(Circular) (Six-base) MAPSORT of: pFN21AB8001.seq Check: 9003 from: 1 to: 5500 pFN21AB8001 5500 bp With 182 enzymes: SgfI * October 20, 2009 11:00 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3310 278 Size: 53 83 186 936 1774 2468 Fragments arranged by size: 2468 1774 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2451 2843 1937 Size: 514 392 4594 Fragments arranged by size: 4594 514 392 Acc65I G'GTAC_C Cuts at: 2821 2821 Size: 5500 AclI AA'CG_TT Cuts at: 1563 3627 4000 1563 Size: 2064 373 3063 Fragments arranged by size: 3063 2064 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2322 3563 4947 5215 1632 Size: 690 1241 1384 268 1917 Fragments arranged by size: 1917 1384 1241 690 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5303 AflIII A'CryG_T Cuts at: 1203 4399 5376 1203 Size: 3196 977 1327 Fragments arranged by size: 3196 1327 977 AgeI A'CCGG_T Cuts at: 4331 4331 Size: 5500 AhdI GACnn_n'nnGTC Cuts at: 4229 4229 Size: 5500 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5500 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5500 AlwNI CAG_nnn'CTG Cuts at: 2300 2711 2753 3243 4815 2300 Size: 411 42 490 1572 2985 Fragments arranged by size: 2985 1572 490 411 42 ApaI G_GGCC'C Cuts at: 2718 2718 Size: 5500 ApaLI G'TGCA_C Cuts at: 3557 4713 3557 Size: 1156 4344 ApoI r'AATT_y Cuts at: 1493 2809 3035 3187 5296 1493 Size: 1316 226 152 2109 1697 Fragments arranged by size: 2109 1697 1316 226 152 AseI AT'TA_AT Cuts at: 160 1032 4054 160 Size: 872 3022 1606 Fragments arranged by size: 3022 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5500 AvaI C'yCGr_G Cuts at: 1870 1943 2643 2825 1870 Size: 73 700 182 4545 Fragments arranged by size: 4545 700 182 73 BamHI G'GATC_C Cuts at: 2830 3197 2830 Size: 367 5133 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2532 2821 4276 618 Size: 325 217 627 745 289 1455 1842 Fragments arranged by size: 1842 1455 745 627 325 289 217 BanII G_rGCy'C Cuts at: 1391 2190 2718 2819 1391 Size: 799 528 101 4072 Fragments arranged by size: 4072 799 528 101 BbeI G_GCGC'C Cuts at: 2536 2536 Size: 5500 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4529 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3691 3725 1903 Size: 34 50 34 1670 34 3678 Fragments arranged by size: 3678 1670 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2233 2748 3392 4602 2233 Size: 515 644 1210 3131 Fragments arranged by size: 3131 1210 644 515 BclI T'GATC_A Cuts at: 1548 1548 Size: 5500 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4111 136 Size: 107 122 71 631 605 2439 1525 Fragments arranged by size: 2439 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5495 5495 Size: 5500 BlpI GC'TnA_GC Cuts at: 2917 2917 Size: 5500 Bme1580I G_kGCm'C Cuts at: 1790 2651 2718 3561 4717 1790 Size: 861 67 843 1156 2573 Fragments arranged by size: 2573 1156 861 843 67 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5500 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3231 4189 442 Size: 416 53 83 399 24 1814 958 1753 Fragments arranged by size: 1814 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5500 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2069 2069 Size: 5500 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2084 4160 1239 Size: 845 2076 2579 Fragments arranged by size: 2579 2076 845 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3625 4490 4788 3625 Size: 865 298 4337 Fragments arranged by size: 4337 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4163 882 Size: 617 2664 2219 Fragments arranged by size: 2664 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2652 3291 493 Size: 711 1448 639 2702 Fragments arranged by size: 2702 1448 711 639 BsaBI GATnn'nnATC Cuts at: 5494 5494 Size: 5500 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2533 2637 3307 3689 Size: 53 83 186 936 1000 104 670 382 Cuts at: 3689 4324 275 Size: 635 1451 Fragments arranged by size: 1451 1000 936 670 635 382 186 104 83 53 BsaWI w'CCGG_w Cuts at: 2316 3933 4331 4605 4752 5163 2316 Size: 1617 398 274 147 411 2653 Fragments arranged by size: 2653 1617 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2234 2264 1178 Size: 30 165 30 831 30 4414 Fragments arranged by size: 4414 831 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2564 1186 Size: 1378 4122 