(Circular) (Six-base) MAPSORT of: pFN21ASDA0115.seq Check: 2354 from: 1 to: 6097 pFN21ASDA0115 6097 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2033 2033 Size: 6097 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3907 278 Size: 53 83 186 936 2371 2468 Fragments arranged by size: 2468 2371 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3440 1937 Size: 1503 4594 Acc65I G'GTAC_C Cuts at: 3418 3418 Size: 6097 AclI AA'CG_TT Cuts at: 1563 4224 4597 1563 Size: 2661 373 3063 Fragments arranged by size: 3063 2661 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2243 2585 2776 2797 3146 3331 4160 5544 Size: 611 342 191 21 349 185 829 1384 Cuts at: 5544 5812 1632 Size: 268 1917 Fragments arranged by size: 1917 1384 829 611 349 342 268 191 185 21 AfeI AGC'GCT Cuts at: 3281 3281 Size: 6097 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5900 AflIII A'CryG_T Cuts at: 1203 3171 4996 5973 1203 Size: 1968 1825 977 1327 Fragments arranged by size: 1968 1825 1327 977 AgeI A'CCGG_T Cuts at: 4928 4928 Size: 6097 AhdI GACnn_n'nnGTC Cuts at: 4826 4826 Size: 6097 AleI CACnn'nnGTG Cuts at: 1166 2165 1166 Size: 999 5098 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2183 1184 Size: 999 5098 AlwNI CAG_nnn'CTG Cuts at: 2048 3840 5412 2048 Size: 1792 1572 2733 Fragments arranged by size: 2733 1792 1572 ApaI G_GGCC'C Cuts at: 2107 2107 Size: 6097 ApaLI G'TGCA_C Cuts at: 4154 5310 4154 Size: 1156 4941 ApoI r'AATT_y Cuts at: 1493 2294 3406 3632 3784 5893 1493 Size: 801 1112 226 152 2109 1697 Fragments arranged by size: 2109 1697 1112 801 226 152 AseI AT'TA_AT Cuts at: 160 1032 4651 160 Size: 872 3619 1606 Fragments arranged by size: 3619 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6097 AvaI C'yCGr_G Cuts at: 1870 1943 2094 3422 1870 Size: 73 151 1328 4545 Fragments arranged by size: 4545 1328 151 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 3228 3261 3228 Size: 33 6064 BamHI G'GATC_C Cuts at: 3427 3794 3427 Size: 367 5730 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2851 3418 4873 618 Size: 325 217 627 1064 567 1455 1842 Fragments arranged by size: 1842 1455 1064 627 567 325 217 BanII G_rGCy'C Cuts at: 1391 2082 2107 2244 2675 2763 3339 3416 1391 Size: 691 25 137 431 88 576 77 4072 Fragments arranged by size: 4072 691 576 431 137 88 77 25 BbeI G_GCGC'C Cuts at: 2855 2855 Size: 6097 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3349 928 Size: 971 1450 3676 Fragments arranged by size: 3676 1450 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4288 4322 1903 Size: 34 2351 34 3678 Fragments arranged by size: 3678 2351 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2730 3256 3989 5199 2730 Size: 526 733 1210 3628 Fragments arranged by size: 3628 1210 733 526 BclI T'GATC_A Cuts at: 1548 1548 Size: 6097 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4708 136 Size: 107 122 71 631 605 3036 1525 Fragments arranged by size: 3036 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6092 6092 Size: 6097 BlpI GC'TnA_GC Cuts at: 3514 3514 Size: 6097 Bme1580I G_kGCm'C Cuts at: 1790 2107 4158 5314 1790 Size: 317 2051 1156 2573 Fragments arranged by size: 2573 2051 1156 317 BmgBI CAC'GTC Cuts at: 1318 3172 1318 Size: 1854 4243 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2710 3828 4786 Size: 416 53 83 399 24 1293 1118 958 Cuts at: 4786 442 Size: 1753 Fragments arranged by size: 1753 1293 1118 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6097 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2747 