(Circular) (Six-base) MAPSORT of: pFN21ASDA0017.seq Check: 5313 from: 1 to: 8380 pFN21ASDA0017 8380 bp With 182 enzymes: SgfI * January 16, 2009 15:29 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 5472 5472 Size: 8380 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6190 278 Size: 53 83 186 936 4654 2468 Fragments arranged by size: 4654 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3412 5723 1937 Size: 1475 2311 4594 Fragments arranged by size: 4594 2311 1475 Acc65I G'GTAC_C Cuts at: 4098 5701 4098 Size: 1603 6777 AclI AA'CG_TT Cuts at: 1563 6507 6880 1563 Size: 4944 373 3063 Fragments arranged by size: 4944 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3717 5625 6443 7827 8095 1632 Size: 2085 1908 818 1384 268 1917 Fragments arranged by size: 2085 1917 1908 1384 818 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8183 AflIII A'CryG_T Cuts at: 1203 7279 8256 1203 Size: 6076 977 1327 Fragments arranged by size: 6076 1327 977 AgeI A'CCGG_T Cuts at: 7211 7211 Size: 8380 AhdI GACnn_n'nnGTC Cuts at: 3408 7109 3408 Size: 3701 4679 AleI CACnn'nnGTG Cuts at: 1166 2519 3470 1166 Size: 1353 951 6076 Fragments arranged by size: 6076 1353 951 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 5426 1184 Size: 4242 4138 AlwNI CAG_nnn'CTG Cuts at: 2738 6123 7695 2738 Size: 3385 1572 3423 Fragments arranged by size: 3423 3385 1572 ApaLI G'TGCA_C Cuts at: 6437 7593 6437 Size: 1156 7224 ApoI r'AATT_y Cuts at: 1493 3077 4241 4382 5689 5915 6067 8176 1493 Size: 1584 1164 141 1307 226 152 2109 1697 Fragments arranged by size: 2109 1697 1584 1307 1164 226 152 141 AseI AT'TA_AT Cuts at: 160 1032 6934 160 Size: 872 5902 1606 Fragments arranged by size: 5902 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8380 AvaI C'yCGr_G Cuts at: 1870 1943 4274 5645 5705 1870 Size: 73 2331 1371 60 4545 Fragments arranged by size: 4545 2331 1371 73 60 BamHI G'GATC_C Cuts at: 2392 3727 3880 4998 5227 5710 6077 2392 Size: 1335 153 1118 229 483 367 4695 Fragments arranged by size: 4695 1335 1118 483 367 229 153 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2240 4098 5701 7156 618 Size: 325 217 627 453 1858 1603 1455 1842 Fragments arranged by size: 1858 1842 1603 1455 627 453 325 217 BanII G_rGCy'C Cuts at: 1391 3998 4585 5699 1391 Size: 2607 587 1114 4072 Fragments arranged by size: 4072 2607 1114 587 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3638 4333 4731 5345 928 Size: 971 1739 695 398 614 3963 Fragments arranged by size: 3963 1739 971 695 614 398 BbvCI CC'TCA_GC Cuts at: 4046 5504 4046 Size: 1458 6922 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3809 3843 6571 6605 1903 Size: 34 1872 34 2728 34 3678 Fragments arranged by size: 3678 2728 1872 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6272 7482 6272 Size: 1210 7170 BclI T'GATC_A Cuts at: 1548 5295 1548 Size: 3747 4633 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 6991 136 Size: 107 122 71 631 605 5319 1525 Fragments arranged by size: 5319 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2130 8375 2130 Size: 6245 2135 BlpI GC'TnA_GC Cuts at: 5797 5797 Size: 8380 Bme1580I G_kGCm'C Cuts at: 1790 6441 7597 1790 Size: 4651 1156 2573 Fragments arranged by size: 4651 2573 1156 BmgBI CAC'GTC Cuts at: 1318 5421 1318 Size: 4103 4277 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4080 6111 7069 Size: 416 53 83 399 24 2663 2031 958 Cuts at: 7069 442 Size: 1753 Fragments arranged by size: 2663 2031 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8380 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4012 4012 Size: 8380 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2760 3834 4853 5376 5412 7040 1239 Size: 1521 1074 1019 523 36 1628 2579 Fragments arranged by size: 2579 1628 1521 1074 1019 523 36 Bpu10I CC'TnA_GC Cuts at: 4046 5504 4046 Size: 1458 6922 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3368 6505 7370 7668 3368 Size: 3137 865 298 