BseYI C'CCAG_C Cuts at: 2711 4703 5199 2711 Size: 1992 496 3012 Fragments arranged by size: 3012 1992 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4927 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2735 2950 3711 4739 1540 Size: 275 186 734 215 761 1028 2301 Fragments arranged by size: 2301 1028 761 734 275 215 186 BsiHKAI G_wGCw'C Cuts at: 2760 3561 3646 4717 2760 Size: 801 85 1071 3543 Fragments arranged by size: 3543 1071 801 85 BsmI GAATG_Cn' Cuts at: 2209 3011 3104 2209 Size: 802 93 4605 Fragments arranged by size: 4605 802 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2190 2651 2718 2760 2819 3561 3646 Size: 399 400 461 67 42 59 742 85 Cuts at: 3646 4717 1391 Size: 1071 2174 Fragments arranged by size: 2174 1071 742 461 400 399 85 67 59 42 BspEI T'CCGG_A Cuts at: 5163 5163 Size: 5500 BspHI T'CATG_A Cuts at: 3389 5119 3389 Size: 1730 3770 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2855 844 Size: 2011 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3387 1394 Size: 429 1564 3507 Fragments arranged by size: 3507 1564 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2088 3995 4169 65 Size: 1183 840 1907 174 1396 Fragments arranged by size: 1907 1396 1183 840 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4144 4331 1700 Size: 110 2334 187 2869 Fragments arranged by size: 2869 2334 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5500 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5500 BssSI C'ACGA_G Cuts at: 3560 4572 3560 Size: 1012 4488 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5500 BstBI TT'CG_AA Cuts at: 4304 4304 Size: 5500 BstEII G'GTnAC_C Cuts at: 1184 2257 1184 Size: 1073 4427 BstXI CCAn_nnnn'nTGG Cuts at: 2137 2157 2254 2137 Size: 20 97 5383 Fragments arranged by size: 5383 97 20 BstYI r'GATC_y Cuts at: 1972 2830 3197 3582 3599 5051 5137 5149 5495 Size: 858 367 385 17 1452 86 12 346 Cuts at: 5495 1972 Size: 1977 Fragments arranged by size: 1977 1452 858 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2253 2491 2613 513 Size: 552 1188 238 122 3400 Fragments arranged by size: 3400 1188 552 238 122 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2525 487 Size: 683 56 1299 3462 Fragments arranged by size: 3462 1299 683 56 BtsI GCAGTG_nn' Cuts at: 3016 3810 3830 3016 Size: 794 20 4686 Fragments arranged by size: 4686 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5500 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5465 DraI TTT'AAA Cuts at: 2804 2804 Size: 5500 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4507 809 Size: 507 3191 1802 Fragments arranged by size: 3191 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2037 2268 2732 Size: 54 1425 50 275 18 207 231 464 Cuts at: 2732 2947 3836 5465 8 Size: 215 889 1629 43 Fragments arranged by size: 1629 1425 889 464 275 231 215 207 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2732 2947 1537 Size: 275 920 215 4090 Fragments arranged by size: 4090 920 275 215 EarI CTCTTCn'nnn_ Cuts at: 1353 2076 2511 2595 2726 3430 1353 Size: 723 435 84 131 704 3423 Fragments arranged by size: 3423 723 704 435 131 84 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4087 4461 4607 387 Size: 1064 138 2498 374 146 1280 Fragments arranged by size: 2498 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2084 2322 3563 4160 4947 5215 1239 Size: 393 452 238 1241 597 787 268 1524 Fragments arranged by size: 1524 1241 787 597 452 393 268 238 EcoO109I rG'GnC_Cy Cuts at: 2143 2715 2143 Size: 572 4928 EcoRI G'AATT_C Cuts at: 2809 3187 2809 Size: 378 5122 FspI TGC'GCA Cuts at: 4006 4006 Size: 5500 HaeII r_GCGC'y Cuts at: 1678 2536 2697 4647 1678 Size: 858 161 1950 2531 Fragments arranged by size: 2531 1950 858 161 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2207 2234 2239 2266 2609 Size: 32 135 32 635 27 5 27 343 Cuts at: 2609 2641 4141 4173 4215 4247 1373 Size: 32 1500 32 42 32 2626 Fragments arranged by size: 2626 1500 635 343 135 42 32 32 32 32 32 27 27 5 HincII GTy'rAC Cuts at: 669 1657 1938 2658 2844 3090 669 Size: 988 281 720 186 246 3079 Fragments arranged by size: 3079 988 720 281 246 186 HindIII A'AGCT_T Cuts at: 748 748 Size: 5500 HpaI GTT'AAC Cuts at: 3090 3090 Size: 5500 KasI G'GCGC_C Cuts at: 2532 2532 Size: 5500 KpnI G_GTAC'C Cuts at: 2825 2825 Size: 5500 MfeI C'AATT_G Cuts at: 3099 3099 Size: 5500 MluI A'CGCG_T Cuts at: 5376 5376 Size: 5500 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2118 2292 2313 2334 4614 4798 1305 Size: 813 174 21 21 2280 184 2007 Fragments arranged by size: 2280 2007 813 184 174 21 21 MscI TGG'CCA Cuts at: 10 64 1489 1832 2039 10 Size: 54 1425 343 207 3471 Fragments arranged by size: 3471 1425 343 207 54 MslI CAynn'nnrTG Cuts at: 518 1166 3458 3817 3976 518 Size: 648 2292 359 159 2042 Fragments arranged by size: 2292 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2011 2493 2711 2731 2916 3593 4741 4986 Size: 405 482 218 20 185 677 1148 245 Cuts at: 4986 1606 Size: 2120 Fragments arranged by size: 2120 1148 677 482 405 245 218 185 20 NarI GG'CG_CC Cuts at: 2533 2533 Size: 5500 NcoI C'CATG_G Cuts at: 513 1065 2613 513 Size: 552 1548 3400 Fragments arranged by size: 3400 1548 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5500 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5500 NotI GC'GGCC_GC Cuts at: 2947 2947 Size: 5500 NspI r_CATG'y Cuts at: 1132 1229 2858 4403 1132 Size: 97 1629 1545 2229 Fragments arranged by size: 2229 1629 1545 97 PasI CC'CwG_GG Cuts at: 1348 2747 1348 Size: 1399 4101 PciI A'CATG_T Cuts at: 4399 4399 Size: 5500 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5500 PmeI GTTT'AAAC Cuts at: 2804 2804 Size: 5500 PmlI CAC'GTG Cuts at: 2652 2652 Size: 5500 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3569 3601 5082 5114 3569 Size: 32 1481 32 3955 Fragments arranged by size: 3955 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5500 PsiI TTA'TAA Cuts at: 3070 3070 Size: 5500 PspOMI G'GGCC_C Cuts at: 2714 2714 Size: 5500 PsrI GAACnnnnnnTACnnnnnnn_nnnnn' Cuts at: 2153 2185 2153 Size: 32 5468 PstI C_TGCA'G Cuts at: 830 2028 2852 830 Size: 1198 824 3478 Fragments arranged by size: 3478 1198 824 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5500 PvuII CAG'CTG Cuts at: 2711 2711 Size: 5500 RsrII CG'GwC_CG Cuts at: 2356 2356 Size: 5500 SacII CC_GC'GG Cuts at: 2494 2494 Size: 5500 SalI G'TCGA_C Cuts at: 1936 2842 1936 Size: 906 4594 SbfI CC_TGCA'GG Cuts at: 2852 2852 Size: 5500 ScaI AGT'ACT Cuts at: 3748 5460 3748 Size: 1712 3788 SexAI A'CCwGG_T Cuts at: 2132 2132 Size: 5500 SfcI C'TryA_G Cuts at: 826 1045 2024 2848 3983 4664 4855 826 Size: 219 979 824 1135 681 191 1471 Fragments arranged by size: 1471 1135 979 824 681 219 191 SfoI GGC'GCC Cuts at: 2534 2534 Size: 5500 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5500 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5500 SmaI CCC'GGG Cuts at: 1872 2645 2827 1872 Size: 773 182 4545 Fragments arranged by size: 4545 773 182 SmlI C'TyrA_G Cuts at: 820 1017 1943 3604 4505 4767 820 Size: 197 926 1661 901 262 1553 Fragments arranged by size: 1661 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3291 493 Size: 2798 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5500 SphI G_CATG'C Cuts at: 1132 2858 1132 Size: 1726 3774 SspI AAT'ATT Cuts at: 5 52 3424 5 Size: 47 3372 2081 Fragments arranged by size: 3372 2081 47 StuI AGG'CCT Cuts at: 1517 2777 1517 Size: 1260 4240 StyI C'CwwG_G Cuts at: 513 1065 2613 2939 513 Size: 552 1548 326 3074 Fragments arranged by size: 3074 1548 552 326 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3527 3544 3697 3882 4327 325 Size: 1255 1947 17 153 185 445 1498 Fragments arranged by size: 1947 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3746 5458 96 Size: 274 80 33 51 1443 1769 1712 138 Fragments arranged by size: 1769 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5460 5492 5460 Size: 32 5468 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5500 XbaI T'CTAG_A Cuts at: 2836 2836 Size: 5500 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5500 XmaI C'CCGG_G Cuts at: 1870 2643 2825 1870 Size: 773 182 4545 Fragments arranged by size: 4545 773 182 XmnI GAAnn'nnTTC Cuts at: 3629 3629 Size: 5500 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3308 276 Size: 53 83 186 936 1774 2468 Fragments arranged by size: 2468 1774 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI PsrI PstI PvuI PvuII RsrII SacII SalI SbfI ScaI SexAI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BsmBI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PpuMI PspXI SacI SanDI SapI SfiI SrfI SwaI XcmI