3107 3245 4757 1239 Size: 1508 360 138 1512 2579 Fragments arranged by size: 2579 1512 1508 360 138 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4222 5087 5385 4222 Size: 865 298 4934 Fragments arranged by size: 4934 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2357 4760 882 Size: 617 858 2403 2219 Fragments arranged by size: 2403 2219 858 617 BsaAI yAC'GTr Cuts at: 493 1204 3394 3888 493 Size: 711 2190 494 2702 Fragments arranged by size: 2702 2190 711 494 BsaBI GATnn'nnATC Cuts at: 6091 6091 Size: 6097 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2852 3904 4286 4921 Size: 53 83 186 936 1319 1052 382 635 Cuts at: 4921 275 Size: 1451 Fragments arranged by size: 1451 1319 1052 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2034 2214 4530 4928 5202 5349 5760 2034 Size: 180 2316 398 274 147 411 2371 Fragments arranged by size: 2371 2316 411 398 274 180 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2382 2412 1178 Size: 30 165 30 979 30 4863 Fragments arranged by size: 4863 979 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2080 2489 2738 2942 1186 Size: 894 409 249 204 4341 Fragments arranged by size: 4341 894 409 249 204 BseYI C'CCAG_C Cuts at: 2048 2081 2267 2443 2756 2913 3331 5300 5796 Size: 33 186 176 313 157 418 1969 496 Cuts at: 5796 2048 Size: 2349 Fragments arranged by size: 2349 1969 496 418 313 186 176 157 33 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2061 2861 1163 Size: 573 325 800 4399 Fragments arranged by size: 4399 800 573 325 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3547 4308 5336 1540 Size: 275 186 1546 761 1028 2301 Fragments arranged by size: 2301 1546 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2244 4158 4243 5314 2244 Size: 1914 85 1071 3027 Fragments arranged by size: 3027 1914 1071 85 BsmI GAATG_Cn' Cuts at: 3608 3701 3608 Size: 93 6004 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2082 2107 2244 2675 2763 3339 3416 Size: 399 292 25 137 431 88 576 77 Cuts at: 3416 4158 4243 5314 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 576 431 399 292 137 88 85 77 25 BspEI T'CCGG_A Cuts at: 2214 5760 2214 Size: 3546 2551 BspHI T'CATG_A Cuts at: 3986 5716 3986 Size: 1730 4367 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2033 2568 3452 844 Size: 1189 535 884 3489 Fragments arranged by size: 3489 1189 884 535 BsrBI CCG'CTC Cuts at: 1394 1823 3984 1394 Size: 429 2161 3507 Fragments arranged by size: 3507 2161 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2751 4592 4766 65 Size: 1183 1503 1841 174 1396 Fragments arranged by size: 1841 1503 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3207 4741 4928 1700 Size: 110 1397 1534 187 2869 Fragments arranged by size: 2869 1534 1397 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6097 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6097 BssSI C'ACGA_G Cuts at: 4157 5169 4157 Size: 1012 5085 BstAPI GCAn_nnn'nTGC Cuts at: 794 2454 794 Size: 1660 4437 BstBI TT'CG_AA Cuts at: 2007 4901 2007 Size: 2894 3203 BstEII G'GTnAC_C Cuts at: 1184 2426 1184 Size: 1242 4855 BstXI CCAn_nnnn'nTGG Cuts at: 3331 3331 Size: 6097 BstYI r'GATC_y Cuts at: 1972 3427 3794 4179 4196 5648 5734 5746 6092 Size: 1455 367 385 17 1452 86 12 346 Cuts at: 6092 1972 Size: 1977 Fragments arranged by size: 1977 1455 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2022 2088 513 Size: 552 957 66 4522 Fragments arranged by size: 4522 957 552 66 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3087 487 Size: 683 56 