(Circular) (Six-base) MAPSORT of: pFN21AB9402.seq Check: 2677 from: 1 to: 6430 pFN21AB9402 6430 bp With 182 enzymes: SgfI * January 16, 2009 15:28 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2026 2314 2026 Size: 288 6142 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4240 278 Size: 53 83 186 936 2704 2468 Fragments arranged by size: 2704 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2901 3321 3773 1937 Size: 964 420 452 4594 Fragments arranged by size: 4594 964 452 420 Acc65I G'GTAC_C Cuts at: 3751 3751 Size: 6430 AclI AA'CG_TT Cuts at: 1563 2649 4557 4930 1563 Size: 1086 1908 373 3063 Fragments arranged by size: 3063 1908 1086 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4493 5877 6145 1632 Size: 2861 1384 268 1917 Fragments arranged by size: 2861 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6233 AflIII A'CryG_T Cuts at: 1203 5329 6306 1203 Size: 4126 977 1327 Fragments arranged by size: 4126 1327 977 AgeI A'CCGG_T Cuts at: 3727 5261 3727 Size: 1534 4896 AhdI GACnn_n'nnGTC Cuts at: 5159 5159 Size: 6430 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6430 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6430 AlwNI CAG_nnn'CTG Cuts at: 3307 3565 4173 5745 3307 Size: 258 608 1572 3992 Fragments arranged by size: 3992 1572 608 258 ApaI G_GGCC'C Cuts at: 2180 2180 Size: 6430 ApaLI G'TGCA_C Cuts at: 2327 2888 4487 5643 2327 Size: 561 1599 1156 3114 Fragments arranged by size: 3114 1599 1156 561 ApoI r'AATT_y Cuts at: 1493 3739 3965 4117 6226 1493 Size: 2246 226 152 2109 1697 Fragments arranged by size: 2246 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4984 160 Size: 872 3952 1606 Fragments arranged by size: 3952 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6430 AvaI C'yCGr_G Cuts at: 1870 1943 2214 3413 3755 1870 Size: 73 271 1199 342 4545 Fragments arranged by size: 4545 1199 342 271 73 BamHI G'GATC_C Cuts at: 3760 4127 3760 Size: 367 6063 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3587 3751 5206 618 Size: 325 217 627 1800 164 1455 1842 Fragments arranged by size: 1842 1800 1455 627 325 217 164 BanII G_rGCy'C Cuts at: 1391 2180 2225 2775 2840 3749 1391 Size: 789 45 550 65 909 4072 Fragments arranged by size: 4072 909 789 550 65 45 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2699 3347 928 Size: 971 800 648 4011 Fragments arranged by size: 4011 971 800 648 BbvCI CC'TCA_GC Cuts at: 2910 2910 Size: 6430 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4621 4655 1903 Size: 34 2684 34 3678 Fragments arranged by size: 3678 2684 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4322 5532 4322 Size: 1210 5220 BclI T'GATC_A Cuts at: 1548 1548 Size: 6430 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2835 5041 136 Size: 107 122 71 631 605 1163 2206 1525 Fragments arranged by size: 2206 1525 1163 631 605 122 107 71 BglII A'GATC_T Cuts at: 6425 6425 Size: 6430 BlpI GC'TnA_GC Cuts at: 3847 3847 Size: 6430 Bme1580I G_kGCm'C Cuts at: 1790 2169 2180 2331 2892 4491 5647 1790 Size: 379 11 151 561 1599 1156 2573 Fragments arranged by size: 2573 1599 1156 561 379 151 11 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6430 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2401 2512 2776 Size: 416 53 83 399 24 984 111 264 Cuts at: 2776 2978 4161 5119 442 Size: 202 1183 958 1753 Fragments arranged by size: 1753 1183 984 958 416 399 264 202 111 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6430 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2508 3096 3311 3329 3518 5090 1239 Size: 1269 588 215 18 189 1572 2579 Fragments arranged by size: 2579 1572 1269 588 215 189 18 Bpu10I CC'TnA_GC Cuts at: 2508 2910 2508 Size: 402 6028 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4555 5420 5718 4555 Size: 865 298 5267 Fragments arranged by size: 5267 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5093 882 Size: 617 3594 2219 Fragments arranged by size: 3594 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2148 4221 493 Size: 711 944 2073 2702 Fragments arranged by size: 2702 2073 944 711 BsaBI GATnn'nnATC Cuts at: 2090 6424 2090 Size: 4334 2096 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2042 4237 4619 5254 Size: 53 83 186 936 509 2195 382 635 Cuts at: 5254 275 Size: 1451 Fragments arranged by size: 2195 1451 936 635 509 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2629 3727 4863 5261 5535 5682 6093 2629 Size: 1098 1136 398 274 147 411 2966 Fragments arranged by size: 2966 1136 1098 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2982 3012 3436 3466 1178 Size: 30 165 30 1579 30 424 30 4142 Fragments arranged by size: 4142 1579 424 165 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2385 2545 3117 1186 Size: 1199 160 572 4499 Fragments arranged by size: 4499 1199 572 160 BseYI C'CCAG_C Cuts at: 2265 2349 2484 2491 2667 2704 2949 3131 3197 Size: 84 135 7 176 37 245 182 66 Cuts at: 3197 3539 3651 3689 3718 5633 6129 2265 Size: 342 112 38 29 1915 496 2566 Fragments arranged by size: 2566 1915 496 342 245 182 176 135 112 84 66 38 37 29 7 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2922 1163 Size: 573 1186 4671 Fragments arranged by size: 4671 1186 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3880 4641 5669 1540 Size: 275 186 1879 761 1028 2301 Fragments arranged by size: 2301 1879 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2301 2331 2892 4491 4576 5647 2301 Size: 30 561 1599 85 1071 3084 Fragments arranged by size: 3084 1599 1071 561 85 30 BsmI GAATG_Cn' Cuts at: 3941 4034 3941 Size: 93 6337 BsmBI CGTCTCn'nnnn_ Cuts at: 3441 3441 Size: 6430 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2169 2180 2225 2301 2331 2775 2840 Size: 399 379 11 45 76 30 444 65 Cuts at: 2840 2892 3749 4491 4576 5647 1391 Size: 52 857 742 85 1071 2174 Fragments arranged by size: 2174 1071 857 742 444 399 379 85 76 65 52 45 30 11 BspEI T'CCGG_A Cuts at: 6093 6093 Size: 6430 BspHI T'CATG_A Cuts at: 4319 6049 4319 Size: 1730 4700 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2026 2314 3785 844 Size: 1182 288 1471 3489 Fragments arranged by size: 3489 1471 1182 288 BsrBI CCG'CTC Cuts at: 1394 1823 2512 4317 1394 Size: 429 689 1805 3507 Fragments arranged by size: 3507 1805 689 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4925 5099 65 Size: 1183 3677 174 1396 Fragments arranged by size: 3677 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2105 2319 3727 5074 5261 1700 Size: 110 295 214 1408 1347 187 2869 Fragments arranged by size: 2869 1408 1347 295 214 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6430 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6430 BssSI C'ACGA_G Cuts at: 2639 4490 5502 2639 Size: 1851 1012 3567 Fragments arranged by size: 3567 1851 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6430 BstBI TT'CG_AA Cuts at: 5234 5234 Size: 6430 BstEII G'GTnAC_C Cuts at: 1184 2054 3713 1184 Size: 870 1659 3901 Fragments arranged by size: 3901 1659 870 BstXI CCAn_nnnn'nTGG Cuts at: 3172 3172 Size: 6430 BstYI r'GATC_y Cuts at: 1972 2751 3634 3760 4127 4512 4529 5981 6067 Size: 779 883 126 367 385 17 1452 86 Cuts at: 6067 6079 6425 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 883 779 385 367 346 126 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2500 2609 2895 3591 513 Size: 552 1435 109 286 696 3352 Fragments arranged by size: 3352 1435 696 552 286 109 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5691 Fragments arranged by size: 5691 683 56 BtsI GCAGTG_nn' Cuts at: 3201 3563 3946 4740 4760 3201 Size: 362 383 794 20 4871 Fragments arranged by size: 4871 794 383 362 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6430 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6395 DraI TTT'AAA Cuts at: 3734 3734 Size: 6430 DraIII CAC_nnn'GTG Cuts at: 2148 2257 2896 2148 Size: 109 639 5682 Fragments arranged by size: 5682 639 109 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5437 809 Size: 507 4121 1802 Fragments arranged by size: 4121 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2559 2657 3877 Size: 54 1425 50 275 18 729 98 1220 Cuts at: 3877 4766 6395 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1220 889 729 275 98 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3877 1537 Size: 275 2065 4090 Fragments arranged by size: 4090 2065 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3163 3177 3363 4360 1353 Size: 1810 14 186 997 3423 Fragments arranged by size: 3423 1810 997 186 14 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5017 5391 5537 387 Size: 1064 138 3428 374 146 1280 Fragments arranged by size: 3428 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2508 3096 3311 3329 3518 4493 5090 Size: 393 876 588 215 18 189 975 597 Cuts at: 5090 5877 6145 1239 Size: 787 268 1524 Fragments arranged by size: 1524 975 876 787 597 588 393 268 215 189 18 EcoNI CCTnn'n_nnAGG Cuts at: 2578 3293 2578 Size: 715 5715 EcoO109I rG'GnC_Cy Cuts at: 2176 2177 2403 2176 Size: 1 226 6203 Fragments arranged by size: 6203 226 1 EcoRI G'AATT_C Cuts at: 3739 4117 3739 Size: 378 6052 FspI TGC'GCA Cuts at: 4936 4936 Size: 6430 HaeII r_GCGC'y Cuts at: 1678 2013 2313 5577 1678 Size: 335 300 3264 2531 Fragments arranged by size: 3264 2531 335 300 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2070 2102 2177 2209 2528 Size: 32 135 32 498 32 75 32 319 Cuts at: 2528 2560 2982 3014 3438 3470 5071 5103 5145 Size: 32 422 32 424 32 1601 32 42 Cuts at: 5145 5177 1373 Size: 32 2626 Fragments arranged by size: 2626 1601 498 424 422 319 135 75 42 32 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2902 3774 4020 669 Size: 988 281 964 872 246 3079 Fragments arranged by size: 3079 988 964 872 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6430 HpaI GTT'AAC Cuts at: 4020 4020 Size: 6430 KpnI G_GTAC'C Cuts at: 3755 3755 Size: 6430 MfeI C'AATT_G Cuts at: 4029 4029 Size: 6430 MluI A'CGCG_T Cuts at: 6306 6306 Size: 6430 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2296 2968 5544 5728 1305 Size: 991 672 2576 184 2007 Fragments arranged by size: 2576 2007 991 672 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4608 Fragments arranged by size: 4608 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2294 2630 2885 3581 4388 4747 4906 Size: 648 1128 336 255 696 807 359 159 Cuts at: 4906 518 Size: 2042 Fragments arranged by size: 2042 1128 807 696 648 359 336 255 159 MspA1I CmG'CkG Cuts at: 1606 2390 2471 2528 2611 3846 4523 5671 5916 Size: 784 81 57 83 1235 677 1148 245 Cuts at: 5916 1606 Size: 2120 Fragments arranged by size: 2120 1235 1148 784 677 245 83 81 57 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5878 NdeI CA'TA_TG Cuts at: 387 387 Size: 6430 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6430 NotI GC'GGCC_GC Cuts at: 3877 3877 Size: 6430 NspI r_CATG'y Cuts at: 1132 1229 2428 3788 5333 1132 Size: 97 1199 1360 1545 2229 Fragments arranged by size: 2229 1545 1360 1199 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6430 PciI A'CATG_T Cuts at: 5329 5329 Size: 6430 PflMI CCAn_nnn'nTGG Cuts at: 1108 2148 1108 Size: 1040 5390 PfoI T'CCnGG_A Cuts at: 3004 3262 3288 3004 Size: 258 26 6146 Fragments arranged by size: 6146 258 26 PmeI GTTT'AAAC Cuts at: 3734 3734 Size: 6430 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4499 4531 6012 6044 4499 Size: 32 1481 32 4885 Fragments arranged by size: 4885 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2403 2403 Size: 6430 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6430 PsiI TTA'TAA Cuts at: 4000 4000 Size: 6430 PspOMI G'GGCC_C Cuts at: 2176 2176 Size: 6430 PstI C_TGCA'G Cuts at: 830 3214 3570 3782 830 Size: 2384 356 212 3478 Fragments arranged by size: 3478 2384 356 212 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6430 PvuII CAG'CTG Cuts at: 2528 2528 Size: 6430 SacII CC_GC'GG Cuts at: 2612 2612 Size: 6430 SalI G'TCGA_C Cuts at: 1936 2900 3772 1936 Size: 964 872 4594 Fragments arranged by size: 4594 964 872 SbfI CC_TGCA'GG Cuts at: 3782 3782 Size: 6430 ScaI AGT'ACT Cuts at: 4678 6390 4678 Size: 1712 4718 SfcI C'TryA_G Cuts at: 826 1045 3177 3210 3318 3566 3778 4913 5594 Size: 219 2132 33 108 248 212 1135 681 Cuts at: 5594 5785 826 Size: 191 1471 Fragments arranged by size: 2132 1471 1135 681 248 219 212 191 108 33 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6430 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6430 SmaI CCC'GGG Cuts at: 1872 2216 3757 1872 Size: 344 1541 4545 Fragments arranged by size: 4545 1541 344 SmlI C'TyrA_G Cuts at: 820 1017 1943 4534 5435 5697 820 Size: 197 926 2591 901 262 1553 Fragments arranged by size: 2591 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4221 493 Size: 3728 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6430 SphI G_CATG'C Cuts at: 1132 2428 3788 1132 Size: 1296 1360 3774 Fragments arranged by size: 3774 1360 1296 SspI AAT'ATT Cuts at: 5 52 4354 5 Size: 47 4302 2081 Fragments arranged by size: 4302 2081 47 StuI AGG'CCT Cuts at: 1517 2908 1517 Size: 1391 5039 StyI C'CwwG_G Cuts at: 513 1065 2181 3869 513 Size: 552 1116 1688 3074 Fragments arranged by size: 3074 1688 1116 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2033 3575 4457 4474 4627 4812 5257 Size: 1255 453 1542 882 17 153 185 445 Cuts at: 5257 325 Size: 1498 Fragments arranged by size: 1542 1498 1255 882 453 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4676 6388 96 Size: 274 80 33 51 1443 2699 1712 138 Fragments arranged by size: 2699 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3436 3468 6390 6422 3436 Size: 32 2922 32 3444 Fragments arranged by size: 3444 2922 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3452 1316 Size: 2136 4294 XbaI T'CTAG_A Cuts at: 3766 3766 Size: 6430 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2035 3388 2035 Size: 1353 5077 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6430 XmaI C'CCGG_G Cuts at: 1870 2214 3755 1870 Size: 344 1541 4545 Fragments arranged by size: 4545 1541 344 XmnI GAAnn'nnTTC Cuts at: 4559 4559 Size: 6430 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4238 276 Size: 53 83 186 936 2704 2468 Fragments arranged by size: 2704 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI SwaI