(Circular) (Six-base) MAPSORT of: pFN21ASDA0085.seq Check: 4162 from: 1 to: 5515 pFN21ASDA0085 5515 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3325 278 Size: 53 83 186 936 1789 2468 Fragments arranged by size: 2468 1789 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2631 2858 1937 Size: 694 227 4594 Fragments arranged by size: 4594 694 227 Acc65I G'GTAC_C Cuts at: 2836 2836 Size: 5515 AclI AA'CG_TT Cuts at: 1563 3642 4015 1563 Size: 2079 373 3063 Fragments arranged by size: 3063 2079 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3578 4962 5230 1632 Size: 1946 1384 268 1917 Fragments arranged by size: 1946 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5318 AflIII A'CryG_T Cuts at: 1203 2634 4414 5391 1203 Size: 1431 1780 977 1327 Fragments arranged by size: 1780 1431 1327 977 AgeI A'CCGG_T Cuts at: 4346 4346 Size: 5515 AhdI GACnn_n'nnGTC Cuts at: 4244 4244 Size: 5515 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5515 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5515 AlwNI CAG_nnn'CTG Cuts at: 2446 3258 4830 2446 Size: 812 1572 3131 Fragments arranged by size: 3131 1572 812 ApaI G_GGCC'C Cuts at: 2783 2783 Size: 5515 ApaLI G'TGCA_C Cuts at: 3572 4728 3572 Size: 1156 4359 ApoI r'AATT_y Cuts at: 1493 2824 3050 3202 5311 1493 Size: 1331 226 152 2109 1697 Fragments arranged by size: 2109 1697 1331 226 152 AseI AT'TA_AT Cuts at: 160 1032 4069 160 Size: 872 3037 1606 Fragments arranged by size: 3037 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5515 AvaI C'yCGr_G Cuts at: 1870 1943 2840 1870 Size: 73 897 4545 Fragments arranged by size: 4545 897 73 BamHI G'GATC_C Cuts at: 2845 3212 2845 Size: 367 5148 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2836 4291 618 Size: 325 217 627 1049 1455 1842 Fragments arranged by size: 1842 1455 1049 627 325 217 BanII G_rGCy'C Cuts at: 1391 2360 2783 2834 1391 Size: 969 423 51 4072 Fragments arranged by size: 4072 969 423 51 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2771 928 Size: 971 872 3672 Fragments arranged by size: 3672 971 872 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3706 3740 1903 Size: 34 1769 34 3678 Fragments arranged by size: 3678 1769 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3407 4617 3407 Size: 1210 4305 BclI T'GATC_A Cuts at: 1548 2109 2586 2667 1548 Size: 561 477 81 4396 Fragments arranged by size: 4396 561 477 81 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4126 136 Size: 107 122 71 631 605 2454 1525 Fragments arranged by size: 2454 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5510 5510 Size: 5515 BlpI GC'TnA_GC Cuts at: 2932 2932 Size: 5515 Bme1580I G_kGCm'C Cuts at: 1790 2499 2783 3576 4732 1790 Size: 709 284 793 1156 2573 Fragments arranged by size: 2573 1156 793 709 284 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5515 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3246 4204 442 Size: 416 53 83 399 24 1829 958 1753 Fragments arranged by size: 1829 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2412 1056 Size: 1356 4159 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2200 2699 4175 1239 Size: 961 499 1476 2579 Fragments arranged by size: 2579 1476 961 499 Bpu10I CC'TnA_GC Cuts at: 2255 2459 2255 Size: 204 5311 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2369 3640 4505 4803 2369 Size: 1271 865 298 3081 Fragments arranged by size: 3081 1271 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4178 882 Size: 617 2679 2219 Fragments arranged by size: 2679 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2812 3306 493 Size: 711 1608 494 2702 Fragments arranged by size: 2702 1608 711 494 BsaBI GATnn'nnATC Cuts at: 2585 2588 5509 2585 Size: 3 2921 2591 Fragments arranged by size: 2921 2591 3 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2165 3322 3704 4339 Size: 53 83 186 936 632 1157 382 635 Cuts at: 4339 275 Size: 1451 Fragments arranged by size: 1451 1157 936 635 632 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3948 4346 4620 4767 5178 3948 Size: 398 274 147 411 4285 Fragments arranged by size: 4285 