at: 493 2995 3489 493 Size: 2502 494 2702 Fragments arranged by size: 2702 2502 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5698 SphI G_CATG'C Cuts at: 1132 3056 1132 Size: 1924 3774 SspI AAT'ATT Cuts at: 5 52 2436 2990 3622 5 Size: 47 2384 554 632 2081 Fragments arranged by size: 2384 2081 632 554 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5698 StyI C'CwwG_G Cuts at: 513 1065 2022 2316 2472 2751 3137 513 Size: 552 957 294 156 279 386 3074 Fragments arranged by size: 3074 957 552 386 294 279 156 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2692 3725 3742 3895 4080 4525 325 Size: 1255 1112 1033 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1112 1033 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2571 3944 5656 Size: 274 80 33 51 1443 594 1373 1712 Cuts at: 5656 96 Size: 138 Fragments arranged by size: 1712 1443 1373 594 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5658 5690 5658 Size: 32 5666 Tth111I GACn'n_nGTC Cuts at: 1316 2738 1316 Size: 1422 4276 XbaI T'CTAG_A Cuts at: 3034 3034 Size: 5698 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2807 2807 Size: 5698 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5698 XmaI C'CCGG_G Cuts at: 1870 3023 1870 Size: 1153 4545 XmnI GAAnn'nnTTC Cuts at: 3827 3827 Size: 5698 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3506 276 Size: 53 83 186 936 1972 2468 Fragments arranged by size: 2468 1972 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SacII SanDI SapI SfiI SfoI SrfI SwaI