Restriction map for
pFN21ASDA0084
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# NO CUT ENZYMES ( 124 enzymes) #
##################################
AatII Acc65I AclI AflII AgeI
AhdI Alw44I Aor51HI ApaLI AscI
AseI AsnI Asp700I Asp718I AspEI
AvaI AviII AvrII BamHI BbeI
BcgI BfaI BfrI BglI BlnI
BlpI Bpu1102I BsaBI BsiEI BsiWI
BsmBI BsmI BsoBI Bsp1407I BspDI
BspHI BspLU11I BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstPI BstXI BstZ17I CelII CfoI
ClaI CpoI DraI DraIII DrdI
DsaI EagI Eam1105I EclXI Eco47III
Eco52I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FseI FspI HaeII HhaI
HinP1I HincII HindII HindIII HpaI
KasI KpnI KspI MaeI MamI
MfeI MunI NarI NcoI NdeI
NheI NotI NruI NsiI NspV
PacI PaeR7I PinAI PmeI PshAI
PshBI Psp1406I PstI PvuI RcaI
RsrII SacII SalI SbfI ScaI
SexAI SfcI SfiI SfoI SfuI
SgrAI SmaI SmlI SnaBI SpeI
Sse8387I SspBI SwaI XbaI XcmI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AccII 591 CGCG CG^CG
AcsI 469 AAATTC [GA]^AATT[CT]
AflIII 590 ACGCGT A^C[GA][CT]GT
AocI 76 CCTCAGG CC^T[ACGT]AGG
ApoI 469 AAATTC [GA]^AATT[CT]
AspI 1292 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BalI 921 TGGCCA TGG^CCA
BanI 690 GGTGCC G^G[CT][GA]CC
BbrPI 578 CACGTG CAC^GTG
BciVI 1261 GTATCC GTATCC(6/5)
BclI 314 TGATCA T^GATCA
BcnI 613 CCGGG CC^[GC]GG
BsaI 1290 GAGACC GGTCTC(1/5)
BseRI 1516 GAGGAG GAGGAG(10/8)
BspMI 191 ACCTGC ACCTGC(4/8)
BsrFI 875 GCCGGC [GA]^CCGG[CT]
BstUI 591 CGCG CG^CG
Bsu36I 76 CCTCAGG CC^T[ACGT]AGG
Cfr10I 875 GCCGGC [GA]^CCGG[CT]
EaeI 921 TGGCCA [CT]^GGCC[GA]
Eco81I 76 CCTCAGG CC^T[ACGT]AGG
FbaI 314 TGATCA T^GATCA
MluI 590 ACGCGT A^CGCGT
MluNI 921 TGGCCA TGG^CCA
MscI 921 TGGCCA TGG^CCA
MseI 434 TTAA T^TAA
MspA1I 1555 CAGCTG C[AC]G^C[GT]G
MvnI 591 CGCG CG^CG
NaeI 875 GCCGGC GCC^GGC
NciI 613 CCGGG CC^[GC]GG
NgoMIV 875 GCCGGC G^CCGGC
NspI 989 GCATGC [GA]CATG^[CT]
PflFI 1292 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 201 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PmaCI 578 CACGTG CAC^GTG
PmlI 578 CACGTG CAC^GTG
PpuMI 157 GGGTCCT [GA]G^G[AT]CC[CT]
PvuII 1555 CAGCTG CAG^CTG
SacI 148 GAGCTC GAGCT^C
SphI 989 GCATGC GCATG^C
SspI 52 AATATT AAT^ATT
StuI 73 AGGCCT AGG^CCT
Tru9I 434 TTAA T^TAA
Tth111I 1292 GACCTTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 201 CCAGCTCCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
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# TWO CUT ENZYMES ( 24 enzymes) #
###################################
AccI 534 GTCTAC GT^[AC][GT]AC
1298 GTCTAC
AccIII 721 TCCGGA T^CCGGA
977 TCCGGA
AcyI 282 GACGCC G[GA]^CG[CT]C
943 GACGCC
AlwI 441 GATCC GGATC(4/5)
585 GGATC
AlwNI 202 CAGCTCCTG CAG[ACGT][ACGT][ACGT]^CTG
460 CAGGGCCTG
ApaI 1527 GGGCCC GGGCC^C
1634 GGGCCC
BbvCI 161 CCTCAGC CCTCAGC(-5/-2)
1696 GCTGAGG
BglII 331 AGATCT A^GATCT
833 AGATCT
BsaAI 578 CACGTG [CT]AC^GT[GA]
1168 TACGTG
BsaHI 282 GACGCC G[GA]^CG[CT]C
943 GACGCC
BseAI 721 TCCGGA T^CCGGA
977 TCCGGA
BspEI 721 TCCGGA T^CCGGA
977 TCCGGA
BsrBI 271 GAGCGG CCGCTC(-3/-3)
933 GAGCGG
EcoT14I 794 CCTTGG C^C[AT][AT]GG
1014 CCTTGG
FauI 196 CCCGC CCCGC(4/6)
273 GCGGG
Hin1I 282 GACGCC G[GA]^CG[CT]C
943 GACGCC
MroI 721 TCCGGA T^CCGGA
977 TCCGGA
MslI 990 CATGCTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1212 CACCTGTGTG
PleI 132 GACTC GAGTC(4/5)
1508 GAGTC
SapI 808 GAAGAGC GCTCTTC(1/4)
858 GAAGAGC
StyI 794 CCTTGG C^C[AT][AT]GG
1014 CCTTGG
TaqI 1151 TCGA T^CGA
1327 TCGA
Tsp509I 374 AATT ^AATT
470 AATT
TthHB8I 1151 TCGA T^CGA
1327 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 65 enzymes) #
#########################################
AciI AfaI AluI AspHI AvaII
BanII BbsI BbvI BmyI BpmI
BpuAI BsaJI BsaWI BsgI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII DraII
EarI EcoO109I EcoRII Fnu4HI FokI
HaeIII HapII HgaI HinfI HpaII
HphI ItaI Ksp632I MaeII MaeIII
MboI MboII MflI MnlI MspI
MvaI MwoI NdeII NlaIII NlaIV
RsaI Sau3AI Sau96I ScrFI SfaNI
TfiI TseI Tsp45I TspRI XhoII