1861 3497 Fragments arranged by size: 3497 1861 683 56 BtsI GCAGTG_nn' Cuts at: 2401 2455 2844 3613 4407 4427 2401 Size: 54 389 769 794 20 4071 Fragments arranged by size: 4071 794 769 389 54 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6097 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6062 DraI TTT'AAA Cuts at: 3193 3401 3193 Size: 208 5889 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5104 809 Size: 507 3788 1802 Fragments arranged by size: 3788 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2524 2631 3254 Size: 54 1425 50 275 18 694 107 623 Cuts at: 3254 3544 4433 6062 8 Size: 290 889 1629 43 Fragments arranged by size: 1629 1425 889 694 623 290 275 107 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3544 1537 Size: 275 1732 4090 Fragments arranged by size: 4090 1732 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2439 2680 3238 3344 4027 1353 Size: 1086 241 558 106 683 3423 Fragments arranged by size: 3423 1086 683 558 241 106 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2403 4684 5058 5204 387 Size: 1064 138 814 2281 374 146 1280 Fragments arranged by size: 2281 1280 1064 814 374 146 138 EcoICRI GAG'CTC Cuts at: 2242 2242 Size: 6097 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2243 2585 2747 2776 2797 3107 3146 Size: 393 611 342 162 29 21 310 39 Cuts at: 3146 3245 3331 4160 4757 5544 5812 1239 Size: 99 86 829 597 787 268 1524 Fragments arranged by size: 1524 829 787 611 597 393 342 310 268 162 99 86 39 29 21 EcoNI CCTnn'n_nnAGG Cuts at: 2059 2564 2059 Size: 505 5592 EcoO109I rG'GnC_Cy Cuts at: 2052 2104 2569 2948 3058 3117 2052 Size: 52 465 379 110 59 5032 Fragments arranged by size: 5032 465 379 110 59 52 EcoRI G'AATT_C Cuts at: 2294 3406 3784 2294 Size: 1112 378 4607 Fragments arranged by size: 4607 1112 378 FspI TGC'GCA Cuts at: 4603 4603 Size: 6097 HaeII r_GCGC'y Cuts at: 1678 2855 3283 3318 5244 1678 Size: 1177 428 35 1926 2531 Fragments arranged by size: 2531 1926 1177 428 35 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2337 2369 4738 4770 4812 Size: 32 135 32 765 32 2369 32 42 Cuts at: 4812 4844 1373 Size: 32 2626 Fragments arranged by size: 2626 2369 765 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3441 3687 669 Size: 988 281 1503 246 3079 Fragments arranged by size: 3079 1503 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6097 HpaI GTT'AAC Cuts at: 3687 3687 Size: 6097 KasI G'GCGC_C Cuts at: 2851 2851 Size: 6097 KpnI G_GTAC'C Cuts at: 3422 3422 Size: 6097 MfeI C'AATT_G Cuts at: 3696 3696 Size: 6097 MluI A'CGCG_T Cuts at: 5973 5973 Size: 6097 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2442 3411 5211 5395 1305 Size: 1137 969 1800 184 2007 Fragments arranged by size: 2007 1800 1137 969 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2526 10 Size: 54 1425 343 694 3581 Fragments arranged by size: 3581 1425 694 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2165 2357 2505 4055 4414 4573 518 Size: 648 999 192 148 1550 359 159 2042 Fragments arranged by size: 2042 1550 999 648 359 192 159 148 MspA1I CmG'CkG Cuts at: 1606 2090 2151 2258 2796 2913 3085 3513 4190 Size: 484 61 107 538 117 172 428 677 Cuts at: 4190 5338 5583 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 538 484 428 245 172 117 107 61 NarI GG'CG_CC Cuts at: 2852 2852 Size: 6097 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4588 Fragments arranged by size: 4588 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6097 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6097 NotI GC'GGCC_GC Cuts at: 3544 3544 Size: 6097 NspI r_CATG'y Cuts at: 1132 1229 2729 3455 5000 1132 Size: 97 1500 726 1545 2229 Fragments arranged by size: 2229 1545 1500 726 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6097 PciI A'CATG_T Cuts at: 4996 4996 Size: 6097 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6097 PfoI T'CCnGG_A Cuts at: 2697 2697 Size: 6097 PmeI GTTT'AAAC Cuts at: 3401 3401 Size: 6097 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4166 4198 5679 5711 4166 Size: 32 1481 32 4552 Fragments arranged by size: 4552 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2052 3058 3117 2052 Size: 1006 59 5032 Fragments arranged by size: 5032 1006 59 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6097 PsiI TTA'TAA Cuts at: 3667 3667 Size: 6097 PspOMI G'GGCC_C Cuts at: 2103 2103 Size: 6097 PstI C_TGCA'G Cuts at: 830 3449 830 Size: 2619 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6097 PvuII CAG'CTG Cuts at: 3085 3085 Size: 6097 SacI G_AGCT'C Cuts at: 2244 2244 Size: 6097 SacII CC_GC'GG Cuts at: 2091 2091 Size: 6097 SalI G'TCGA_C Cuts at: 1936 3439 1936 Size: 1503 4594 SanDI GG'GwC_CC Cuts at: 3058 3058 Size: 6097 SapI GCTCTTCn'nnn_ Cuts at: 2680 3344 2680 Size: 664 5433 SbfI CC_TGCA'GG Cuts at: 3449 3449 Size: 6097 ScaI AGT'ACT Cuts at: 4345 6057 4345 Size: 1712 4385 SfcI C'TryA_G Cuts at: 826 1045 3445 4580 5261 5452 826 Size: 219 2400 1135 681 191 1471 Fragments arranged by size: 2400 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2853 2853 Size: 6097 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6097 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6097 SmaI CCC'GGG Cuts at: 1872 2096 3424 1872 Size: 224 1328 4545 Fragments arranged by size: 4545 1328 224 SmlI C'TyrA_G Cuts at: 820 1017 1943 4201 5102 5364 820 Size: 197 926 2258 901 262 1553 Fragments arranged by size: 2258 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3394 3888 493 Size: 2901 494 2702 Fragments arranged by size: 2901 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6097 SphI G_CATG'C Cuts at: 1132 3455 1132 Size: 2323 3774 SrfI GCCC'GGGC Cuts at: 2096 2096 Size: 6097 SspI AAT'ATT Cuts at: 5 52 4021 5 Size: 47 3969 2081 Fragments arranged by size: 3969 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6097 StyI C'CwwG_G Cuts at: 513 1065 2022 2521 3536 513 Size: 552 957 499 1015 3074 Fragments arranged by size: 3074 1015 957 552 499 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2729 2905 3001 4124 4141 4294 4479 Size: 1255 1149 176 96 1123 17 153 185 Cuts at: 4479 4924 325 Size: 445 1498 Fragments arranged by size: 1498 1255 1149 1123 445 185 176 153 96 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3228 4343 6055 Size: 274 80 33 51 1443 1251 1115 1712 Cuts at: 6055 96 Size: 138 Fragments arranged by size: 1712 1443 1251 1115 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2382 2414 2704 2736 6057 6089 2382 Size: 32 290 32 3321 32 2390 Fragments arranged by size: 3321 2390 290 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6097 XbaI T'CTAG_A Cuts at: 3433 3433 Size: 6097 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6097 XmaI C'CCGG_G Cuts at: 1870 2094 3422 1870 Size: 224 1328 4545 Fragments arranged by size: 4545 1328 224 XmnI GAAnn'nnTTC Cuts at: 4226 4226 Size: 6097 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3905 276 Size: 53 83 186 936 2371 2468 Fragments arranged by size: 2468 2371 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SexAI SfiI SwaI XcmI