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2198 2228 1178 Size: 30 165 30 795 30 4465 Fragments arranged by size: 4465 795 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2404 2705 1186 Size: 1218 301 3996 Fragments arranged by size: 3996 1218 301 BseYI C'CCAG_C Cuts at: 2353 2543 2782 4718 5214 2353 Size: 190 239 1936 496 2654 Fragments arranged by size: 2654 1936 496 239 190 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4942 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2965 3726 4754 1540 Size: 275 186 964 761 1028 2301 Fragments arranged by size: 2301 1028 964 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2139 3576 3661 4732 2139 Size: 1437 85 1071 2922 Fragments arranged by size: 2922 1437 1071 85 BsmI GAATG_Cn' Cuts at: 3026 3119 3026 Size: 93 5422 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2139 2360 2499 2783 2834 3576 3661 Size: 399 349 221 139 284 51 742 85 Cuts at: 3661 4732 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 399 349 284 221 139 85 51 BspEI T'CCGG_A Cuts at: 5178 5178 Size: 5515 BspHI T'CATG_A Cuts at: 2112 3404 5134 2112 Size: 1292 1730 2493 Fragments arranged by size: 2493 1730 1292 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2063 2304 2870 844 Size: 1219 241 566 3489 Fragments arranged by size: 3489 1219 566 241 BsrBI CCG'CTC Cuts at: 1394 1823 3402 1394 Size: 429 1579 3507 Fragments arranged by size: 3507 1579 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4010 4184 65 Size: 1183 2762 174 1396 Fragments arranged by size: 2762 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4159 4346 1700 Size: 110 2349 187 2869 Fragments arranged by size: 2869 2349 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5515 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5515 BssSI C'ACGA_G Cuts at: 3575 4587 3575 Size: 1012 4503 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5515 BstBI TT'CG_AA Cuts at: 2007 4319 2007 Size: 2312 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5515 BstXI CCAn_nnnn'nTGG Cuts at: 2092 2092 Size: 5515 BstYI r'GATC_y Cuts at: 1972 2845 3212 3597 3614 5066 5152 5164 5510 Size: 873 367 385 17 1452 86 12 346 Cuts at: 5510 1972 Size: 1977 Fragments arranged by size: 1977 1452 873 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2195 2195 Size: 5515 BtgI C'CryG_G Cuts at: 513 1065 2022 2155 513 Size: 552 957 133 3873 Fragments arranged by size: 3873 957 552 133 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2062 2584 487 Size: 683 56 836 522 3418 Fragments arranged by size: 3418 836 683 522 56 BtsI GCAGTG_nn' Cuts at: 3031 3825 3845 3031 Size: 794 20 4701 Fragments arranged by size: 4701 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5515 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5480 DraI TTT'AAA Cuts at: 2819 2819 Size: 5515 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4522 809 Size: 507 3206 1802 Fragments arranged by size: 3206 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2094 2962 3851 Size: 54 1425 50 275 18 264 868 889 Cuts at: 3851 5480 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 868 275 264 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2962 1537 Size: 275 1150 4090 Fragments arranged by size: 4090 1150 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2246 2690 3445 1353 Size: 893 444 755 3423 Fragments arranged by size: 3423 893 755 444 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2036 4102 4476 4622 387 Size: 1064 138 447 2066 374 146 1280 Fragments arranged by size: 2066 1280 1064 447 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2200 2699 3578 4175 4962 5230 1239 Size: 393 568 499 879 597 787 268 1524 Fragments arranged by size: 1524 879 787 597 568 499 393 268 EcoO109I rG'GnC_Cy Cuts at: 2779 2779 Size: 5515 EcoRI G'AATT_C Cuts at: 2824 3202 2824 Size: 378 5137 FspI TGC'GCA Cuts at: 4021 4021 Size: 5515 HaeII r_GCGC'y Cuts at: 1678 4662 1678 Size: 2984 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2198 2230 4156 4188 4230 Size: 32 135 32 626 32 1926 32 42 Cuts at: 4230 4262 1373 Size: 32 2626 Fragments arranged by size: 2626 1926 626 