Restriction map for
pFN21AB9402
(Linear) (Six-base) MAPSORT of: KIBB9402.seq Check: 99 from: 1 to: 1725
KIBB9402.orf
With 182 enzymes: SgfI *
October 24, 2006 15:10 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 21 309 1725
Size: 21 288 1416
Fragments arranged by size:
1416 288 21
AccI GT'mk_AC
Cuts at: 0 896 1316 1725
Size: 896 420 409
Fragments arranged by size:
896 420 409
AclI AA'CG_TT
Cuts at: 0 644 1725
Size: 644 1081
AlwNI CAG_nnn'CTG
Cuts at: 0 1302 1560 1725
Size: 1302 258 165
Fragments arranged by size:
1302 258 165
ApaI G_GGCC'C
Cuts at: 0 175 1725
Size: 175 1550
ApaLI G'TGCA_C
Cuts at: 0 322 883 1725
Size: 322 561 842
Fragments arranged by size:
842 561 322
AvaI C'yCGr_G
Cuts at: 0 209 1408 1725
Size: 209 1199 317
Fragments arranged by size:
1199 317 209
BanI G'GyrC_C
Cuts at: 0 1582 1725
Size: 1582 143
BanII G_rGCy'C
Cuts at: 0 175 220 770 835 1725
Size: 175 45 550 65 890
Fragments arranged by size:
890 550 175 65 45
BbsI GAAGACnn'nnnn_
Cuts at: 0 694 1342 1725
Size: 694 648 383
Fragments arranged by size:
694 648 383
BbvCI CC'TCA_GC
Cuts at: 0 905 1725
Size: 905 820
BglI GCCn_nnn'nGGC
Cuts at: 0 830 1725
Size: 830 895
Bme1580I G_kGCm'C
Cuts at: 0 164 175 326 887 1725
Size: 164 11 151 561 838
Fragments arranged by size:
838 561 164 151 11
BmrI ACTGGGnnnn_n'
Cuts at: 0 396 507 771 973 1725
Size: 396 111 264 202 752
Fragments arranged by size:
752 396 264 202 111
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 503 1091 1306 1324 1513 1725
Size: 503 588 215 18 189 212
Fragments arranged by size:
588 503 215 212 189 18
Bpu10I CC'TnA_GC
Cuts at: 0 503 905 1725
Size: 503 402 820
Fragments arranged by size:
820 503 402
BsaAI yAC'GTr
Cuts at: 0 143 1725
Size: 143 1582
BsaBI GATnn'nnATC
Cuts at: 0 85 1725
Size: 85 1640
BsaHI Gr'CG_yC
Cuts at: 0 37 1725
Size: 37 1688
BsaWI w'CCGG_w
Cuts at: 0 624 1725
Size: 624 1101
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 977 1007 1431 1461 1725
Size: 977 30 424 30 264
Fragments arranged by size:
977 424 264 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 380 540 1112 1725
Size: 380 160 572 613
Fragments arranged by size:
613 572 380 160
BseYI C'CCAG_C
Cuts at: 0 260 344 479 486 662 699 944 1126
Size: 260 84 135 7 176 37 245 182
Cuts at: 1126 1192 1534 1646 1684 1713 1725
Size: 66 342 112 38 29 12
Fragments arranged by size:
342 260 245 182 176 135 112 84
66 38 37 29 12 7
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 917 1725
Size: 917 808
BsiHKAI G_wGCw'C
Cuts at: 0 296 326 887 1725
Size: 296 30 561 838
Fragments arranged by size:
838 561 296 30
BsmBI CGTCTCn'nnnn_
Cuts at: 0 1436 1725
Size: 1436 289
Bsp1286I G_dGCh'C
Cuts at: 0 164 175 220 296 326 770 835 887
Size: 164 11 45 76 30 444 65 52
Cuts at: 887 1725
Size: 838
Fragments arranged by size:
838 444 164 76 65 52 45 30
11
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 21 309 1725
Size: 21 288 1416
Fragments arranged by size:
1416 288 21
BsrBI CCG'CTC
Cuts at: 0 507 1725
Size: 507 1218
BsrFI r'CCGG_y
Cuts at: 0 100 314 1725
Size: 100 214 1411
Fragments arranged by size:
1411 214 100
BssSI C'ACGA_G
Cuts at: 0 634 1725
Size: 634 1091
BstEII G'GTnAC_C
Cuts at: 0 49 1708 1725
Size: 49 1659 17
Fragments arranged by size:
1659 49 17
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1167 1725
Size: 1167 558
BstYI r'GATC_y
Cuts at: 0 746 1629 1725
Size: 746 883 96
Fragments arranged by size:
883 746 96
BtgI C'CryG_G
Cuts at: 0 495 604 890 1586 1725
Size: 495 109 286 696 139
Fragments arranged by size:
696 495 286 139 109
BtsI GCAGTG_nn'
Cuts at: 0 1196 1558 1725
Size: 1196 362 167
Fragments arranged by size:
1196 362 167
DraIII CAC_nnn'GTG
Cuts at: 0 143 252 891 1725
Size: 143 109 639 834
Fragments arranged by size:
834 639 143 109
