(Circular) (Six-base) MAPSORT of: pFN21AB8009.seq Check: 3942 from: 1 to: 5854 pFN21AB8009 5854 bp With 182 enzymes: SgfI * January 16, 2009 15:26 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2084 3664 278 Size: 53 83 186 936 548 1580 2468 Fragments arranged by size: 2468 1580 936 548 186 83 53 AccI GT'mk_AC Cuts at: 1937 3197 1937 Size: 1260 4594 Acc65I G'GTAC_C Cuts at: 2884 3175 2884 Size: 291 5563 AclI AA'CG_TT Cuts at: 1563 3981 4354 1563 Size: 2418 373 3063 Fragments arranged by size: 3063 2418 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2520 2613 2912 2990 3917 5301 5569 1632 Size: 888 93 299 78 927 1384 268 1917 Fragments arranged by size: 1917 1384 927 888 299 268 93 78 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5657 AflIII A'CryG_T Cuts at: 1203 4753 5730 1203 Size: 3550 977 1327 Fragments arranged by size: 3550 1327 977 AgeI A'CCGG_T Cuts at: 4685 4685 Size: 5854 AhdI GACnn_n'nnGTC Cuts at: 4583 4583 Size: 5854 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5854 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2817 3144 1184 Size: 1633 327 3894 Fragments arranged by size: 3894 1633 327 AlwNI CAG_nnn'CTG Cuts at: 3597 5169 3597 Size: 1572 4282 ApaLI G'TGCA_C Cuts at: 2457 3911 5067 2457 Size: 1454 1156 3244 Fragments arranged by size: 3244 1454 1156 ApoI r'AATT_y Cuts at: 1493 2572 2744 2913 3148 3163 3389 3541 5650 Size: 1079 172 169 235 15 226 152 2109 Cuts at: 5650 1493 Size: 1697 Fragments arranged by size: 2109 1697 1079 235 226 172 169 152 15 AseI AT'TA_AT Cuts at: 160 1032 4408 160 Size: 872 3376 1606 Fragments arranged by size: 3376 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5854 AvaI C'yCGr_G Cuts at: 1870 1943 3179 1870 Size: 73 1236 4545 Fragments arranged by size: 4545 1236 73 BamHI G'GATC_C Cuts at: 3184 3551 3184 Size: 367 5487 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2884 3175 4630 618 Size: 325 217 627 1097 291 1455 1842 Fragments arranged by size: 1842 1455 1097 627 325 291 217 BanII G_rGCy'C Cuts at: 1391 2947 3173 1391 Size: 1556 226 4072 Fragments arranged by size: 4072 1556 226 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2150 2472 928 Size: 971 251 322 4310 Fragments arranged by size: 4310 971 322 251 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4045 4079 1903 Size: 34 50 34 2024 34 3678 Fragments arranged by size: 3678 2024 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2995 3746 4956 2995 Size: 751 1210 3893 Fragments arranged by size: 3893 1210 751 BclI T'GATC_A Cuts at: 1548 2824 1548 Size: 1276 4578 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4465 136 Size: 107 122 71 631 605 2793 1525 Fragments arranged by size: 2793 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5849 5849 Size: 5854 BlpI GC'TnA_GC Cuts at: 3271 3271 Size: 5854 Bme1580I G_kGCm'C Cuts at: 1790 2461 3915 5071 1790 Size: 671 1454 1156 2573 Fragments arranged by size: 2573 1454 1156 671 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5854 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3585 4543 442 Size: 416 53 83 399 24 2168 958 1753 Fragments arranged by size: 2168 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5854 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2709 2709 Size: 5854 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2214 2704 4514 1239 Size: 975 490 1810 2579 Fragments arranged by size: 2579 1810 975 490 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2717 3979 4844 5142 2717 Size: 1262 865 298 3429 Fragments arranged by size: 3429 1262 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4517 882 Size: 617 3018 2219 Fragments arranged by size: 3018 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2068 3645 493 Size: 711 864 1577 2702 Fragments arranged by size: 2702 1577 864 711 BsaBI GATnn'nnATC Cuts at: 5848 5848 Size: 5854 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2081 3661 4043 4678 Size: 53 83 186 936 548 1580 382 635 Cuts at: 4678 275 Size: 1451 Fragments arranged by size: 1580 1451 936 635 548 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4287 4685 4959 5106 5517 4287 Size: 398 274 147 411 4624 Fragments arranged by size: 4624 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2187 2217 1178 Size: 30 165 30 784 30 4815 Fragments arranged by size: 4815 784 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 5854 BseYI C'CCAG_C Cuts at: 5057 5553 5057 Size: 496 5358 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2075 2271 1163 Size: 573 339 196 4746 Fragments arranged by size: 4746 573 339 196 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2049 3304 4065 5093 1540 Size: 275 186 48 1255 761 1028 2301 Fragments arranged by size: 2301 1255 1028 761 275 186 48 BsiHKAI G_wGCw'C Cuts at: 2461 2806 3915 4000 5071 2461 Size: 345 1109 85 1071 3244 Fragments arranged by size: 3244 1109 1071 345 85 BsmI GAATG_Cn' Cuts at: 2514 3021 3365 3458 2514 Size: 507 344 93 4910 Fragments arranged by size: 4910 507 344 93 BsmBI CGTCTCn'nnnn_ Cuts at: 2072 2072 Size: 5854 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2461 2806 2947 3173 3915 4000 5071 Size: 399 671 345 141 226 742 85 1071 Cuts at: 5071 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 671 399 345 226 141 85 BspEI T'CCGG_A Cuts at: 5517 5517 Size: 5854 BspHI T'CATG_A Cuts at: 2251 3033 3743 5473 2251 Size: 782 710 1730 2632 Fragments arranged by size: 2632 1730 782 710 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2896 3209 844 Size: 2052 313 3489 Fragments arranged by size: 3489 2052 313 BsrBI CCG'CTC Cuts at: 1394 1823 3741 1394 Size: 429 1918 3507 Fragments arranged by size: 3507 1918 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4349 4523 65 Size: 1183 3101 174 1396 Fragments arranged by size: 3101 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2044 4498 4685 1700 Size: 110 234 2454 187 2869 Fragments arranged by size: 2869 2454 234 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5854 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5854 BssSI C'ACGA_G Cuts at: 2940 3914 4926 2940 Size: 974 1012 3868 Fragments arranged by size: 3868 1012 974 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5854 BstBI TT'CG_AA Cuts at: 4658 4658 Size: 5854 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5854 BstXI CCAn_nnnn'nTGG Cuts at: 3140 3140 Size: 5854 BstYI r'GATC_y Cuts at: 1972 3184 3551 3936 3953 5405 5491 5503 5849 Size: 1212 367 385 17 1452 86 12 346 Cuts at: 5849 1972 Size: 1977 Fragments arranged by size: 1977 1452 1212 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2584 513 Size: 552 1519 3783 Fragments arranged by size: 3783 1519 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5115 Fragments arranged by size: 5115 683 56 BtsI GCAGTG_nn' Cuts at: 3128 3370 4164 4184 3128 Size: 242 794 20 4798 Fragments arranged by size: 4798 794 242 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5854 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5819 DraI TTT'AAA Cuts at: 3158 3158 Size: 5854 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4861 809 Size: 507 3545 1802 Fragments arranged by size: 3545 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2046 2441 3301 Size: 54 1425 50 275 18 216 395 860 Cuts at: 3301 4190 5819 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 860 395 275 216 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2046 3301 1537 Size: 275 234 1255 4090 Fragments arranged by size: 4090 1255 275 234 EarI CTCTTCn'nnn_ Cuts at: 1353 2260 2504 2987 3035 3784 1353 Size: 907 244 483 48 749 3423 Fragments arranged by size: 3423 907 749 483 244 48 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4441 4815 4961 387 Size: 1064 138 2852 374 146 1280 Fragments arranged by size: 2852 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2214 2520 2613 2704 2912 2990 3917 Size: 393 582 306 93 91 208 78 927 Cuts at: 3917 4514 5301 5569 1239 Size: 597 787 268 1524 Fragments arranged by size: 1524 927 787 597 582 393 306 268 208 93 91 78 EcoRI G'AATT_C Cuts at: 3163 3541 3163 Size: 378 5476 EcoRV GAT'ATC Cuts at: 2152 2152 Size: 5854 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 3046 3104 3046 Size: 58 5796 FspI TGC'GCA Cuts at: 4360 4360 Size: 5854 HaeII r_GCGC'y Cuts at: 1678 5001 1678 Size: 3323 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2071 2103 4495 4527 4569 Size: 32 135 32 499 32 2392 32 42 Cuts at: 4569 4601 1373 Size: 32 2626 Fragments arranged by size: 2626 2392 499 