4080 Fragments arranged by size: 4080 3137 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3831 5544 7043 882 Size: 617 2332 1713 1499 2219 Fragments arranged by size: 2332 2219 1713 1499 617 BsaAI yAC'GTr Cuts at: 493 1204 5677 6171 493 Size: 711 4473 494 2702 Fragments arranged by size: 4473 2702 711 494 BsaBI GATnn'nnATC Cuts at: 5226 8374 5226 Size: 3148 5232 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 6187 6569 7204 275 Size: 53 83 186 936 4654 382 635 1451 Fragments arranged by size: 4654 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2208 5655 6813 7211 7485 7632 8043 2208 Size: 3447 1158 398 274 147 411 2545 Fragments arranged by size: 3447 2545 1158 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2529 2559 3477 3507 3696 Size: 30 165 30 1126 30 918 30 189 Cuts at: 3696 3726 1178 Size: 30 5832 Fragments arranged by size: 5832 1126 918 189 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3717 1186 Size: 2531 5849 BseYI C'CCAG_C Cuts at: 2634 3922 3927 3982 5156 7583 8079 2634 Size: 1288 5 55 1174 2427 496 2935 Fragments arranged by size: 2935 2427 1288 1174 496 55 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3593 3684 4723 1163 Size: 573 1857 91 1039 4820 Fragments arranged by size: 4820 1857 1039 573 91 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 5830 6591 7619 1540 Size: 275 186 3829 761 1028 2301 Fragments arranged by size: 3829 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 4585 6441 6526 7597 4585 Size: 1856 85 1071 5368 Fragments arranged by size: 5368 1856 1071 85 BsmI GAATG_Cn' Cuts at: 3466 3591 4143 4544 5891 5984 3466 Size: 125 552 401 1347 93 5862 Fragments arranged by size: 5862 1347 552 401 125 93 BsmBI CGTCTCn'nnnn_ Cuts at: 5312 5605 5312 Size: 293 8087 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3998 4585 5699 6441 6526 7597 1391 Size: 399 2208 587 1114 742 85 1071 2174 Fragments arranged by size: 2208 2174 1114 1071 742 587 399 85 BspEI T'CCGG_A Cuts at: 5655 8043 5655 Size: 2388 5992 BspHI T'CATG_A Cuts at: 5298 6269 7999 5298 Size: 971 1730 5679 Fragments arranged by size: 5679 1730 971 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3565 4695 5472 5735 844 Size: 2721 1130 777 263 3489 Fragments arranged by size: 3489 2721 1130 777 263 BsrBI CCG'CTC Cuts at: 1394 1823 4176 4516 6267 1394 Size: 429 2353 340 1751 3507 Fragments arranged by size: 3507 2353 1751 429 340 BsrDI GCAATG_nn' Cuts at: 65 1248 4603 6875 7049 65 Size: 1183 3355 2272 174 1396 Fragments arranged by size: 3355 2272 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 7024 7211 1700 Size: 110 5214 187 2869 Fragments arranged by size: 5214 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2031 96 Size: 1935 6445 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8380 BssSI C'ACGA_G Cuts at: 6440 7452 6440 Size: 1012 7368 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 8380 BstBI TT'CG_AA Cuts at: 2007 7184 2007 Size: 5177 3203 BstEII G'GTnAC_C Cuts at: 1184 2807 3383 1184 Size: 1623 576 6181 Fragments arranged by size: 6181 1623 576 BstXI CCAn_nnnn'nTGG Cuts at: 3157 5009 5137 3157 Size: 1852 128 6400 Fragments arranged by size: 6400 1852 128 BstYI r'GATC_y Cuts at: 1972 2130 2392 2994 3727 3880 4998 5227 5710 Size: 158 262 602 733 153 1118 229 483 Cuts at: 5710 6077 6462 6479 7931 8017 8029 8375 1972 Size: 367 385 17 1452 86 12 346 1977 Fragments arranged by size: 1977 1452 1118 733 602 483 385 367 346 262 229 158 153 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2122 5257 5574 513 Size: 552 1057 3135 317 3319 Fragments arranged by size: 3319 3135 1057 552 317 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 7641 Fragments arranged by size: 7641 683 56 BtsI GCAGTG_nn' Cuts at: 2272 3672 4361 4610 5896 6690 6710 2272 Size: 1400 689 249 1286 794 20 3942 Fragments arranged by size: 3942 1400 1286 794 689 249 20 ClaI AT'CG_AT Cuts at: 1554 2912 1554 Size: 1358 7022 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 8345 DraI TTT'AAA Cuts at: 2673 5684 2673 Size: 3011 5369 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3617 3860 5326 7387 809 Size: 507 2301 243 1466 2061 1802 Fragments arranged by size: 2301 2061 1802 1466 507 243 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2374 3267 3798 Size: 54 1425 50 275 18 544 893 531 Cuts at: 3798 4161 4287 4524 4749 5827 6716 8345 8 Size: 363 126 237 225 1078 889 1629 43 Fragments arranged by size: 1629 1425 1078 893 889 544 531 363 275 237 225 126 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 5827 1537 Size: 275 4015 4090 Fragments arranged by size: 4090 4015 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4130 4854 6310 1353 Size: 2777 724 1456 3423 Fragments arranged by size: 3423 2777 1456 724 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2860 6967 7341 7487 387 Size: 1064 138 1271 4107 374 146 1280 Fragments arranged by size: 4107 1280 1271 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 4583 4583 Size: 8380 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2760 3717 3834 4853 5376 5412 5625 Size: 393 1128 957 117 1019 523 36 213 Cuts at: 5625 6443 7040 7827 8095 1239 Size: 818 597 787 268 1524 Fragments arranged by size: 1524 1128 1019 957 818 787 597 523 393 268 213 117 36 EcoNI CCTnn'n_nnAGG Cuts at: 2868 3304 2868 Size: 436 7944 EcoO109I rG'GnC_Cy Cuts at: 3311 4107 4756 4864 5274 3311 Size: 796 649 108 410 6417 Fragments arranged by size: 6417 796 649 410 108 EcoRI G'AATT_C Cuts at: 5689 6067 5689 Size: 378 8002 EcoRV GAT'ATC Cuts at: 2821 2950 5653 2821 Size: 129 2703 5548 Fragments arranged by size: 5548 2703 129 FspI TGC'GCA Cuts at: 6886 6886 Size: 8380 HaeII r_GCGC'y Cuts at: 1678 7527 1678 Size: 5849 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2529 2561 2754 2786 3128 Size: 32 135 32 957 32 193 32 342 Cuts at: 3128 3160 3218 3247 3250 3279 3394 3426 3696 Size: 32 58 29 3 29 115 32 270 Cuts at: 3696 3728 3809 3841 7021 7053 7095 7127 1373 Size: 32 81 32 3180 32 42 32 2626 Fragments arranged by size: 3180 2626 957 342 270 193 135 115 81 58 42 32 32 32 32 32 32 32 32 32 32 29 29 3 HincII GTy'rAC Cuts at: 669 1657 1938 3413 5724 5970 669 Size: 988 281 1475 2311 246 3079 Fragments arranged by size: 3079 2311 1475 988 281 246 HindIII A'AGCT_T Cuts at: 748 4808 748 Size: 4060 4320 HpaI GTT'AAC Cuts at: 5970 5970 Size: 8380 KpnI G_GTAC'C Cuts at: 4102 5705 4102 Size: 1603 6777 MfeI C'AATT_G Cuts at: 3651 5979 3651 Size: 2328 6052 MluI A'CGCG_T Cuts at: 8256 8256 Size: 8380 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2515 2527 2633 3204 4737 7494 7678 1305 Size: 1210 12 106 571 1533 2757 184 2007 Fragments arranged by size: 2757 2007 1533 1210 571 184 106 12 MscI TGG'CCA Cuts at: 10 64 1489 1832 2376 3269 3800 4163 4289 Size: 54 1425 343 544 893 531 363 126 Cuts at: 4289 4751 10 Size: 462 3639 Fragments arranged by size: 3639 1425 893 544 531 462 363 343 126 54 MslI CAynn'nnrTG Cuts at: 518 1166 2519 3041 3446 3470 5183 5408 6338 Size: 648 1353 522 405 24 1713 225 930 Cuts at: 6338 6697 6856 518 Size: 359 159 2042 Fragments arranged by size: 2042 1713 1353 930 648 522 405 359 225 159 24 MspA1I CmG'CkG Cuts at: 1606 2826 3742 3823 4666 5796 6473 7621 7866 Size: 1220 916 81 843 1130 677 1148 245 Cuts at: 7866 1606 Size: 2120 Fragments arranged by size: 2120 1220 1148 1130 916 843 677 245 81 NcoI C'CATG_G Cuts at: 513 1065 5574 513 Size: 552 4509 3319 Fragments arranged by size: 4509 3319 552 NdeI CA'TA_TG Cuts at: 387 5180 387 Size: 4793 3587 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8380 NotI GC'GGCC_GC Cuts at: 5827 5827 Size: 8380 NspI r_CATG'y Cuts at: 1132 1229 3913 5453 5738 7283 1132 Size: 97 2684 1540 285 1545 2229 Fragments arranged by size: 2684 2229 1545 1540 285 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 8380 PciI A'CATG_T Cuts at: 7279 7279 Size: 8380 PflMI CCAn_nnn'nTGG Cuts at: 1108 5452 1108 Size: 4344 4036 PfoI T'CCnGG_A Cuts at: 2737 5136 2737 Size: 2399 5981 PmeI GTTT'AAAC Cuts at: 5684 5684 Size: 8380 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 6449 6481 7962 7994 6449 Size: 32 1481 32 6835 Fragments arranged by size: 6835 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3311 4107 4756 4864 3311 Size: 796 649 108 6827 Fragments arranged by size: 6827 796 649 108 PshAI GACnn'nnGTC Cuts at: 1613 2274 1613 Size: 661 7719 PsiI TTA'TAA Cuts at: 5950 5950 Size: 8380 PspXI vC'TCGA_Gb Cuts at: 5645 5645 Size: 8380 PstI C_TGCA'G Cuts at: 830 2470 4368 5732 830 Size: 1640 1898 1364 3478 Fragments arranged by size: 3478 1898 1640 1364 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8380 PvuII CAG'CTG Cuts at: 3742 4666 3742 Size: 924 7456 SacI G_AGCT'C Cuts at: 4585 4585 Size: 8380 SalI G'TCGA_C Cuts at: 1936 3411 5722 1936 Size: 1475 2311 4594 Fragments arranged by size: 4594 2311 1475 SanDI GG'GwC_CC Cuts at: 3311 4107 3311 Size: 796 7584 SapI GCTCTTCn'nnn_ Cuts at: 4130 4130 Size: 8380 SbfI CC_TGCA'GG Cuts at: 5732 5732 Size: 8380 ScaI AGT'ACT Cuts at: 2766 6628 8340 2766 Size: 3862 1712 2806 Fragments arranged by size: 3862 2806 1712 SexAI A'CCwGG_T Cuts at: 3379 3379 Size: 8380 SfcI C'TryA_G Cuts at: 826 1045 2466 3492 4028 4364 5124 5232 5728 Size: 219 1421 1026 536 336 760 108 496 Cuts at: 5728 6863 7544 7735 826 Size: 1135 681 191 1471 Fragments arranged by size: 1471 1421 1135 1026 760 681 536 496 336 219 191 108 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8380 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8380 SmaI CCC'GGG Cuts at: 1872 5707 1872 Size: 3835 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3347 5645 6484 7385 7647 820 Size: 197 926 1404 2298 839 901 262 1553 Fragments arranged by size: 2298 1553 1404 926 901 839 262 197 SnaBI TAC'GTA Cuts at: 493 5677 6171 493 Size: 5184 494 2702 Fragments arranged by size: 5184 2702 494 SpeI A'CTAG_T Cuts at: 152 152 Size: 8380 SphI G_CATG'C Cuts at: 1132 3913 5738 1132 Size: 2781 1825 3774 Fragments arranged by size: 3774 2781 1825 SspI AAT'ATT Cuts at: 5 52 3008 4021 6304 5 Size: 47 2956 1013 2283 2081 Fragments arranged by size: 2956 2283 2081 1013 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 8380 StyI C'CwwG_G Cuts at: 513 1065 2665 3567 4587 4752 5574 5819 513 Size: 552 1600 902 1020 165 822 245 3074 Fragments arranged by size: 3074 1600 1020 902 822 552 245 165 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3947 3974 4390 4733 5631 6407 6424 Size: 1255 2367 27 416 343 898 776 17 Cuts at: 6424 6577 6762 7207 325 Size: 153 185 445 1498 Fragments arranged by size: 2367 1498 1255 898 776 445 416 343 185 153 27 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2031 2170 2764 Size: 274 80 33 51 1443 54 139 594 Cuts at: 2764 3750 4368 6626 8338 96 Size: 986 618 2258 1712 138 Fragments arranged by size: 2258 1712 1443 986 618 594 274 139 138 80 54 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3336 3368 8340 8372 3336 Size: 32 4972 32 3344 Fragments arranged by size: 4972 3344 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3538 4064 4658 1316 Size: 2222 526 594 5038 Fragments arranged by size: 5038 2222 594 526 XbaI T'CTAG_A Cuts at: 5716 5716 Size: 8380 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2339 2339 Size: 8380 XhoI C'TCGA_G Cuts at: 1943 5645 1943 Size: 3702 4678 XmaI C'CCGG_G Cuts at: 1870 5705 1870 Size: 3835 4545 XmnI GAAnn'nnTTC Cuts at: 2086 4124 6509 2086 Size: 2038 2385 3957 Fragments arranged by size: 3957 2385 2038 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6188 276 Size: 53 83 186 936 4654 2468 Fragments arranged by size: 4654 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI PvuII SacI SalI SanDI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BsiWI BstZ17I Bsu36I DraIII FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PsrI RsrII SacII SfiI SfoI SrfI SwaI