EaeI y'GGCC_r
Cuts at: 0 554 652 1725
Size: 554 98 1073
Fragments arranged by size:
1073 554 98
EarI CTCTTCn'nnn_
Cuts at: 0 1158 1172 1358 1725
Size: 1158 14 186 367
Fragments arranged by size:
1158 367 186 14
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 503 1091 1306 1324 1513 1725
Size: 503 588 215 18 189 212
Fragments arranged by size:
588 503 215 212 189 18
EcoNI CCTnn'n_nnAGG
Cuts at: 0 573 1288 1725
Size: 573 715 437
Fragments arranged by size:
715 573 437
EcoO109I rG'GnC_Cy
Cuts at: 0 171 172 398 1725
Size: 171 1 226 1327
Fragments arranged by size:
1327 226 171 1
HaeII r_GCGC'y
Cuts at: 0 8 308 1725
Size: 8 300 1417
Fragments arranged by size:
1417 300 8
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 65 97 172 204 523 555 977 1009
Size: 65 32 75 32 319 32 422 32
Cuts at: 1009 1433 1465 1725
Size: 424 32 260
Fragments arranged by size:
424 422 319 260 75 65 32 32
32 32 32
HincII GTy'rAC
Cuts at: 0 897 1725
Size: 897 828
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 291 963 1725
Size: 291 672 762
Fragments arranged by size:
762 672 291
MslI CAynn'nnrTG
Cuts at: 0 289 625 880 1576 1725
Size: 289 336 255 696 149
Fragments arranged by size:
696 336 289 255 149
MspA1I CmG'CkG
Cuts at: 0 385 466 523 606 1725
Size: 385 81 57 83 1119
Fragments arranged by size:
1119 385 83 81 57
NspI r_CATG'y
Cuts at: 0 423 1725
Size: 423 1302
PflMI CCAn_nnn'nTGG
Cuts at: 0 143 1725
Size: 143 1582
PfoI T'CCnGG_A
Cuts at: 0 999 1257 1283 1725
Size: 999 258 26 442
Fragments arranged by size:
999 442 258 26
PpuMI rG'GwC_Cy
Cuts at: 0 398 1725
Size: 398 1327
PspOMI G'GGCC_C
Cuts at: 0 171 1725
Size: 171 1554
PstI C_TGCA'G
Cuts at: 0 1209 1565 1725
Size: 1209 356 160
Fragments arranged by size:
1209 356 160
PvuII CAG'CTG
Cuts at: 0 523 1725
Size: 523 1202
SacII CC_GC'GG
Cuts at: 0 607 1725
Size: 607 1118
SalI G'TCGA_C
Cuts at: 0 895 1725
Size: 895 830
SfcI C'TryA_G
Cuts at: 0 1172 1205 1313 1561 1725
Size: 1172 33 108 248 164
Fragments arranged by size:
1172 248 164 108 33
SmaI CCC'GGG
Cuts at: 0 211 1725
Size: 211 1514
SphI G_CATG'C
Cuts at: 0 423 1725
Size: 423 1302
StuI AGG'CCT
Cuts at: 0 903 1725
Size: 903 822
StyI C'CwwG_G
Cuts at: 0 176 1725
Size: 176 1549
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 28 1570 1725
Size: 28 1542 155
Fragments arranged by size:
1542 155 28
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1431 1463 1725
Size: 1431 32 262
Fragments arranged by size:
1431 262 32
Tth111I GACn'n_nGTC
Cuts at: 0 1447 1725
Size: 1447 278
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 30 1383 1725
Size: 30 1353 342
Fragments arranged by size:
1353 342 30
XmaI C'CCGG_G
Cuts at: 0 209 1725
Size: 209 1516
Enzymes that do cut:
AarI AccI AclI AlwNI ApaI ApaLI AvaI BanI
BanII BbsI BbvCI BglI Bme1580I BmrI BpmI Bpu10I
BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI
BsiHKAI BsmBI Bsp1286I BspMI BsrBI BsrFI BssSI BstEII
BstXI BstYI BtgI BtsI DraIII EaeI EarI Eco57MI
EcoNI EcoO109I HaeII Hin4I HincII MmeI MslI MspA1I
NspI PflMI PfoI PpuMI PspOMI PstI PvuII SacII
SalI SfcI SmaI SphI StuI StyI TaqII TstI
Tth111I XcmI XmaI
Enzymes that do not cut:
AatII Acc65I AcuI AfeI AflII AflIII AgeI AhdI
AleI AlfI AloI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BcgI BciVI BclI BfrBI BglII
BlpI BmgBI BmtI BplI BpuEI BsaI BsiEI BsiWI
BsmI BspEI BspHI BsrDI BsrGI BssHII BstAPI BstBI
BstZ17I Bsu36I BtgZI ClaI CspCI DraI DrdI EagI
EciI EcoICRI EcoRI EcoRV FalI FseI FspI FspAI
HindIII HpaI KasI KpnI MfeI MluI MscI NaeI
NarI NcoI NdeI NgoMIV NheI NotI NruI NsiI
PacI PasI PciI PmeI PmlI PpiI PshAI PsiI
PspXI PsrI PvuI RsrII SacI SanDI SapI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmlI SnaBI
SpeI SrfI SspI SwaI TatI XbaI XhoI XmnI
ZraI