(Circular) (Six-base) MAPSORT of: pFN21ASDA0111.seq Check: 5342 from: 1 to: 5962 pFN21ASDA0111 5962 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3772 278 Size: 53 83 186 936 2236 2468 Fragments arranged by size: 2468 2236 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2634 3305 1937 Size: 697 671 4594 Fragments arranged by size: 4594 697 671 Acc65I G'GTAC_C Cuts at: 2640 3283 2640 Size: 643 5319 AclI AA'CG_TT Cuts at: 1563 3240 4089 4462 1563 Size: 1677 849 373 3063 Fragments arranged by size: 3063 1677 849 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2281 4025 5409 5677 1632 Size: 649 1744 1384 268 1917 Fragments arranged by size: 1917 1744 1384 649 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5765 AflIII A'CryG_T Cuts at: 1203 2555 4861 5838 1203 Size: 1352 2306 977 1327 Fragments arranged by size: 2306 1352 1327 977 AgeI A'CCGG_T Cuts at: 4793 4793 Size: 5962 AhdI GACnn_n'nnGTC Cuts at: 4691 4691 Size: 5962 AleI CACnn'nnGTG Cuts at: 1166 2660 1166 Size: 1494 4468 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5962 AlwNI CAG_nnn'CTG Cuts at: 3705 5277 3705 Size: 1572 4390 ApaLI G'TGCA_C Cuts at: 4019 5175 4019 Size: 1156 4806 ApoI r'AATT_y Cuts at: 1493 2102 2813 3271 3497 3649 5758 1493 Size: 609 711 458 226 152 2109 1697 Fragments arranged by size: 2109 1697 711 609 458 226 152 AseI AT'TA_AT Cuts at: 160 1032 4516 160 Size: 872 3484 1606 Fragments arranged by size: 3484 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 AvaI C'yCGr_G Cuts at: 1870 1943 2050 3287 1870 Size: 73 107 1237 4545 Fragments arranged by size: 4545 1237 107 73 BamHI G'GATC_C Cuts at: 3292 3659 3292 Size: 367 5595 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2450 2640 2999 3283 4738 Size: 325 217 627 663 190 359 284 1455 Cuts at: 4738 618 Size: 1842 Fragments arranged by size: 1842 1455 663 627 359 325 284 217 190 BanII G_rGCy'C Cuts at: 1391 2057 3281 1391 Size: 666 1224 4072 Fragments arranged by size: 4072 1224 666 BbeI G_GCGC'C Cuts at: 3003 3003 Size: 5962 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2091 928 Size: 971 192 4799 Fragments arranged by size: 4799 971 192 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4153 4187 1903 Size: 34 2216 34 3678 Fragments arranged by size: 3678 2216 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2944 3854 5064 2944 Size: 910 1210 3842 Fragments arranged by size: 3842 1210 910 BclI T'GATC_A Cuts at: 1548 1548 Size: 5962 BfrBI ATG'CAT Cuts at: 2944 2944 Size: 5962 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2042 4573 136 Size: 107 122 71 631 605 370 2531 1525 Fragments arranged by size: 2531 1525 631 605 370 122 107 71 BglII A'GATC_T Cuts at: 5957 5957 Size: 5962 BlpI GC'TnA_GC Cuts at: 3379 3379 Size: 5962 Bme1580I G_kGCm'C Cuts at: 1790 2510 4023 5179 1790 Size: 720 1513 1156 2573 Fragments arranged by size: 2573 1513 1156 720 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5962 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3693 4651 442 Size: 416 53 83 399 24 2276 958 1753 Fragments arranged by size: 2276 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5962 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2297 2496 2634 2720 4622 1239 Size: 1058 199 138 86 1902 2579 Fragments arranged by size: 2579 1902 1058 199 138 86 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2328 4087 4952 5250 2328 Size: 1759 865 298 3040 Fragments arranged by size: 3040 1759 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2102 4625 882 Size: 617 603 2523 2219 Fragments arranged by size: 2523 2219 617 603 BsaAI yAC'GTr Cuts at: 493 1204 2558 3259 3753 493 Size: 711 1354 701 494 2702 Fragments arranged by size: 2702 1354 711 701 494 BsaBI GATnn'nnATC Cuts at: 5956 5956 Size: 5962 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3000 3769 4151 4786 Size: 53 83 186 936 1467 769 382 635 Cuts at: 4786 275 Size: 1451 Fragments arranged by size: 1467 1451 936 769 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2991 4395 4793 5067 5214 5625 2991 Size: 1404 398 274 147 411 3328 Fragments arranged by size: 3328 1404 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2978 3008 3112 3142 1178 Size: 30 165 30 1575 30 104 30 3998 Fragments arranged by size: 3998 1575 165 104 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2132 1186 Size: 946 5016 BseYI C'CCAG_C Cuts at: 5165 5661 5165 Size: 496 5466 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2402 1163 Size: 573 666 