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3198 3444 669 Size: 988 281 1260 246 3079 Fragments arranged by size: 3079 1260 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5854 HpaI GTT'AAC Cuts at: 3444 3444 Size: 5854 KpnI G_GTAC'C Cuts at: 2888 3179 2888 Size: 291 5563 MfeI C'AATT_G Cuts at: 3453 3453 Size: 5854 MluI A'CGCG_T Cuts at: 5730 5730 Size: 5854 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2153 3078 4968 5152 1305 Size: 848 925 1890 184 2007 Fragments arranged by size: 2007 1890 925 848 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4032 Fragments arranged by size: 4032 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2799 3812 4171 4330 518 Size: 648 1633 1013 359 159 2042 Fragments arranged by size: 2042 1633 1013 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2989 3270 3947 5095 5340 1606 Size: 1383 281 677 1148 245 2120 Fragments arranged by size: 2120 1383 1148 677 281 245 NaeI GCC'GGC Cuts at: 2046 2046 Size: 5854 NcoI C'CATG_G Cuts at: 513 1065 2584 513 Size: 552 1519 3783 Fragments arranged by size: 3783 1519 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5854 NgoMIV G'CCGG_C Cuts at: 2044 2044 Size: 5854 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5854 NotI GC'GGCC_GC Cuts at: 3301 3301 Size: 5854 NspI r_CATG'y Cuts at: 1132 1229 2534 3212 4757 1132 Size: 97 1305 678 1545 2229 Fragments arranged by size: 2229 1545 1305 678 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5854 PciI A'CATG_T Cuts at: 4753 4753 Size: 5854 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5854 PfoI T'CCnGG_A Cuts at: 2716 2716 Size: 5854 PmeI GTTT'AAAC Cuts at: 3158 3158 Size: 5854 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3923 3955 5436 5468 3923 Size: 32 1481 32 4309 Fragments arranged by size: 4309 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5854 PsiI TTA'TAA Cuts at: 3424 3424 Size: 5854 PstI C_TGCA'G Cuts at: 830 2911 3206 830 Size: 2081 295 3478 Fragments arranged by size: 3478 2081 295 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5854 PvuII CAG'CTG Cuts at: 2989 2989 Size: 5854 SalI G'TCGA_C Cuts at: 1936 3196 1936 Size: 1260 4594 SapI GCTCTTCn'nnn_ Cuts at: 3035 3035 Size: 5854 SbfI CC_TGCA'GG Cuts at: 3206 3206 Size: 5854 ScaI AGT'ACT Cuts at: 4102 5814 4102 Size: 1712 4142 SfcI C'TryA_G Cuts at: 826 1045 2470 2907 3202 4337 5018 5209 826 Size: 219 1425 437 295 1135 681 191 1471 Fragments arranged by size: 1471 1425 1135 681 437 295 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5854 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5854 SmaI CCC'GGG Cuts at: 1872 3181 1872 Size: 1309 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2696 3958 4859 5121 820 Size: 197 926 753 1262 901 262 1553 Fragments arranged by size: 1553 1262 926 901 753 262 197 SnaBI TAC'GTA Cuts at: 493 3645 493 Size: 3152 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5854 SphI G_CATG'C Cuts at: 1132 2534 3212 1132 Size: 1402 678 3774 Fragments arranged by size: 3774 1402 678 SspI AAT'ATT Cuts at: 5 52 2651 3778 5 Size: 47 2599 1127 2081 Fragments arranged by size: 2599 2081 1127 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5854 StyI C'CwwG_G Cuts at: 513 1065 2584 3293 513 Size: 552 1519 709 3074 Fragments arranged by size: 3074 1519 709 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3881 3898 4051 4236 4681 325 Size: 1255 2301 17 153 185 445 1498 Fragments arranged by size: 2301 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4100 5812 96 Size: 274 80 33 51 1443 2123 1712 138 Fragments arranged by size: 2123 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5814 5846 5814 Size: 32 5822 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5854 XbaI T'CTAG_A Cuts at: 3190 3190 Size: 5854 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5854 XmaI C'CCGG_G Cuts at: 1870 3179 1870 Size: 1309 4545 XmnI GAAnn'nnTTC Cuts at: 2709 3983 2709 Size: 1274 4580 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2082 3662 276 Size: 53 83 186 936 548 1580 2468 Fragments arranged by size: 2468 1580 936 548 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoO109I FseI FspAI KasI NarI NruI NsiI PacI PmlI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI