(Circular) (Six-base) MAPSORT of: pFN21AB7007.seq Check: 5983 from: 1 to: 8977 pFN21AB7007 8977 bp With 182 enzymes: SgfI * January 16, 2009 15:25 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2163 2927 4011 4345 4660 4675 5295 2163 Size: 764 1084 334 315 15 620 5845 Fragments arranged by size: 5845 1084 764 620 334 315 15 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 6787 278 Size: 53 83 186 936 5251 2468 Fragments arranged by size: 5251 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3033 4863 5631 5865 6320 1937 Size: 1096 1830 768 234 455 4594 Fragments arranged by size: 4594 1830 1096 768 455 234 Acc65I G'GTAC_C Cuts at: 2955 6298 2955 Size: 3343 5634 AclI AA'CG_TT Cuts at: 1563 7104 7477 1563 Size: 5541 373 3063 Fragments arranged by size: 5541 3063 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2310 2843 3641 4512 5360 5369 5553 7040 Size: 678 533 798 871 848 9 184 1487 Cuts at: 7040 8424 8692 1632 Size: 1384 268 1917 Fragments arranged by size: 1917 1487 1384 871 848 798 678 533 268 184 9 AfeI AGC'GCT Cuts at: 4629 4629 Size: 8977 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 8780 AflIII A'CryG_T Cuts at: 1203 3177 3272 3976 4539 5201 7876 8853 1203 Size: 1974 95 704 563 662 2675 977 1327 Fragments arranged by size: 2675 1974 1327 977 704 662 563 95 AgeI A'CCGG_T Cuts at: 7808 7808 Size: 8977 AhdI GACnn_n'nnGTC Cuts at: 5872 5959 7706 5872 Size: 87 1747 7143 Fragments arranged by size: 7143 1747 87 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 8977 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2162 2359 4259 6038 1184 Size: 978 197 1900 1779 4123 Fragments arranged by size: 4123 1900 1779 978 197 AlwNI CAG_nnn'CTG Cuts at: 2320 2404 2494 2572 3143 3671 4079 5486 5749 Size: 84 90 78 571 528 408 1407 263 Cuts at: 5749 6720 8292 2320 Size: 971 1572 3005 Fragments arranged by size: 3005 1572 1407 971 571 528 408 263 90 84 78 ApaI G_GGCC'C Cuts at: 2675 3721 5278 5839 2675 Size: 1046 1557 561 5813 Fragments arranged by size: 5813 1557 1046 561 ApaLI G'TGCA_C Cuts at: 2310 7034 8190 2310 Size: 4724 1156 3097 Fragments arranged by size: 4724 3097 1156 ApoI r'AATT_y Cuts at: 1493 2370 3515 4181 4988 5363 5464 5648 6286 Size: 877 1145 666 807 375 101 184 638 Cuts at: 6286 6512 6664 8773 1493 Size: 226 152 2109 1697 Fragments arranged by size: 2109 1697 1145 877 807 666 638 375 226 184 152 101 AseI AT'TA_AT Cuts at: 160 1032 7531 160 Size: 872 6499 1606 Fragments arranged by size: 6499 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 8977 AvaI C'yCGr_G Cuts at: 1870 1943 2586 3394 4591 5277 5832 6302 1870 Size: 73 643 808 1197 686 555 470 4545 Fragments arranged by size: 4545 1197 808 686 643 555 470 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2947 2980 3543 3576 2947 Size: 33 563 33 8348 Fragments arranged by size: 8348 563 33 33 BamHI G'GATC_C Cuts at: 3337 6307 6674 3337 Size: 2970 367 5640 Fragments arranged by size: 5640 2970 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2497 2653 2720 2955 3466 Size: 325 217 627 710 156 67 235 511 Cuts at: 3466 4842 6298 7753 618 Size: 1376 1456 1455 1842 Fragments arranged by size: 1842 1456 1455 1376 710 627 511 325 235 217 156 67 BanII G_rGCy'C Cuts at: 1391 2526 2675 3371 3711 3721 3755 3898 3926 Size: 1135 149 696 340 10 34 143 28 Cuts at: 3926 5278 5589 5802 5839 5985 6296 1391 Size: 1352 311 213 37 146 311 4072 Fragments arranged by size: 4072 1352 1135 696 340 311 311 213 149 146 143 37 34 28 10 BbeI G_GCGC'C Cuts at: 2724 2724 Size: 8977 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2039 3084 928 Size: 971 140 1045 6821 Fragments arranged by size: 6821 1045 971 140 BbvCI CC'TCA_GC Cuts at: 2397 5750 6039 2397 Size: 3353 289 5335 Fragments arranged by size: 5335 3353 289 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 7168 7202 1903 Size: 34 5231 34 3678 Fragments arranged by size: 5231 3678 34 34 BciVI GTATCCnnnnn_n' Cuts at: 6869 8079 6869 Size: 1210 7767 BclI T'GATC_A Cuts at: 1548 1548 Size: 8977 BfrBI ATG'CAT Cuts at: 4118 4118 Size: 8977 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2670 4608 7588 Size: 107 122 71 631 605 998 1938 2980 Cuts at: 7588 136 Size: 1525 Fragments arranged by size: 2980 1938 1525 998 631 605 122 107 71 BglII A'GATC_T Cuts at: 5109 8972 5109 Size: 3863 5114 BlpI GC'TnA_GC Cuts at: 3194 6394 3194 Size: 3200 5777 Bme1580I G_kGCm'C Cuts at: 1790 2174 2314 2675 3721 4845 5001 5278 5839 Size: 384 140 361 1046 1124 156 277 561 Cuts at: 5839 7038 8194 1790 Size: 1199 1156 2573 Fragments arranged by size: 2573 1199 1156 1124 1046 561 384 361 277 156 140 BmgBI CAC'GTC Cuts at: 1318 2802 4440 4452 4540 1318 Size: 1484 1638 12 88 5755 Fragments arranged by size: 5755 1638 1484 88 12 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3091 3369 6708 Size: 416 53 83 399 24 1674 278 3339 Cuts at: 6708 7666 442 Size: 958 1753 Fragments arranged by size: 3339 1753 1674 958 416 399 278 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 8977 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2319 4942 5710 6004 2319 Size: 2623 768 294 5292 Fragments arranged by size: 5292 2623 768 294 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2621 3023 3391 4146 4912 4995 5261 5279 Size: 1382 402 368 755 766 83 266 18 Cuts at: 5279 7637 1239 Size: 2358 2579 Fragments arranged by size: 2579 2358 1382 766 755 402 368 266 83 18 Bpu10I CC'TnA_GC Cuts at: 2397 5750 5856 6039 2397 Size: 3353 106 183 5335 Fragments arranged by size: 5335 3353 183 106 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2729 3940 7102 7967 8265 2729 Size: 1211 3162 865 298 3441 Fragments arranged by size: 3441 3162 1211 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4513 7640 882 Size: 617 3014 3127 2219 Fragments arranged by size: 3127 3014 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3273 6768 493 Size: 711 2069 3495 2702 Fragments arranged by size: 3495 2702 2069 711 BsaBI GATnn'nnATC Cuts at: 3528 8971 3528 Size: 5443 3534 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2721 3024 6784 7166 Size: 53 83 186 936 1188 303 3760 382 Cuts at: 7166 7801 275 Size: 635 1451 Fragments arranged by size: 3760 1451 1188 936 635 382 303 186 83 53 BsaWI w'CCGG_w Cuts at: 4300 7410 7808 8082 8229 8640 4300 Size: 3110 398 274 147 411 4637 Fragments arranged by size: 4637 3110 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3220 3250 4714 4744 4949 Size: 30 165 30 1817 30 1464 30 205 Cuts at: 4949 4979 6072 6102 1178 Size: 30 