4723 Fragments arranged by size: 4723 666 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2996 3139 3412 4173 5201 1540 Size: 275 186 995 143 273 761 1028 2301 Fragments arranged by size: 2301 1028 995 761 275 273 186 143 BsiHKAI G_wGCw'C Cuts at: 4023 4108 5179 4023 Size: 85 1071 4806 Fragments arranged by size: 4806 1071 85 BsmI GAATG_Cn' Cuts at: 3473 3566 3473 Size: 93 5869 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2057 2510 3281 4023 4108 5179 1391 Size: 399 267 453 771 742 85 1071 2174 Fragments arranged by size: 2174 1071 771 742 453 399 267 85 BspEI T'CCGG_A Cuts at: 5625 5625 Size: 5962 BspHI T'CATG_A Cuts at: 2718 2979 3851 5581 2718 Size: 261 872 1730 3099 Fragments arranged by size: 3099 1730 872 261 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2175 2652 3317 844 Size: 1331 477 665 3489 Fragments arranged by size: 3489 1331 665 477 BsrBI CCG'CTC Cuts at: 1394 1823 2968 3849 1394 Size: 429 1145 881 3507 Fragments arranged by size: 3507 1145 881 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4457 4631 65 Size: 1183 3209 174 1396 Fragments arranged by size: 3209 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4606 4793 1700 Size: 110 2796 187 2869 Fragments arranged by size: 2869 2796 187 110 BsrGI T'GTAC_A Cuts at: 96 3102 96 Size: 3006 2956 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5962 BssSI C'ACGA_G Cuts at: 2690 4022 5034 2690 Size: 1332 1012 3618 Fragments arranged by size: 3618 1332 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5962 BstBI TT'CG_AA Cuts at: 2007 4766 2007 Size: 2759 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5962 BstXI CCAn_nnnn'nTGG Cuts at: 2662 3043 2662 Size: 381 5581 BstYI r'GATC_y Cuts at: 1972 2385 3292 3659 4044 4061 5513 5599 5611 Size: 413 907 367 385 17 1452 86 12 Cuts at: 5611 5957 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 907 413 385 367 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2635 2635 Size: 5962 Bsu36I CC'TnA_GG Cuts at: 2549 3054 2549 Size: 505 5457 BtgI C'CryG_G Cuts at: 513 1065 2022 2031 2148 513 Size: 552 957 9 117 4327 Fragments arranged by size: 4327 957 552 117 9 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2242 487 Size: 683 56 1016 4207 Fragments arranged by size: 4207 1016 683 56 BtsI GCAGTG_nn' Cuts at: 2799 3478 4272 4292 2799 Size: 679 794 20 4469 Fragments arranged by size: 4469 794 679 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5962 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 3202 3237 529 Size: 35 2638 35 3254 Fragments arranged by size: 3254 2638 35 35 DraI TTT'AAA Cuts at: 3266 3266 Size: 5962 DraIII CAC_nnn'GTG Cuts at: 2826 2826 Size: 5962 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4969 809 Size: 507 3653 1802 Fragments arranged by size: 3653 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2034 3136 3150 Size: 54 1425 50 275 18 204 1102 14 Cuts at: 3150 3409 4298 5927 8 Size: 259 889 1629 43 Fragments arranged by size: 1629 1425 1102 889 275 259 204 54 50 43 18 14 EagI C'GGCC_G Cuts at: 1537 1812 3136 3409 1537 Size: 275 1324 273 4090 Fragments arranged by size: 4090 1324 275 273 EarI CTCTTCn'nnn_ Cuts at: 1353 2799 3892 1353 Size: 1446 1093 3423 Fragments arranged by size: 3423 1446 1093 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4549 4923 5069 387 Size: 1064 138 2960 374 146 1280 Fragments arranged by size: 2960 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2281 2297 2496 2634 2720 4025 4622 Size: 393 649 16 199 138 86 1305 597 Cuts at: 4622 5409 5677 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1305 787 649 597 393 268 199 138 86 16 EcoNI CCTnn'n_nnAGG Cuts at: 2159 2159 Size: 5962 EcoO109I rG'GnC_Cy Cuts at: 2164 2164 Size: 5962 EcoRI G'AATT_C Cuts at: 2102 3271 3649 2102 Size: 1169 378 4415 Fragments arranged by size: 4415 1169 378 EcoRV GAT'ATC Cuts at: 3196 3196 Size: 5962 FspI TGC'GCA Cuts at: 4468 4468 Size: 5962 HaeII r_GCGC'y Cuts at: 1678 3003 5109 1678 Size: 1325 2106 2531 Fragments arranged by size: 2531 2106 1325 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2241 2273 2344 2376 2839 Size: 32 135 32 669 32 71 32 463 Cuts at: 2839 2871 2957 2978 2989 3010 3112 3144 4603 Size: 32 86 21 11 21 102 32 1459 Cuts at: 4603 4635 4677 4709 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1459 669 463 135 102 86 71 42 32 32 32 32 32 32 32 32 21 21 11 HincII GTy'rAC Cuts at: 669 1657 1938 3306 3552 669 Size: 988 281 1368 246 3079 Fragments arranged by size: 3079 1368 988 281 246 HindIII A'AGCT_T Cuts at: 748 2346 748 Size: 1598 4364 HpaI GTT'AAC Cuts at: 3552 3552 Size: 5962 KasI G'GCGC_C Cuts at: 2999 2999 Size: 5962 KpnI G_GTAC'C Cuts at: 2644 3287 2644 Size: 643 5319 MfeI C'AATT_G Cuts at: 3561 3561 Size: 5962 MluI A'CGCG_T Cuts at: 5838 5838 Size: 5962 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3024 5076 5260 1305 Size: 1719 2052 184 2007 Fragments arranged by size: 2052 2007 1719 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3152 10 Size: 54 1425 343 1320 2820 Fragments arranged by size: 2820 1425 1320 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2660 2741 3920 4279 4438 518 Size: 648 1494 81 1179 359 159 2042 Fragments arranged by size: 2042 1494 1179 648 359 159 81 MspA1I CmG'CkG Cuts at: 1606 3378 4055 5203 5448 1606 Size: 1772 677 1148 245 2120 Fragments arranged by size: 2120 1772 1148 677 245 NarI GG'CG_CC Cuts at: 3000 3000 Size: 5962 NcoI C'CATG_G Cuts at: 513 1065 2022 2148 513 Size: 552 957 126 4327 Fragments arranged by size: 4327 957 552 126 NdeI CA'TA_TG Cuts at: 387 387 Size: 5962 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5962 NotI GC'GGCC_GC Cuts at: 3409 3409 Size: 5962 NsiI A_TGCA'T Cuts at: 2946 2946 Size: 5962 NspI r_CATG'y Cuts at: 1132 1229 2498 2956 3320 4865 1132 Size: 97 1269 458 364 1545 2229 Fragments arranged by size: 2229 1545 1269 458 364 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5962 PciI A'CATG_T Cuts at: 4861 4861 Size: 5962 PflMI CCAn_nnn'nTGG Cuts at: 1108 2320 1108 Size: 1212 4750 PmeI GTTT'AAAC Cuts at: 3266 3266 Size: 5962 PmlI CAC'GTG Cuts at: 2558 2558 Size: 5962 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4031 4063 5544 5576 4031 Size: 32 1481 32 4417 Fragments arranged by size: 4417 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2164 2164 Size: 5962 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5962 PsiI TTA'TAA Cuts at: 3532 3532 Size: 5962 PstI C_TGCA'G Cuts at: 830 3314 830 Size: 2484 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5962 SalI G'TCGA_C Cuts at: 1936 3304 1936 Size: 1368 4594 SbfI CC_TGCA'GG Cuts at: 3314 3314 Size: 5962 ScaI AGT'ACT Cuts at: 3206 4210 5922 3206 Size: 1004 1712 3246 Fragments arranged by size: 3246 1712 1004 SfcI C'TryA_G Cuts at: 826 1045 3021 3310 4445 5126 5317 826 Size: 219 1976 289 1135 681 191 1471 Fragments arranged by size: 1976 1471 1135 681 289 219 191 SfoI GGC'GCC Cuts at: 3001 3001 Size: 5962 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5962 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5962 SmaI CCC'GGG Cuts at: 1872 3289 1872 Size: 1417 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2343 4066 4967 5229 820 Size: 197 926 400 1723 901 262 1553 Fragments arranged by size: 1723 1553 926 901 400 262 197 SnaBI TAC'GTA Cuts at: 493 3259 3753 493 Size: 2766 494 2702 Fragments arranged by size: 2766 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5962 SphI G_CATG'C Cuts at: 1132 3320 1132 Size: 2188 3774 SspI AAT'ATT Cuts at: 5 52 3886 5 Size: 47 3834 2081 Fragments arranged by size: 3834 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5962 StyI C'CwwG_G Cuts at: 513 1065 2022 2148 3401 513 Size: 552 957 126 1253 3074 Fragments arranged by size: 3074 1253 957 552 126 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2738 3989 4006 4159 4344 4789 325 Size: 1255 1158 1251 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1251 1158 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3102 3204 4208 Size: 274 80 33 51 1443 1125 102 1004 Cuts at: 4208 5920 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1125 1004 274 138 102 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5922 5954 5922 Size: 32 5930 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5962 XbaI T'CTAG_A Cuts at: 3298 3298 Size: 5962 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2038 2119 2371 2441 2887 2038 Size: 81 252 70 446 5113 Fragments arranged by size: 5113 446 252 81 70 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5962 XmaI C'CCGG_G Cuts at: 1870 3287 1870 Size: 1417 4545 XmnI GAAnn'nnTTC Cuts at: 2987 4091 2987 Size: 1104 4858 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3770 276 Size: 53 83 186 936 2236 2468 Fragments arranged by size: 2468 2236 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BplI Bpu10I BsiWI BsmBI EcoICRI FalI FseI FspAI NaeI NgoMIV NruI PacI PfoI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI