(Circular) (Six-base) MAPSORT of: pFN21ASDA0028.seq Check: 2659 from: 1 to: 7438 pFN21ASDA0028 7438 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2668 2668 Size: 7438 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 5248 278 Size: 53 83 186 936 3712 2468 Fragments arranged by size: 3712 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2174 2334 4781 1937 Size: 237 160 2447 4594 Fragments arranged by size: 4594 2447 237 160 Acc65I G'GTAC_C Cuts at: 4759 4759 Size: 7438 AclI AA'CG_TT Cuts at: 1563 5565 5938 1563 Size: 4002 373 3063 Fragments arranged by size: 4002 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2952 3023 3228 3501 3598 5501 6885 7153 Size: 1320 71 205 273 97 1903 1384 268 Cuts at: 7153 1632 Size: 1917 Fragments arranged by size: 1917 1903 1384 1320 273 268 205 97 71 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 7241 AflIII A'CryG_T Cuts at: 1203 2549 2784 6337 7314 1203 Size: 1346 235 3553 977 1327 Fragments arranged by size: 3553 1346 1327 977 235 AgeI A'CCGG_T Cuts at: 6269 6269 Size: 7438 AhdI GACnn_n'nnGTC Cuts at: 6167 6167 Size: 7438 AleI CACnn'nnGTG Cuts at: 1166 3434 3659 1166 Size: 2268 225 4945 Fragments arranged by size: 4945 2268 225 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 7438 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4499 4531 4499 Size: 32 7406 AlwNI CAG_nnn'CTG Cuts at: 2252 4171 5181 6753 2252 Size: 1919 1010 1572 2937 Fragments arranged by size: 2937 1919 1572 1010 ApaLI G'TGCA_C Cuts at: 5495 6651 5495 Size: 1156 6282 ApoI r'AATT_y Cuts at: 1493 2241 2270 3147 4270 4578 4625 4747 4973 Size: 748 29 877 1123 308 47 122 226 Cuts at: 4973 5125 7234 1493 Size: 152 2109 1697 Fragments arranged by size: 2109 1697 1123 877 748 308 226 152 122 47 29 AseI AT'TA_AT Cuts at: 160 1032 5992 160 Size: 872 4960 1606 Fragments arranged by size: 4960 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 7438 AvaI C'yCGr_G Cuts at: 1870 1943 3312 4346 4602 4696 4763 1870 Size: 73 1369 1034 256 94 67 4545 Fragments arranged by size: 4545 1369 1034 256 94 73 67 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2714 2747 3576 3609 2714 Size: 33 829 33 6543 Fragments arranged by size: 6543 829 33 33 BamHI G'GATC_C Cuts at: 4768 5135 4768 Size: 367 7071 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2945 4438 4759 6214 618 Size: 325 217 627 1158 1493 321 1455 1842 Fragments arranged by size: 1842 1493 1455 1158 627 325 321 217 BanII G_rGCy'C Cuts at: 1391 4757 1391 Size: 3366 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 6467 BbvCI CC'TCA_GC Cuts at: 4164 4191 4164 Size: 27 7411 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 5629 5663 1903 Size: 34 3692 34 3678 Fragments arranged by size: 3692 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3577 5330 6540 3577 Size: 1753 1210 4475 Fragments arranged by size: 4475 1753 1210 BclI T'GATC_A Cuts at: 1548 3760 4554 1548 Size: 2212 794 4432 Fragments arranged by size: 4432 2212 794 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2921 6049 136 Size: 107 122 71 631 605 1249 3128 1525 Fragments arranged by size: 3128 1525 1249 631 605 122 107 71 BglII A'GATC_T Cuts at: 4416 7433 4416 Size: 3017 4421 BlpI GC'TnA_GC Cuts at: 4855 4855 Size: 7438 Bme1580I G_kGCm'C Cuts at: 1790 2994 3392 3441 4372 4459 4590 5499 6655 Size: 1204 398 49 931 87 131 909 1156 Cuts at: 6655 1790 Size: 2573 Fragments arranged by size: 2573 1204 1156 931 909 398 131 87 49 BmgBI CAC'GTC Cuts at: 1318 3337 3930 1318 Size: 2019 593 4826 Fragments arranged by size: 4826 2019 593 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2642 2650 3147 Size: 416 53 83 399 24 1225 8 497 Cuts at: 3147 3394 3410 5169 6127 442 Size: 247 16 1759 958 1753 Fragments arranged by size: 1759 1753 1225 958 497 416 399 247 83 53 24 16 8 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 7438 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4279 4279 Size: 7438 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2730 3464 4472 6098 1239 Size: 1491 734 1008 1626 2579 Fragments arranged by size: 2579 1626 1491 1008 734 Bpu10I CC'TnA_GC Cuts at: 4164 4191 4164 Size: 27 7411 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3034 5563 6428 6726 3034 Size: 2529 865 298 3746 Fragments arranged by size: 3746 2529 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 6101 882 Size: 617 4602 2219 Fragments arranged by size: 4602 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2278 2550 2956 4735 5229 493 Size: 711 1074 272 406 1779 494 2702 Fragments arranged by size: 2702 1779 1074 711 494 406 272 BsaBI GATnn'nnATC Cuts at: 7432 7432 Size: 7438 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 5245 5627 6262 275 Size: 53 83 186 936 3712 382 635 1451 Fragments arranged by size: 3712 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 5871 6269 6543 6690 7101 5871 Size: 398 274 147 411 6208 Fragments arranged by size: 6208 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2703 2733 4205 4235 1178 Size: 30 165 30 1300 30 1472 30 4381 Fragments arranged by size: 4381 1472 1300 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 7438 BseYI C'CCAG_C Cuts at: 2529 2606 2969 3078 4158 4645 6641 7137 2529 Size: 77 363 109 1080 487 1996 496 2830 Fragments arranged by size: 2830 1996 1080 496 487 363 109 77 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2297 1163 Size: 573 561 6304 Fragments arranged by size: 6304 573 561 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 4888 5649 6677 1540 Size: 275 186 2887 761 1028 2301 Fragments arranged by size: 2887 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 5499 5584 6655 5499 Size: 85 1071 6282 Fragments arranged by size: 6282 1071 85 BsmI GAATG_Cn' Cuts at: 4949 5042 4949 Size: 93 7345 BsmBI CGTCTCn'nnnn_ Cuts at: 3327 3327 Size: 7438 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2994 3392 3441 4372 4459 4590 4757 Size: 399 1204 398 49 931 87 131 167 Cuts at: 4757 5499 5584 6655 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1204 1071 931 742 399 398 167 131 87 85 49 BspEI T'CCGG_A Cuts at: 7101 7101 Size: 7438 BspHI T'CATG_A Cuts at: 5327 7057 5327 Size: 1730 5708 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2374 2668 4793 844 Size: 1530 294 2125 3489 Fragments arranged by size: 3489 2125 1530 294 BsrBI CCG'CTC Cuts at: 1394 1823 5325 1394 Size: 429 3502 3507 Fragments arranged by size: 3507 3502 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2177 4309 5933 6107 65 Size: 1183 929 2132 1624 174 1396 Fragments arranged by size: 2132 1624 1396 1183 929 174 BsrFI r'CCGG_y Cuts at: 1700 1810 6082 6269 1700 Size: 110 4272 187 2869 Fragments arranged by size: 4272 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 3690 96 Size: 3594 3844 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 7438 BssSI C'ACGA_G Cuts at: 5498 6510 5498 Size: 1012 6426 BstAPI GCAn_nnn'nTGC Cuts at: 794 3042 4499 794 Size: 2248 1457 3733 Fragments arranged by size: 3733 2248 1457 BstBI TT'CG_AA Cuts at: 2007 6242 2007 Size: 4235 3203 BstEII G'GTnAC_C Cuts at: 1184 4237 1184 Size: 3053 4385 BstXI CCAn_nnnn'nTGG Cuts at: 2466 2840 2466 Size: 374 7064 BstYI r'GATC_y Cuts at: 1972 3874 4416 4768 5135 5520 5537 6989 7075 Size: 1902 542 352 367 385 17 1452 86 Cuts at: 7075 7087 7433 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1902 1452 542 385 367 352 346 86 17 12 BstZ17I GTA'TAC Cuts at: 2175 2175 Size: 7438 Bsu36I CC'TnA_GG Cuts at: 4530 4530 Size: 7438 BtgI C'CryG_G Cuts at: 513 1065 2022 2151 2397 2895 3510 513 Size: 552 957 129 246 498 615 4441 Fragments arranged by size: 4441 957 615 552 498 246 129 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3457 487 Size: 683 56 2231 4468 Fragments arranged by size: 4468 2231 683 56 BtsI GCAGTG_nn' Cuts at: 3409 