(Circular) (Six-base) MAPSORT of: pFN21ASDA0084.seq Check: 4668 from: 1 to: 6463 pFN21ASDA0084 6463 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4273 278 Size: 53 83 186 936 2737 2468 Fragments arranged by size: 2737 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2558 3322 3806 1937 Size: 621 764 484 4594 Fragments arranged by size: 4594 764 621 484 Acc65I G'GTAC_C Cuts at: 3784 3784 Size: 6463 AclI AA'CG_TT Cuts at: 1563 4590 4963 1563 Size: 3027 373 3063 Fragments arranged by size: 3063 3027 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3293 4526 5910 6178 1632 Size: 1661 1233 1384 268 1917 Fragments arranged by size: 1917 1661 1384 1233 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6266 AflIII A'CryG_T Cuts at: 1203 2613 5362 6339 1203 Size: 1410 2749 977 1327 Fragments arranged by size: 2749 1410 1327 977 AgeI A'CCGG_T Cuts at: 5294 5294 Size: 6463 AhdI GACnn_n'nnGTC Cuts at: 5192 5192 Size: 6463 AleI CACnn'nnGTG Cuts at: 1166 3239 1166 Size: 2073 4390 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6463 AlwNI CAG_nnn'CTG Cuts at: 2230 2488 4206 5778 2230 Size: 258 1718 1572 2915 Fragments arranged by size: 2915 1718 1572 258 ApaI G_GGCC'C Cuts at: 3554 3661 3554 Size: 107 6356 ApaLI G'TGCA_C Cuts at: 4520 5676 4520 Size: 1156 5307 ApoI r'AATT_y Cuts at: 1493 2492 3772 3998 4150 6259 1493 Size: 999 1280 226 152 2109 1697 Fragments arranged by size: 2109 1697 1280 999 226 152 AseI AT'TA_AT Cuts at: 160 1032 5017 160 Size: 872 3985 1606 Fragments arranged by size: 3985 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6463 AvaI C'yCGr_G Cuts at: 1870 1943 3788 1870 Size: 73 1845 4545 Fragments arranged by size: 4545 1845 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2498 2531 2498 Size: 33 6430 BamHI G'GATC_C Cuts at: 3793 4160 3793 Size: 367 6096 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2713 3784 5239 618 Size: 325 217 627 926 1071 1455 1842 Fragments arranged by size: 1842 1455 1071 926 627 325 217 BanII G_rGCy'C Cuts at: 1391 2175 2691 3554 3661 3782 1391 Size: 784 516 863 107 121 4072 Fragments arranged by size: 4072 863 784 516 121 107 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2309 3022 3468 3474 928 Size: 971 410 713 446 6 3917 Fragments arranged by size: 3917 971 713 446 410 6 BbvCI CC'TCA_GC Cuts at: 2185 3720 2185 Size: 1535 4928 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4654 4688 1903 Size: 34 2717 34 3678 Fragments arranged by size: 3678 2717 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3295 4355 5565 3295 Size: 1060 1210 4193 Fragments arranged by size: 4193 1210 1060 BclI T'GATC_A Cuts at: 1548 2337 1548 Size: 789 5674 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 5074 136 Size: 107 122 71 631 605 3402 1525 Fragments arranged by size: 3402 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2354 2856 6458 2354 Size: 502 3602 2359 Fragments arranged by size: 3602 2359 502 BlpI GC'TnA_GC Cuts at: 3880 3880 Size: 6463 Bme1580I G_kGCm'C Cuts at: 1790 2718 3198 3554 3661 3684 4524 5680 1790 Size: 928 480 356 107 23 840 1156 2573 Fragments arranged by size: 2573 1156 928 840 480 356 107 23 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6463 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2652 3687 4194 Size: 416 53 83 399 24 1235 1035 507 Cuts at: 4194 5152 442 Size: 958 1753 Fragments arranged by size: 1753 1235 1035 958 507 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6463 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2710 2857 2710 Size: 147 6316 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2165 2252 2333 2642 3355 3507 3714 5123 Size: 926 87 81 309 713 152 207 1409 Cuts at: 5123 1239 Size: 2579 Fragments arranged by size: 2579 1409 926 713 309 207 152 87 81 Bpu10I CC'TnA_GC Cuts at: 2185 2695 3720 2185 Size: 510 1025 4928 Fragments arranged by size: 4928 1025 510 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4588 5453 5751 4588 Size: 865 298 5300 Fragments arranged by size: 5300 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3307 5126 882 Size: 617 1808 1819 2219 Fragments arranged by size: 2219 1819 1808 617 BsaAI yAC'GTr Cuts at: 493 1204 2603 3193 3760 4254 493 Size: 711 1399 590 567 494 2702 Fragments arranged by size: 2702 1399 711 590 567 494 BsaBI GATnn'nnATC Cuts at: 6457 6457 Size: 6463 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2306 2967 4270 4652 Size: 53 83 186 936 773 661 1303 382 Cuts at: 4652 5287 275 Size: 635 1451 Fragments arranged by size: 1451 1303 936 773 661 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2551 2744 2803 3000 4896 5294 5568 5715 6126 Size: 193 