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2859 3105 669 Size: 988 281 921 246 3079 Fragments arranged by size: 3079 988 921 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5515 HpaI GTT'AAC Cuts at: 3105 3105 Size: 5515 KpnI G_GTAC'C Cuts at: 2840 2840 Size: 5515 MfeI C'AATT_G Cuts at: 3114 3114 Size: 5515 MluI A'CGCG_T Cuts at: 5391 5391 Size: 5515 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4629 4813 1305 Size: 3324 184 2007 Fragments arranged by size: 3324 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2096 10 Size: 54 1425 343 264 3429 Fragments arranged by size: 3429 1425 343 264 54 MslI CAynn'nnrTG Cuts at: 518 1166 2234 2492 2579 3473 3832 3991 518 Size: 648 1068 258 87 894 359 159 2042 Fragments arranged by size: 2042 1068 894 648 359 258 159 87 MspA1I CmG'CkG Cuts at: 1606 2443 2931 3608 4756 5001 1606 Size: 837 488 677 1148 245 2120 Fragments arranged by size: 2120 1148 837 677 488 245 NcoI C'CATG_G Cuts at: 513 1065 2022 2155 513 Size: 552 957 133 3873 Fragments arranged by size: 3873 957 552 133 NdeI CA'TA_TG Cuts at: 387 387 Size: 5515 NheI G'CTAG_C Cuts at: 1052 2408 1052 Size: 1356 4159 NotI GC'GGCC_GC Cuts at: 2962 2962 Size: 5515 NspI r_CATG'y Cuts at: 1132 1229 2638 2873 4418 1132 Size: 97 1409 235 1545 2229 Fragments arranged by size: 2229 1545 1409 235 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5515 PciI A'CATG_T Cuts at: 2634 4414 2634 Size: 1780 3735 PflMI CCAn_nnn'nTGG Cuts at: 1108 2722 1108 Size: 1614 3901 PfoI T'CCnGG_A Cuts at: 2396 2396 Size: 5515 PmeI GTTT'AAAC Cuts at: 2819 2819 Size: 5515 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3584 3616 5097 5129 3584 Size: 32 1481 32 3970 Fragments arranged by size: 3970 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5515 PsiI TTA'TAA Cuts at: 3085 3085 Size: 5515 PspOMI G'GGCC_C Cuts at: 2779 2779 Size: 5515 PstI C_TGCA'G Cuts at: 830 2442 2480 2867 830 Size: 1612 38 387 3478 Fragments arranged by size: 3478 1612 387 38 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5515 PvuII CAG'CTG Cuts at: 2443 2443 Size: 5515 SalI G'TCGA_C Cuts at: 1936 2857 1936 Size: 921 4594 SapI GCTCTTCn'nnn_ Cuts at: 2246 2246 Size: 5515 SbfI CC_TGCA'GG Cuts at: 2867 2867 Size: 5515 ScaI AGT'ACT Cuts at: 3763 5475 3763 Size: 1712 3803 SfcI C'TryA_G Cuts at: 826 1045 2438 2476 2659 2863 3998 4679 4870 Size: 219 1393 38 183 204 1135 681 191 Cuts at: 4870 826 Size: 1471 Fragments arranged by size: 1471 1393 1135 681 219 204 191 183 38 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5515 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5515 SmaI CCC'GGG Cuts at: 1872 2842 1872 Size: 970 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2384 3619 4520 4782 820 Size: 197 926 441 1235 901 262 1553 Fragments arranged by size: 1553 1235 926 901 441 262 197 SnaBI TAC'GTA Cuts at: 493 2812 3306 493 Size: 2319 494 2702 Fragments arranged by size: 2702 2319 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 5515 SphI G_CATG'C Cuts at: 1132 2873 1132 Size: 1741 3774 SspI AAT'ATT Cuts at: 5 52 3439 5 Size: 47 3387 2081 Fragments arranged by size: 3387 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5515 StyI C'CwwG_G Cuts at: 513 1065 2022 2155 2954 513 Size: 552 957 133 799 3074 Fragments arranged by size: 3074 957 799 552 133 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3542 3559 3712 3897 4342 325 Size: 1255 1962 17 153 185 445 1498 Fragments arranged by size: 1962 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3761 5473 96 Size: 274 80 33 51 1443 1784 1712 138 Fragments arranged by size: 1784 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5475 5507 5475 Size: 32 5483 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5515 XbaI T'CTAG_A Cuts at: 2851 2851 Size: 5515 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5515 XmaI C'CCGG_G Cuts at: 1870 2840 1870 Size: 970 4545 XmnI GAAnn'nnTTC Cuts at: 3644 3644 Size: 5515 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3323 276 Size: 53 83 186 936 1789 2468 Fragments arranged by size: 2468 1789 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI