Restriction map for
pFN21AB9402
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# NO CUT ENZYMES ( 118 enzymes) #
##################################
AatII Acc65I AccIII AcsI AflII
AflIII AgeI AhdI AocI Aor51HI
ApoI AscI AseI AsnI Asp700I
Asp718I AspEI AviII AvrII BalI
BamHI BbeI BbrPI BcgI BciVI
BclI BfrI BglII BlnI BlpI
Bpu1102I BsaI BseAI BsiEI BsiWI
BsmI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrDI BsrGI BssHII Bst1107I
BstAPI BstBI BstZ17I Bsu36I CelII
ClaI CpoI DraI DrdI EagI
Eam1105I EclXI Eco47III Eco52I Eco81I
EcoRI EcoRV EcoT22I FbaI FseI
FspI HindIII HpaI KasI KpnI
MfeI MluI MluNI MroI MscI
MseI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PinAI
PmaCI PmeI PmlI PshAI PshBI
PvuI RcaI RsrII SacI SapI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
Sse8387I SspBI SspI SwaI Tru9I
XbaI XhoI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AclI 643 AACGTT AA^CGTT
AcyI 36 GGCGTC G[GA]^CG[CT]C
ApaI 171 GGGCCC GGGCC^C
AspI 1444 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 1582 GGTGCC G^G[CT][GA]CC
BbvCI 904 CCTCAGC CCTCAGC(-5/-2)
BglI 824 GCCGGGAGGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 141 TACGTG [CT]AC^GT[GA]
BsaBI 81 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 36 GGCGTC G[GA]^CG[CT]C
BsaWI 624 TCCGGT [AT]^CCGG[AT]
BsgI 932 CTGCAC GTGCAG(16/14)
BsmBI 1442 GAGACG CGTCTC(1/5)
BsrBI 505 GAGCGG CCGCTC(-3/-3)
BssSI 634 CACGAG CACGAG(-5/-1)
BstXI 1160 CCAAGAGTGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
EcoT14I 176 CCAAGG C^C[AT][AT]GG
Hin1I 36 GGCGTC G[GA]^CG[CT]C
HincII 895 GTCGAC GT[CT]^[GA]AC
HindII 895 GTCGAC GT[CT]^[GA]AC
KspI 604 CCGCGG CCGC^GG
MamI 81 GATCAAGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
NspI 419 GCATGC [GA]CATG^[CT]
PflFI 1444 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 137 CCACTACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 183 GACTC GAGTC(4/5)
PpuMI 397 AGGTCCC [GA]G^G[AT]CC[CT]
Psp1406I 643 AACGTT AA^CGTT
PvuII 521 CAGCTG CAG^CTG
SacII 604 CCGCGG CCGC^GG
SalI 895 GTCGAC G^TCGAC
SmaI 209 CCCGGG CCC^GGG
SphI 419 GCATGC GCATG^C
StuI 901 AGGCCT AGG^CCT
StyI 176 CCAAGG C^C[AT][AT]GG
Tsp509I 1014 AATT ^AATT
Tth111I 1444 GACGGTGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 137 CCACTACGTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 209 CCCGGG C^CCGGG
XmaI 209 CCCGGG C^CCGGG
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# TWO CUT ENZYMES ( 25 enzymes) #
###################################
AccI 895 GTCGAC GT^[AC][GT]AC
1315 GTAGAC
Alw44I 322 GTGCAC G^TGCAC
883 GTGCAC
AlwNI 1297 CAGGGGCTG CAG[ACGT][ACGT][ACGT]^CTG
1555 CAGCCACTG
ApaLI 322 GTGCAC G^TGCAC
883 GTGCAC
AvaI 209 CCCGGG C^[CT]CG[GA]G
1408 CCCGAG
BbsI 687 GAAGAC GAAGAC(2/6)
1335 GAAGAC
BfaI 221 CTAG C^TAG
236 CTAG
BpuAI 687 GAAGAC GAAGAC(2/6)
1335 GAAGAC
BsoBI 209 CCCGGG C^[CT]CG[GA]G
1408 CCCGAG
BspMI 30 GCAGGT ACCTGC(4/8)
318 GCAGGT
BsrFI 100 ACCGGC [GA]^CCGG[CT]
314 ACCGGC
BstEII 49 GGTGACC G^GT[ACGT]ACC
1708 GGTAACC
BstPI 49 GGTGACC G^GT[ACGT]ACC
1708 GGTAACC
BstYI 746 AGATCC [GA]^GATC[CT]
1629 AGATCC
Cfr10I 100 ACCGGC [GA]^CCGG[CT]
314 ACCGGC
EaeI 554 CGGCCA [CT]^GGCC[GA]
652 CGGCCA
EcoNI 569 CCTCCGGCAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
1284 CCTGGAGAAGG
EcoO65I 49 GGTGACC G^GT[ACGT]ACC
1708 GGTAACC
HaeII 4 AGCGCC [GA]GCGC^[CT]
304 GGCGCT
MaeI 221 CTAG C^TAG
236 CTAG
MaeII 142 ACGT A^CGT
644 ACGT
MflI 746 AGATCC [GA]^GATC[CT]
1629 AGATCC
PstI 1205 CTGCAG CTGCA^G
1561 CTGCAG
XcmI 23 CCATGATGCAGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
1376 CCAAAGAGCTGCTGG
XhoII 746 AGATCC [GA]^GATC[CT]
1629 AGATCC
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# 3 OR MORE CUT ENZYMES ( 75 enzymes) #
#########################################
AccII AciI AfaI AluI AlwI
AspHI AvaII BanII BbvI BcnI
BmyI BpmI BsaJI BseRI BsiHKAI
BsiYI BslI BsmAI BsmFI Bsp1286I
BsrI BssKI BstF5I BstNI BstUI
Cac8I CfoI Cfr13I DdeI DpnII
DraII DraIII DsaI EarI EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HgaI HhaI HinP1I HinfI
HpaII HphI ItaI Ksp632I MaeIII
MboI MboII MnlI MslI MspA1I
MspI MvaI MvnI MwoI NciI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI TaqI
TfiI TseI Tsp45I TspRI TthHB8I