1093 30 4053 Fragments arranged by size: 4053 1817 1464 1093 205 165 30 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2216 2245 2726 2984 2987 3008 3379 3624 Size: 1030 29 481 258 3 21 371 245 Cuts at: 3624 3779 5099 5258 5315 5333 5336 5339 5357 Size: 155 1320 159 57 18 3 3 18 Cuts at: 5357 5645 5800 5912 1186 Size: 288 155 112 4251 Fragments arranged by size: 4251 1320 1030 481 371 288 258 245 159 155 155 112 57 29 21 18 18 3 3 3 BseYI C'CCAG_C Cuts at: 2033 2271 3739 4582 4624 8180 8676 2033 Size: 238 1468 843 42 3556 496 2334 Fragments arranged by size: 3556 2334 1468 843 496 238 42 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2074 2135 3930 3935 4450 4502 4752 Size: 573 338 61 1795 5 515 52 250 Cuts at: 4752 4917 5001 5226 5273 1163 Size: 165 84 225 47 4867 Fragments arranged by size: 4867 1795 573 515 338 250 225 165 84 61 52 47 5 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 6427 7188 8216 1540 Size: 275 186 4426 761 1028 2301 Fragments arranged by size: 4426 2301 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2314 2526 4923 5286 7038 7123 8194 2314 Size: 212 2397 363 1752 85 1071 3097 Fragments arranged by size: 3097 2397 1752 1071 363 212 85 BsmI GAATG_Cn' Cuts at: 4372 6488 6581 4372 Size: 2116 93 6768 Fragments arranged by size: 6768 2116 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2174 2314 2526 2675 3371 3711 3721 Size: 399 384 140 212 149 696 340 10 Cuts at: 3721 3755 3898 3926 4845 4923 5001 5278 5286 Size: 34 143 28 919 78 78 277 8 Cuts at: 5286 5589 5802 5839 5985 6296 7038 7123 8194 Size: 303 213 37 146 311 742 85 1071 Cuts at: 8194 1391 Size: 2174 Fragments arranged by size: 2174 1071 919 742 696 399 384 340 311 303 277 213 212 149 146 143 140 85 78 78 37 34 28 10 8 BspEI T'CCGG_A Cuts at: 8640 8640 Size: 8977 BspHI T'CATG_A Cuts at: 6866 8596 6866 Size: 1730 7247 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2163 2927 4011 4345 4660 4675 5295 6332 Size: 1319 764 1084 334 315 15 620 1037 Cuts at: 6332 844 Size: 3489 Fragments arranged by size: 3489 1319 1084 1037 764 620 334 315 15 BsrBI CCG'CTC Cuts at: 1394 1823 6864 1394 Size: 429 5041 3507 Fragments arranged by size: 5041 3507 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3888 4333 7472 7646 65 Size: 1183 2640 445 3139 174 1396 Fragments arranged by size: 3139 2640 1396 1183 445 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2282 7621 7808 1700 Size: 110 472 5339 187 2869 Fragments arranged by size: 5339 2869 472 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 8977 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 8977 BssSI C'ACGA_G Cuts at: 2341 2525 7037 8049 2341 Size: 184 4512 1012 3269 Fragments arranged by size: 4512 3269 1012 184 BstAPI GCAn_nnn'nTGC Cuts at: 794 4694 4968 794 Size: 3900 274 4803 Fragments arranged by size: 4803 3900 274 BstBI TT'CG_AA Cuts at: 7781 7781 Size: 8977 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 8977 BstXI CCAn_nnnn'nTGG Cuts at: 4612 4612 Size: 8977 BstYI r'GATC_y Cuts at: 1972 3337 5109 6307 6674 7059 7076 8528 8614 Size: 1365 1772 1198 367 385 17 1452 86 Cuts at: 8614 8626 8972 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1772 1452 1365 1198 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2124 2190 3970 4605 513 Size: 552 1059 66 1780 635 4885 Fragments arranged by size: 4885 1780 1059 635 552 66 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 3258 487 Size: 683 56 2032 6206 Fragments arranged by size: 6206 2032 683 56 BtsI GCAGTG_nn' Cuts at: 2293 2362 2929 3947 4828 6493 7287 7307 2293 Size: 69 567 1018 881 1665 794 20 3963 Fragments arranged by size: 3963 1665 1018 881 794 567 69 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 8977 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2783 2818 529 Size: 35 2219 35 6688 Fragments arranged by size: 6688 2219 35 35 DraI TTT'AAA Cuts at: 3094 6281 3094 Size: 3187 5790 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3029 3293 7984 809 Size: 507 1713 264 4691 1802 Fragments arranged by size: 4691 1802 1713 507 264 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2640 3564 3907 Size: 54 1425 50 275 18 810 924 343 Cuts at: 3907 4434 4782 6424 7313 8942 8 Size: 527 348 1642 889 1629 43 Fragments arranged by size: 1642 1629 1425 924 889 810 527 348 343 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 6424 1537 Size: 275 4612 4090 Fragments arranged by size: 4612 4090 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3008 4128 5334 5554 6907 1353 Size: 1655 1120 1206 220 1353 3423 Fragments arranged by size: 3423 1655 1353 1206 1120 220 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2399 2603 7564 7938 8084 387 Size: 1064 138 810 204 4961 374 146 1280 Fragments arranged by size: 4961 1280 1064 810 374 204 146 138 EcoICRI GAG'CTC Cuts at: 2524 2524 Size: 8977 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2310 2621 2843 3023 3391 3641 4146 Size: 393 678 311 222 180 368 250 505 Cuts at: 4146 4512 4912 4995 5261 5279 5360 5369 5553 Size: 366 400 83 266 18 81 9 184 Cuts at: 5553 7040 7637 8424 8692 1239 Size: 1487 597 787 268 1524 Fragments arranged by size: 1524 1487 787 678 597 505 400 393 368 366 311 268 266 250 222 184 180 83 81 18 9 EcoNI CCTnn'n_nnAGG Cuts at: 2708 2729 4530 5297 5660 2708 Size: 21 1801 767 363 6025 Fragments arranged by size: 6025 1801 767 363 21 EcoO109I rG'GnC_Cy Cuts at: 2548 2671 3717 3718 5274 2548 Size: 123 1046 1 1556 6251 Fragments arranged by size: 6251 1556 1046 123 1 EcoRI G'AATT_C Cuts at: 4988 6286 6664 4988 Size: 1298 378 7301 Fragments arranged by size: 7301 1298 378 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2261 3255 5077 2261 Size: 994 1822 6161 Fragments arranged by size: 6161 1822 994 FspI TGC'GCA Cuts at: 2981 7483 2981 Size: 4502 4475 HaeII r_GCGC'y Cuts at: 1678 2724 4631 4806 8124 1678 Size: 1046 1907 175 3318 2531 Fragments arranged by size: 3318 2531 1907 1046 175 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3446 3478 4747 4779 4898 Size: 32 135 32 1874 32 1269 32 119 Cuts at: 4898 4930 5096 5128 5807 5839 5858 5890 5945 Size: 32 166 32 679 32 19 32 55 Cuts at: 5945 5977 6072 6104 6213 6245 7618 7650 7692 Size: 32 95 32 109 32 1373 32 42 Cuts at: 7692 7724 1373 Size: 32 2626 Fragments arranged by size: 2626 1874 1373 1269 679 166 135 119 109 95 55 42 32 32 32 32 32 32 32 32 32 32 32 32 32 19 HincII GTy'rAC Cuts at: 669 1657 1938 3034 3072 3123 5134 5632 5866 Size: 988 281 1096 38 51 2011 498 234 Cuts at: 5866 5911 6091 6321 6567 669 Size: 45 180 230 246 3079 Fragments arranged by size: 3079 2011 1096 988 498 281 246 234 230 180 51 45 38 HindIII A'AGCT_T Cuts at: 748 3858 5054 748 Size: 3110 1196 4671 Fragments arranged by size: 4671 3110 1196 HpaI GTT'AAC Cuts at: 3072 3123 6567 3072 Size: 51 3444 5482 Fragments arranged by size: 5482 3444 51 KasI G'GCGC_C Cuts at: 2720 2720 Size: 8977 KpnI G_GTAC'C Cuts at: 2959 6302 2959 Size: 3343 5634 MfeI C'AATT_G Cuts at: 6099 6576 6099 Size: 477 8500 MluI A'CGCG_T Cuts at: 5201 8853 5201 Size: 3652 5325 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5427 8091 8275 1305 Size: 4122 2664 184 2007 Fragments arranged by size: 4122 2664 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 3566 3909 4436 10 Size: 54 1425 343 1734 343 527 4551 Fragments arranged by size: 4551 1734 1425 527 343 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2341 3969 3975 6935 7294 7453 518 Size: 648 1175 1628 6 2960 359 159 2042 Fragments arranged by size: 2960 2042 1628 1175 648 359 159 6 MspA1I CmG'CkG Cuts at: 1606 2404 2470 2560 2629 4848 4911 5015 5371 Size: 798 66 90 69 2219 63 104 356 Cuts at: 5371 5938 6393 7070 8218 8463 1606 Size: 567 455 677 1148 245 2120 Fragments arranged by size: 2219 2120 1148 798 677 567 455 356 245 104 90 69 66 63 NaeI GCC'GGC Cuts at: 2284 2284 Size: 8977 NarI GG'CG_CC Cuts at: 2721 2721 Size: 8977 NcoI C'CATG_G Cuts at: 513 1065 2124 3970 513 Size: 552 1059 1846 5520 Fragments arranged by size: 5520 1846 1059 552 NdeI CA'TA_TG Cuts at: 387 5571 387 Size: 5184 3793 NgoMIV G'CCGG_C Cuts at: 2282 2282 Size: 8977 NheI G'CTAG_C Cuts at: 1052 1052 Size: 8977 NotI GC'GGCC_GC Cuts at: 6424 6424 Size: 8977 NsiI A_TGCA'T Cuts at: 4120 4120 Size: 8977 NspI r_CATG'y Cuts at: 1132 1229 2340 3070 3181 3202 3980 5455 6335 Size: 97 1111 730 111 21 778 1475 880 Cuts at: 6335 7880 1132 Size: 1545 2229 Fragments arranged by size: 2229 1545 1475 1111 880 778 730 111 97 21 PasI CC'CwG_GG Cuts at: 1348 3713 1348 Size: 2365 6612 PciI A'CATG_T Cuts at: 3177 3976 7876 3177 Size: 799 3900 4278 Fragments arranged by size: 4278 3900 799 PflMI CCAn_nnn'nTGG Cuts at: 1108 2494 2572 1108 Size: 1386 78 7513 Fragments arranged by size: 7513 1386 78 PfoI T'CCnGG_A Cuts at: 5295 5295 Size: 8977 PmeI GTTT'AAAC Cuts at: 6281 6281 Size: 8977 PmlI CAC'GTG Cuts at: 3273 3273 Size: 8977 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 7046 7078 8559 8591 7046 Size: 32 1481 32 7432 Fragments arranged by size: 7432 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2548 2548 Size: 8977 PshAI GACnn'nnGTC Cuts at: 1613 3252 1613 Size: 1639 7338 PsiI TTA'TAA Cuts at: 4187 5168 6547 4187 Size: 981 1379 6617 Fragments arranged by size: 6617 1379 981 PspOMI G'GGCC_C Cuts at: 2671 3717 5274 5835 2671 Size: 1046 1557 561 5813 Fragments arranged by size: 5813 1557 1046 561 PstI C_TGCA'G Cuts at: 830 2357 2409 2670 2924 2952 3254 4342 4823 Size: 1527 52 261 254 28 302 1088 481 Cuts at: 4823 5623 6329 830 Size: 800 706 3478 Fragments arranged by size: 3478 1527 1088 800 706 481 302 261 254 52 28 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 8977 PvuII CAG'CTG Cuts at: 2404 2560 2629 4911 5015 5938 2404 Size: 156 69 2282 104 923 5443 Fragments arranged by size: 5443 2282 923 156 104 69 RsrII CG'GwC_CG Cuts at: 2386 2386 Size: 8977 SacI G_AGCT'C Cuts at: 2526 2526 Size: 8977 SalI G'TCGA_C Cuts at: 1936 3032 5630 5864 6319 1936 Size: 1096 2598 234 455 4594 Fragments arranged by size: 4594 2598 1096 455 234 SanDI GG'GwC_CC Cuts at: 2548 2548 Size: 8977 SbfI CC_TGCA'GG Cuts at: 2670 6329 2670 Size: 3659 5318 ScaI AGT'ACT Cuts at: 4967 7225 8937 4967 Size: 2258 1712 5007 Fragments arranged by size: 5007 2258 1712 SfcI C'TryA_G Cuts at: 826 1045 2353 2405 2666 2920 2948 3223 3250 Size: 219 1308 52 261 254 28 275 27 Cuts at: 3250 4338 4819 5619 6325 7460 8141 8332 826 Size: 1088 481 800 706 1135 681 191 1471 Fragments arranged by size: 1471 1308 1135 1088 800 706 681 481 275 261 254 219 191 52 28 27 SfiI GGCCn_nnn'nGGCC Cuts at: 2670 2670 Size: 8977 SfoI GGC'GCC Cuts at: 2722 2722 Size: 8977 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 8977 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 8977 SmaI CCC'GGG Cuts at: 1872 5279 5834 6304 1872 Size: 3407 555 470 4545 Fragments arranged by size: 4545 3407 555 470 SmlI C'TyrA_G Cuts at: 820 1017 1943 2586 2708 3919 7081 7982 8244 Size: 197 926 643 122 1211 3162 901 262 Cuts at: 8244 820 Size: 1553 Fragments arranged by size: 3162 1553 1211 926 901 643 262 197 122 SnaBI TAC'GTA Cuts at: 493 6768 493 Size: 6275 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 8977 SphI G_CATG'C Cuts at: 1132 2340 3202 6335 1132 Size: 1208 862 3133 3774 Fragments arranged by size: 3774 3133 1208 862 SspI AAT'ATT Cuts at: 5 52 4272 6176 6901 5 Size: 47 4220 1904 725 2081 Fragments arranged by size: 4220 2081 1904 725 47 StuI AGG'CCT Cuts at: 1517 2351 1517 Size: 834 8143 StyI C'CwwG_G Cuts at: 513 1065 2124 2133 2643 2758 3970 5661 5805 Size: 552 1059 9 510 115 1212 1691 144 Cuts at: 5805 6416 513 Size: 611 3074 Fragments arranged by size: 3074 1691 1212 1059 611 552 510 144 115 9 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2155 2297 7004 7021 7174 7359 7804 Size: 1255 575 142 4707 17 153 185 445 Cuts at: 7804 325 Size: 1498 Fragments arranged by size: 4707 1498 1255 575 445 185 153 142 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4965 7223 8935 Size: 274 80 33 51 1443 2988 2258 1712 Cuts at: 8935 96 Size: 138 Fragments arranged by size: 2988 2258 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3220 3252 4191 4223 5546 5578 8937 8969 3220 Size: 32 939 32 1323 32 3359 32 3228 Fragments arranged by size: 3359 3228 1323 939 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 5627 5845 6086 1316 Size: 4311 218 241 4207 Fragments arranged by size: 4311 4207 241 218 XbaI T'CTAG_A Cuts at: 6313 6313 Size: 8977 XhoI C'TCGA_G Cuts at: 1943 2586 1943 Size: 643 8334 XmaI C'CCGG_G Cuts at: 1870 5277 5832 6302 1870 Size: 3407 555 470 4545 Fragments arranged by size: 4545 3407 555 470 XmnI GAAnn'nnTTC Cuts at: 2789 3155 4308 5055 7106 2789 Size: 366 1153 747 2051 4660 Fragments arranged by size: 4660 2051 1153 747 366 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 6785 276 Size: 53 83 186 936 5251 2468 Fragments arranged by size: 5251 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BsiWI BsmBI BstZ17I Bsu36I DraIII EcoRV FseI FspAI NruI PacI PspXI PsrI SacII SapI SexAI SrfI SwaI XcmI