Restriction map for
pFN21ASDA0084
(Linear) (Six-base) MAPSORT of: KIAA0084.seq Check: 8024 from: 1 to: 1734
KIAA0084.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AccI GT'mk_AC
Cuts at: 0 535 1299 1734
Size: 535 764 435
Fragments arranged by size:
764 535 435
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1270 1734
Size: 1270 464
AflIII A'CryG_T
Cuts at: 0 590 1734
Size: 590 1144
AleI CACnn'nnGTG
Cuts at: 0 1216 1734
Size: 1216 518
AlwNI CAG_nnn'CTG
Cuts at: 0 207 465 1734
Size: 207 258 1269
Fragments arranged by size:
1269 258 207
ApaI G_GGCC'C
Cuts at: 0 1531 1638 1734
Size: 1531 107 96
Fragments arranged by size:
1531 107 96
ApoI r'AATT_y
Cuts at: 0 469 1734
Size: 469 1265
BaeI ACnnnnGTAyCnnnnnnn_nnnnn'
Cuts at: 0 475 508 1734
Size: 475 33 1226
Fragments arranged by size:
1226 475 33
BanI G'GyrC_C
Cuts at: 0 690 1734
Size: 690 1044
BanII G_rGCy'C
Cuts at: 0 152 668 1531 1638 1734
Size: 152 516 863 107 96
Fragments arranged by size:
863 516 152 107 96
BbsI GAAGACnn'nnnn_
Cuts at: 0 286 999 1445 1451 1734
Size: 286 713 446 6 283
Fragments arranged by size:
713 446 286 283 6
BbvCI CC'TCA_GC
Cuts at: 0 162 1697 1734
Size: 162 1535 37
Fragments arranged by size:
1535 162 37
BciVI GTATCCnnnnn_n'
Cuts at: 0 1272 1734
Size: 1272 462
BclI T'GATC_A
Cuts at: 0 314 1734
Size: 314 1420
BglII A'GATC_T
Cuts at: 0 331 833 1734
Size: 331 502 901
Fragments arranged by size:
901 502 331
Bme1580I G_kGCm'C
Cuts at: 0 695 1175 1531 1638 1661 1734
Size: 695 480 356 107 23 73
Fragments arranged by size:
695 480 356 107 73 23
BmrI ACTGGGnnnn_n'
Cuts at: 0 629 1664 1734
Size: 629 1035 70
Fragments arranged by size:
1035 629 70
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 687 834 1734
Size: 687 147 900
Fragments arranged by size:
900 687 147
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 229 310 619 1332 1484 1691 1734
Size: 142 87 81 309 713 152 207 43
Fragments arranged by size:
713 309 207 152 142 87 81 43
Bpu10I CC'TnA_GC
Cuts at: 0 162 672 1697 1734
Size: 162 510 1025 37
Fragments arranged by size:
1025 510 162 37
BsaI GGTCTCn'nnnn_
Cuts at: 0 1284 1734
Size: 1284 450
BsaAI yAC'GTr
Cuts at: 0 580 1170 1734
Size: 580 590 564
Fragments arranged by size:
590 580 564
BsaHI Gr'CG_yC
Cuts at: 0 283 944 1734
Size: 283 661 790
Fragments arranged by size:
790 661 283
BsaWI w'CCGG_w
Cuts at: 0 528 721 780 977 1734
Size: 528 193 59 197 757
Fragments arranged by size:
757 528 197 193 59
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1531 1734
Size: 1531 203
BseYI C'CCAG_C
Cuts at: 0 200 643 667 709 1734
Size: 200 443 24 42 1025
Fragments arranged by size:
1025 443 200 42 24
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 229 277 651 1177 1627 1734
Size: 229 48 374 526 450 107
Fragments arranged by size:
526 450 374 229 107 48
BsiHKAI G_wGCw'C
Cuts at: 0 152 296 528 639 962 1734
Size: 152 144 232 111 323 772
Fragments arranged by size:
772 323 232 152 144 111
Bsp1286I G_dGCh'C
Cuts at: 0 152 296 528 639 668 695 962 1175
Size: 152 144 232 111 29 27 267 213
Cuts at: 1175 1531 1638 1661 1734
Size: 356 107 23 73
Fragments arranged by size:
356 267 232 213 152 144 111 107
73 29 27 23
BspEI T'CCGG_A
Cuts at: 0 721 977 1734
Size: 721 256 757
Fragments arranged by size:
757 721 256
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 200 1734
Size: 200 1534
BsrBI CCG'CTC
Cuts at: 0 273 935 1734
Size: 273 662 799
Fragments arranged by size:
799 662 273
BsrFI r'CCGG_y
Cuts at: 0 875 1734
Size: 875 859
BstYI r'GATC_y
Cuts at: 0 331 440 833 1734
Size: 331 109 393 901
Fragments arranged by size:
901 393 331 109
Bsu36I CC'TnA_GG
Cuts at: 0 77 1734
Size: 77 1657
BtsI GCAGTG_nn'
Cuts at: 0 173 528 639 1734
Size: 173 355 111 1095
Fragments arranged by size:
1095 355 173 111
EaeI y'GGCC_r
Cuts at: 0 921 1734
Size: 921 813
EarI CTCTTCn'nnn_
Cuts at: 0 64 803 853 1734
Size: 64 739 50 881
Fragments arranged by size:
881 739 64 50
EcoICRI GAG'CTC
Cuts at: 0 150 1734
Size: 150 1584
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 142 229 310 619 1270 1332 1484 1691
Size: 142 87 81 309 651 62 152 207
Cuts at: 1691 1734
Size: 43
Fragments arranged by size:
651 309 207 152 142 87 81 62
43
EcoO109I rG'GnC_Cy
Cuts at: 0 158 462 676 1527 1528 1634 1635 1734
Size: 158 304 214 851 1 106 1 99
Fragments arranged by size:
851 304 214 158 106 99 1 1
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 577 609 1046 1078 1281 1313 1734
Size: 577 32 437 32 203 32 421
Fragments arranged by size:
577 437 421 203 32 32 32
MluI A'CGCG_T
Cuts at: 0 590 1734
Size: 590 1144
MscI TGG'CCA
Cuts at: 0 923 1734
Size: 923 811
MslI CAynn'nnrTG
Cuts at: 0 994 1216 1734
Size: 994 222 518
Fragments arranged by size:
994 518 222
MspA1I CmG'CkG
Cuts at: 0 1557 1734
Size: 1557 177
NaeI GCC'GGC
Cuts at: 0 877 1734
Size: 877 857
NgoMIV G'CCGG_C
Cuts at: 0 875 1734
Size: 875 859
NspI r_CATG'y
Cuts at: 0 993 1734
Size: 993 741
PasI CC'CwG_GG
Cuts at: 0 154 1734
Size: 154 1580
PflMI CCAn_nnn'nTGG
Cuts at: 0 207 1734
Size: 207 1527
PfoI T'CCnGG_A
Cuts at: 0 119 206 443 1139 1668 1734
Size: 119 87 237 696 529 66
Fragments arranged by size:
696 529 237 119 87 66
PmlI CAC'GTG
Cuts at: 0 580 1734
Size: 580 1154
PpuMI rG'GwC_Cy
Cuts at: 0 158 1734
Size: 158 1576
PspOMI G'GGCC_C
Cuts at: 0 1527 1634 1734
Size: 1527 107 100
Fragments arranged by size:
1527 107 100
PvuII CAG'CTG
Cuts at: 0 1557 1734
Size: 1557 177
SacI G_AGCT'C
Cuts at: 0 152 1734
Size: 152 1582
SapI GCTCTTCn'nnn_
Cuts at: 0 803 853 1734
Size: 803 50 881
Fragments arranged by size:
881 803 50
SphI G_CATG'C
Cuts at: 0 993 1734
Size: 993 741
SspI AAT'ATT
Cuts at: 0 54 1734
Size: 54 1680
StuI AGG'CCT
Cuts at: 0 75 1734
Size: 75 1659
StyI C'CwwG_G
Cuts at: 0 794 1014 1734
Size: 794 220 720
Fragments arranged by size:
794 720 220
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 280 1576 1734
Size: 280 1296 158
Fragments arranged by size:
1296 280 158
Tth111I GACn'n_nGTC
Cuts at: 0 1295 1734
Size: 1295 439
Enzymes that do cut:
AccI AcuI AflIII AleI AlwNI ApaI ApoI BaeI
BanI BanII BbsI BbvCI BciVI BclI BglII Bme1580I
BmrI BplI BpmI Bpu10I BsaI BsaAI BsaHI BsaWI
BseRI BseYI BsgI BsiHKAI Bsp1286I BspEI BspMI BsrBI
BsrFI BstYI Bsu36I BtsI EaeI EarI EcoICRI Eco57MI
EcoO109I Hin4I MluI MscI MslI MspA1I NaeI NgoMIV
NspI PasI PflMI PfoI PmlI PpuMI PspOMI PvuII
SacI SapI SphI SspI StuI StyI TaqII Tth111I
Enzymes that do not cut:
AarI AatII Acc65I AclI AfeI AflII AgeI AhdI
AlfI AloI ApaLI AscI AseI AsiSI AvaI AvrII
BamHI BbeI BcgI BfrBI BglI BlpI BmgBI BmtI
BpuEI BsaBI BsaXI BsiEI BsiWI BsmI BsmBI BspHI
BsrDI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI
BstZ17I BtgI BtgZI ClaI CspCI DraI DraIII DrdI
EagI EciI EcoNI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MmeI NarI NcoI NdeI NheI NotI NruI NsiI
PacI PciI PmeI PpiI PshAI PsiI PspXI PsrI
PstI PvuI RsrII SacII SalI SanDI SbfI ScaI
SexAI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SrfI SwaI TatI TstI XbaI XcmI
XhoI XmaI XmnI ZraI