59 197 1896 398 274 147 411 Cuts at: 6126 2551 Size: 2888 Fragments arranged by size: 2888 1896 411 398 274 197 193 147 59 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6238 Fragments arranged by size: 6238 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3554 1186 Size: 2368 4095 BseYI C'CCAG_C Cuts at: 2223 2666 2690 2732 5666 6162 2223 Size: 443 24 42 2934 496 2524 Fragments arranged by size: 2934 2524 496 443 42 24 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2252 2300 2674 3200 3650 1163 Size: 573 516 48 374 526 450 3976 Fragments arranged by size: 3976 573 526 516 450 374 48 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3913 4674 5702 1540 Size: 275 186 1912 761 1028 2301 Fragments arranged by size: 2301 1912 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2175 2319 2551 2662 2985 4524 4609 5680 2175 Size: 144 232 111 323 1539 85 1071 2958 Fragments arranged by size: 2958 1539 1071 323 232 144 111 85 BsmI GAATG_Cn' Cuts at: 3974 4067 3974 Size: 93 6370 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2175 2319 2551 2662 2691 2718 2985 Size: 399 385 144 232 111 29 27 267 Cuts at: 2985 3198 3554 3661 3684 3782 4524 4609 5680 Size: 213 356 107 23 98 742 85 1071 Cuts at: 5680 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 385 356 267 232 213 144 111 107 98 85 29 27 23 BspEI T'CCGG_A Cuts at: 2744 3000 6126 2744 Size: 256 3126 3081 Fragments arranged by size: 3126 3081 256 BspHI T'CATG_A Cuts at: 4352 6082 4352 Size: 1730 4733 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2223 3818 844 Size: 1379 1595 3489 Fragments arranged by size: 3489 1595 1379 BsrBI CCG'CTC Cuts at: 1394 1823 2296 2958 4350 1394 Size: 429 473 662 1392 3507 Fragments arranged by size: 3507 1392 662 473 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4958 5132 65 Size: 1183 3710 174 1396 Fragments arranged by size: 3710 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2898 5107 5294 1700 Size: 110 1088 2209 187 2869 Fragments arranged by size: 2869 2209 1088 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6463 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6463 BssSI C'ACGA_G Cuts at: 4523 5535 4523 Size: 1012 5451 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6463 BstBI TT'CG_AA Cuts at: 2007 5267 2007 Size: 3260 3203 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6463 BstYI r'GATC_y Cuts at: 1972 2354 2463 2856 3793 4160 4545 4562 6014 Size: 382 109 393 937 367 385 17 1452 Cuts at: 6014 6100 6112 6458 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 937 393 385 382 367 346 109 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2100 2100 Size: 6463 BtgI C'CryG_G Cuts at: 513 1065 2022 513 Size: 552 957 4954 Fragments arranged by size: 4954 957 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5724 Fragments arranged by size: 5724 683 56 BtsI GCAGTG_nn' Cuts at: 2196 2551 2662 3979 4773 4793 2196 Size: 355 111 1317 794 20 3866 Fragments arranged by size: 3866 1317 794 355 111 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6463 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6428 DraI TTT'AAA Cuts at: 3767 3767 Size: 6463 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5470 809 Size: 507 4154 1802 Fragments arranged by size: 4154 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2944 3910 4799 Size: 54 1425 50 275 18 1114 966 889 Cuts at: 4799 6428 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1114 966 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3910 1537 Size: 275 2098 4090 Fragments arranged by size: 4090 2098 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2087 2826 2876 4393 1353 Size: 734 739 50 1517 3423 Fragments arranged by size: 3423 1517 739 734 50 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5050 5424 5570 387 Size: 1064 138 3461 374 146 1280 Fragments arranged by size: 3461 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2173 2173 Size: 6463 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2165 2252 2333 2642 3293 3355 3507 Size: 393 533 87 81 309 651 62 152 Cuts at: 3507 3714 4526 5123 5910 6178 1239 Size: 207 812 597 787 268 1524 Fragments arranged by size: 1524 812 787 651 597 533 393 309 268 207 152 87 81 62 EcoO109I rG'GnC_Cy Cuts at: 2181 2485 2699 3550 3551 3657 3658 2181 Size: 304 214 851 1 106 1 4986 Fragments arranged by size: 4986 851 304 214 106 1 1 EcoRI G'AATT_C Cuts at: 3772 4150 3772 Size: 378 6085 FspI TGC'GCA Cuts at: 4969 4969 Size: 6463 HaeII r_GCGC'y Cuts at: 1678 5610 1678 Size: 3932 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2600 2632 3069 3101 3304 Size: 32 135 32 1028 32 437 32 203 Cuts at: 3304 3336 5104 5136 5178 5210 1373 Size: 32 1768 32 42 32 2626 Fragments arranged by size: 2626 1768 1028 437 203 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3807 4053 669 Size: 988 281 1869 246 3079 Fragments arranged by size: 3079 1869 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6463 HpaI GTT'AAC Cuts at: 4053 4053 Size: 6463 KpnI G_GTAC'C Cuts at: 3788 3788 Size: 6463 MfeI C'AATT_G Cuts at: 4062 4062 Size: 6463 MluI A'CGCG_T Cuts at: 2613 6339 2613 Size: 3726 2737 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5577 5761 1305 Size: 4272 184 2007 Fragments arranged by size: 4272 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2946 10 Size: 54 1425 343 1114 3527 Fragments arranged by size: 3527 1425 1114 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3017 3239 4421 4780 4939 518 Size: 648 1851 222 1182 359 159 2042 Fragments arranged by size: 2042 1851 1182 648 359 222 159 MspA1I CmG'CkG Cuts at: 1606 3580 3879 4556 5704 5949 1606 Size: 1974 299 677 1148 245 2120 Fragments arranged by size: 2120 1974 1148 677 299 245 NaeI GCC'GGC Cuts at: 2900 2900 Size: 6463 NcoI C'CATG_G Cuts at: 513 1065 2022 513 Size: 552 957 4954 Fragments arranged by size: 4954 957 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6463 NgoMIV G'CCGG_C Cuts at: 2898 2898 Size: 6463 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6463 NotI GC'GGCC_GC Cuts at: 3910 3910 Size: 6463 NspI r_CATG'y Cuts at: 1132 1229 3016 3821 5366 1132 Size: 97 1787 805 1545 2229 Fragments arranged by size: 2229 1787 1545 805 97 PasI CC'CwG_GG Cuts at: 1348 2177 1348 Size: 829 5634 PciI A'CATG_T Cuts at: 5362 5362 Size: 6463 PflMI CCAn_nnn'nTGG Cuts at: 1108 2230 1108 Size: 1122 5341 PfoI T'CCnGG_A Cuts at: 2142 2229 2466 3162 3691 2142 Size: 87 237 696 529 4914 Fragments arranged by size: 4914 696 529 237 87 PmeI GTTT'AAAC Cuts at: 3767 3767 Size: 6463 PmlI CAC'GTG Cuts at: 2603 2603 Size: 6463 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4532 4564 6045 6077 4532 Size: 32 1481 32 4918 Fragments arranged by size: 4918 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2181 2181 Size: 6463 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6463 PsiI TTA'TAA Cuts at: 4033 4033 Size: 6463 PspOMI G'GGCC_C Cuts at: 3550 3657 3550 Size: 107 6356 PstI C_TGCA'G Cuts at: 830 3815 830 Size: 2985 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6463 PvuII CAG'CTG Cuts at: 3580 3580 Size: 6463 SacI G_AGCT'C Cuts at: 2175 2175 Size: 6463 SalI G'TCGA_C Cuts at: 1936 3805 1936 Size: 1869 4594 SapI GCTCTTCn'nnn_ Cuts at: 2826 2876 2826 Size: 50 6413 SbfI CC_TGCA'GG Cuts at: 3815 3815 Size: 6463 ScaI AGT'ACT Cuts at: 4711 6423 4711 Size: 1712 4751 SfcI C'TryA_G Cuts at: 826 1045 3811 4946 5627 5818 826 Size: 219 2766 1135 681 191 1471 Fragments arranged by size: 2766 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6463 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6463 SmaI CCC'GGG Cuts at: 1872 3790 1872 Size: 1918 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 4567 5468 5730 820 Size: 197 926 2624 901 262 1553 Fragments arranged by size: 2624 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3760 4254 493 Size: 3267 494 2702 Fragments arranged by size: 3267 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 6463 SphI G_CATG'C Cuts at: 1132 3016 3821 1132 Size: 1884 805 3774 Fragments arranged by size: 3774 1884 805 SspI AAT'ATT Cuts at: 5 52 2077 4387 5 Size: 47 2025 2310 2081 Fragments arranged by size: 2310 2081 2025 47 StuI AGG'CCT Cuts at: 1517 2098 1517 Size: 581 5882 StyI C'CwwG_G Cuts at: 513 1065 2022 2817 3037 3902 513 Size: 552 957 795 220 865 3074 Fragments arranged by size: 3074 957 865 795 552 220 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2303 3599 4490 4507 4660 4845 5290 Size: 1255 723 1296 891 17 153 185 445 Cuts at: 5290 325 Size: 1498 Fragments arranged by size: 1498 1296 1255 891 723 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4709 6421 96 Size: 274 80 33 51 1443 2732 1712 138 Fragments arranged by size: 2732 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6423 6455 6423 Size: 32 6431 Tth111I GACn'n_nGTC Cuts at: 1316 3318 1316 Size: 2002 4461 XbaI T'CTAG_A Cuts at: 3799 3799 Size: 6463 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6463 XmaI C'CCGG_G Cuts at: 1870 3788 1870 Size: 1918 4545 XmnI GAAnn'nnTTC Cuts at: 4592 4592 Size: 6463 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4271 276 Size: 53 83 186 936 2737 2468 Fragments arranged by size: 2737 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BbeI BfrBI BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI