Restriction map for
pFN21AB7014
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# NO CUT ENZYMES ( 49 enzymes) #
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Acc65I AccIII AclI AflII AgeI
AscI AseI AsnI Asp718I AvrII
BciVI BfrI BlnI BseAI BspEI
Bst1107I BstZ17I CpoI DraI EcoRV
EcoT22I HindIII HpaI KpnI MfeI
MluI MroI MunI NheI NotI
NruI NsiI PacI PaeR7I PinAI
PmeI PshBI Psp1406I PvuI RsrII
SacI SalI SfiI SnaBI SpeI
SspI SwaI XbaI XhoI
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# ONE CUT ENZYMES ( 30 enzymes) #
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AccI 820 GTCTAC GT^[AC][GT]AC
AhdI 754 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Alw44I 2109 GTGCAC G^TGCAC
ApaLI 2109 GTGCAC G^TGCAC
Asp700I 4146 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 754 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
BamHI 1895 GGATCC G^GATCC
BbrPI 2104 CACGTG CAC^GTG
BsaBI 2988 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsiWI 935 CGTACG C^GTACG
BspHI 4232 TCATGA T^CATGA
BssHII 4744 GCGCGC G^CGCGC
BstBI 2731 TTCGAA TT^CGAA
Eam1105I 754 GACACGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EcoRI 2358 GAATTC G^AATTC
MamI 2988 GATTGGCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MseI 1160 TTAA T^TAA
NcoI 3356 CCATGG C^CATGG
NdeI 4031 CATATG CA^TATG
NspV 2731 TTCGAA TT^CGAA
PmaCI 2104 CACGTG CAC^GTG
PmlI 2104 CACGTG CAC^GTG
PshAI 2737 GACGTGCGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
RcaI 4232 TCATGA T^CATGA
SfuI 2731 TTCGAA TT^CGAA
SgrAI 870 CGCCGGCG C[GA]^CCGG[CT]G
SphI 4928 GCATGC GCATG^C
StuI 1808 AGGCCT AGG^CCT
Tru9I 1160 TTAA T^TAA
XmnI 4146 GAAGCACTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 40 enzymes) #
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AatII 4768 GACGTC GACGT^C
4866 GACGTC
AcsI 925 AAATTC [GA]^AATT[CT]
2358 GAATTC
ApoI 925 AAATTC [GA]^AATT[CT]
2358 GAATTC
AviII 3392 TGCGCA TGC^GCA
4934 TGCGCA
BbsI 2734 GAAGAC GAAGAC(2/6)
2023 GTCTTC
BclI 4979 TGATCA T^GATCA
5312 TGATCA
BfaI 214 CTAG C^TAG
841 CTAG
BlpI 5177 GCTCAGC GC^T[ACGT]AGC
5560 GCTGAGC
Bpu1102I 5177 GCTCAGC GC^T[ACGT]AGC
5560 GCTGAGC
BpuAI 2734 GAAGAC GAAGAC(2/6)
2023 GTCTTC
BsaAI 2104 CACGTG [CT]AC^GT[GA]
2200 TACGTG
BsaWI 3086 TCCGGT [AT]^CCGG[AT]
4025 ACCGGA
BseRI 4890 CTCCTC GAGGAG(10/8)
682 GAGGAG
BsmBI 2668 GAGACG CGTCTC(1/5)
3661 GAGACG
BsmI 3937 GAATGC GAATGC(1/-1)
4593 GAATGC
Bsp1407I 794 TGTACA T^GTACA
3341 TGTACA
BspDI 625 ATCGAT AT^CGAT
1258 ATCGAT
BspLU11I 695 ACATGT A^CATGT
5204 ACATGT
BsrDI 573 CATTGC GCAATG(2/0)
1766 GCAATG
BsrGI 794 TGTACA T^GTACA
3341 TGTACA
BstEII 4635 GGTCACC G^GT[ACGT]ACC
5056 GGTGACC
BstPI 4635 GGTCACC G^GT[ACGT]ACC
5056 GGTGACC
BstXI 2225 CCATCACCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
3986 CCAATGACCTGG
CelII 5177 GCTCAGC GC^T[ACGT]AGC
5560 GCTGAGC
ClaI 625 ATCGAT AT^CGAT
1258 ATCGAT
DrdI 4762 GACGAGGACGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
4830 GACAGACGGGTC
EagI 1014 CGGCCG C^GGCCG
3418 CGGCCG
EclXI 1014 CGGCCG C^GGCCG
3418 CGGCCG
Eco52I 1014 CGGCCG C^GGCCG
3418 CGGCCG
EcoO65I 4635 GGTCACC G^GT[ACGT]ACC
5056 GGTGACC
FbaI 4979 TGATCA T^GATCA
5312 TGATCA
FseI 1459 GGCCGGCC GGCCGG^CC
3046 GGCCGGCC
FspI 3392 TGCGCA TGC^GCA
4934 TGCGCA
MaeI 214 CTAG C^TAG
841 CTAG
SapI 5274 GAAGAGC GCTCTTC(1/4)
606 GCTCTTC
ScaI 382 AGTACT AGT^ACT
641 AGTACT
SexAI 4823 ACCAGGT A^CC[AT]GGT
5018 ACCTGGT
SspBI 794 TGTACA T^GTACA
3341 TGTACA
Tsp509I 926 AATT ^AATT
2359 AATT
XcmI 602 CCAAGCTCTTCGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
3524 CCACTGGCCTGCTGG
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# 3 OR MORE CUT ENZYMES ( 139 enzymes) #
#########################################
AccII AciI AcyI AfaI AflIII
AluI AlwI AlwNI AocI Aor51HI
ApaI AspHI AspI AvaI AvaII
BalI BanI BanII BbeI BbvCI
BbvI BcgI BcnI BglI BglII
BmyI BpmI BsaHI BsaI BsaJI
BsgI BsiEI BsiHKAI BsiYI BslI
BsmAI BsmFI BsoBI Bsp1286I BspMI
BsrBI BsrFI BsrI BssKI BssSI
BstAPI BstF5I BstNI BstUI BstYI
Bsu36I Cac8I CfoI Cfr10I Cfr13I
DdeI DpnII DraII DraIII DsaI
EaeI EarI Eco47III Eco81I EcoNI
EcoO109I EcoRII EcoT14I FauI Fnu4HI
FokI HaeII HaeIII HapII HgaI
HhaI Hin1I HinP1I HincII HindII
HinfI HpaII HphI ItaI KasI
Ksp632I KspI MaeII MaeIII MboI
MboII MflI MluNI MnlI MscI
MslI MspA1I MspI MvaI MvnI
MwoI NaeI NarI NciI NdeII
NgoMIV NlaIII NlaIV NspI PflFI
PflMI PleI PpuMI PstI PvuII
RsaI SacII Sau3AI Sau96I SbfI
ScrFI SfaNI SfcI SfoI SmaI
SmlI Sse8387I StyI TaqI TfiI
TseI Tsp45I TspRI Tth111I TthHB8I
Van91I XhoII XmaCI XmaI