3666 4954 5748 5768 3409 Size: 257 1288 794 20 5079 Fragments arranged by size: 5079 1288 794 257 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 7438 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 4088 4123 4448 4483 529 Size: 35 3524 35 325 35 3484 Fragments arranged by size: 3524 3484 325 35 35 35 DraI TTT'AAA Cuts at: 4742 4742 Size: 7438 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 6445 809 Size: 507 5129 1802 Fragments arranged by size: 5129 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2922 2958 3105 Size: 54 1425 50 275 18 1092 36 147 Cuts at: 3105 3507 3663 4885 5774 7403 8 Size: 402 156 1222 889 1629 43 Fragments arranged by size: 1629 1425 1222 1092 889 402 275 156 147 54 50 43 36 18 EagI C'GGCC_G Cuts at: 1537 1812 4885 1537 Size: 275 3073 4090 Fragments arranged by size: 4090 3073 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2067 3314 5368 1353 Size: 714 1247 2054 3423 Fragments arranged by size: 3423 2054 1247 714 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3824 6025 6399 6545 387 Size: 1064 138 2235 2201 374 146 1280 Fragments arranged by size: 2235 2201 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2730 2952 3023 3228 3464 3501 3598 Size: 393 1098 222 71 205 236 37 97 Cuts at: 3598 4472 5501 6098 6885 7153 1239 Size: 874 1029 597 787 268 1524 Fragments arranged by size: 1524 1098 1029 874 787 597 393 268 236 222 205 97 71 37 EcoNI CCTnn'n_nnAGG Cuts at: 3541 3818 3541 Size: 277 7161 EcoO109I rG'GnC_Cy Cuts at: 2629 2644 3088 3497 2629 Size: 15 444 409 6570 Fragments arranged by size: 6570 444 409 15 EcoRI G'AATT_C Cuts at: 3147 4270 4747 5125 3147 Size: 1123 477 378 5460 Fragments arranged by size: 5460 1123 477 378 EcoRV GAT'ATC Cuts at: 4048 4048 Size: 7438 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3028 4066 4702 3028 Size: 1038 636 5764 Fragments arranged by size: 5764 1038 636 FspI TGC'GCA Cuts at: 5944 5944 Size: 7438 HaeII r_GCGC'y Cuts at: 1678 4436 6585 1678 Size: 2758 2149 2531 Fragments arranged by size: 2758 2531 2149 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2566 2598 4659 4691 6079 Size: 32 135 32 994 32 2061 32 1388 Cuts at: 6079 6111 6153 6185 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2061 1388 994 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2638 4782 5028 669 Size: 988 281 700 2144 246 3079 Fragments arranged by size: 3079 2144 988 700 281 246 HindIII A'AGCT_T Cuts at: 748 4566 748 Size: 3818 3620 HpaI GTT'AAC Cuts at: 2638 5028 2638 Size: 2390 5048 KpnI G_GTAC'C Cuts at: 4763 4763 Size: 7438 MfeI C'AATT_G Cuts at: 5037 5037 Size: 7438 MluI A'CGCG_T Cuts at: 2784 7314 2784 Size: 4530 2908 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2447 6552 6736 1305 Size: 1142 4105 184 2007 Fragments arranged by size: 4105 2007 1142 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2960 3107 3509 10 Size: 54 1425 343 1128 147 402 3939 Fragments arranged by size: 3939 1425 1128 402 343 147 54 MslI CAynn'nnrTG Cuts at: 518 1166 2328 3269 3434 3659 5396 5755 5914 Size: 648 1162 941 165 225 1737 359 159 Cuts at: 5914 518 Size: 2042 Fragments arranged by size: 2042 1737 1162 941 648 359 225 165 159 MspA1I CmG'CkG Cuts at: 1606 2529 3379 4168 4288 4854 5531 6679 6924 Size: 923 850 789 120 566 677 1148 245 Cuts at: 6924 1606 Size: 2120 Fragments arranged by size: 2120 1148 923 850 789 677 566 245 120 NcoI C'CATG_G Cuts at: 513 1065 2022 2397 3510 513 Size: 552 957 375 1113 4441 Fragments arranged by size: 4441 1113 957 552 375 NdeI CA'TA_TG Cuts at: 387 387 Size: 7438 NheI G'CTAG_C Cuts at: 1052 1052 Size: 7438 NotI GC'GGCC_GC Cuts at: 4885 4885 Size: 7438 NspI r_CATG'y Cuts at: 1132 1229 2827 3073 3713 4796 6341 1132 Size: 97 1598 246 640 1083 1545 2229 Fragments arranged by size: 2229 1598 1545 1083 640 246 97 PasI CC'CwG_GG Cuts at: 1348 3190 4079 1348 Size: 1842 889 4707 Fragments arranged by size: 4707 1842 889 PciI A'CATG_T Cuts at: 6337 6337 Size: 7438 PflMI CCAn_nnn'nTGG Cuts at: 1108 2403 3190 3422 1108 Size: 1295 787 232 5124 Fragments arranged by size: 5124 1295 787 232 PfoI T'CCnGG_A Cuts at: 2122 2122 Size: 7438 PmeI GTTT'AAAC Cuts at: 4742 4742 Size: 7438 PmlI CAC'GTG Cuts at: 2956 2956 Size: 7438 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 5507 5539 7020 7052 5507 Size: 32 1481 32 5893 Fragments arranged by size: 5893 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2644 3088 2644 Size: 444 6994 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 7438 PsiI TTA'TAA Cuts at: 5008 5008 Size: 7438 PstI C_TGCA'G Cuts at: 830 4524 4790 830 Size: 3694 266 3478 Fragments arranged by size: 3694 3478 266 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 7438 PvuII CAG'CTG Cuts at: 2529 4168 4288 2529 Size: 1639 120 5679 Fragments arranged by size: 5679 1639 120 SalI G'TCGA_C Cuts at: 1936 4780 1936 Size: 2844 4594 SanDI GG'GwC_CC Cuts at: 2644 2644 Size: 7438 SbfI CC_TGCA'GG Cuts at: 4790 4790 Size: 7438 ScaI AGT'ACT Cuts at: 3157 5686 7398 3157 Size: 2529 1712 3197 Fragments arranged by size: 3197 2529 1712 SfcI C'TryA_G Cuts at: 826 1045 2143 3857 4520 4786 5921 6602 6793 Size: 219 1098 1714 663 266 1135 681 191 Cuts at: 6793 826 Size: 1471 Fragments arranged by size: 1714 1471 1135 1098 681 663 266 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 7438 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 7438 SmaI CCC'GGG Cuts at: 1872 3314 4604 4765 1872 Size: 1442 1290 161 4545 Fragments arranged by size: 4545 1442 1290 161 SmlI C'TyrA_G Cuts at: 820 1017 1943 3013 5542 6443 6705 820 Size: 197 926 1070 2529 901 262 1553 Fragments arranged by size: 2529 1553 1070 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4735 5229 493 Size: 4242 494 2702 Fragments arranged by size: 4242 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 7438 SphI G_CATG'C Cuts at: 1132 2827 4796 1132 Size: 1695 1969 3774 Fragments arranged by size: 3774 1969 1695 SspI AAT'ATT Cuts at: 5 52 2482 5362 5 Size: 47 2430 2880 2081 Fragments arranged by size: 2880 2430 2081 47 StuI AGG'CCT Cuts at: 1517 2234 3813 4428 1517 Size: 717 1579 615 4527 Fragments arranged by size: 4527 1579 717 615 StyI C'CwwG_G Cuts at: 513 1065 2022 2397 3510 3542 3808 4877 513 Size: 552 957 375 1113 32 266 1069 3074 Fragments arranged by size: 3074 1113 1069 957 552 375 266 32 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3317 3514 4259 4637 5465 5482 5635 Size: 1255 1737 197 745 378 828 17 153 Cuts at: 5635 5820 6265 325 Size: 185 445 1498 Fragments arranged by size: 1737 1498 1255 828 745 445 378 197 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3155 3690 4212 Size: 274 80 33 51 1443 1178 535 522 Cuts at: 4212 5684 7396 96 Size: 1472 1712 138 Fragments arranged by size: 1712 1472 1443 1178 535 522 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2703 2735 4037 4069 4349 4381 4438 4470 7398 Size: 32 1302 32 280 32 57 32 2928 Cuts at: 7398 7430 2703 Size: 32 2711 Fragments arranged by size: 2928 2711 1302 280 57 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 4616 1316 Size: 3300 4138 XbaI T'CTAG_A Cuts at: 4774 4774 Size: 7438 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2677 3115 2677 Size: 438 7000 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 7438 XmaI C'CCGG_G Cuts at: 1870 3312 4602 4763 1870 Size: 1442 1290 161 4545 Fragments arranged by size: 4545 1442 1290 161 XmnI GAAnn'nnTTC Cuts at: 4629 4685 5567 4629 Size: 56 882 6500 Fragments arranged by size: 6500 882 56 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 5246 276 Size: 53 83 186 936 3712 2468 Fragments arranged by size: 3712 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BstZ17I Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BbeI BfrBI BsiWI DraIII EcoICRI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI