(Circular) (Six-base) MAPSORT of: pFN21ASDA0093.seq Check: 334 from: 1 to: 7429 pFN21ASDA0093 7429 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3244 3244 Size: 7429 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5239 278 Size: 53 83 186 936 3703 2468 Fragments arranged by size: 3703 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 4772 1937 Size: 2835 4594 Acc65I G'GTAC_C Cuts at: 4750 4750 Size: 7429 AclI AA'CG_TT Cuts at: 1563 5556 5929 1563 Size: 3993 373 3063 Fragments arranged by size: 3993 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3749 4478 4664 5492 6876 7144 1632 Size: 2117 729 186 828 1384 268 1917 Fragments arranged by size: 2117 1917 1384 828 729 268 186 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7232 AflIII A'CryG_T Cuts at: 1203 6328 7305 1203 Size: 5125 977 1327 Fragments arranged by size: 5125 1327 977 AgeI A'CCGG_T Cuts at: 6260 6260 Size: 7429 AhdI GACnn_n'nnGTC Cuts at: 6158 6158 Size: 7429 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 7429 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 4287 1184 Size: 3103 4326 AlwNI CAG_nnn'CTG Cuts at: 2561 2714 5172 6744 2561 Size: 153 2458 1572 3246 Fragments arranged by size: 3246 2458 1572 153 ApaLI G'TGCA_C Cuts at: 5486 6642 5486 Size: 1156 6273 ApoI r'AATT_y Cuts at: 1493 2071 2950 3387 3647 4320 4738 4964 5116 Size: 578 879 437 260 673 418 226 152 Cuts at: 5116 7225 1493 Size: 2109 1697 Fragments arranged by size: 2109 1697 879 673 578 437 418 260 226 152 AseI AT'TA_AT Cuts at: 160 1032 5983 160 Size: 872 4951 1606 Fragments arranged by size: 4951 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7429 AvaI C'yCGr_G Cuts at: 1870 1943 4515 4754 1870 Size: 73 2572 239 4545 Fragments arranged by size: 4545 2572 239 73 AvrII C'CTAG_G Cuts at: 2329 2329 Size: 7429 BamHI G'GATC_C Cuts at: 4759 5126 4759 Size: 367 7062 BanI G'GyrC_C Cuts at: 618 943 1160 1787 4519 4750 6205 618 Size: 325 217 627 2732 231 1455 1842 Fragments arranged by size: 2732 1842 1455 627 325 231 217 BanII G_rGCy'C Cuts at: 1391 2055 2981 4615 4748 1391 Size: 664 926 1634 133 4072 Fragments arranged by size: 4072 1634 926 664 133 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2799 3690 928 Size: 971 900 891 4667 Fragments arranged by size: 4667 971 900 891 BbvCI CC'TCA_GC Cuts at: 2268 2517 2268 Size: 249 7180 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5620 5654 1903 Size: 34 3683 34 3678 Fragments arranged by size: 3683 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 5321 6531 5321 Size: 1210 6219 BclI T'GATC_A Cuts at: 1548 1548 Size: 7429 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6040 136 Size: 107 122 71 631 605 4368 1525 Fragments arranged by size: 4368 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 7424 7424 Size: 7429 BlpI GC'TnA_GC Cuts at: 4846 4846 Size: 7429 Bme1580I G_kGCm'C Cuts at: 1790 3573 5490 6646 1790 Size: 1783 1917 1156 2573 Fragments arranged by size: 2573 1917 1783 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 7429 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2827 4370 5160 Size: 416 53 83 399 24 1410 1543 790 Cuts at: 5160 6118 442 Size: 958 1753 Fragments arranged by size: 1753 1543 1410 958 790 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7429 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2078 2589 3435 3562 4405 6089 1239 Size: 839 511 846 127 843 1684 2579 Fragments arranged by size: 2579 1684 846 843 839 511 127 Bpu10I CC'TnA_GC Cuts at: 2268 2517 2268 Size: 249 7180 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3181 5554 6419 6717 3181 Size: 2373 865 298 3893 Fragments arranged by size: 3893 2373 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2380 3173 6092 882 Size: 617 881 793 2919 2219 Fragments arranged by size: 2919 2219 881 793 617 BsaAI yAC'GTr Cuts at: 493 1204 2152 4726 5220 493 Size: 711 948 2574 494 2702 Fragments arranged by size: 2702 2574 948 711 494 BsaBI GATnn'nnATC Cuts at: 7423 7423 Size: 7429 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5236 5618 6253 275 Size: 53 83 186 936 3703 382 635 1451 Fragments arranged by size: 3703 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3693 5862 6260 6534 6681 7092 3693 Size: 2169 398 274 147 411 4030 Fragments arranged by size: 4030 2169 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 7204 Fragments arranged by size: 7204 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2586 3795 1186 Size: 1400 1209 4820 Fragments arranged by size: 4820 1400 1209 BseYI C'CCAG_C Cuts at: 2535 3261 3300 6632 7128 2535 Size: 726 39 3332 496 2836 Fragments arranged by size: 3332 2836 726 496 39 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 6856 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4879 5640 6668 1540 Size: 275 186 2878 761 1028 2301 Fragments arranged by size: 2878 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2981 4615 5490 5575 6646 2981 Size: 1634 875 85 1071 3764 Fragments arranged by size: 3764 1634 1071 875 85 BsmI GAATG_Cn' Cuts at: 2932 4940 5033 2932 Size: 2008 93 5328 Fragments arranged by size: 5328 2008 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2055 2981 3573 4615 4748 5490 5575 Size: 399 265 926 592 1042 133 742 85 Cuts at: 5575 6646 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 1042 926 742 592 399 265 133 85 BspEI T'CCGG_A Cuts at: 7092 7092 Size: 7429 BspHI T'CATG_A Cuts at: 4036 5318 7048 4036 Size: 1282 1730 4417 Fragments arranged by size: 4417 1730 1282 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3244 4784 844 Size: 2400 1540 3489 Fragments arranged by size: 3489 2400 1540 BsrBI CCG'CTC Cuts at: 1394 1823 5316 1394 Size: 429 3493 3507 Fragments arranged by size: 3507 3493 429 BsrDI GCAATG_nn' Cuts at: 65 1248 5924 6098 65 Size: 1183 4676 174 1396 Fragments arranged by size: 4676 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2277 6073 6260 1700 Size: 110 467 3796 187 2869 Fragments arranged by size: 3796 2869 467 187 110 BsrGI T'GTAC_A Cuts at: 96 3163 96 Size: 3067 4362 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7429 BssSI C'ACGA_G Cuts at: 5489 6501 5489 Size: 1012 6417 BstAPI GCAn_nnn'nTGC Cuts at: 794 2747 794 Size: 1953 5476 BstBI TT'CG_AA Cuts at: 2007 6233 2007 Size: 4226 3203 BstEII G'GTnAC_C Cuts at: 1184 3256 1184 Size: 2072 5357 BstXI CCAn_nnnn'nTGG Cuts at: 3081 3081 Size: 7429 BstYI r'GATC_y Cuts at: 1972 3373 3529 4309 4759 5126 5511 5528 6980 Size: 1401 156 780 450 367 385 17 1452 Cuts at: 6980 7066 7078 7424 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 1401 780 450 385 367 346 156 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2694 4115 2694 Size: 1421 6008 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 5920 Fragments arranged by size: 5920 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 6690 Fragments arranged by size: 6690 683 56 BtsI GCAGTG_nn' Cuts at: 3573 4945 5739 5759 3573 Size: 1372 794 20 5243 Fragments arranged by size: 5243 1372 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7429 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 7394 DraI TTT'AAA Cuts at: 4279 4733 4279 Size: 454 6975 DraIII CAC_nnn'GTG Cuts at: 2747 2747 Size: 7429 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6436 809 Size: 507 5120 1802 Fragments arranged by size: 5120 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 4876 5765 7394 Size: 54 1425 50 275 18 3046 889 1629 Cuts at: 7394 8 Size: 43 Fragments arranged by size: 3046 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 4876 1537 Size: 275 3064 4090 Fragments arranged by size: 4090 3064 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2610 2992 3456 5359 1353 Size: 1257 382 464 1903 3423 Fragments arranged by size: 3423 1903 1257 464 382 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3983 6016 6390 6536 387 Size: 1064 138 2394 2033 374 146 1280 Fragments arranged by size: 2394 2033 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2979 4613 2979 Size: 1634 5795 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2078 2589 3435 3562 3749 4405 4478 Size: 393 446 511 846 127 187 656 73 Cuts at: 4478 4664 5492 6089 6876 7144 1239 Size: 186 828 597 787 268 1524 Fragments arranged by size: 1524 846 828 787 656 597 511 446 393 268 187 186 127 73 EcoNI CCTnn'n_nnAGG Cuts at: 3577 3577 Size: 7429 EcoO109I rG'GnC_Cy Cuts at: 2640 3215 3440 2640 Size: 575 225 6629 Fragments arranged by size: 6629 575 225 EcoRI G'AATT_C Cuts at: 4738 5116 4738 Size: 378 7051 EcoRV GAT'ATC Cuts at: 2629 2772 2629 Size: 143 7286 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3305 3305 Size: 7429 FspI TGC'GCA Cuts at: 5935 5935 Size: 7429 HaeII r_GCGC'y Cuts at: 1678 6576 1678 Size: 4898 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2212 2244 2356 2388 2968 Size: 32 135 32 640 32 112 32 580 Cuts at: 2968 3000 3902 3934 4031 4063 6070 6102 6144 Size: 32 902 32 97 32 2007 32 42 Cuts at: 6144 6176 1373 Size: 32 2626 Fragments arranged by size: 2626 2007 902 640 580 135 112 97 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2794 4773 5019 669 Size: 988 281 856 1979 246 3079 Fragments arranged by size: 3079 1979 988 856 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 7429 HpaI GTT'AAC Cuts at: 5019 5019 Size: 7429 KpnI G_GTAC'C Cuts at: 4754 4754 Size: 7429 MfeI C'AATT_G Cuts at: 4689 5028 4689 Size: 339 7090 MluI A'CGCG_T Cuts at: 7305 7305 Size: 7429 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2927 3519 3957 6543 6727 1305 Size: 1622 592 438 2586 184 2007 Fragments arranged by size: 2586 2007 1622 592 438 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 5607 Fragments arranged by size: 5607 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3477 4158 5387 5746 5905 518 Size: 648 2311 681 1229 359 159 2042 Fragments arranged by size: 2311 2042 1229 681 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3190 4845 5522 6670 6915 1606 Size: 1584 1655 677 1148 245 2120 Fragments arranged by size: 2120 1655 1584 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 5920 Fragments arranged by size: 5920 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 7429 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7429 NotI GC'GGCC_GC Cuts at: 4876 4876 Size: 7429 NspI r_CATG'y Cuts at: 1132 1229 3317 4787 6332 1132 Size: 97 2088 1470 1545 2229 Fragments arranged by size: 2229 2088 1545 1470 97 PasI CC'CwG_GG Cuts at: 1348 4702 1348 Size: 3354 4075 PciI A'CATG_T Cuts at: 6328 6328 Size: 7429 PflMI CCAn_nnn'nTGG Cuts at: 1108 2180 3366 3795 3893 1108 Size: 1072 1186 429 98 4644 Fragments arranged by size: 4644 1186 1072 429 98 PfoI T'CCnGG_A Cuts at: 2055 3451 2055 Size: 1396 6033 PmeI GTTT'AAAC Cuts at: 4733 4733 Size: 7429 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3660 3692 5498 5530 7011 7043 3660 Size: 32 1806 32 1481 32 4046 Fragments arranged by size: 4046 1806 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2640 2640 Size: 7429 PshAI GACnn'nnGTC Cuts at: 1613 4564 1613 Size: 2951 4478 PsiI TTA'TAA Cuts at: 2828 4999 2828 Size: 2171 5258 PstI C_TGCA'G Cuts at: 830 3214 4781 830 Size: 2384 1567 3478 Fragments arranged by size: 3478 2384 1567 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7429 PvuII CAG'CTG Cuts at: 3190 3190 Size: 7429 SacI G_AGCT'C Cuts at: 2981 4615 2981 Size: 1634 5795 SalI G'TCGA_C Cuts at: 1936 4771 1936 Size: 2835 4594 SbfI CC_TGCA'GG Cuts at: 4781 4781 Size: 7429 ScaI AGT'ACT Cuts at: 5677 7389 5677 Size: 1712 5717 SexAI A'CCwGG_T Cuts at: 3076 3076 Size: 7429 SfcI C'TryA_G Cuts at: 826 1045 3210 3875 4414 4777 5912 6593 6784 Size: 219 2165 665 539 363 1135 681 191 Cuts at: 6784 826 Size: 1471 Fragments arranged by size: 2165 1471 1135 681 665 539 363 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7429 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7429 SmaI CCC'GGG Cuts at: 1872 4756 1872 Size: 2884 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3196 5533 6434 6696 820 Size: 197 926 1253 2337 901 262 1553 Fragments arranged by size: 2337 1553 1253 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4726 5220 493 Size: 4233 494 2702 Fragments arranged by size: 4233 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7429 SphI G_CATG'C Cuts at: 1132 3317 4787 1132 Size: 2185 1470 3774 Fragments arranged by size: 3774 2185 1470 SspI AAT'ATT Cuts at: 5 52 2250 3851 4064 5353 5 Size: 47 2198 1601 213 1289 2081 Fragments arranged by size: 2198 2081 1601 1289 213 47 StuI AGG'CCT Cuts at: 1517 2112 2864 3331 1517 Size: 595 752 467 5615 Fragments arranged by size: 5615 752 595 467 StyI C'CwwG_G Cuts at: 513 1065 2022 2329 2632 4868 513 Size: 552 957 307 303 2236 3074 Fragments arranged by size: 3074 2236 957 552 307 303 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3695 4715 5456 5473 5626 5811 6256 Size: 1255 2115 1020 741 17 153 185 445 Cuts at: 6256 325 Size: 1498 Fragments arranged by size: 2115 1498 1255 1020 741 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3163 5675 7387 Size: 274 80 33 51 1443 1186 2512 1712 Cuts at: 7387 96 Size: 138 Fragments arranged by size: 2512 1712 1443 1186 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3552 3584 7389 7421 3552 Size: 32 3805 32 3560 Fragments arranged by size: 3805 3560 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 7429 XbaI T'CTAG_A Cuts at: 2663 4765 2663 Size: 2102 5327 XcmI CCAnnnn_n'nnnnTGG Cuts at: 4709 4709 Size: 7429 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7429 XmaI C'CCGG_G Cuts at: 1870 4754 1870 Size: 2884 4545 XmnI GAAnn'nnTTC Cuts at: 5558 5558 Size: 7429 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5237 276 Size: 53 83 186 936 3703 2468 Fragments arranged by size: 3703 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI RsrII SacII SanDI SapI SfiI SfoI SrfI SwaI